비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

Blockade of RAGE ameliorates elastase-induced emphysema development and progression <i>via</i> RAGE-DAMP signaling

Lee, Hanbyeol,Park, Jeong-Ran,Kim, Woo Jin,Sundar, Isaac K.,Rahman, Irfan,Park, Sung-Min,Yang, Se-Ran The Federation of American Societies for Experimen 2017 The FASEB Journal Vol.31 No.5

<P>The receptor for advanced glycan end products (RAGE) has been identified as a susceptibility gene for chronic obstructive pulmonary disease (COPD) in genome-wide association studies (GWASs). However, less is known about how RAGE is involved in the pathogenesis of COPD. To determine the molecular mechanism by which RAGE influences COPD in experimental COPD models, we investigated the efficacy of the RAGE-specific antagonist FPS-ZM1 administration in in vivo and in vitro COPD models. We injected elastase intratracheally and the RAGE antagonist FPS-ZM1 in mice, and the infiltrated inflammatory cells and cytokines were assessed by ELISA. Cellular expression of RAGE was determined in protein, serum, and bronchoalveolar lavage fluid of mice and lungs and serum of human donors and patients with COPD. Downstream damage-associated molecular pattern (DAMP) pathway activation in vivo and in vitro and in patients with COPD was assessed by immunofluorescence staining, Western blot analysis, and ELISA. The expression of membrane RAGE in initiating the inflammatory response and of soluble RAGE acting as a decoy were associated with up-regulation of the DAMP-related signaling pathway via Nrf2. FPS-ZM1 administration significantly reversed emphysema in the lung of mice. Moreover, FPS-ZM1 treatment significantly reduced lung inflammation in Nrf2(+/+) , but not in Nrf2(-/-) mice. Thus, our data indicate for the first time that RAGE inhibition has an essential protective role in COPD. Our observation of RAGE inhibition provided novel insight into its potential as a therapeutic target in emphysema/COPD.-Lee, H., Park, J.-R., Kim, W. J., Sundar, I. K., Rahman, I., Park, S.-M., Yang. S.-R. Blockade of RAGE ameliorates elastase-induced emphysema development and progression via RAGE-DAMP signaling.</P>

단어출현빈도분석과 AHP를 통한 기술 연구 동향과 기대 효과 분석: 바이오 에너지 분리막을 중심으로

이인우(Lee, In-Woo),서한결(Seo, Han-Gyeol),장수진(Jang, Soo-Jeen),조철희(Cho, Churl-Hee),정양헌(Chung, Yang-Hon) 한국경영교육학회 2020 경영교육연구 Vol.35 No.5

[연구목적] 본 연구는 과학 기술 연구 동향과 기술 도입 기대 효과를 함께 분석하는 방안을 바이오 에너지 분리막 분야에 적용하여 연구개발 전략 제시에 도움이 되는 연구 방안을 제시하고자 하였다. [연구방법] 본 연구는 분리막 기술 연구 방향과 기대효과를 분석하기 위해 각각에 대해 두 가지 연구방법을 이용하였다. 우선 텍스트 마이닝을 통해 단어출현 빈도분석으로 선행연구의 연구 방향을 확인하였고, AHP를 통해 기대효과를 알아보았다. [연구결과] 분석결과 분리막 기술의 연구 방향은 주로 분리막 관련 다양한 공정과 공정개선을 통한 성능 향상에 있는 것으로 확인되었다. 분리막 기술을 통한 기대 효과는 바이오 에너지 공정에서 발생하는 환경 문제 해결이 주된 것으로 나타났다. [연구의 시사점] 분리막 관련 연구는 분리막 성능에 중점을 두고 진행되고 있으며 분리막을 실제 공정에 도입하는 측에서 기대하는 부분은 분리막 도입으로 인한 효과와 분리성능 향상에 의한 효과에 중점을 두고 있음을 확인하였다. 이는 분리막 기술 연구가 실제 니즈를 잘 반영하여 이루어지고 있음을 보여준다. 이처럼 특정 기술의 연구 방향과 도입에 따른 기대효과를 함께 분석할 수 있으며, 이는 연구개발 전략 제시에 도움이 될 수 있음을 확인하였다. [Purpose] Purpose of this study is to propose a research methodology that is helpful in presenting the R&D strategy by applying analysis on research trends and expected effect of technology introduction together in the field of bioenergy membranes. [Methodology] For this purpose, we used two research methodologies. Word frequency analysis of text mining was used to analyze the research trends of previous studies, and the expected effect was investigated through AHP. [Findings] This study found that the research trends of membrane is mainly to improve performance through various processes and expected effect focus on environmental problem solving arising from bioenergy process. [Implications] It was confirmed that main research trends of membrane is performance of membrane and expected effect is performance improvement by introduction of membrane in bioenergy processes. This shows that research on membrane is well reflected in actual needs. This methodology can be helpful in presenting the consumer-oriented R&D strategy.

제대군인 취업지원 프로그램 구안

정철영,서우석,김성호,이예성,최동선,이종범,이성식,이건남,양안나,김성남 한국직업능력개발원 2007 직업능력개발연구 Vol.10 No.1

이 연구의 목적은 중·장기복무를 마치고 제대하여 재취업하고자 하는 제대군인들의 원활한 전직을 돕기 위한 제대군인 취업지원 프로그램의 개발방향과 개발안을 제시하는 데 있다. 이를 위해 국내·외에서 이루어지고 있는 제대군인 취업지원 프로그램의 실태를 분석하였고, 제대군인 취업지원 프로그램에 대한제대예정군인 및 제대군인과 기업체의 요구를 분석하여 시사점을 도출하였다. 그리고 이를 기초로 제대군인 취업지원 프로그램의 기본방향과 전체 흐름도, 세부 프로그램 개발안을 제시하였다. 이를 위해서 활용된 연구의 방법은 문헌연구, 조사연구, 전문가협의회 등이다. 이 연구를 통해서 제시된 제대군인 취업지원 프로그램의 개발방향과 개발안을 토대로, 일관되고 지속적인 수요자 중심의 제대군인 취업지원 프로그램의 개발이 가능할 것이다. The purpose of this study is to suggest plans and directions for developing. job-seeking support program for military veterans. The study is conducted through literature review, survey and interview. The results of this study are as follows: ⒧ basic development directions and flowchart of job-seeking support program for military veterans are suggested; and ⑵ development plans for sub-programs are suggested.

BEZ235 (PIK3/mTOR inhibitor) Overcomes Pazopanib Resistance in Patient-Derived Refractory Soft Tissue Sarcoma Cells

Kim, Hee Kyung,Kim, Sun Young,Lee, Su Jin,Kang, Mihyeon,Kim, Seung Tae,Jang, Jiryeon,Rath, Oliver,Schueler, Julia,Lee, Dong Woo,Park, Woong Yang,Kim, Sung Joo,Park, Se Hoon,Lee, Jeeyun Neoplasia Press 2016 Translational oncology Vol.9 No.3

<P><I>BACKGROUND:</I> Although pazopanib treatment has become the standard chemotherapy in salvage setting for metastatic sarcoma patients, most patients progress after pazopanib treatment in 4 to 6 months. After failure to pazopanib, patients have limited options for treatment. Therefore, subsequent therapy in patients who failed to pazopanib is urgently needed and the use of patient derived cells or patient derived tumors for accompanying testing with various pharmacological inhibitors could offer additional treatment options for these patients. <I>METHODS:</I> Patient derived tumor cells were collected from ascites at the time of progression to pazopanib and a 13-drug panel was tested for drug sensitivity. We confirmed the results using <I>in vitro</I> cell viability assay and immunoblot assay. We also performed the genomic profiling of PDX model. <I>RESULTS:</I> The growth of patient derived tumor cells was significantly reduced by exposure to 1.0 μM AZD2014 compared with control (control versus AZD2014, mean growth = 100.0% vs 16.04%, difference = 83.96%, 95% CI = 70.01% to 97.92%, <I>P</I> = .0435). Similarly, 1.0 μM BEZ235 profoundly inhibited tumor cell growth <I>in vitro</I> when compared to control (control versus BEZ235, mean growth = 100.0% vs 7.308%, difference = 92.69%, 95% CI = 78.87% to 106.5%, <I>P</I> < .0001). Despite the presence of CDK4 amplification in the patient-derived tumor cells, LEE011 did not considerably inhibit cell proliferation when compared with control (control vs LEE011, mean growth = 100.0% vs 80.23%, difference = 19.77%, 95% CI = 1.828% to 37.72%, <I>P</I> = .0377). The immunoblot analysis showed that BEZ235 treatment decreased pAKT, pmTOR and pERK whereas AZD2014 decreased only pmTOR. <I>CONCLUSION:</I> Taken together, upregulation of mTOR/AKT pathway in sarcoma patient derived cells was considerably inhibited by the treatment of AZD2014 and BEZ235 with downregulation of AKT pathway (greater extent for BEZ235). These molecules may be considered as treatment option in STS patient who have failed to pazopanib in the context of clinical trials.</P>

가평 전주이씨묘 출토복식 고찰

최연우,박윤미,김윤경,김지희,박양희,이선우,차서연,편나영,황혜남,Choi, Yeon Woo,Park, Yoon Mee,Kim, Yoon Gyung,Kim, Ji Hee,Park, Yang Hee,Lee, Seon U,Cha, Seo Yeon,Pyeon, Na Young,Hwang, Hye Nam 한국의류학회 2017 한국의류학회지 Vol.41 No.5

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

감마선조사 생쥐의 폐 및 심장조직중의 Superoxide Dimutase 활성도에 대한 연구

이상협,전현우,김우제,양종대,차종희 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

The change of cytosolic superoxide dismutase (E.C.1.15.1.1. ) activities in the lung and heart of mice irradiated with a single dose of gamma irradiation 400 rads for the whole body was examined. The specific activities of superoxide dismutase in cytosol fraction of lung and heart were decreased to lowest levels on 7 day after irradiation and the activities of superoxide dismutase per gram of the organ tissues were decreased to lowest level on 21 day after irradiation. Also the total activities of superoxide dismutase per lung and heart were decreased to lowest level on 7 day after irradiation, It is concluded that the superoxide dismutase activity in cytosol of heart is more decreased than the activity in cytosol of lung after irradiation.

强靷한 모델 追從 制御系의 構成에 관한 硏究

李良雨,車得根 東義大學校産業技術開發硏究所 1992 産業技術硏究誌 Vol.6 No.-

This paper investigates the problem of designing a compensating control for a linear multivariable system so that the closed-loop system behaves like a specified ideal system or model. Synthesis technique through linear optimal control theory is applied to a model following problem, for the plant which has contant disturbances. To meet the control objectives, a new type quadratic performance index is proposed and simplified the problem using linear regulator problem in which the integral quadratic error between the plant and model outputs is minimized. The optimal system constructed by this metod consists of the both of a feedback controller and a feedforward controller, and its step response can be satisfactorily kept close to that of the model without offset. A numerical example illustrates the efficiency of this system.

자일리톨, 후노란, 제2인산칼슘 및 카제인포스포펩타이드배합껌이 법랑질의 광질재침착에 미치는 영향에 관한 실용실험연구

이병진,배광학,박우철,양준영,황수정,노정,백대일,김종배 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.4

The object of this study was to investigate caries preventive effect of chewing gum containing xylitol, funoran, calcium hydrogen phosphate and casein phosphopeptide by examining Vickers' hardness and surface roughness with confocal laser microscope. Early caries-like lesions were created using 0.1 M lactic acid-sodium hydroxide buffer(pH 4.0). 8 pieces of 3am diameter bovine enamel specimen with early caries-like lesions were mounted into the recesses of lingual surface of the acrylic mandibular removable appliance. 13 subjects were the appliance for daytime and chewed control and experimental gums 7 times in a day, for 3 days. In order to evaluate the remineralization effect of chewing gum on specimens, we measured vickers' hardness number after chewing gum, 1 day, 2 days, 3 days after and calculated surface roughness immediately after chewing gum. Vickers' hardness number of experimental chewing gum was significantly higher than control in 2 and 3 days after. Surface roughness of experimental chewing gum was significantly lower than control immediately after chewing gum. These results showed that experimental gum has more effective in remineralization of bovine tooth enamel, and it is more effective at immediately after chewing.

大腸洗滌이 血液의 有形成分에 미치는 影響 : 육상선수를 중심으로

우재학,이복식,하현보,윤양진,이종환 釜山大學校 附設 體育科學硏究所 1987 體育科學硏究所 論文集 Vol.3 No.-