Protective Effect of Polysaccharide Fractions from Radix A. Sinensis against tert-Butylhydroperoxide Induced Oxidative Injury in Murine Peritoneal Macrophages

Yang, Xingbin,Zhao, Yan,Lv, You,Yang, Ying,Ruan, Yun Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

Three Angelica sinensis polysaccharide fractions (APFs), named APF1, APF2 and APF3, were isolated and purified from Radix A. sinensis and their antioxidant activities were evaluated in isolated mouse peritoneal macrophages by pretreatment with APFs before exposure to 0.2 mM tertbutylhydroperoxide (t-BHP). The results showed that pretreatment of the macrophages with APFs as low as $10{\mu}g$/ml could significantly enhance t-BHP-decreased cell survival, intracellular glutathione (GSH) content and superoxide dismutase (SOD) activity, and also inhibited t-BHP-increased lactate dehydrogenase (LDH) leakage and malondialdehyde (MDA) formation (p < 0.05), and APF3 was the most active fraction, followed by APF2 and APF1 in decreasing order. Furthermore, we found for the first time that the bound-protein in APF3 was associated closely with the protective effects and the polysaccharide inhibited the excess NO release from t-BHP-activated macrophages to protect host cells.

Positive Association of TEAD1 With Schizophrenia in a Northeast Chinese Han Population

Yang Sun,Lin Wen,Yi-Yang Luo,Wen-Juan Hu,Hui-Wen Ren,Ye Lv,Cong Zhang,Ping Gao,Li-Na Xuan,Guan-Yu Wang,Cheng-Jie Li,Zhi-Xin Xiang,Zhi-Lin Luan 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.12

Objective Schizophrenia is a complex and devastating psychiatric disorder with a strong genetic background. However, much uncertainty still exists about the role of genetic susceptibility in the pathophysiology of schizophrenia. TEA domain transcription factor 1 (TEAD1) is a transcription factor associated with neurodevelopment and has modulating effects on various nervous system diseases. In the current study, we performed a case–control association study in a Northeast Chinese Han population to explore the characteristics of pathogenic <i>TEAD1</i> polymorphisms and potential association with schizophrenia.Methods We recruited a total of 721 schizophrenia patients and 1,195 healthy controls in this study. The 9 single nucleotide polymorphisms (SNPs) in the gene region of <i>TEAD1</i> were selected and genotyped.Results The genetic association analyses showed that five SNPs (rs12289262, rs6485989, rs4415740, rs7113256, and rs1866709) were significantly different between schizophrenia patients and healthy controls in allele or/and genotype frequencies. After Bonferroni correction, the association of three SNPs (rs4415740, rs7113256, and rs1866709) with schizophrenia were still evident. Haplotype analysis revealed that two strong linkage disequilibrium blocks (rs6485989-rs4415740-rs7113256 and rs16911710-rs12364619-rs1866709) were globally associated with schizophrenia. Four haplotypes (C-C-C and T-T-T, rs6485989-rs4415740-rs7113256; G-T-A and G-T-G, rs16911710-rs12364619-rs1866709) were significantly different between schizophrenia patients and healthy controls.Conclusion The current findings indicated that the human <i>TEAD1</i> gene has a genetic association with schizophrenia in the Chinese Han population and may act as a susceptibility gene for schizophrenia.

Biodistribution of a Promising Probiotic, Bifidobacterium longum subsp. longum Strain BBMN68, in the Rat Gut

Lv, Yang,Qiao, Xuewei,Zhao, Liang,Ren, Fazheng The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6

Bifidobacterium longum subsp. longum BBMN68, isolated from centenarians in Guangxi, China, has been proved to be a promising probiotic strain for its health benefits. In this study, the biodistribution of this strain in the rat gut was first investigated using the quantitative realtime PCR assay and propidium monoazide. Strain-specific primers were originally designed based on the BBMN68 genome sequence. Healthy rats were orally inoculated with either a single dose of BBMN68 (10¹⁰ colony-forming units/kg), or with one dose per day for 7 days and bacterial concentrations were analyzed in detail from the intestinal contents and feces of four different gut locations, including stomach, small intestine, colon, and rectum. Results indicated that strain BBMN68 could overcome the rigors of passage through the upper gastrointestinal tract and transiently accumulate in the colon, even though survival in the stomach and small intestine was not high. A good level of BBMN8 could stay in vivo for 72 h following a 7-day oral administration, and a daily administration is suggested for a considerable and continuous population of BBMN68 to be maintained in the host intestine.

Reduced Graphene Oxide-Supported Cobalt Phosphide Nanoflowers via in situ Hydrothermal Synthesis as Pt-Free Effective Electrocatalysts for Oxygen Reduction Reaction

Yang Lv,Xianbao Wang,Tao Mei,Jinhua Li,Jianying Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.5

Cobalt phosphide (CoP) has aroused extensive research interest in a field of electrochemical application due to its excellent catalytic activities. CoP and its compounds have been widely reported using in hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). However, few reports about CoP as electrocatalysts for oxygen reduction reaction (ORR) were presented. In this work, we prepare reduced graphene-oxide(rGO)-loaded CoP (rGO@CoP) as an electrocatalyst for ORR through in situ hydrothermal treatment. The rGO@CoP as ORR catalyst exhibits excellent activities where its onset potential has a positive increase of 129 mV, and the ORR potential achieves an increase of 330mV at a current density of 1.0mA cm -2 compared with that of pure CoP. The current density is also significantly improved with an increase of 0.51mA cm -2 at -350 mV, and the Tafel slope has a decrease of 19mV dec -1. Further tests show that the electron transfer number of rGO@CoP is 3.66, which is larger than 2.19 of pure CoP, indicating that it is dominated by a four-electron transfer pathway. Moreover, its stability (remained 98.6% current after working 6000 s) and methanol tolerance are outstanding. These results show that rGO@CoP may be considered to replace traditional Pt-based ORR catalysts for fuel cells, and rGO loading has been proven to be an effective strategy to enhance the ORR performance of CoP, which may provide a new idea to synthesize transition metal phosphides as ORR catalysts.

Wireless power transmission equipment used for implantable cardiac pacemakers

Shuangqing Lv,Wenjie Chen,Xiufang Hu,Yang Yang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Wireless Power Transfer (WPT) technology has gotten rapid development with its advantages of flexible and convenient. As a safe, reliable and flexible power supply, the introduction of WPT technology promotes the development of implantable medical electronic devices. In this paper, a novel inductive power transfer (IPT) system with redundant parallel receiving coils is proposed to enhance the system’s power capacity and the WPT system is still guaranteed to work properly in the event of a fault in any of the receiving coils. This structure based on the double receiving coil connected in parallel with the load has the advantages of stable working condition, less magnetic flux leakage, and redundant design to ensure safety.

Positive Association of Human SHC3 Gene with Schizophrenia in a Northeast Chinese Han Population

Ye Lv,Yang Sun,Guan-Yu Wang,Jian Yin,Cheng-Jie Li,Yi-Yang Luo,Zhi-Lin Luan 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.9

Objective Schizophrenia is one of the most devastating neuropsychiatric disorders. Genetic epidemiological studies have confirmed that schizophrenia is a genetic disease. Genes promoting neurodevelopment may be potential candidates for schizophrenia. As an adaptor linking a number of tyrosine kinase receptors in multiple intracellular signaling cascades, Src homology 2 domain containing transforming protein 3 (SHC3) is a member of the Shc-like adaptor protein family, and expressed predominantly in the mature neurons of the central nervous system (CNS). In the present study, we aimed to investigate the association of SHC3 and schizophrenia. Methods An independent case-control association study was performed in a sample including 710 schizophrenia patients and 1314 healthy controls from a Northeast Chinese Han population. Results The allelic and genotypic association analyses showed that four SNPs in SHC3 significantly associated with schizophrenia(rs2316280, rs4877041, rs944485 and rs7021743). The haplotype composing of these four SNPs also showed significantly individual and global association with schizophrenia. Conclusion Our present results suggest SHC3 as a susceptibility gene for schizophrenia.

Fingerprint Construction and Genetic Diversity Analysis of Tree Peony Collected from Hunan Province Based on SSR Markers

Zhengmin Yang,Yang Shi,Jiaqi Shuai,Yanlin Li,Tianjiao Sun,Mengjie Chen,Changping Lv 한국원예학회 2021 원예과학기술지 Vol.39 No.5

To protect and identify the varieties of tree peony, DNA fingerprints were constructed and genetic diversity was analyzed with simple sequence repeat markers with tailed primer M13 (TP-M13-SSR) technology in 18 local tree peony varieties in Hunan Province of China. Twenty-four out of 131 pairs of polymorphic primers showing stable amplification and good capillary electrophoresis peaks were selected. The Shannon information indexes (I), polymorphism information contents (PICs), and differentiation rates of eight primer pairs were higher than average. Unweighted pair-group method with arithmetic mean (UPGMA) dendrograms indicated that the genetic similarity coefficients of the 18 Hunan tree peony varieties ranged from 0.57 to 0.86, and Paeonia suffruticosa ‘Ning Xiang Hong’ (0.57) was classified as a separate group. The 18 varieties could be completely distinguished with two pairs of primers. The variety names, flower types, fingerprinting codes, and other information were included in the quick response (QR) code, which provides a basis for the molecular identification of Hunan tree peony varieties. The construction of DNA fingerprinting of tree peony varieties in Hunan is important for its protection.

Bone Morphogenetic Protein 9 Overexpression Reduces Osteosarcoma Cell Migration and Invasion

Zilan Lv,Ya-guang Weng,Dandan Yang,Jie Li,Min Hu,Min Luo,Xiaoqin Zhan,Peipei Song,Chen Liu,Huili Bai,Baolin Li,Yang Yang,Yingying Chen,Qiong Shi 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2

Transforming growth factor- (TGF-) is known to pro-mote tumor migration and invasion. Bone morphogenetic proteins (BMPs) are members of the TGF- family expressed in a variety of human carcinoma cell lines. The role of bone morphogenetic protein 9 (BMP9), the most powerful osteogenic factor, in osteosarcoma (OS) progression has not been fully clarified. The expression of BMP9 and its receptors in OS cell lines was analyzed by RT-PCR. We found that BMP9 and its receptors were expressed in OS cell lines. We further investigated the influence of BMP9 on the biological behaviors of OS cells. BMP9 overexpression in the OS cell lines 143B and MG63 inhibited in vitro cell migration and invasion. We further investigated the ex-pression of a panel of cancer-related genes and found that BMP9 overexpression increased the phosphorylation of Smad1/5/8 proteins, increased the expression of ID1, and reduced the expression and activity of matrix metalloproteinase 9 (MMP9) in OS cells. BMP9 silencing induced the opposite effects. We also found that BMP9 may not affect the chemokine (C-X-C motif) ligand 12 (CXCL12)/C-X-C chemokine receptor type 4 (CXCR4) axis to regulate the invasiveness and metastatic capacity of OS cells. Interestingly, CXCR4 was expressed in both 143B and MG63 cells, while CXCL12 was only detected in MG63 cells. Taken together, we hypothesize that BMP9 inhibits the migration and invasiveness of OS cells through a Smad-dependent pathway by downregulating the expression and activity of MMP9.

Protective Effect of Polysaccharide Fractions from Radix A. Sinensis against tert-Butylhydroperoxide Induced Oxidative Injury in Murine Peritoneal Macrophages

( Xing Bin Yang ),( Yan Zhao ),( You Lv ),( Ying Yang ),( Yun Ruan ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.6

Possible Risk Factors Associated with Radiation Proctitis or Radiation Cystitis in Patients with Cervical Carcinoma after Radiotherapy

Yang, Lin,Lv, Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Radiation proctitis and radiation cystitis are major complications for patients with cervical carcinoma following radiotherapy. In the present study, we aimed to determine the potential risk factors for the development of radiation proctitis and radiation cystitis after irradiation. A total of 1,518 patients with cervical carcinoma received external beam radiotherapy (EBRT) followed by high-dose-rate intracavitary brachytherapy (HDRICB) in our hospital. The incidences of radiation proctitis and radiation cystitis were recorded and associations with different factors (age, time period, tumor stage) were analyzed with ${\chi}^2$ (chi-squared) and Fisher exact tests. We found that 161 and 94 patients with cervical carcinoma were diagnosed with radiation proctitis and radiation cystitis, respectively, following radiotherapy. The prevalence of Grade I-II radiation proctitis or radiation cystitis was significantly lower than that of Grade III (radiation proctitis: 3.82% vs. 6.76%, P < 0.05; radiation cystitis: 2.31% vs. 3.87%, P < 0.05) and was significantly enhanced in patients with late stage (IIIb) tumor progression compared to those in early stage (Ib, IIa) (P < 0.05). Moreover, the incidence of radiation proctitis and cystitis was not correlated with age or, time period following radiation, for each patient (P > 0.05). These observations indicate that a late stage of tumor progression is a potential risk factor for the incidence of radiation proctitis and cystitis in cervical carcinoma patients receiving radiotherapy.