Fertility-preserving treatment outcome in endometrial cancer or atypical hyperplasia patients with polycystic ovary syndrome

Lulu Wang,Xuezhen Luo,Qian Wang,Qiaoying Lv,Pengfei Wu,Wei Liu,Xiaojun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.5

Objective: This study aimed to investigate the impact of polycystic ovary syndrome (PCOS) on fertility-sparing treatment in young patients with atypical endometrial hyperplasia (AEH) or endometrioid endometrial cancer (EEC). Methods: A total of 285 patients with EEC (n=76, FIGO stage IA, without myometrium invasion) or AEH (n=209) who received progestin-based fertility-sparing treatment were evaluated retrospectively. Among the 285 patients, 103 (36.1%), including 70 AEH cases and 33 EEC cases, were diagnosed with PCOS. General characteristics, cumulative 16- and 32-week complete response (CR) rate, pregnancy outcome and recurrence were compared between patients with or without PCOS. Results: The cumulative 16-week CR rate was lower in the PCOS group than in the non- PCOS group (18.4% vs. 33.8%, p=0.006). Patients with PCOS took longer treatment duration to achieve CR (7.0 months vs. 5.4 months, p=0.006) and shorter time to relapse after CR (9.6 months vs. 17.6 months, p=0.040) compared with non-PCOS group. After adjusting for patient age, body mass index, PCOS, homeostasis model assessment-insulin resistance index, and serum testosterone levels, we found that body mass index ≥25 kg/m2 (HR=0.583; 95% CI=0.365–0.932; p=0.024) and PCOS (HR=0.545; 95% CI=0.324–0.917; p=0.022) were significantly correlated with lower 16-week CR rate. Conclusion: PCOS was associated with lower 16-week CR rate, longer treatment duration and shorter recurrence interval in patients with AEH or EEC receiving fertility-preserving treatment.

Bio-based Epoxy Thermoset Containing Stilbene Structure with Ultrahigh T<SUB>g</SUB> and Excellent Flame Retardancy

Guangming Lu,Xuezhen Wang,Na Teng,Jingyuan Hu,Liyue Zhang,Jinyue Dai,Yongjia Xu,Sakil Mahmud,Xiaoqing Liu 한국고분자학회 2021 폴리머 Vol.45 No.4

Bio-based epoxy resins with an ultrahigh glass transition temperature (Tg) and excellent flame retardancy are critical for developing sustainable polymers. Herein, a novel trifunctional epoxy monomer triglycidyl ether of resveratrol (TGER) was synthesized from renewable resveratrol. The chemical structure of TGER was confirmed by Fourier transform infrared (FTIR), ¹H, and <SUP>13</SUP>C nuclear magnetic resonance (NMR) spectroscopy which was then reacted with 4,4’-diaminodiphenylmethane (DDM) to form resin. The obtained resin was evaluated in terms of flame retardance and thermal properties. The resultant TGER-DDM 240 resin shows excellent flame-retardant properties, presenting a residual char of 42.5% at 800 ℃, limiting oxygen index (LOI) of 31.2%, and flammability rating of V-0 in UL94 test. Moreover, the resin possesses an ultrahigh Tg at 294 ℃. This work provides a facile method for preparing high-performance flame-retardant epoxy resin from a renewable resource.

Structure evolution in carbon molecular sieve membranes derived from binaphthol-6FDA polyimide and their gas separation performance

Guoxiong Deng,Yilei Wang,Xueping Zong,Jiangzhou Luo,Xuezhen Wang,Chunxue Zhang,Song Xue 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-

Here we reported a causal relationship between the molecular structure of binaphthol-based polyimideprecursor and the pore-size distribution of the derived carbon membrane. The binaphthol-basedpolyamide acid is synthesized from 2,20-diol-1,10-binaphthyl-6,60-diamine and 4,40-(hexafluoroisopro-pylidene)diphthalic anhydride (6FDA). Then, an azeotropic imidiaztion method was used to synthesizethe polyimide with naphthol groups (XS1). When the imidization is carried out by using acetic anhydride,the polyimide with acetyl groups (XS4) is achieved. The CMS membranes prepared by pyrolyzing XS1 andXS4 at 500, 550, and 600 ℃ are named using the temperature as the suffix, such as XS1-500. Their poreevolution has been investigated using TGA, FTIR, XRD, and Raman measurements. The trimodal pore-sizedistribution is in the carbon molecular sieve (CMS) membranes derived from XS4 and the CMS onesderived from XS1 exhibit a bimodal pore structure. Among them, XS4-500 exhibits the highest gaspermeabilities of 3332 barrer for CO2, 773 barrer for O2, and 119 barrer for N2. XS1-500 only affords theCO2, O2, and N2 permeabilities of 1086, 230, and 30.2 barrer. The esterification of naphthol not justdisturbs the hydrogen bonds between polyimide chains but also affects the pore generation of thederived CMS membranes. Our work provides an effective way to enhance the gas permeability of a CMSmembrane derived from the binaphthol-based polyimide.

Conservative therapy with metformin plus megestrol acetate for endometrial atypical hyperplasia

Weiwei Shan,Chao Wang,Zhenbo Zhang,Chao Gu,Chengcheng Ning,Xuezhen Luo,Qiongjie Zhou,Xiaojun Chen 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.3

Objective: To compare the efficacy of metformin plus megestrol acetate (MA) with that of MA alone for treating endometrial atypical hyperplasia (EAH). Methods: This pilot study included 16 EAH patients who met at least one metabolic syndrome (MS) criterion and received either adjunctive metformin plus MA (MET group) or MA monotherapy (MA group). Each patient in the MA group received 160 mg of MA daily, whereas patients in the MET group received the same dose of MA plus 0.5 g of metformin thrice daily. Treatment response was assessed by histological examination of dilation and curettage specimens obtained after 12 weeks of therapy. Results: Each group had eight patients, and half of the patients in each group were diagnosed with MS. The complete response (CR) rate was 75% (6/8) in the MET group and 25% (2/8) in the MA group (p=0.105). Complications of MS did not affect the response rates in either group. In the MET group, 75% (3/4) of the patients had CR in the presence or absence of MS. In the MA group, 50% (2/4) of the patients with MS had CR, whereas no patient without MS had CR. No irreversible toxicities were observed. Conclusion: Metformin plus MA may be a potential alternative therapy for treating EAH, and the MS status of patients may have no effect on the efficacy of metformin plus MA therapy.

Standardized Treatment and Shortened Depression Course can Reduce Cognitive Impairment in Adolescents With Depression

Penghui Cao,Junjie Tan,Xuezhen Liao,Jinwei Wang,Lihuan Chen,Ziyan Fang,Nannan Pan 대한소아청소년 정신의학회 2024 소아청소년정신의학 Vol.35 No.1

Objectives: This study aimed to explore the influence of depression severity, disease course, treatment status, and other factors on cognitive function in adolescents with depressive disorders. Methods: Participants who met the inclusion criteria were enrolled in the study. Sociodemographic data of each participant were recorded, including age, sex, and family history of mental disorders. Zung’s Self-Rating Depression Scale was used to assess depression status in adolescents. Moreover, P300 and mismatch negativity (MMN) were used to objectively evaluate the participants’ cognitive function. Results: Only 26.8% of the adolescents with depression received standard antidepressant treatment. The latencies of N2 (267.80±23.34 ms), P3 (357.71±32.09 ms), and MMN (212.10±15.61 ms) in the adolescent depression group were longer than those in the healthy control group (p<0.01). Further analysis revealed that the latency of MMN was extended with increased levels of depression in adolescents. The MMN latency was short in participants with depression receiving standardized treatment. Furthermore, the latency of MMN was positively correlated with the severity and duration of depression (correlation coefficients were 0.465 and 0.479, respectively) (p<0.01). Conclusion: Receiving standardized treatment and shortening the course of depression can reduce cognitive impairment in adolescents with depression.

microRNA-506-3p suppresses the proliferation of triple negative breast cancer cells via targeting SNAI2

Zhao Xuye,Bai Xiangdong,Li Weina,Gao Xuezhen,Wang Xiaoli,Li Bin 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.4

Background The lacking of estrogen, progesterone and ERBB2 receptor makes the therapy of Triple negative breast cancer (TNBC) is particularly challenging. Accumulating evidences has demonstrated the dysfunction and critical roles of microRNAs (miRNAs) in TNBC. Objective Explore the role of miR-506-3p in TNBC and evaluate the clinical significance of miR-506-3p. Results miR-506-3p expression was significantly decreased in TNBC and correlated with the worst status of TNBC patients. miR-506-3p overexpression repressed the proliferation, migration and induced cell cycle arrest as well as apoptosis of TNBC cells. Mechanistically, SNAI2 was identified as a target of miR-506-3p in TNBC cells. Consistently, SNAI2 was overexpressed in TNBC and inversely correlated with miR-506-3p level. Transfection of SNAI2 abolished the inhibitory role of miR-506-3p in regulating the malignant phenotypes of TNBC cells. Conclusions These results demonstrated the suppressive function of miR-506-3p in TNBC via targeting SNAI2, indicating the possible application of miR-506-3p in TNBC treatment. Background The lacking of estrogen, progesterone and ERBB2 receptor makes the therapy of Triple negative breast cancer (TNBC) is particularly challenging. Accumulating evidences has demonstrated the dysfunction and critical roles of microRNAs (miRNAs) in TNBC. Objective Explore the role of miR-506-3p in TNBC and evaluate the clinical significance of miR-506-3p. Results miR-506-3p expression was significantly decreased in TNBC and correlated with the worst status of TNBC patients. miR-506-3p overexpression repressed the proliferation, migration and induced cell cycle arrest as well as apoptosis of TNBC cells. Mechanistically, SNAI2 was identified as a target of miR-506-3p in TNBC cells. Consistently, SNAI2 was overexpressed in TNBC and inversely correlated with miR-506-3p level. Transfection of SNAI2 abolished the inhibitory role of miR-506-3p in regulating the malignant phenotypes of TNBC cells. Conclusions These results demonstrated the suppressive function of miR-506-3p in TNBC via targeting SNAI2, indicating the possible application of miR-506-3p in TNBC treatment.

VDR mediated HSD3B1 to regulate lipid metabolism and promoted testosterone synthesis in mouse Leydig cells

Xue Zhen,Zhuang Jianan,Bai Hao,Wang Ling,Lu Hongzhao,Wang Shanshan,Zeng Wenxian,Zhang Tao 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: The vitamin D receptor (VDR) mediates the pleiotropic biological actions that include osteoporosis, immune responses and androgen synthesis wherein the VDR transcriptionally regulates expression of the genes involved in this complex process. 3β-Hydroxysteroid dehydrogenase-1 (HSD3B1) is an absolutely necessary enzyme for androgen synthesis. Objective: The purpose of the present study was to explore the molecular mechanism of VDR mediated HSD3B1 regulation of lipid metabolism and testosterone synthesis. Methods: The levels of VDR, HSD3B1 and lipid metabolism associated protein were determined by quantitative real-time polymerase chain reaction (RT-qPCR) or western blot. The levels of testosterone concentrations in cell culture media serum by enzyme-linked immunosorbent assay (ELISA). Targeted relationship between VDR and Hsd3b1 was evaluated by dual-luciferase reporter assay. Results: Based on the data analysis of mouse testicular proteome, we found that the expression of HSD3B1 was significantly reduced after VDR deletion. Here, we identified that Hsd3b1 was widely expressed in different tissues of mice by RT-qPCR, and was highly expressed in testis, and mainly located in testicular Leydig cells. Dual-luciferase assay confirmed that VDR could bind candidate vitamin D responsive elements (VDREs) in upstream region of Hsd3b1, and enhance gene expression. Furthermore, over-expression VDR and HSD3B1 significantly increased testosterone synthesis in mice Leydig cells. Meanwhile, Lpl expression was significantly down-regulated and Angptl4 expression was significantly up-regulated in the present of HSD3B1 overexpression. Both LPL and ANGPTL4 play important roles in regulating lipid metabolism. Conclusions: The present study unveiled VDR mediated HSD3B1 to regulate lipid metabolism and promoted testosterone synthesis in mouse Leydig cells. These findings will greatly help us to understand the roles of VDR and HSD3B1 in testosterone synthesis and lipid metabolism.