Two New Species of Placolecis (Lichenized Ascomycota) from China

( An Cheng Yin ),( Xin Yu Wang ),( Dong Liu ),( Yan Yun Zhang ),( Mei Xia Yang ),( Li Juan Li ),( Li Song Wang ) 한국균학회 2019 Mycobiology Vol.47 No.4

Two new species of the lichen genus Placolecis are discovered in China, namely P. kunmingensis An. C. Yin & Li S. Wang and P. sublaevis An. C. Yin & Li S. Wang. The new combination P. loekoesiana (S.Y. Kondr., Farkas, J.J. Woo & Hur) An. C. Yin is proposed. Placolecis kunmingensis is characterized by having simple, spherical or ellipsoid, hyaline spores, and pear-shaped pycnidia; while P. sublaevis can be distinguished by its thallus forming larger aggregations with slightly flattened lobes at the thallus margin, and urn-shaped pycnidia. Descriptions, a phylogenetic tree and a key are provided for all the known Placolecis species in China.

The Einstein-K\"ahler metrics onHua domain

An Wang,Weiping Yin 대한수학회 2003 대한수학회지 Vol.40 No.4

In this paper we describe the Einstein-K"ahler metric for theCartan-Hartogs of the first type which is the special case of theHua domains. Firstly, we reduce the Monge-Amp` ere equation forthe metric to an ordinary differential equation in the auxiliaryfunction X=X(z,w)=|w|^2[det(I-ZZ^T)]^{-frac{1}{K}}(see below).This differentialequation can be solved to give an implicit function in X.Secondly, we get the estimate of the holomorphic section curvatureunder the complete Einstein-K"ahler metric on this domain.

N6-Methyladenosine modification (m6 A) of circRNA-ZNF638 contributes to the induced activation of SHF stem cells through miR-361-5p/Wnt5a axis in cashmere goats

Yin Ronghuan,Yin Ronglan,Bai Man,Fan Yixing,Wang Zeying,Zhu Yubo,Zhang Qi,Hui Taiyu,Shen Jincheng,Feng Siyu,Bai Wenlin 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4

Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6Adeficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHFstem cells. We further demonstrated that the internal m6A modification within circRNAZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNAZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis.

Assessment of Greenhouse Gas Emissions from Poultry Enteric Fermentation

Wang, Shu-Yin,Huang, Da-Ji Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.6

Emissions of nitrous oxide (N$_2$O) and methane (CH$_4$) from poultry enteric fermentation were investigated using a respiration chamber. Birds were placed in a respiration chamber for certain intervals during their growing period or for the whole life cycle. The accumulated gas inside the chamber was sampled and analyzed for N$_2$O and CH$_4$ production. A curve for gas production during a life cycle was fitted. The calculated area under the curve estimated the emission factor of poultry enteric fermentation on a life cycle basis (mg bird$^{-1}$ life cycle$^{-1}$). This method can be used to estimate CH$_4$ or N$_2$O emissions from different types of avian species taking into account factors such as diet, season or thermal effects. The CH$_4$/N$_2$O emission factors estimated for commercial broiler chickens, Taiwan country chickens and White Roman Geese were 15.87/0.03, 84.8/16.4 and 1,500/49 (mg bird$^{-1}$ life cycle$^{-1}$), respectively, while the calculated CH$_4$/N$_2$O emission from enteric fermentations were 3.03/0.006, 14.73/2.84 and 9.5/0.31 (Mg year$^{-1}$), respectively in Taiwan in the year of 2000. The described method is applicable to most poultry species and the reported emission factors were applicable to meat type poultry only.

Utility of DNA barcoding for identification of common Vespa species (Hymenoptera: Vespidae) from Yunnan, China

Wang Ming‐Ming,Yin Peng‐Kai,Tang Ya‐Ni,Yang Zi‐Zhong,Xiao Huai,Zhang Cheng‐Gui,Yang Yin‐He,Yang Da‐Song 한국곤충학회 2022 Entomological Research Vol.52 No.3

As traditional Chinese medicine and edible insects, Ve s p a hornets have high economic value and huge market demand. Hornet breeding has become an important way to increase the income of mountain farmers in China, but the limitation of traditional identification methods has become the bottleneck of the modern development of hornet resources. In this study, a simple and reliable method for the molecular identification of typical hornets from Yunnan was successfully established. In the NCBI database, DNA sequences of hornet samples were aligned as Vespa analis, Ve s p a b a s a l i s , Vespa velutina, Vespa mandarinia, Ve s p a m a g n i f i c a , Ve s p a b i n g h a m i , Ve s p a s o r o r by BLAST search. The efficiency of the PCR amplification of mitochondrial cytochrome C oxidase I (COI) gene sequences in all samples was 100% by using the optimized program. Moreover, a reference database for DNA barcodes of Vespa materials in Yunnan was constructed. This study provided a successful example for the identification of local insects of Yunnan and will be helpful to the development of wasp resources.

Stem-leaf saponins from Panax notoginseng counteract aberrant autophagy and apoptosis in hippocampal neurons of mice with cognitive impairment induced by sleep deprivation

Yin Cao,Yingbo Yang,Hui Wu,Yi Lu,Shuang Wu,Lulu Liu,Changhong Wang,Fei Huang,Hailian Shi,Beibei Zhang,Xiaojun Wu,Zhengtao Wang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioningat molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegenerationin the central nervous system. This study is aimed to explore the alleviative effect and theunderlying molecular mechanism of stemeleaf saponins of Panax notoginseng (SLSP) on the abnormalneuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory inducedby SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronalmorphological changes were observed by Nissl staining. Autophagosome formation was examined bytransmission electron microscopy, immunofluorescent staining, acridine orange staining, and transienttransfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin Vstaining. The expression or activation of autophagy and apoptosis-related proteins were detected byWestern blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h,accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampalneurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycinand re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transductionin HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probablythrough suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings maycontribute to the clinical application of SLSP in the prevention or therapy of neurological disordersassociated with SD.

Stochastic analysis for uncertain deformation of foundations in permafrost regions

Wang, Tao,Zhou, Guoqing,Wang, Jianzhou,Zhao, Xiaodong,Yin, Leijian Techno-Press 2018 Geomechanics & engineering Vol.14 No.6

For foundations in permafrost regions, the displacement characteristics are uncertain because of the randomness of temperature characteristics and mechanical parameters, which make the structural system have an unexpected deviation and unpredictability. It will affect the safety of design and construction. In this paper, we consider the randomness of temperature characteristics and mechanical parameters. A stochastic analysis model for the uncertain displacement characteristic of foundations is presented, and the stochastic coupling program is compiled by Matrix Laboratory (MATLAB) software. The stochastic displacement fields of an embankment in a permafrost region are obtained and analyzed by Neumann stochastic finite element method (NSFEM). The results provide a new way to predict the deformation characteristics of foundations in permafrost regions, and it shows that the stochastic temperature has a different influence on the stochastic lateral displacement and vertical displacement. Construction disturbance and climate warming lead to three different stages for the mean settlement of characteristic points. For the stochastic settlement characteristic, the standard deviation increases with time, which imply that the results of conventional deterministic analysis may be far from the true value. These results can improve our understanding of the stochastic deformation fields of embankments and provide a theoretical basis for engineering reliability analysis and design in permafrost regions.

Computerized bone age estimation system based on China-05 standard

Yin, Chuangao,Zhang, Miao,Wang, Chang,Lin, Huihui,Li, Gengwu,Zhu, Lichun,Fei, Weimin,Wang, Xiaoyu Techno-Press 2022 Advances in nano research Vol.12 No.2

The purpose of this study is to develop an automatic software system for bone age evaluation and to evaluate its accuracy in testing and feasibility in clinical practice. 20394 left-hand radiographs of healthy children (2-18 years old) were collected from China Skeletal Development Survey data of 1998 and China Skeletal Development Survey data of 2005. Three experienced radiologists and China-05 standard maker jointly evaluate the stages of bone development and the reference bone age was determined by consensus. 1020 from 20394 radiographs were picked randomly as test set and the remaining 19374 radiographs as training set and validation set. Accuracy of the automatic software system for bone age assessment is evaluated in test set and two clinical test sets. Compared with the reference standard, the automatic software system based on RUS-CHN for bone age assessment has a 0.04 years old mean difference, ±0.40 years old in 95% confidence interval by single reading, a 85.6% percentage agreement of ratings, a 93.7% bone age accuracy rate, 0.17 years old of MAD, 0.29 years old of RMS; Compared with the reference standard, the automatic software system based on TW3-C RUS has a 0.04 years old mean difference, a ±0.38 years old in 95% confidence interval by single reading, a 90.9% percentage agreement of ratings, a 93.2% bone age accuracy rate, a 0.16 years of MAD, and a 0.28 years of RMS. Automatic software system, AI-China-05 showed reliably accuracy in bone age estimation and steady determination in different clinical test sets.

Preparation of Selenium-enriched Bifidobacterium Longum and its Effect on Tumor Growth and Immune Function of Tumor-Bearing Mice

Yin, Yan,Wang, Rong-Rong,Wang, Yan,Wang, Jian-Jun,Xu, Gen-Xing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

In this study, we demonstrated selenium (Se) accumulation in Bifidobacterium longum strain (B. longum) and evaluated the effect of Se-enriched B. longum (Se-B. longum) on tumor growth and immune function in tumor-bearing mice. Analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) revealed that more than 99% of Se in Se-B. longum was organic, the main component of which was selenomethionine (SeMet). In the in vivo experiments, tumor-bearing mice (n=8) were orally administrated with different doses of Se-B. longum alone or combined with cyclophosphamide (CTX). The results showed that the middle and high dose of Se-B. longum significantly inhibited tumor growth. When Se-B. longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time of tumor-bearing mice (n=12) was prolonged. Furthermore, compared with CTX alone, the combination of Se-B. longum and CTX stimulated the activity of natural killer (NK) cells and T lymphocytes, increasing the levels of interleukin-2 (IL-2) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and the leukocyte count of H22 tumor-bearing mice (n=12).

Adsorption of Pb(II) from aqueous solution to Ni-doped bamboo charcoal

Yin Wang,Xuejiang Wang,Xin Wang,Mian Liu,Zhen Wu,Lianzhen Yang,Siqing Xia,Jianfu Zhao 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.1

Bamboo charcoal (BC) obtained by pyrolysis of Makino bamboo in the absence of oxygen was used as support for the preparation of Ni-doped adsorbent (Ni-BC). The low-cost composite was characterized and used as an adsorbent for Pb(II) removal from water. The results showed that both BET surface area and total pore volume of Ni-BC increased. The adsorption of Pb(II) strongly depended on solution pH,temperature and ionic strength. The adsorption isotherms followed Langmuir isotherm model well, and the maximum adsorption capacities of Pb(II) at 298 K were 25.0 and 142.7 mg/g for BC and Ni-BC,respectively. The adsorption processes were well fitted by pseudo-second-order kinetic model. Thermodynamic parameters showed that the adsorptions of Pb(II) onto both adsorbents were feasible,spontaneous, and exothermic under the studied conditions. The spent Ni-BC could be readily regenerated for reuse.