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You-Kun Zheng,Cui-Ping Miao,Hua-Hong Chen,Fang-Fang Huang,Yu-Mei Xia,You-Wei Chen,Li-Xing Zhao 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy Panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing rootrot of P. notoginseng. Methods: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of P. notoginseng. Results: A total of 89 fungi were obtained from the roots, stems, leaves, and seeds of P. notoginseng, and 41 isolates representing different morphotypes were selected for taxonomic characterization. The fungal isolates belonged to Ascomycota (96.6%) and Zygomycota (3.4%). All isolates were classified to 23 genera and an unknown taxon belonging to Sordariomycetes. The number of isolates obtained from different tissues ranged from 12 to 42 for leaves and roots, respectively. The selected endophytic fungal isolates were challenged by the root-rot pathogens Alternaria panax, Fusarium oxysporum, Fusarium solani, Phoma herbarum, and Mycocentrospora acerina. Twenty-six of the 41 isolates (63.4%) exhibited activity against at least one of the pathogens tested. Conclusion: Our results suggested that P. notoginseng harbors diversified endophytic fungi that would provide a basis for the identification of new bioactive compounds, and for effective biocontrol of notoginseng root rot.
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Zheng, You-Kun,Miao, Cui-Ping,Chen, Hua-Hong,Huang, Fang-Fang,Xia, Yu-Mei,Chen, You-Wei,Zhao, Li-Xing The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3
Background: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy Panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of P. notoginseng. Methods: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of P. notoginseng. Results: A total of 89 fungi were obtained from the roots, stems, leaves, and seeds of P. notoginseng, and 41 isolates representing different morphotypes were selected for taxonomic characterization. The fungal isolates belonged to Ascomycota (96.6%) and Zygomycota (3.4%). All isolates were classified to 23 genera and an unknown taxon belonging to Sordariomycetes. The number of isolates obtained from different tissues ranged from 12 to 42 for leaves and roots, respectively. The selected endophytic fungal isolates were challenged by the root-rot pathogens Alternaria panax, Fusarium oxysporum, Fusarium solani, Phoma herbarum, and Mycocentrospora acerina. Twenty-six of the 41 isolates (63.4%) exhibited activity against at least one of the pathogens tested. Conclusion: Our results suggested that P. notoginseng harbors diversified endophytic fungi that would provide a basis for the identification of new bioactive compounds, and for effective biocontrol of notoginseng root rot.
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You, Jiangfeng,Liu, Xing,Zhang, Bo,Xie, Zhongkai,Hou, Zhiguang,Yang, Zhenming The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.1
Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humus and albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate the impact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0-5 cm, 5-10 cm, and 10-15 cm for different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with $NH_4Cl $(exchangeable Al) and Na-pyrophosphate (organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, $NH_4^+$, total organic carbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in the different-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils was positively correlated with the $NH_4^+$ (r=0.463, p<0.01), exchangeable calcium (r=0.325, p<0.01) and TOC (r= 0.292, p < 0.05) concentrations. The $NO_3^-$ showed remarkable surface accumulation (0-5 cm) in the summer and even more in the autumn but declined considerably the next spring. The exchangeable Al fluctuated from $0.10mg\;g^{-1}$ to $0.50mg\;g^{-1}$ for dry soils, which was positively correlated with the $NO_3^-$ (r=0.401, p < 0.01) and negatively correlated with the TOC (r=-0.329, p < 0.05). The Al saturation varied from 10% to 41% and was higher in the summer and autumn, especially in the 0-5 cmand 5-10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng beds with plastic shade.
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You Fu,Zesong Fei,Niwei Wang,Chengwen Xing,Lei Wan 한국산학기술학회 2013 SmartCR Vol.3 No.1
Deploying PicoStations in traditional Macro-cellular networks, especially in hot-spot areas, is a promising strategy to boost system throughput and enhance indoor coverage. However, PicoStations’ introduction brings some new problems, such as severe interference and energy consumption, which may result in performance degradation. In order to improve energy efficiency, throughput fairness and PicoStation coverage, a practical energy-efficient dense PicoStation deployment and power control algorithm is proposed. The proposed scheme maximizes both the number of active PicoStations that can achieve the required capacity and the sum cell capacity under individual capacity constraints in two steps. Finally, simulation results demonstrate that the proposed algorithm outperforms the traditional cell capacity maximum scheme in terms of user fairness and energy saving.
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The Study of Pilot Process of Separation and Purification of Catechin Using Wood Fiber Resin
Xing-hai Zhang,Jin-wei Xu,Yue-fei Wang,Yin Gao,You-ying Tu 한국차학회 2015 한국차학회지 Vol.- No.S
This paper studies the pilot production of tea polyphenol (TP) from membrane filtered tea extract using wood fiber resin. We extract, isolate and purify TP using ultrasonic wave extraction, ultrafiltration membrane and wood fiber resin column chromatography techniques. We then use high performance liquid chromatography (HPLC) to filter optimal parameters and get the final product using spray drying method. The results show that after above process the content of caffeine, EGCg and catechin in 40% eluent is 0.607%, 60.7%, 86.95% separately and the low caffeine high catechin procuct yield rate is 7.0%. While in 10% eluent the content of caffeine, EGCg and catechin is 26.6%, 16.5%, 23.7% separately, and the high caffeine low catechin product yield rate is 7.3%. So the total summed yield rate is 14.3%. The pilot process may provide reference for future green TP production.
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Face inpainting via Learnable Structure Knowledge of Fusion Network
You Yang,Sixun Liu,Bin Xing,Kesen Li 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.3
With the development of deep learning, face inpainting has been significantly enhanced in the past few years. Although image inpainting framework integrated with generative adversarial network or attention mechanism enhanced the semantic understanding among facial components, the issues of reconstruction on corrupted regions are still worthy to explore, such as blurred edge structure, excessive smoothness, unreasonable semantic understanding and visual artifacts, etc. To address these issues, we propose a Learnable Structure Knowledge of Fusion Network (LSK-FNet), which learns a prior knowledge by edge generation network for image inpainting. The architecture involves two steps: Firstly, structure information obtained by edge generation network is used as the prior knowledge for face inpainting network. Secondly, both the generated prior knowledge and the incomplete image are fed into the face inpainting network together to get the fusion information. To improve the accuracy of inpainting, both of gated convolution and region normalization are applied in our proposed model. We evaluate our LSK-FNet qualitatively and quantitatively on the CelebA-HQ dataset. The experimental results demonstrate that the edge structure and details of facial images can be improved by using LSK-FNet. Our model surpasses the compared models on L1, PSNR and SSIM metrics. When the masked region is less than 20%, L1 loss reduce by more than 4.3%.
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( Wan Xing Yong ),( Xu Cheng Fu ),( Lu Chao ),( Yu Wei Lai ),( Zhu Hua Tuo ),( Yu Chao Hui ),( Li You Ming ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Serum uric acid is strongly associated with nonalcoholic fatty liver disease (NAFLD) and insulin resistance in patients. However, whether this association is causally or coincidentally with NAFLD and insulin resistance remains uncertain, neither the mechanisms behind this association are unclear so far. Methods: We first analyzed the impact of uric acid on development of hepatic steatosis in mice and two cell models (HepG2 and HL7702), and then explored the effect of uric acid on insulin signaling, including phosphorylation of insulin receptor substrate 1 (IRS1) and Akt in HepG2 and HL7702 cells. Further, we studied the role of NLRP3 inflammasome in regulation of hepatic steatosis and insulin signaling. Results: Uric acid directly induced hepatocyte fat accumulation both in vivo and in vitro. Further, uric acid treatment decreased insulin-induced phospho-Akt (ser437) and enhanced the phospho-IRS1(ser307) in HepG2 and HL7702 cells. Then, we found significant increases of NLRP3 inflammasome-related molecules, both mRNA and protein levels, including NLPR3, caspase-1, IL-1ß, and IL-18, in HepG2 and HL7702 cells stimulated with uric acid. We also found that uric acid induced significant elevations of IL-1ß and IL-18 levels in culture supernatants of HepG2 and HL7702 cells. Consistent with in vitro results, mice fed 8 weeks of hyperuricemia-inducing diet resulted in significant up-regulation of hepatic mRNA and protein expressions of NLPR3, caspase-1, IL-1ß and IL-18, and elevation of serum IL-1ß and IL-18 levels. Further experiments indicated that silencing NLRP3 expression significantly alleviated uric acid-induced fat accumulation in vitro. Moreover, inhibition of NLRP3 expression ameliorated uric acid induced insulin signaling impairing, confirmed by increased insulin- induced phospho-Akt (ser437) and reduced the phospho-IRS1(ser307) in vitro. Conclusions: Our results suggest that uric acid contributes to hepatic steatosis and impairs insulin signaling through the NLRP3 inflammasome dependent mechanism.
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Jiangfeng You,Xing Liu,Bo Zhang,Zhongkai Xie,Zhiguang Hou,Zhenming Yang 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3
Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humus and albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate the impact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0-5 cm, 5-10 cm, and 10-15 cm for different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with NH₄Cl (exchangeable Al) and Na-pyrophosphate (organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, NH₄⁺, total organic carbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in the different-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils was positively correlated with the NH⁺₄ (r=0.463, p<0.01), exchangeable calcium (r=0.325, p<0.01) and TOC (r = 0.292, p < 0.05) concentrations. The NO₃ showed remarkable surface accumulation (0-5 cm) in the summer and even more in the autumn but declined considerably the next spring. The exchangeable Al fluctuated from 0.10 mg g<SUP>-1</SUP> to 0.50 mg g<SUP>-1</SUP> for dry soils, which was positively correlated with the NO₃ (r=0.401, p < 0.01) and negatively correlated with the TOC (r=-0.329, p < 0.05). The Al saturation varied from 10% to 41% and was higher in the summer and autumn, especially in the 0-5 cmand 5-10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng beds with plastic shade.
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