현직 교사의 일과 학업에의 균형에 대한 질적 사례연구 : 교육학과 대학원생을 중심으로

류루루(Liu, LuLu),이지인(Lee, Jiin),마루오시(Ma, RuoXi),서영주(Seo, Youngjoo) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.4

목적 본 연구는 대학원에 재학중인 현직 교사의 대학원 생활의 경험을 통해 그들의 일과 학업에 대해 겪은 어려움과 해결법을 파악하고, 현직 교사에 대한 재교육 과정의 시사점을 도출하는 데 그 목적이 있다. 방법 본 연구의 목적을 달성하기 위하여 내러티브 탐구를 활용하였다. 연구 참여자는 국내 A대학의 대학원에 재학중인 현직 교사 6명을 유의적 표집한 후, 반구조화 면담으로 수집된 현장 텍스트를 통해 참여자의 일과 학업의 균형 경험에 관한 이야기를 중심으로 내러티브 시간적 차원에 근거하여 분석하였다. 연구의 타당성을 확보를 위해서는 대화와 협의를 통해 의미를 분석하고 상호 확인하는 과정을 거침으로써 연구자가 주관적인 오류에 빠지지 않도록 주의를 기울였다. 결과 재학중인 현직 교사들의 대학원 진학 동기는 학문에 대한 열정, 진로, 생활에 대한 도움 등으로 나타났다. 그들이 대학원생활 적응 초기 경험하는 어려움은 주로 난이도가 높은 학업과 쓰기가 어려움 논문으로 나타났으며, 일과 학업의 불균형으로 인한 건강상태의 악화, 여가 시간 부족, 직장 생활에 부정적인 영향 등 어려움을 겪고 있었다. 이로 인한 갈등이 생기고 스트레스가 쌓이지만 가족과 친구의 지지, 교수와 직장의 이해와 배려가 일부 교사에게 견디는 힘이 되었다. 그럼에도 불구하고, 일과 학업의 불균형 문제를 해결하기어려움으로 인해 이런 문제를 해결할 수 없는 일부 교사가 학업 포기하든지 휴직을 선택하기도 한다. 결론 대학원에 재학중인 현직 교사가 일과 학업 간의 균형을 잘 잡을 수 있도록 정부와 학교가 같이 협력하여 도움을 줄 수 있는 적절한 교육 방안 마련이 필요하다. Objectives The purpose of this study were to identify the difficulties and solutions experienced in their work and study through the graduate life experiences of in-service teachers attending a graduate school, and offer insights to the Korean universities deriving implications for the re-training process of them. Methods This study explores work-study balance experiences of six in-service teachers attending “A” university graduate school in Busan City, Korea. Using narrative inquiry to elicit their stories in their own voices, the field texts collected from semi-structured interviews were analyzed focusing on critical experiences throughout their work-study balance. Results The results indicate that in-service teachers motivation for entering graduate school was found to be passion for study, career, and help with life. The difficulties they experience in adapting to graduate life were mainly found to be high-level academic and difficult-to-write papers at the beginning. Work-study imbalance caused them to have a lot of life problems, such as poor health, lack of leisure time, negative effects on work life, and this problems lead to conflicts and stress of life. The support of family and friends, understanding and consideration of professors and workplaces, it was a force to hold on to some in-service teachers. However, it is difficult to solve the problem even if the teacher makes his or her own efforts while receiving a lot of external support. Therefore, some in-service teacher who cannot solve these problems may give up their studies or work. Conclusions It is necessary to prepare an appropriate educational plan for the government and schools to cooperate and help in-service teachers attending graduate school so that they can balance work and study well.

Strength of connection fixed by TOBs considering out-of-plane tube wall deformation-Part 1: Tests and numerical studies

Tuoya Wulan,Peijun Wang,Chengxin Xia,Xinyu Liu,Mei Liu,Fangzhou Liu,Ou Zhao,Lulu Zhang 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.1

This paper presents a study on the behavior of a bolted T-stub to square tube connection using Thread-fixed Oneside Bolts (TOBs) through tests and numerical simulations. It outlines a research work of four connections with focus on the failure modes and strengths of the connection under tensile load. It was observed that the thread anchor failure caused by shear failure of hole threads controlled the final failure of the connection in the tests. Meanwhile, the out-of-plane deformation of tube wall resulted in the contact separation between hole threads and bolt threads, which in turn reduced the shear strength of hole threads. Finite element models (FEMs) allowing for the configuration details of the TOBs fixed connection are then developed and compared with the test results. Subsequently, the failure mechanism of hole threads and stress distribution of each component are analyzed based on FEM results. It was concluded that the ultimate strength of connection was not only concerned with the shear strength of hole threads, but also was influenced by the plastic out-of-plane deformation of tube wall. These studies lay a foundation for the establishment of suitable design methods of this type of connection.

Cytosolic 5′-Methylthioadenosine/ S-Adenosylhomocysteine Nucleosidase MtaN from Mycobacterium. smegmatis: purification, crystallization and X-ray crystallographic analysis

Lulu Wang,Yuanyuan Chen,Wei Liu,Jing Lan,Fei Shang,Nam-Chul Ha,Yuesheng Dong,Chunshan Quan,Yongbin Xu 한국구조생물학회 2019 Biodesign Vol.7 No.3

Mycobacterium tuberculosis is a dangerous pathogen, and it can cause the most deadly disease tuberculosis (TB). Nonpathogenic Mycobacterium smegmatis is an important model for studying the M. tuberculosis. M. smegmatis 5’-Methylthioadenosine/S-adenosyl-L-homocysteine nucleosidases (MtaNs) catalyze the hydrolysis of adenine from 5’-methylthioadenosine (MTA), MtaNs cleave the glycosidic bond of MTA or S-adenosylhomocysteine (SAH) irreversibly. In this study, MtaN from M. smegmatis (MsMtaN) was successfully expressed and purified using Ni-NTA affinity, Q anionexchange and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.0 Å. The crystal belonged to the orthorhombic space group P1211, with unit-cell parameters of a =57.6, b = 172.6, and c = 183.3 Å. The Matthews coefficient and solvent content were estimated to be 2.32 Å3 Da-1 and 47%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule. Size-exclusion chromatography suggested that MsMtaN prefer to exist as tetramer in solution.

Effects of surface materials of self-draining beds on cattle behavior in a temperate climate

Liu Ping,Guo Lulu,Zhang Fulan,Li Lin,Mao Huaming,Gu Zhaobing 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: The objective of the present experiment was to construct self-draining beds to keep surface bedding materials clean and dry for beef cattle comfort in a temperate climate. Methods: In Experiment 1, a self-draining bed was covered with sand at depths of 10 cm (S-10a), 15 cm (S-15), and 20 cm (S-20) respectively. In Experiment 2, self-draining beds of different sizes were covered with 10 cm of sand (S-10b) and wood shavings (WS) at depths of 15 cm and 20 cm (WS-15 and WS-20). Fifteen cattle were engaged to evaluate the comfort of self-draining beds covered with different bedding materials. Results: No cattle lay in the feed alley and cattle spent more time lying on S-10a than S-15 or S-20 in Experiment 1 (p<0.01). No difference in lying time was detected between S-15 and S-20 (p>0.05). In Experiment 2, no cattle selected the feed alley as the lying area. Cattle preferred WS-15 as the lying area and time spent lying on WS-20 was slightly higher than on S-10b (p<0.05). Feces weight was higher in the feed alley than in the different bedding areas in both Experiments 1 and 2 (p<0.01). Conclusion: Sand-bedding depth at 10 cm and WSs at 15 cm above the self-draining bed can provide for the lying comfort of beef cattle. Design of a special feed alley to hold most of the feces to keep bedding materials clean and dry is desirable for organic beef cattle in a loose barn.

Fertility-preserving treatment outcome in endometrial cancer or atypical hyperplasia patients with polycystic ovary syndrome

Lulu Wang,Xuezhen Luo,Qian Wang,Qiaoying Lv,Pengfei Wu,Wei Liu,Xiaojun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.5

Objective: This study aimed to investigate the impact of polycystic ovary syndrome (PCOS) on fertility-sparing treatment in young patients with atypical endometrial hyperplasia (AEH) or endometrioid endometrial cancer (EEC). Methods: A total of 285 patients with EEC (n=76, FIGO stage IA, without myometrium invasion) or AEH (n=209) who received progestin-based fertility-sparing treatment were evaluated retrospectively. Among the 285 patients, 103 (36.1%), including 70 AEH cases and 33 EEC cases, were diagnosed with PCOS. General characteristics, cumulative 16- and 32-week complete response (CR) rate, pregnancy outcome and recurrence were compared between patients with or without PCOS. Results: The cumulative 16-week CR rate was lower in the PCOS group than in the non- PCOS group (18.4% vs. 33.8%, p=0.006). Patients with PCOS took longer treatment duration to achieve CR (7.0 months vs. 5.4 months, p=0.006) and shorter time to relapse after CR (9.6 months vs. 17.6 months, p=0.040) compared with non-PCOS group. After adjusting for patient age, body mass index, PCOS, homeostasis model assessment-insulin resistance index, and serum testosterone levels, we found that body mass index ≥25 kg/m2 (HR=0.583; 95% CI=0.365–0.932; p=0.024) and PCOS (HR=0.545; 95% CI=0.324–0.917; p=0.022) were significantly correlated with lower 16-week CR rate. Conclusion: PCOS was associated with lower 16-week CR rate, longer treatment duration and shorter recurrence interval in patients with AEH or EEC receiving fertility-preserving treatment.

Structural insight into the carboxylesterase BioH from <i>Klebsiella pneumoniae</i>

Wang, Lulu,Chen, Yuanyuan,Shang, Fei,Liu, Wei,Lan, Jing,Gao, Peng,Ha, Nam-Chul,Nam, Ki Hyun,Dong, Yuesheng,Quan, Chunshan,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.520 No.3

<P><B>Abstract</B></P> <P>The BioH carboxylesterase which is a typical α/β-hydrolase enzyme involved in biotin synthetic pathway in most bacteria. BioH acts as a gatekeeper and blocks the further elongation of its substrate. In the pathogen <I>Klebsiella pneumoniae</I>, BioH plays a critical role in the biosynthesis of biotin. To better understand the molecular function of BioH, we determined the crystal structure of BioH from <I>K. pneumoniae</I> at 2.26 Å resolution using X-ray crystallography. The structure of KpBioH consists of an α-β-α sandwich domain and a cap domain. B-factor analysis revealed that the α-β-α sandwich domain is a rigid structure, while the loops in the cap domain shows the structural flexibility. The active site of KpBioH contains the catalytic triad (Ser82-Asp207-His235) on the interface of the α-β-α sandwich domain, which is surrounded by the cap domain. Size exclusion chromatography shows that KpBioH prefers the monomeric state in solution, whereas two-fold symmetric dimeric formation of KpBioH was observed in the asymmetric unit, the conserved Cys31-based disulfide bonds can maintain the irreversible dimeric formation of KpBioH. Our study provides important structural insight for understanding the molecular mechanisms of KpBioH and its homologous proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Crystal structure of <I>K. pneumoniae</I>BioH consists of an α-β-αsandwish domain and acapsubdomain. </LI> <LI> The active site of KpBioH contains the catalytic triad (Ser82-Asp207-His235). </LI> <LI> The Arg138, Arg142, Arg155, and Arg159 of KpBioH may play an important role in the binding of the substrates like ACP. </LI> <LI> The Cys31-based disulfide bonds can maintain the irreversible dimeric formation of KpBioH. </LI> </UL> </P>

Distribution of nitrate and its implication for the contaminant source in groundwater of Huaibei Plain, Anhui Province

Jiazhong Qian,Lulu Wang,Yong Liu,Boren Wu,Xiaoming Wang 한국지질과학협의회 2015 Geosciences Journal Vol.19 No.3

High concentration of NO3-N in groundwater can pose risk to human’s health, thus the study of NO3-N contamination in groundwater is a very important issue related to public health. Using a typical agriculture zone, Huaibei plain in Anhui Province of China as a field site, the temporal and spatial variation of groundwater NO3-N concentration was studied using a multivariate statistical factor analysis combined with Kaiser-Meyer- Olkin method based on a hydrogeological investigation from 2005 to 2009 year. The results show: (1) The contaminant in the groundwater of Huaibei Plain in Anhui Province are serious. 10% of the shallow groundwater samples in non-rainy season of 2005 exceeds the recommended value of NO3-N by Chinese drinking water standard (≤20 mg/L), while 17.5% exceeds the recommended value (11.3 mg/L) by EC. (2) About 15% of shallow groundwater in the whole study area was polluted in 2005 with the maximum of 97.67 mg/L; Groundwater NO3-N concentration is higher in non-rainy season than in rainy season. And the NO3-N content in groundwater shows the increasing trend at all three depths sampled from 2005 to 2009 year. (3) Groundwater nitrate pollution mainly derives from anthropogenic activities in Huaibei Plain, Anhui Province. Further quantitative analysis need to be studied in the future.

One-Pot Synthesis of Nanodendritic PtIr Alloy with High Electrochemical Activity for Ethylene Glycol Oxidation

Xuedong Gao,Lulu Liu,Qiyu Wang,Kun Qi,Zhao Jin,WEITAO ZHENG,XIAOQIANG CUI 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.2

Nanodendritic Pt-based bimetallic alloys are one promising catalyst with three-dimensional (3D) networks structure composed of integrating branches for electrochemical catalytic reaction. We successfully synthesized dendrites Pt6Ir4 alloy with small size of 20 nm in oleylamine. The dendritic Pt6Ir4 alloy are characterized by high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The electrochemical tests suggest that the as-prepared dendritic Pt6Ir4 alloy exhibits greatly enhanced ethylene glycol oxidation reaction (EGOR) activity than commercial Pt/C with high EGOR mass activity, anti-poisoning and stability.

Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs

Shaohua Yang,Lulu Wang,Ying Wang,Xiaoqian Ou,Zhaoyuan Shi,Chongchong Lu,Wei Wang,Guoqing Liu 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.5

Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1- diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (•OH), superoxide anion (O2-•) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity. Based on this, the antioxidant protein of the samples on day 16 were isolated for the follow-up study. With a molecular weight 43.22 kDa, the antioxidant protein was purified by Diethylaminoethyl cellulose -52 (DEAE-52) column and Sephadex G-100. The LC-MS analysis showed that the purified protein molecular weight was 43.22 kDa, named D2-S. The sequence of amino acids was highly similar to ovalbumin and the coverage reached to 84%. The purified protein showed a radical scavenging rate of 52.34±3.27% on DPPH and 63.49±0.25% on •OH, respectively. Furthermore, the C-terminal amino acid sequence was NAVLFFGRCVSP, which was consistent with the sequence of ovabumin. These results here indicated that purified protein may be a potential resource as a natural antioxidant.

Preparation of magnetic carbon spheres derived form 8-quinoliolato Fe (III) complexe and its application in water treatment

Zhigang Jia,Lulu Yang,Jianhong Liu,Qiuze Wang,Rongsun Zhu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

Magnetic carbon spheres have been successfully prepared by the synchronous carbonation and activationof the 8-quinoliolato Fe(III) complexe with sphere morphology, which has been synthesized bysolovthermal method. The obtained composite possesses relatively even pore diameter of 3.8 nm, highsurface area of 335.59m2/g with 52.67 wt.% Fe3O4 coated, and a saturation magnetization (Ms) of35.27 emu/g. Dye separationperformance of the magnetic carbon spheres is investigated using methyleneorange and methylene blue as modle. The resluts show that the maxium adsorption capacity formethylene orange and methylene blue is 128.70mgg 1 and 151.29mgg 1, respectively, and the processcan rapidly reach adsorption equilibrium within 5 min, which is important for the application of the aspreparedmagnetic carbon spheres.