Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

Thiamine결핍 유도후 쥐 전뇌의 성상교세포 Glial Fibrillary Acidic Protein(GFAP) 면역화학 활성도에 대한 변화

김승현,김희태,김주한,김명호,황세진,김명권,백태경 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

퍼지 전문가 시스템을 이용한 BLDC 전동기의 자동조정 PID 속도제어에 관한 연구

진광식,윤태성,권세현 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

This paper proposes the rule-based expert system with auto-turning of PID controller gain and shows the efficiency of fuzzy expert system for the speed control of BLDC motor. The PID controller gain tuned using RZN(refined ZN) formula to initial tuning and fuzzy expert system to subsequent tuning. The fuzzy expert PID control method proposed in this paper results in the reduction of overshoot, the rapid response and the robustness for disturbances and noise.

콘크리트 構造物의 混合모드 龜裂傳播모델(第1報)

陳致燮,張熙碩,崔顯泰,嚴長燮 부산대학교 공과대학 1989 硏究報告 Vol.38 No.-

This paper attempts to extract the crack tip stress intensity factors and to make a crack propagation model under mixed mode, from the finite element analysis of the concrete structures. In finite element analysis, all elements are initially assumed to be conventional quadratic isoparametric elements and are replaced by 6 node triangular singularity elements in the crack tip zone only. The stress intensity factors and the angle of propagation are carried out by displacement correlation technique and maximum circumferential tensile stress theory respectively. At the first part in a series of two papers, some numerical examples about the concrete SENB and DISK are presented to illustrate the practical effectiveness of the technique.

Thermus caldophilus GK24로부터 내열성 alkaline phosphatase의 특성

김유진,김현규,권석태 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Thermostable alkaline phosphatase was found from Thermus caldophilus GK24, extreme thermophile. T caldophilus GK24 alkaline phosphatase was found to be inducible. When starved of inorganic phosphate, T caldophilus GK24 produces the enzyme alkaline phosphatase. The addition of inorganic phosphate to growth medium had a repressive effect on enzyme synthesis. T caldophilus GK24 was tested for the production of alkaline phosphatase by the addition of various concentrations of sodium glutamate, bactotryptone, glucose and yeast extract to basal salts. Sodium glutamate was found to be effective for the alkaline phosphatase induction. The enzyme was stable in the pH range of 7-12. The optimum temperature, pH of the enzyme were 75-85℃ and 11.0-11.5, respectively.

자전거 에르고미터운동시 유ㆍ무산소성 역치수준의 근전도 변화

김기진,조현철,홍관이,정정진,손태열 龍仁大學校 體育科學硏究所 1991 體育科學硏究論叢 Vol.1 No.1

The aim of present study is investigate the change of IEMG and PEMG in the aerobic, anaerobic threshold by ventilation an blood locate concentration. Subjects of this study is adult sedentary men of age 32.18±4.28yrs. Subjects performed an incremental test on the bicycle ergometer from an initial work load of 0.5KP(60 rpm) to exhaustion by 3-min steps of 0.5KP. The results of this study was as follows: 1. The levels of relative exercises load in the ventilation, lactate, PEMG and IEMG by aerobic threshold were appeared 39.88±15.62%, 39.66±13.05%, 34.31±13.74% and 36.69±13.05%, respectively. It was not significant difference between variables. 2. The levels of relative exercise load in the ventilation, lactate, PEMG and IEMG by aerobic threshold were appeared 70.02±7.72%, 65.69±8.82%, 64.61±8.65% and 69.64±9.76%, respectively. It was not significant difference between variables. In this results above, It is considered that analysis methods by non-linear increase point of PEMG and IEMG related to both respiratory gas variables, blood lactate accumulation type and muscle function, muscle fiber recruitment type for determination of aerobic and anaerobic threshold.

스마트폰 블루투스 통신을 사용한 로봇 제어

김영진;김광우;김가덕;윤호성;김태공;서재현 인제대학교 2012 仁濟論叢 Vol.27 No.1

SLIS(Software License Integrated Solution) is composed of the 6 parts, Server Agent, Web Server, Client Agent, Client Manager, Service, and Install Shield. For the most part of the project is developed based on MFC platform except for the one, Client Manager, which is built on DDK(Driver Development Kit) and ASP. NET platform. Client Agent program installed on a customer's computer is implemented in 14 different classes. Its main function is able to limit and uninstall the purchased software by using teminateProcess. The purchased softwares expiration dates on customer's computer is being monitored by both Server agent and Client software itself. Client Manager hides the process of Client Agent program which shouldn't be killed by manipulating Process List in Kernel through DDK. To do this, Client Manager is internally implanted and registered in Windows Service to be ran as a part of windows system.

제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

피로를 유발하는 등속성 수축시와 회복기 혈중젖산 및 외측광근의 EMG Power Specturm 변화

정정진,조현철,김기진,윤성원,오수일,홍관이,손태열 龍仁大學校 體育科學硏究所 1991 體育科學硏究論叢 Vol.1 No.1

To examine the change of blood lactate concentration, EMG power spectrum, and Torque during fatiguing isokinetic contraction and recovery phrase, 6 sprinters performed maximal isokinetic knee extension at angular velocity of 3.14 rad.sec??(180°sec??) to 75% decreasing point (25%PT) using CybexⅡ system. In exercise phase, there was significant correlation between the decrement of peak EMG and median frequency and that of torque, and decrement of torque was greater than that of EMG . In recovery phase, EMG was restituded as normal value rapidly in a minute, but torque was restituded more slowly, and blood lactate concentration increased gradually from 50%PT to 3 minute after recovery. As a result of this study, it is suggested that muscle fatique due to decreasing of metabolic substrate and accumulation of metabolic products in peripheral site of muscle, and that inhibitory factors act neuromuscular junction in the end of exercise from rapid restitution of EMG in recovery phase.