Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

1998년 분리된 이질균의 역학적 특성

이복권,강연호,김성한,유재연,전정훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Jung, K. J.,Lee, E. K.,Kim, S. J.,Song, C. W.,Maruyama, N.,Ishigami, A.,Kim, N. D.,Im, D. S.,Yu, B. P.,Chung, H. Y. Springer Science + Business Media 2015 Journal of molecular medicine Vol.93 No.3

<P>Recent studies on senescence marker protein-30 (SMP30) have shown that it has an important functional role in the aging process, but its precise participation in cellular works has not been fully determined. We hypothesize that SMP30 plays crucial roles in signaling processes by modulating the balance of protein tyrosine kinase (PTK)/protein tyrosine phosphatase (PTP) and in activating proinflammatory NF-kappa B. An experimental paradigm of gain and loss of SMP30 function was established using SMP30-overexpressed YPEN-1 cells (herein referred to as 'SMP30(+) cells') and SMP30 (Y/-) knockout mouse kidneys. The resulting data show that SMP30 expression suppressed oxidative stress-induced PTK/PTP dysregulation and PP1/2A inactivation in SMP30(+) cells, leading to the suppression of NF-kappa B activation. In the kidneys of SMP30 (Y/-) mice, SMP30 deficiency was found to induce NF-kappa B activation via the upstream signaling of NIK/IKK and MAPKs and to upregulate downstream NF-kappa B-responsive gene expression. In this study, we also demonstrate for the first time that SMP30 deficiency induced PTK activity in SMP30 (Y/-) kidneys, thereby significantly increasing the tyrosine phosphorylation of a catalytic subunit of PP2A (PP2Ac-Tyr307). Based on these findings, we propose that SMP30 involves NF-kappa B regulation through the PTK/PTP balance and that the age-related decrease of SMP30 causes NF-kappa B activation, which contributes to an exacerbation of the inflammatory process during aging. Key message SMP30-deficient mice induced a shorter lifespan and redox changes. Overexpression of SMP30 prevented oxidative stress insults. The depletion of SMP30 increased redox-related PTK/PTP imbalance and PP1/PP2A inactivation. The depletion of SMP30 caused an elevation of NF-kappa B-responsive inflammatory markers. SMP30 may be a potent inhibitory protein against oxidative stress and chronic inflammation.</P>

“Grafting-From” Polymerization inside a Polyelectrolyte Hollow-Capsule Microreactor

Choi, Won San,Park, Jeong-Ho,Koo, Hye Young,Kim, Ju-Young,Cho, B. K.,Kim, Dong-Yu WILEY-VCH Verlag 2005 Angewandte Chemie Vol.44 No.7

<B>Graphic Abstract</B> <P>Interior decorating: Polymerization of styrene sulfonate monomers on the inner surface of polyelectrolyte hollow capsules results in polymers having molecular weights an order of magnitude higher than those produced in solution. The shape of the capsules after polymerization can be manipulated by varying the styrene sulfonate content (see SEM images; a: 25 wt %, b: 40 wt %). <img src='wiley_img/14337851-2005-44-7-ANIE200460971-content.gif' alt='wiley_img/14337851-2005-44-7-ANIE200460971-content'> </P>

Strain path effects on the microstructure evolution and mechanical properties of Zr702

Cao, W.Q.,Yu, S.H.,Chun, Y.B.,Yoo, Y.C.,Lee, C.M.,Shin, D.H.,Hwang, S.K. Elsevier 2005 Materials science & engineering. properties, micro Vol.395 No.1

<P><B>Abstract</B></P><P>A commercial-purity Zr702 was grain-refined from 20μm to 0.2–0.5μm by equal channel angular pressing. Grain refinement was most evident in the first pass but was insignificant during the subsequent passes. Two microstructural characteristics evolved: a lamellar structure and an equiaxed subgrain structure during the route A pressing and the route B<SUB>C</SUB> pressing, respectively. Due to the rotation of specimen in between passes, two sets of geometrically necessary boundaries were formed during the route B<SUB>C</SUB> pressing. The mechanism of grain refinement during the route A pressing was evolution of high-angle geometrically necessary boundaries from the low-angled ones, while that during the route B<SUB>C</SUB> pressing was decomposition and rearrangement of pre-existing boundaries. The yield stress of severely deformed specimens increased with the reducing grain size according to a Hall–Petch relationship.</P>

A study on the high temperature-dependence of the electrical properties in a solution-deposited zinc-tin-oxide thin-film transistor operated in the saturation region

Yu, K. M.,Bae, B. S.,Jung, M.,Yun, E. J. 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol. No.

<P>We investigate the effects of high temperatures in the range of 292 - 393 K on the electrical properties of solution-processed amorphous zinc-tin-oxide (a-ZTO) thin-film transistors (TFTs) operated in the saturation region. The fabricated a-ZTO TFTs have a non-patterned bottom gate and top contact structure, and they use a heavily-doped Si wafer and SiO2 as a gate electrode and a gate insulator layer, respectively. In a-ZTO TFTs, the trap release energy (E (TR) ) was deduced by using Maxwell-Boltzmann statistics. The decreasing E (TR) toward zero with increasing gate voltage (the density of trap states (n (s) )) in the a-ZTO active layer can be attributed to a shift of the Fermi level toward the mobility edge with increasing gate voltage. The TFTs with low gate voltage (low n (s) ) exhibit multiple trap and release characteristics and show thermally-activated behavior. In TFTs with a high gate voltage (high n (s) ), however, we observe decreasing mobility and conductivity with increasing temperature at temperatures ranging from 303 to 363 K. This confirms that the E (TR) can drop to zero, indicating a shift of the Fermi level beyond the mobility edge. Hence, the mobility edge is detected at the cusp between thermally-activated transport and band transport.</P>

치즈 제조 부산물을 이용한 ELISA Blocking Agent의 개발에 관한 연구

유제현,송진욱,조흥찬,차광종,박범석,김응률,이종익,이중복,谷口孝喜 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

본 연구는 유청과 탈지분유를 blotto로 이용하여 ELISA 수행시 항원·항체반응에 비특이반응을 막는 Blocking agent를 탐색하기 위해 실험을 수행했다. MAb의 역가측정 결과 유의차가 인정되는 OD값이 0.3이상 검색된 희석배율은 1B2의 경우 819,200배, 6D4의 경우 1,638,400배, 15B10의 경우 204,800배이었다. 서로 다른 Blotto가 항원·항체반응에 미치는 영향을 각기다른 MAb를 이용하여 조사해 본 결과 MAb Yo-156, Yo-5는 Blocking agent로 표준적으로 사용하고 있는 BAS가 비교하여 group A 바이러스 7종 모두에 비슷한 정도의 발색을 일으켰으며, MAb G6는 G6 type바이러스 4종 모두에서 비슷한 정도의 발색을 일으켰고, MAb G10은 G10 type 바이러스 3종 모두에서 비슷한 정도의 발색을 일으켰다. 따라서 본 실험에 사용한 모든 종류의 blotto소재인, 탈지분유1(서울우유(협)), 탈지분유2((주)매일유업), 혼합탈지분유((주)매일유업), whey powder1(Sigma), whey powder2 ((주)삼익유가공), WPC34((주)환이상사), WPC800((주)후드피아), WPC8000 ((주)후드피아), WPI((주)환이상사)는 현재 Blocking agent로 사용하고 있는 BSA와 비교하여 유사하게 비특이 반응을 막아주는 것으로 밝혀져 경제적으로 보다 저렴한 blotto 소재로 이용이 가능하다는 사실을 확인하였다. Enzyme-Linked-Immuno-Sorbent-Assay (ELISA) has been frequently used for detection of interaction of antigen and antibody. Many efforts have been made to reduce nonspecific reaction of ELISA. Bovine serum albumin (BSA) is usually used for blocking the nonspecific reaction even thought it is expensive. Recently skin milk has been focused on blocking nonspecific reaction of ELISA. This study aimed to substitute expensive blocking agent of ELISA with cheap by-product of cheese manufacture. To evaluate the substitution of BSA with wheys made in different companies, ELISA was carried out using monoclonal antibodies (MAbs) against rotavirus. The blocking capacity of the whey was compared with that of the BSA. The commercial wheys showed the same activity as BSA when ELISA was performed using MAb YO-156, S2-37, and Yo-5. To further investigate whether the whey affects the detection specificity of MAb, the ELISA was carried out using type specific MAbs. MAb G6 specific to serotype G6 bound to only rotavirus serotype G6 such as NCDV, UK, JBR, and Jedong strain whereas MAb G10 specific to serotype G10 bound to serotype G10 rotavirus only, demonstrating that the whey a and no effect on the binding specificity of MAb with respect to antigen-antibody reaction. These data indicate that the whey may have no effect on specific interation of antigen-antibody and may be widely used for blocking substitute because of competitive priority of its price.

레지오넬라균의 병리적 특성에 관한 연구(Ⅲ) : Legionella pneumophila의 Protease에 대한 Nonradioactive Gene Probe제작 및 그 특성에 관한 연구 Preparation and charcterization of nonradioactive gene probe to the protease of Legionella pneumophila

박경석,이혜경,오희복,유재연,황규잠,이기홍,박영미아,이종호 국립보건연구원 1994 국립보건원보 Vol.31 No.1

Salmonella Typhi균의 분자역학적 연구

이복권,강연호,박미선,유재연,김성한,김준영,전형근 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Evaluation of phenolic compounds and antimicrobial activities in transgenic Codonopsis lanceolata plants via overexpression of the γ-tocopherol methyltransferase (γ-tmt) gene

Ghimire, B.K.,Seong, E.S.,Yu, C.Y.,Kim, S.H.,Chung, I.M. Bureau for Scientific Publications, Foundation for 2017 South African journal of botany : official journal Vol.109 No.-

<P>This study was carried out to investigate antimicrobial activities of leaf and root extracts of transgenic Codonopsis lanceolata with over-expressing gamma-tocopherol methyl transferase (gamma-tmt) gene. The antimicrobial activity was assessed using the agar disc diffusion method and minimum inhibitory concentration (MIC) method, and the extracts from the transgenic C. lanceolata were compared with those of the wild plants by using the in-vitro antimicrobial assays with five pathogenic bacteria and two yeast species. The results indicated that transgenic C. lanceolata overexpressing gamma-tmt possesses antimicrobial activity against both gram-positive and gram-negative bacteria. Transgenic lines showed increased antimicrobial activity, ranging from 1.90 +/- 0.50 to 5.50 +/- 0.77 mm in the leaf extract of transgenic C. lanceolata, compared to the extract from the control plants, ranging from 1.27 +/- 0.50 to 4.73 +/- 0.40 mm. Staphylococcus aureus was the most susceptible microorganism to transgenic leaf extracts with lowerMICs (500 mu g/mL) and higher growth inhibition zones (5.33mm). Leaf extract of transgenic plants showed antimicrobial activity against Salmonella typhimurium, Klebsiella pneumoniae, and Escherichia coli. However, transgenic root extract showed lower antimicrobial activity against all tested microorganisms, with MIC > 1000 mu g/mL. The a-tocopherol content of the plants was enhanced by the overexpression of (gamma-tmt) gene compared to control plant. We observed an increase in the phenolic compound concentration in transgenic leaves. The predominant phenolic compounds that increased in the transgenic leaf were chlorogenic acid, luteolin, benzoic acid, and apigenin. The antimicrobial activity of transgenic C. lanceolata was strongly correlated to the concentration of phytochemical compounds. These results may help to identify novel natural antimicrobial agents that could be used against infectious diseases. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved.</P>