Brassica 種들의 花뢰크기에 의한 小胞子 發育段階 및 胚發生 比較

장영석(Young Seok Jang),민경수(Kyung Soo Min),오용비(Yong Bee Oh),정동희(Dong Hee Chung) 한국육종학회 1997 한국육종학회지 Vol.29 No.4

The purpose of this study is to search for the identifing method that could separate buds with embryogenic potential from collected buds for increasing the efficiency of embryogenesis by microspore culture in Brassica species. It was investigated developmental stage of microspore by bud-size and ratio of petal/anther length and compared to amount of embryogenesis through isolated microspore culture from the buds with late-uninucleate stage. In Brasica napus, Lisandra of spring type showed bud-size of 3.2~4.1mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/ anther length ratio were 1/2~3/4. Hallayuchae of winter type rapeseed showed bud-size of 3.1~3.6mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/anther length ratio were 1/3~1/2. Two genotypes of B juncea had the same petal/anther length ratio as range of 1/2~3/4 with embryogenic microspores but their bud-size were some difference as bud-size of Young-sanpohwanggyeja were 2.9~4.0mm and bud-size of Hwanggyeja were 2.9~4.1mm. Also, two genotypes of B. campestris had the same petal/anther length ratio as range of 2/3~3/4 with embryogenic microspores but bud-sizes were some difference as bud-size of Hyakusai were 2.9~3.4mm and bud-size of Sosongchae were 2.9~3.2mm. In B. oleracea, the petal/anther length ratio with embryogenic microspores according to bud-size were showed range of 1/2~2/3 in all of two genotypes and bud-size of Broccoli were 2.4~3.2mm and bud-size of Cauliflower were 2.9~3.7mm. In the embryo yield per 100,000 microspores by isolated microspore culture from the buds with embryogenic microspores, Young-sanpohwanggyeja of B. juncea were 47 embryos and Hwanggyeja of B. juncea were 23 embryos, B. campestris of Hyakusai were 367 and Sosongchae were 123, B. oleracea of Broccoli were 678 and Cauliflower were 562. However in B. napus was showed much differences by the growth habit, the spring type of Lisandra were producted 7,047 embryos of which higher about 9.2 times than those of winter type Hallayuchae.

원발성 유방암에서 Sodium Iodide Symporter의 발현과 ^(99m)Tc-MIBI 유방스캔의 관계

석주원,김성장,곽희숙,이창훈,김인주,김용기,배영태,김동수 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.6

목적: hNIS는 갑상선 조직 외에 다른 조직들에서도 발현된다고 알려져 있다. 유방암 세포는 그런 조직들 중에 하나이며, 그에 의한 유방암의 방사성옥소치료의 가능성이 제시되고 있다. 본 연구에서는 유방암 조직에서 hNIS의 발현정도를 알아보고, ^99mTc-MIBI 유방스캔과 hNIS의 발현 정도와의 관계를 조사하여 보았다. 대상 및 방법: 본 연구에서는 유방암으로 수술을 시행했던 56명의 환자를 대상으로 하였다. hNIS의 발현은 면역조직화학염색에 의해서 평가되었으며, 그 결과를 ^99mTc-MIBI 유방스캔의 판정 결과와 비교하였다. 결과: 전체 56명의 환자에서 hNIS의 발현율은 41.1%였다. 병리학적 진단에 의해 침윤성관상피암종이었던 49명에서의 발현율은 42.9%, 관상피내암종이었던 7명에서의 발현율은 28.6%였다. ^99mTc-MIBI 유방스캔에서 국소섭취 소견이 관찰되었던 41명에서의 hNIS의 발현율은 31.7%였다. 비정상적인 섭취 소견이 관찰되지 않았던 15명의 환자에서의 hNIS의 발현율이 의미있게 높게 관찰되었다(66.7%, p>0.05). 결론: 유방암 환자에서의 hNIS의 발현율은 그다지 높지 않았다. ^99mTc-MIBI 유방스캔에서 섭취증가 소견이 없을 때 hNIS의 발현율이 더 높았다. Purpose: Human Na^+/I- symporter (hNIS) is known to be expressed in many tissues other than thyroid gland. The breast cancer cells are one of them and the possibility of radioiodine therapy in treatment of the breast cancer may be suggested. We investigated the expression rate of hNIS and the relationship between the expression of hNIS and the finding of 99mTc-MIBI scintimammography in the breast cancer. Materials and Methods: Surgically proved 56 patients with breast cancer were the subjects of this study. The expression of hNIS were evaluated by immunohistochemistry and the results were compared to the findings of 99mTc-MIBI scintimammography. Results: Overall expression rate of hNIS was 41.1% in 56 patients. According to the pathologic diagnosis, it was 42.9% in 49 patients with invasive ductal carcinoma and 28.6% in the 7 patients with ductal carcinoma in situ. The expression rate of hNIS in the 41 cases with a focal increased uptake at he breast lesion on 99mTc-MIBI scintimammogram was 31.7%. That in the 15 cases without any abnormal uptake on the scan was significantly higher(66.7%, p<0.05). Conclusion: The expression rate of hNIS in the patients with breast cancer was not so high. The rate was higher in the patients with no increased uptake at the breast lesion on 99mTc-MIBI scintimammography. (Korean J Nucl Med 2002;36;325-32)

연소에 의한 엔진 진동 특성에 관한 연구

장석영,박경석,오택열,백영남 慶熙大學校 1993 論文集 Vol.22 No.-

Generally, a knock sensor for a high power engine detects cylinder wall vibration and estimates pressure vibration in the combustion chamber. In this study, so combustion pressure detected by pressure tranducer and cylinder wall vibration detected by an accelation sensor are estimated and the difference of those two signals are anlayzed. These two signals are analysed by frequency analysis method and compared with theorical knocking mode in the combustion chamber of the spark ignition engine. Design parameters of the knock sensor are determined by these analysed data.

스포츠센터 관계마케팅 실행요인이 관계품질, 구매행동에 미치는 영향

장호성,석용근 龍仁大學校 體育科學硏究所 2008 體育科學硏究論叢 Vol.17 No.1

The meaning of this research work lies in providing useful basic resources which can be utilized in the strategic respect of internal marketing by investigating how sports center customer in relation to relationship marketing implement factors on relationship quality and purchasing behavior, and by identifying how their service orientation affect business performance taking service quality as parameter. Customer of sports centers in Chungnam region were selected as the subjects of this survey and 248 participants were sampled by Convenience Sampling Method. Resulting data were processed by Frequency Analysis, Exploratory Analysis, Correlation Analysis, Regression Analysis using SPSS 11.0 program. The results from these research methods and analyses revealed the followings: First, results from regression analysis of performance factors of relationship marketing and relations quality revealed that customer-relations orientation, responsiveness and incentive had significant influences on reliability, and that customer-relations orientation and responsiveness, on involvement. Second, results from regression analysis of performance factors of relationship marketing and purchase behavior exhibited that incentive had a significant influence on repurchase intention, and that responsiveness and incentive, on positive word of mouth. Third, results from regression analysis of relationship quality and purchase behavior revealed that there were significant influences of all variables including reliability and involvement on repurchase intention and positive word of mouth.

반도체 산업에서의 Enterprise Document Management Architecture 구현에 관한 연구

장현성,이영중,송하석,한영준,안정삼 한국경영과학회 2001 한국경영과학회 학술대회논문집 Vol.- No.2

A systematic construction and re-use of technology related to the product development and production has been the most important for the semiconductor industry dependent on process and equipment Therefore, numerous outputs in the form of paper has been produced in the process of information management ranging from the creation to recycling and disposal of technologies. In this research, the technology and documents necessary for the business management in the field of semiconductor manufacturing were classified in an effort to solve problems while the modeling of document management architecture at the enterprise level was performed by properly setting up the security system to prevent the unauthorized disclosure of the product development technology to the third parties. Especially, the product and process specification are designed in such a way as to ensure a real-time response in interface with the production system in order to shorten the development lead-time and improve the productivity. This paper is to discuss the modeling approach, the strategy to construct the system and its results.

소아 흉통의 원인질환에 따른 임상양상

장국찬,김은영,노영일,양은석,문경래,박영봉 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

목적: 소아에서의 흉통은 흔히 접하게 되며 일반적으로 좋은 경과를 보인다. 가장 흔한 원인으로 특발성, 골격계이상, 위장관계 질환 등이 다양하게 언급되고 있으며 감별진단이 쉽지 않아 여러 검사를 시행하게 된다. 자세한 병력청취와 진찰이 진단에 가장 중요하다고 하지만 통계적 의의를 보고한 바는 드물다. 이에 흉통 원인을 분류하고 각각의 임상양상을 파악하여, 병력 청취와 진찰소견의 감별진단에 대한 유용성과 시행 검사들의 의의를 알아보고자 하였다. 방법: 2004년 3월 부터 2007년 8월 까지 조선대학교 병원 소아청소년과에 흉통을 주소로 방문한 환아 77명을 대상으로 하였다. 연령, 성별, 흉통의 위치, 지속시간, 양상, 빈도, 방문까지 걸린 시간, 동반증상, 유발인자의 각 항목과 원인 질환과의 관련성을 분석하였다. 흉부방사선, 심전도, 심초음파, 24시간 Holter, 운동부하 심전도, 상부 위장관 조영술, 24시간 pH 검사, 위내시경, 심근효소를 검사 하였다. 결과: 평균 나이는 10.2±2.8세로 6세에서 11세까지가 48명(62.3%)으로 가장 많은 분포를 보였고 남아 42명(54.5%), 여아 35명(45.5%)이었다. 흉통의 위치는 좌측이 32명(41.6%), 양상은 콕콕 찌르는 것이 27명(35.1%)으로 가장 많았고 지속시간은 1분미만이 36명(46.8%), 1-5분 사이가 28명(36.4%)으로 대부분 5분 미만이었다. 흉통 원인은 특발성이 27례(35.1%)로 가장 많았고, 위장관 질환 23례(29.9%), 늑골 연골염 12례(15.6%), 심혈관 질환 7례(9.1%), 정신신체화 질환 6례(7.8%), 폐질환 2례(2.6%)였다. 여아에서 연령이 높을수록 정신신체화 질환의 빈도가 높았다(P= 0.046). 흉골 하부 통증을 호소한 17명 중 13명(76.5%)이 위장관 질환으로 통계적으로 유의하였다(P= 0.023). 정신 신체화 질환은 6명에서, 위장관 질환도 20명(86.9%)에서 흉통지속시간이 5분 미만이었으나 통계적 의의는 없었다. 흉통 양상 중 타는 듯한 양상을 호소한 7명중 6명에서 위장관 질환이 원인이었으나 통계적 의의는 없었다. 선택 시행 검사 중 심초음파는 32명중 3례(9.4%), 24시간 Holter 검사는 13명중 5례(35.7%), 24시간 pH 검사는 28명 중 16례(57.1%)에서 이상소견을 보였다. 결론: 소아 흉통 환자 중 연령이 높은 여아에서는 정신 신체화 장애를, 흉골 하부 통증을 호소한 경우는 위장관 질환을 먼저 생각하는 것이 좋다고 판단된다. 흉통원인으로 위장관 질환의 빈도가 높고 시행검사들의 진단율을 볼때, 소아 흉통 환아 에서는 상부 위장관 조영술 등 위장관 질환의 검사를 기본적으로 시행하는 것이 좋을 것으로 생각된다. Purpose: Chest pain in children is usually benign. Chest pain in children results from a wide variety of causes. Differential diagnosis of causes is not easy and then many diagnostic approach is trying. Careful history and physical exams must guide the assessment of children with chest pain. The purpose of this study intends to evaluate children with chest pain and usefulness of careful history, physical examination and several diagnostic examinations. Methods: From March 2004 to August 2007, 77 patients presented with chest pain were enrolled. we evaluated age, sex, characteristics of chest pain (location, duration, description, frequency), accompanying symptoms, increasing reasons and relationship with cause of chest pain. Chest x-ray and ECG were checked in all patients. Cardiologic and gastrointestinal evaluations were checked when considered necessary. Results: The male and female ratio was 0.55:0.45, mean age 10.2 ± 2.8 year. Common ages of chest pain were 10 to 12 years old (38case, 49.3%). The location was on the left precordium in 32cases (41.6%) of the patients. The most common diagnostic findings of chest pain were idiopathic(27cases, 35.1%), gastrointestinal disorder (23cases, 29.9%), costo chondritis (12cases, 15.6%), cardiovascular disorder (7cases, 9.1%), psychosomatic disorder(6cases, 7.8%) and pulmonary disorder (2cases, 2.6%). Older children in female were more likely to have a psychosomatic cause (P= 0.046). The patients complained chest pain on substernal area were 17cases and 13cases (76.5%) were diagnosed as gastrointestinal disorder (P= 0.023). Positive findings of echocardio graphy, 24hr Holter ECG and 24hr pH monitoring were present in 3 (9.4%), 5 (35.7%) and 16 (57.1%) of the patients respectively. Conclusion: Gastrointestinal disorders are common causes and positive diagnostic rate of gastrointestinal evaluation is relatively high in children with chest pain. Old girls are more likely to have a psychosomatic disorder and substernal pain is the reliable sign to be considered as a gastrointestinal disease in children with chest pain. Hence, Diagnostic approach of gastrointestinal disease such as upper GI should be performed basically in mostly children with chest pain.

청정지역과 도시지역의 오존농도 특성 연구

장광미,강창희,서명석,허철구,박경윤,이호근,김영준 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

A study has been performed on the characteristics of rural and urban surface ozone concentration for the period of March 1992 to February 1993. The monitoring station of rural ozone is located at Kosan, Cheju and other urban monitoring stations are located at Seoul, Pusan and Kwangju. Rural's and urban's ozone data exhibit a distinct features in many ways. First, annual mean of rural ozone concentration is very high(42 ppbv) but urban's are very low(10~15 ppbv). Second, rural ozone data shows a seasonal variation with it's maximum in spring, and minimum in summer, but urban's show a seasonal variation with it's maximum in spring, and minimum in winter. Third, diurnal variation of rural data is very small but that of urban's are very large. Fourth, monthly mean of daily minimum of rural data is not low and shows a large seasonal variation, but those of urban's data are extremely low(<3 ppbv) and have no seasonal variations.

광대역 무선 가입자망(LMCS) 시스템의 상향스트림 처리를 위한 AIU(ATM Interface Unit)의 상향스트림 전송부 구현

박영민,박준호,장주석 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(A)

Local Multi-point Communication System (LMCS) is a broadband wireless telecommunications common carrier service in the 28 GHz range that will be capable of carrying basic and advanced communication services as "wireless" cable TV, Internet access, video teleconferencing, and various other multimedia. Signals from a central transmitting/receiving station will be sent to and received from homes and businesses within a four to five kilometer radius. Several such stations will be required in each city to service the total geographic area. Homes and businesses will send and receive the signals through equipment consisting of a small, unobtrusive antenna plus the associated electronics, which are about the size of a television converter. To use wireless technology is world-wide trend. In this paper, we implemented a system to generate and transmit up-stream frame in the LMCS system based on ATM cell. This control circuit is designed in VHDL and implemented using AT& T ORCA FPGA 2C08-208 pin chips. It actually serves perfect image through the internet with 8Mbps. This FPGA chip operates upto 30MHz input clock frequency.

순음의 위상차를 이용한 스테레오 시스템에서의 음상 정위 특성 연구

박영민,박양우,장주석 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(B)

In the AV system, stereophonic system has been studied to produce a realistic sound effect. The width of stereo AV system speakers is narrow, to have the spatial impression of sound effect, widening the sound image is necessary. The direction of sound image depends on the phase delay and the sound pressure level difference between two channels. In this paper, we analyze the relationship between the phase delay and the direction of the sound image relating to the frequency of sound source. Also we experimented to directionally localize the sound image of the pure tone with shifting phases and controling sound pressure level between two channels when the sound is reproduced by two speakers to make a spatial impression of sound effect.

Acid Phytase를 생산하는 Pseudomonas fragi의 분리와 phytase의 생산조건

김영진,장은석,인만진,오남순 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

cnrtkscP수가 방류되는 단위동물 밀집사육장 인근의 하천수로부터 phytase를 생산하는 세균들 중 활성이 가장 우수한 균주를 분리하여 Paseudomonas fragi Y9451로 동정, 명명하였다. 탄소원으로 fructose를 첨가할 경우에 phytase의 생산능이 가장 우수하였고, 배지에 7% 첨가할 경우에 72시간 배양 후 효소활성이 1,078mU/m로 최대의 phytase생산능을 보였다. 또한, 질소원으로는 nutrient broth(NB)가 적합하였으며, NB를 3%의 농도로 첨가할 경우에 96시간 배양 후 효소활성이 1,425mU/m까지 향상되었다. 인산원인 KH₂PO₄ phytate는 첨가 농도가 높을수록 phytase생산은 억제되었으며, 무기염으로 MgSO₄와 CaCI₂를 첨가하는 경우는 첨가농도와 상관없이 phytase의 생산을 현저히 억제하였다. 산업생산을 목적으로 하는 scale-up에 있어서의 통기교반 효과는 phytase의 생산량에 큰 영향을 미치는 것으로 나타났다. A bacterial strain producing a high level of an extracellular phytase was isolated from livestock waste water, identified as a strain of Pseudomonas fragi and designated as Pseudomonas fragi Y9451. Under the phytase production medium, the activity of phytase reached the highest level after 120 hours of incubation. On the effect of carbon sources on the phytase production, the most favorable carbon source for phytase production was fructose. As for the effect of nitrogen sources, high levels of phytase activity were detected in the medium containing nutrient broth as the nitrogen source. Free PO₄^(3-) inhibited phytase production with increasing concentration of KH₂PO₄ and phytate in the media. The addition of CaCl₂ and MgSO₄ also resulted in the inhibition of phytase production. To investigate the effect of aeration on the phytase production, different volumes of culture broth Erlenmeyer flasks were incubated in rotary shaker at the speed of 200 rpm. As a result, a high level of phytase activity was detected at small volume of culture broth at compared to larger volume because of its more aerobic condition.