리튬-필로카르핀 경련모델의 특징 : 행동양상, 뇌파, Fos단백의 발현 및 신경병리학적 변화 Behaviors, Electroencephalography, Fos Expression, and Neuropathologic changes

김영인,이광수,김범생,김용재,천명훈,김명석 대한신경과학회 1996 대한신경과학회지 Vol.14 No.1

복합성 치아종

노용관,김대범,백병주 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

The odontomas are most common odontogenic tumors composed of various tooth-like structures : enamel, dentin, cementum, pulp tissue. The etioglogy of the odontoma is not evident but it has been suggested that local trauma or infection may produced such a lesion. Clinically, they are asymptomatic, slow growing and lesions of limited growth that show no infitration to the adjacent tissues. They are dignosed most often in the second decade of life, and have no predilection for jaws. The treatment is surgical excision and there is no expectancy of recurrence or serious complications after removal.

알쯔하이머병 환자의 뇌혈류 장애 : Statistical Parametric Mapping을 이용한 Tc-99m ECD SPECT 분석 Statistical Parametric Mapping analysis of Tc-99m ECD SPECT

양동원,김범생,손영민,박정욱,정용안,손형선,김상윤 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.2

Background and Objectives . Alzheimer's disease (AD) is the most common cause of the dementia Pathologic and functional changes of AD are known to be unevenly distributed in the brain. This study investigates changes of cerebral blood flow (CBF) in patients with AD to determine brain regions with hypoperfusion that reflects progression of disease. Methods. Tc-99m-ECD single photon emission computed tomography (SPECT) was performed to measure regional CBF and statistical parametric mapping (SPM) 99 soft ware was used for the automated and objective approach to analyze SPECT image data. Twenty-five patients with mild to moderate dementia who met NINCDS-ADRDA critena for AD (male 11 female 14) and 17 age-matched normal control subjects were studied. The severity of AD was measured by clinical dementia rating (CDR) scale Results. SPM analysis of SPECT images revealed that CBF in the anterior cingulate gyrus left superior panetal cortex left medial frontal cortex left infenor temporal cortex. right thalamus and left hippocampus was significantly decreased in AD patients compared with normal controls (corrected p<0.05). The reduction of CBF according to CDR was localized in the left hippocampus and infenor temporal cortex right antenor and postenor ciagulate cortex and right superior panetal cortex (uncorrected p<0.01). Conclusions : Our data suggest that the cognitive dysfunction of AD is strongly related with functional abnormality in discrete brain areas 99m-Tc ECD SPECT with SPM analysis could be used as a useful functional imaging tool in AD study.

두경부에 발생한 방선균증 3예

홍석중,주준범,김용재,이봉재 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.11

장기적 트립토판 결여식이 생쥐의 유해감각예민도에 미치는 영향

서영대,박제민,김명정,전명호,양구범,장세헌,강철중,김정태,김용식 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

The depressive patients can be divided into two subgroups by presence and absence of pain symptoms. Those without pain symptoms have blunted pain sensitivity whereas those with pain have normal range of pain sensitivity, and are clinically characterized by anxiety and irritability. The aim of the study is to test if these clinical profiles of the depressives with pain symptoms are related with reduced level of brain serotonin(5-HT). Forty four mice were randomly divided into two groups : one group to be bred with tryptophan free diet and the other with normal control diet, each for 4 weeks. Before the beginning of the breeding period, measured were locomotor activity by open field test, anxiety by elevated plus maze and nociceptive sensitivity by tail flick test, before and after forced swimming(FS). During each FS, duration of immobilization was also measured. The sable sets of measurements were repeated at the end of the breeding period. The brain 5-HT and 5-hydroxyindoleacetic acid(5-HIAA) were measured by high performance liquid chromatography. The results were as follows 1.Four weeks of tryptophan free diet reduced significantly body weight, brain weight, and levels of 5-HT and 5-HIAA in the whole brain. 2.Tryptophan depletion did not influence basal nociceptive sensitivity as measured by tail flick latency before FS. The normal blunting of the pain sensitivity induced by forced swimming was preserved in the tryptophan depleted group. 3.Tryptophan depletion did not influence general locomotor activity in open field. 4.Tryptophan depletion increased significantly time spent on the open arms at the elevated plus maze test done before FS. This anxiolytic-like effect was reduced by FS. 5.Tryptophan depletion did not influence duration of immobilization during FS. From these results, it is suggested that the genesis of the depression with pain symptoms is not medicated by quantitative reduction of brain serotonin

Protective effects of a novel herbal decoction on focal cerebral ischemia in a rodent model

Kim, Myung-Gyou,Choi, Jae-Hwan,Lim, Jong-Pil,Kim, Dae-Keun,Shin, Tae-Yong,Boo, Yungmin,Kim, Sun-Yeou,Kim, Hocheol,Ha, Eunyoung,Park, Hun-Kuk,Kim, Jeongseon,Lim, Ha-Sup,Kim, Ee-Hwa,Kim, Jeung-Beum,Leem Informa UK (TaylorFrancis) 2007 Neurological research Vol.29 No.suppl1

감비수 향 흡입이 절식을 유도한 쥐의 시상하부에서 섭식관련 신경전달물질의 발현과 인지력에 미치는 영향

김동현(Dong-Hyun Kim),고일규(Il-Gyu Ko),김성은(Sung-Eun Kim),김지은(Ji-Eun Kim),강연호(Yeon-Ho Kang),김태운(Tae-Woon Kim),지은상(Eun-Sang Ji),김용민(Yong-Min Kim),김이화(Ee-Hwa Kim),김정범(Jeong-Beum Kim),김동희(Dong-Hee Kim),김창주(Cha 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

본 연구는 절식을 유도한 ICR계 생쥐에서 감비수 향 흡입이 시상하부에서 leptin과 neuropeptide Y (NPY)의 발현에 미치는 영향과 단기 기억력에 미치는 효과를 알아보고자 하였다. ICR 계열 수컷 쥐 24마리를 무작위 추출법에 의해 대조군, 절식군, 절식과 감비수 향 흡입군(각 군 n=8)의 3군으로 분류하였다. 실험결과, 절식은 시상하부에서 leptin의 발현을 감소시켰고 NPY의 발현은 증가시켰다. 또한, 절식에 의하여 단기 기억력과 해마에서의 신경성장인자인 BDNF의 발현이 감소되었다. 반면에, 절식 중 감비수 향을 흡입 시 시상하부에서 leptin의 발현은 증가되었고 NPY의 발현은 감소되었다. 그리고 감비수 향의 흡입에 의하여 단기 기억력이 향상 되었고 해마에서의 신경성장인자인 BDNF의 발현이 증가되었다. 본 실험 결과, 감비수 향 흡입은 절식 시 증가된 음식섭취에 대한 욕구를 억제할 수 있으며 절식에 의하여 감소된 단기 기억력을 향상시키는 효과가 있음을 알 수 있었다. In the present study, we investigated the effects of Gambisu inhalation on the expressions of neurotransmitters implicated in the food intake in the hypothalamus and cognition using starved mice. For this, the effects of Gambisu inhalation on the expressions of leptin and neuropeptide Y (NPY) in the paraventricular nucleus (PVN) of hypothalamus of starved mice were evaluated by immunohistochemistry. Short-term memory of the mice was also assessed using a passive avoidance task and brain-derived neurothrophic factor (BDNF) expression in the hippocampus was determined by Western blot analysis. ICR mice were divided into three groups: control group, starvation group, and starvation with Gambisu inhalation group. The mice in the food-deprivation group were restricted food for 48 h. The mice in the Gambisu inhalation group were exposed to inhale Gambisu for 60 min twice a day for 2 consecutive days. The present results showed that food-deprivation decreased leptin expression and increased NPY expression in the PVN of the hypothalamus. Food-deprivation decreased short-term memory ability and also suppressed BDNF expression in the hippocampus. However, Gambisu inhalation increased leptin expression and decreased NPY expression in the PVN of hypothalamus under the starvation conditions. Gambisu inhalation alleviated starvation-induced short-term memory impairment and enhanced BDNF expression in the hippocampus under the starvation conditions. The present study revealed that Gambisu inhalation might suppress desire for food intake and exert beneficial effect on cognition by enhancing BDNF expression in the hippocampus under the starvation conditions. (Korean J Str Res 2010;18:209∼217)

The Effect of Hyaluronic Acid on Full Thickness Defect of Articular Cartilage in Rabbit Knee

Kim, Yong Beum,Woo, Young Kyun CATHOLIC MEDICAL CENTER 1996 Bulletin of the Clinical Research Institute Vol.24 No.2

The hyaluronic acid, a high molecular weight glycoprotein ubiquitous in connective tissues, has protective functions as it plays role in the aggregation for proteoglycan, keeps synovial fluid viscoelasticity for lubrication, absorbs stress on cartilage, and protects articular cartilage surface layer. Recently scarless healing effect of hyaluronic acid on tissue has been also noted. The purpose of this study is to observe the hyaluronic acid effect on full thickness defected articular cartilage of rabbit kness. All experimental animals were subjected to operation to create full thickness defect at their distal femoral condyles. The experimental 48 animals were divided into 4 groups containing 12 animals in each. In group Ⅰ, 5mm diameter full thickness defect was created at their distal femoral condyle articular surface and left empty. In group Ⅱ, the same defect was created and grafted with autogenous perichondrium from the right costal cartilage. In group Ⅲ, the knee was injected with hyaluronic acid every week after creating same defect until the time of their sacrifice. In group Ⅳ, the same defect was created and grafted with perichondrium from the autogenous costal cartilage and hyaluronic acid was injected every week until the time of their sacrifice. Gross, histologic, histochemical, transmission electron microscopic and scanning electron microscopic observations were done for 12 weeks. The results were as follows; 1. Grossly at the 12th week, in group Ⅰ, the repaired tissue was rough and its margin was irregular but distinguishable from the adjacent normal cartilage. In group Ⅱ, the grafted tissue was well taken with even surface but margin was distinguishable from the adjacent normal cartilage. In group Ⅲ, the reparative tissue surface was regular and margin was smooth. In group Ⅳ, the grafted tissue was well integrated with smooth surface and its margin was undistinguishable from neighboring tissue. 2. Histologically, in group Ⅰ, fibrinoid arcade and red blood cells appeared in the defect site at the 4th week. The spindle shaped mesenchymal cell filled the cartilagenous defect as well as subchondral defect by the 8th week. At the 12th week, cartilagenous defect was filled mostly with fibroblast like cells. In group Ⅱ, grafted perichondria were integrated to surrounding tissue at the 8th week. And the defect was filled with tissue containing many chondrocytes at the 12th week. Group Ⅲ and Ⅳ showed almost complete healing of the articular cartilage by the 12th week. 3. Histochemical staining with safranin-O showed staining of matrix at the base of chondrocyte layer in group Ⅲ. In group Ⅳ, the whole layers of chondrocyte matrices were strongly stained. 4. Transmission electron microscope examination was carried out with 12 week samples. In group Ⅰ, the surface layer was irregular with disintegration of lamina splendes, and the arrangement of superficial and tangential layers of articular surface was also irregular. The underlying tissue contained spindle shape cells that resembled fibroblast. In group Ⅱ, the lamina splendes was absent, but underlying tissue contained normal appearing chondrocyte. In group Ⅲ and Ⅳ, preserved lamina splendes and regularly arranged collagen layer containing many chondrocytes were observed. And some chondrocytes at basal layer of repaired tissue exhibited well developed Golgi apparatus. 5. Scanning electron microscope was examined with tissue from the 12th week. In group Ⅰ, the regenerated tissue had rough surface with distinguishable margin, but it seemed well integrated. In group Ⅱ, the regenerated tissue was distinguished from the surrounding cartilage by a thin gap, and it had no lamina splendens with rough surface compared to normal articular cartilage. In group Ⅲ and Ⅳ, the margin was indistinguishable from normal cartilage and the surface was amooth and regular. These results suggest that the hyaluronic acid may play an important role in regenerating cartilage in full thickness defect of the rabbit knee joint.

A Study of Design of Hollow Fiber Membrane Modules for using in Artificial Lung by the PZT Actuator

Kim, Gi-Beum,Kim, Seong-Jong,Hong, Chul-Un,Lee, Yong-Chul,Kim, Min-Ho The Korean Society of Medical and Biological Engin 2006 의공학회지 Vol.27 No.4

The purpose of this work was to assess and quantify the beneficial effects of gas exchange, while testingto the various frequencies of the sinusoidal wave that was excited by the PZT actuator, for patients suffering from acute respiratory distress syndrome (ARDS) or chronic respiratory problems. Also, this paper considered a simulator to design a hollow type artificial lung, and a mathematical model was used to predict a behavior of blood. This simulation was carried out according to the Montecarno's simulation method, anda fourth order Runge-Kutta method was used to solve the equation. The experimental design and procedure are then applied to the construction of a new device to assess the effectiveness of the membrane vibrations. As a result, the vibration method is very effective in the increase of gas transport. The gas exchange efficiency for the vibrating intravascular lung assist device can be increased by emphasizing the following design features: consistent and reproducible fiber geometry, and most importantly, an active means of enhancing convective mixing of water around the hollow fiber membranes. The experimental results showed the effective performance of the vibrating intravascular lung assist device. Also, we concluded that important design parameters were blood flow rates, fiber outer diameter and oxygen pressure drop. Based on the present results, it was believed that the optimal level of blood flow rates was 200$cm^3$/min.

Preoperational Vibration Test of A Primary Heat Transport System of The CANDU Reactor

Yong Beum Kim,Seon Ho Song 한국소음진동공학회 1997 한국소음진동공학회 국제학술대회논문집 Vol.1997 No.1