Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

전이금속과 질소 - 산소를 가진 거대고리 리간드와의 착물형성에 관한 연구

최규성,김정,김용규 慶南大學校 附設 基礎科學硏究所 1992 硏究論文集 Vol.4 No.-

The stability constants of Co(II), Ni(II), Cu(II) ions with macrocyclic ligands containing nitrogen and oxygen donors such as 1, 15-diaza-3, 4:12, 13-dibenzo-5, 8, 11-trioxacycloheptadecane(NEOD), 1, 12, 15-triaza-3, 4 : 9, 10-dibenzo-5, 8-dioxacycloheptadecane(NDOE) have been determined by UV-VIS spectrophotometric method in DMSO. The order of complex stability constants of the transition metal ions with ligands is Co(II) < Ni(II) < Cu(II), the same as the natural order of stability published by Williams-Irving, and stability constants have been found to be effected by the chelate effect of macrocyclic ligands, so the order of ligands effect is NEOD < NDOE.

비냉각 적외선 감지기용 비나듐 산화막의 전기전도기구

최용남,최복길,이규대 공주대학교 생산기술연구소 2001 論文集 Vol.9 No.-

Thin films of vanadium oxide(VO_(x)) have been deposited by r.f. magnetron sputtering from V_(2)O_(5) target in gas mixture of argon and oxygen. The oxygen/(oxygen+argon) partial pressure ratio is changed from 0% to 8%. Crystal structure, chemical composition and electrical properties of films sputter-deposited under different oxygen gas pressures have been characterized through XRD, AES, RBS and electrical conductivity measurements. All the films prepared below 8% O_(2) are amorphous, and those prepared without oxygen are gray indicating the presence of V_(2)O_(4) phase in the films. In order to measure the current-voltage characteristics and temperature dependence of conductance, devices of A1/VO_(x)/A1 sandwich structure have been fabricated. I-V characteristics are distinguished between linear and nonlinear region. In the low field region the conduction is due to Schottky emission, while at high field it changes to Fowler-Nordheim tunneling type conduction. The conductivity measurements have shown an Arrhenius dependence of the conductivity on the temperature.

淸州市의 住宅問題와 改善에 관한 硏究 : 需給과 環境을 中心으로 With special reference to its Demand-Supply and Environment

崔龍奎 청주대학교 사회과학연구소 1983 한국사회과학연구 Vol.1 No.-

This is a study on the housing environment and its problems with special reference to its demand and supply problem in this regional city. The city of Cheong-ju is generally known as an educational, cultural, and also well regarded as a good city to live in comfort. But the housing problem is one of serious problems in this city than in any other cities. It seems that resolving the problem in near future may not be possible but as the government has designated this city as one of core regional cities the housing problem in this city may be dealt with better priority. This paper looked into the houing demand and its supply and then pursued on the housing environment in order to find ways. to improve the situation in this seemingly stabilized city.

Semiclassical Analysis for O + Pb Elastic Scattering at E=216.6 and 312.6 Mev

최치규,현남규,김용주,홍성락 제주대학교 1992 논문집 Vol.34 No.-

실험실계에서의 입사핵?? 에너지가 216.6과 312.6 MeV이고 표적핵?? 인 탄성산란에 대한 실험데이타를 수정된 Glauber모델을 사용하여 분석하였다. 계산된 결과들은 실험데이타와 좋은 일치를 보여주었다. .

솔잎(갈비)·솔방울의 수처리에 관한 연구

최순규,엄기수,성현순,김윤영,이시우,정대일,이용균 동아대학교 환경문제연구소 1994 硏究報告 Vol.17 No.2

The absorption ability of low density heavy metal of pine needles and cones is better than one of existing absorption materials in treatment ability and experimental condition. The elimination ability of chlorine of pine needles and cones is better than one of active carbon. In elimination of trihalomethane, the effect of treatment of pine needles is lower than one of pine cones. And surface of pine needles and cones after absorption of heavy metal ion was observed by Scanning Electron Microscope(SEM). Application and availability of pine needles and cones as absorption material need lots of experiments. The experiment on Gal-Bi(fallen leaves) is proceeding.

6주간의 淸暑益氣湯 經口投與가 에너지대사 및 호르몬 변화에 미치는 영향

최용어,강명신,유종만,이규성,오재근,조준용 韓國體育大學校附屬 體育科學硏究所 1994 韓國體育大學校附屬 體育科學硏究所論文集 Vol.13 No.1

The purpose of this study was to investigate the effects of chung-seo-ik-gi -tang administration on the metabolic responses and hormones in 18 healthy-male elite boxes. All subjects were divided randomly with two groups, Chung-sea-ik-gi-tang Group(N=18) and placebo group(N=18) and perfored with 70% of HR max using bycicle ergometer unfil exhaustion. During the submaximal exercise, heart rate was maesured every two min. The blood samples were collected from antecubital vein by 10ml syranges(6ml/each) before immediately after exhaustion, recover-10 min, recover-20 min and recover-30 min. The samples were to analyzed for the level of glucose, lactate, TG, TC, HDL-C, insulin and catecholamine. The results of various blood parameters were summarized as follow; l. Energy substrates (plasma glucose, TG, TC, HDL-C) changes in group C were significantly different from group P. Plasma glucose in group C was significantly lowered at recover-10 min compared to group P (P<0.05). and also TG, TC and HDL-C in group C were significantly increased after 3 weeks compared to group P (P<0.05) whereas. Lactate accumulation in group C was significantly decreased after 3 weeks compared to group P (P<0.05). 2. Plasma insulin level was not showed significant difference between two groups. But insulin depression tendency after 3, 6 weeks in group C was allevated compared to group P. Increasing rate of norepinephrine in group C at Post-exhaustion after 3weeks lowered compared to group P. Norepinephrine in group C at rest periods after 3 weeks showed significant difference compared to group C. The results indicated that Chung-seo-ik-gi-tang had the effects of ergogenic aids on fatigue resistance and recovery from exhaustive exercise at the level of 70% HR max.

冬蟲夏草의 抗轉移와 免疫增進에 關한 硏究

최우진,유화승,이용연,서상훈,조정효,이연월,손창규,조종관 대전대학교 한방병원 2000 惠和醫學 Vol.9 No.2

This experimental study was carried out to evaluate the effects of Cordiceps sinensis on the antimetastasis and immune activity. In order to investigate the effect of Cordiceps sinensis, the followings were performed; Cytotoxicity, inhibition of MMP-2 & MMP-9 gene expression, fraction of CD4+, CD8+, CD19+ in splenic cell, the moprphological change of splenic cells, gene expression of IL-12(p35), IL-12(p40), IFN-?? and splenic cell proliferation by CS-E. The results were obtained as follows. 1. CS-E did not present cytotoxic effect on L+14, B16-F10 melanoma cells and HMCB. 2. CS-E inhibited MMP-2 and MMP-9 gene expression more effectively compared with control group. 3. CS-E inhibited invasion of B16-F10 melanoma cells more effectively compared with control group. 4. CS-E helped CD4+, CD8+, CD19+ expression more effectively compared with control group. 5. CS-E activated phagocytic cells in spleen more effectively compared with control group. 6. CS-E helped IL-12(p35), IL-12(p40), IFN-?? gene expression in splenic cell more effectively compared with control group. 7. CS-E activated splenic cell proliferation more effectively compared with control group. From above findings, it is suggested that CS-E is able to inhibit metastasis of cancer and activate immune response system.