Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)

양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.

Continuous perfusion culture for generation of functional tissue-engineered soft tissues

Y.I. Yang,D.L. Seol,H.I. Kim,M.H. Cho,S.J. Lee 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

Culture conditions for generation of functional engineered tissues are designed to perform at least one of the following functions: (1)provide ecient mass transfer to the growing tissues. In this study, we have investigated the eectiveness of a perfusion culture system forconstruction of tissue-engineered soft tissues (TESTs). TESTs were constructed on poly(D,L-lactide-co-glycolic acid) (PLGA) scaoldswith HDFs. We compared biological and mechanical properties of TESTs constructed by static and perfusion culture conditions.The TESTs generated by perfusion culture revealed signicantly higher DNA (p < 0.01), GAG content (p < 0.01), and elastic modulus(p < 0.05) than TESTs constructed by static culture. Moreover, homogeneous distribution of HDFs and ECM throughout the scaoldwas noted in TESTS constructed by perfusion culture, rather than spatial variation, which was observed in TESTs constructed by staticculture. In an in vivo study, increased tissue regeneration was observed in TESTs fabricated by perfusion culture. Therefore, it is sug-gested that the perfusion culture system can constitute a more promising approach for constructing functional TESTs.

Effects of Immune Stress on Performance Parameters, Intestinal Enzyme Activity and mRNA Expression of Intestinal Transporters in Broiler Chickens

Feng, Y.,Yang, X.J.,Wang, Y.B.,Li, W.L.,Liu, Y.,Yin, R.Q.,Yao, J.H. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.5

Immune stress is the loss of immune homeostasis caused by external forces. The purpose of this experiment was to investigate the effects of immune stress on the growth performance, small intestinal enzymes and peristalsis rate, and mRNA expression of nutrient transporters in broiler chickens. Four hundred and thirty-two 1-d-old broilers (Cobb500) were randomly assigned to four groups for treatment; each group included nine cages with 12 birds per cage. Group 1 = no vaccine (NV); Group 2 = conventional vaccine (CV); group 3 = lipopolysaccharide (LPS)+conventional vaccine (LPS); group 4 = cyclophosphamide (CYP)+conventional vaccine (CYP). The results demonstrated that immune stress by LPS and CYP reduced body weight gain (BWG), feed intake (FI), small intestine peristalsis rate and sIgA content in small intestinal digesta (p<0.05). However, the feed conversion ratio (FCR) remained unchanged during the feeding period. LPS and CYP increased intestinal enzyme activity, relative expression of SGLT-1, CaBP-D28k and L-FABP mRNAs (p<0.05). LPS and CYP injection had a negative effect on the growth performance of healthy broiler chickens. The present study demonstrated that NV and CV could improve growth performance while enzyme activity in small intestine and relative expression of nutrient transporter mRNA of NV and CV were decreased in the conditions of a controlled rational feeding environment. It is generally recommended that broilers only need to be vaccinated for the diseases to which they might be exposed.

Epitaxial Multiferroic BiFeO3 Thin Films: Progress and Future Directions

Chu, Y. H.,Martin, L. W.,Zhan, Q.,Yang, P. L.,Cruz, M. P.,Lee, K.,Barry, M.,Yang, S. Y.,Ramesh, R. Taylor Francis 2007 Ferroelectrics Vol.354 No.1

<P> We write this article in honor of Professor Vitaly L. Ginzburg, truly the father of the field of ferroelectricity. This article serves as a review of the current state of research pertaining to multiferroic BiFeO3 thin films. In this review we will delve into details of the growth of BiFeO3 thin films and the use of piezoforce microscopy and x-ray reciprocal space mapping to characterize the crystal structure and domain structure of BiFeO3. We will also discuss the use of vicinal and asymmetric substrates to simplify the domain structure in BiFeO3. By simplifying the domain structure we can, in turn, control the ferroelectric switching mechanisms in BiFeO3. Finally we describe the basic ferroelectric properties of BFO films and discuss the critical issues needed to be solved in BiFeO3 films including leakage, complex domain structure, coercivity, and reliability. Such results are promising for continued exploration for detailed multiferroic-coupling studies in the magnetoelectric BiFeO3 system and BiFeO3, in turn, provides a model platform with which to realize the exciting possibility of electrically control magnetism.</P>

Biogenic reductive preparation of magnetic inverse spinel iron oxide nanoparticles for the adsorption removal of heavy metals

Lingamdinne, L.P.,Chang, Y.Y.,Yang, J.K.,Singh, J.,Choi, E.H.,Shiratani, M.,Koduru, J.R.,Attri, P. Elsevier 2017 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.307 No.-

The accumulation of heavy metal ions in living cells impairs the organ function of living creatures. So, needs to develop new materials with high efficiency of heavy metals removal from the environment. As part of this effort, here, synthesized magnetic inverse spinel iron oxide nanoparticles (MISFNPs) using a biogenic methodology. To synthesize the biogenic MISFNPs, we used the seed extract of Cnidiummonnieri (L.) Cuss (CLC) as a precursor. The prepared MISFNPs was characterized using PXRD, FT-IR, SEM-EDX, SEM, BET, AFM and XPS. We also investigated the size, surface area, structure and magnetic properties of MISFNPs. Additionally, magnetic property measurement system (MPMS) studies show that our prepared MISFNPs was superparamagnetic at room temperature. Further, we used the MISFNPs for the removal of Pb(II) and Cr(III) from aqueous solutions through batch studies. Batch adsorption studies revealed that Pb(II) and Cr(III) follow pseudo-second-order kinetics during adsorption onto the homogenous surface of MISFNPs. Besides, we also found that the adsorption of Pb(II) and Cr(III) on nanoparticles followed an endothermic process. Lastly, we concluded that MISFNPs synthesized by a green route is capable of recycling and removal of heavy metals without loss of its stability.

Inhibition of tyrosinase by fumaric acid: Integration of inhibition kinetics with computational docking simulations

Gou, L.,Lee, J.,Yang, J.M.,Park, Y.D.,Zhou, H.M.,Zhan, Y.,Lu, Z.R. Elsevier 2017 International Journal of Biological Macromolecules Vol.105 No.3

Fumaric acid (FA), which is naturally found in organisms, is a well known intermediate of the citric acid cycle. We evaluated the effects of FA on tyrosinase activity and structure via enzyme kinetics and computational simulations. FA was found to be a reversible inhibitor of tyrosinase and its induced mechanism was the parabolic non-competitive inhibition type with IC<SUB>50</SUB>=13.7+/-0.25mM and K<SUB>islope</SUB>=12.64+/-0.75mM. We newly established the equation for the dissociation constant (K<SUB>islope</SUB>) for the parabolic inhibition type in this study. Kinetic measurements and spectrofluorimetry studies showed that FA induced regional changes in the active site of tyrosinase. One possible binding site for FA was identified under the condition without L-DOPA. The computational docking simulations further revealed that FA can interact with HIS263 and HIS85 at the active site. Furthermore, four important hydrogen bonds were found to be involved with the docking of FA on tyrosinase. Our study provides insight into the mechanism by which dicarboxylic acids such as FA inhibit tyrosinase. By inhibiting tyrosinase and its central role in pigment production, FA is a potential natural antipigmentation agent.

스마트폰을 이용하는 노인의 우울, 고독감과 사회적 지지에 대한 연구

이세미,장혜원,정여주,김여울,정지혜,김지원,김주영,양혜린,박지혜,정유리 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49

Purpose: The purpose of this study was to investigate depression, loneliness, and social support status of the elderly using smartphone. Method: In this descriptive design study, 100 elderly people using smartphone were recruited through convenient sampling between August and September, 2014. Questionnaires used for this study were CES-D(The Center for Epidemiological Studies-Depression Scale), UCLA Loneliness Scale, and social support scale developed by Park. Data were statistically analyzed using SPSS 22.0 program with descriptive statistics, independent t-test, one way ANOVA with Scheffe's test, and χ2-test. Result: Smartphone usage duration had significant influence on depression(=0.000) and loneliness(=0.001) of the elderly using smartphone. In detail, depression scores of participants using smartphone less than 6 months were higher than participants of more than 2 years(p=0.004). And both depression and loneliness scores of participants using smartphone 6 to 1 year were higher than participants of more than 2 years(=0.001). But smartphone usage time had no significant influence on depression and loneliness of the elderly using smartphone. Conclusion: The result of this study suggest that smartphone usage duration is significantly related to both depression and loneliness of the elderly. Therefore, smartphone education program for the elderly would be a helpful intervention for depression and loneliness of the elderly. And further studies are required with more sample size and more systematic data collecting methods to confirm these results.

20대여성의 유방자가검진에 대한 지식과 자기효능감의 관계연구

강지혜,유리나,박민아,박신영,양은주,이주은,전시은,정윤혜,최연아,홍재하,이자형,정덕유,배노연 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

The purpose of this study was to know relationship between important variables which influence lifestyle in college women`s health promotion, prove adjustable factors and provide basic data which develops nursing intervention health promotion program. The method of this study was descriptive correlational study. The convenience sample was 206 college woman who live alone in lodgings, dormitory, a reading room or off-campus housing. Research instruments were the Multidimensional Health Locus of Control(MHLC) developed by Wallston, Wallston & Devellis(1978), Interpersonal Support Evaluation List developed by Cohern, Hoberman(1983) and The Health Promotion Lifestyle Profile(HPLP) developed by Walker, Pender & Sechrist(1978) and the data was used after Factor Analysis. The result of this study was follows. 1. It can be found that the level of the college woman`s health promotion lifestyle was 2.46±.33 with higher fulfilling level: According to general characteristic, the level of health promotion lifestyle was 5% significant statistical differences by age and period of menstruation. 2. The level of health locus of control was 3.25±.31 with higher fulfilling level. 3. The interpersonal support level was 3.05±.44 with higher fulfilling level. 4. The level of interpersonal support turned out most convincing factor in health promotion lifestyle (9%). In addition to age factor, two factors was 10% power of explanation in whole health promotion.

여대생의 먹방 시청과 생활 스트레스, 음식중독 증상에 대한 연구

김대희,김영서,박채연,백정희,윤예원,장정원,전혜린,차윤정,Jiang Han,양숙자 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: This study aimed to know whether there is a comparison of food video watching, life stress, food addiction symptom for female college students. Methods: A quantitative research is used. A total of 162 people were performed with questionnaire which was conducted from September 16, 2019 to October 2, 2019. The study sample was female college students of one university in Seoul. This study used general characteristics, life stress tool, and food addiction symptoms tool as a research tool. In dependent T-test, ANOVA, and pearson’s correlation was used for this study. Result: The food video watching time and the food addiction symptoms showed a significant positive of correlation (r=.230, p=.003). Also, life stress and food addiction symptoms were significantly correlated (r=.329, p<.001). However, food video watching time and life stress did not show statistically significant correlation (r=.062, p=.430). Conclusion: Based on the study results, food video watching time and life stress are highly correlated with food addiction symptoms. The findings of this study could be the basis for later research on nursing intervention in the life stress and food addiction symptoms among female college students.

작은빨간집모기(Culex tritaeniorhynchus)의 개체군 밀도와 일본뇌염환자 발생 예찰

심재철,윤영희,조양벽,이주연,신이현,양영철,박정은,이욱교,김정림,이원자 국립보건연구원 1994 국립보건원보 Vol.31 No.1