Metabolites from the Fungus Cephalosporium sp. AL031

Bi, Xu-Bin,A, Fang,Zhao, Qian-Rong,Bi, Yun-Mei 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

Metabolites from the Fungus Cephalosporium sp. AL031

Yun-Mei Bi,Xu-Bin Bi,Fang A,Qian-Rong Zhao 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

A new pyrone derivative, 7, 9-dihydroxy-10-methyl-2H, 4aH, 6H, 10bH-pyrano[5,6-c][2]benzopyran- 2,6-dione (1), was isolated from a culture broth of a strain of the fungus Cephalosporium sp. AL031, together with three known compounds, 3-acetyl-7-hydroxy-5-methoxyl-3Hisobenzofuran- 1-one (2), vermopyrone (3), and 5-methylmellein (4). Their structures were elucidated by spectroscopic analysis including MS and 2D-NMR. Compounds 2, 3, and 4 are reported for the first time from fermentation broth of this fungus through the present study.

Identification and Functional Analysis of Differentially Expressed Genes Related to Metastatic Osteosarcoma

Niu, Feng,Zhao, Song,Xu, Chang-Yan,Chen, Lin,Ye, Long,Bi, Gui-Bin,Tian, Gang,Gong, Ping,Nie, Tian-Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: To explore the molecular mechanisms of metastatic osteosarcoma (OS) by using the microarray expression profiles of metastatic and non-metastatic OS samples. Materials and Methods: The gene expression profile GSE37552 was downloaded from Gene Expression Omnibus database, including 2 human metastatic OS cell line models and 2 two non-metastatic OS cell line models. The differentially expressed genes (DEGs) were identified by Multtest package in R language. In addition, functional enrichment analysis of the DEGs was performed by WebGestalt, and the protein-protein interaction (PPI) networks were constructed by Hitpredict, then the signal pathways of the genes involved in the networks were performed by Kyoto Encyclopaedia of Genes and Genomes (KEGG) automatic annotation server (KAAS). Results: A total of 237 genes were classified as DEGs in metastatic OS. The most significant up- and down-regulated genes were A2M (alpha-2-macroglobulin) and BCAN (brevican). The DEGs were significantly related to the response to hormone stimulus, and the PPI network of A2M contained IL1B (interleukin), LRP1 (low-density lipoprotein receptor-related protein 1) and PDGF (platelet-derived growth factor). Furthermore, the MAPK signaling pathway and focal adhesion were significantly enriched. Conclusions: A2M and its interactive proteins, such as IL1B, LRP1 and PDGF may be candidate target molecules to monitor, diagnose and treat metastatic OS. The response to hormone stimulus, MAPK signaling pathway and focal adhesion may play important roles in metastatic OS.

Improvement of the Beam-Wave Interaction Eciency Based on the Coupling-Slot Configuration in an Extended Interaction Oscillator

Sairong Zhu,Yong Yin,Liangjie Bi,Zhiwei Chang,Che Xu,Fanbo Zeng,Ruibin Peng,Wen Zhou,Bin Wang,Hailong Li,Lin Meng 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.9

A method aimed at improving the beam-wave interaction eciency by changing the coupling slot configuration has been proposed in the study of extended interaction oscillators (EIOs). The disper- sion characteristics, coupling coecient and interaction impedance of the high-frequency structure based on different types of coupling slots have been investigated. Four types of coupled cavity structures with different layouts of the coupling slots have been compared to improve the beam- wave interaction eciency, so as to analyze the beam-wave interaction and practical applications. In order to determine the improvement of the coupling slot to a coupled cavity circuit in an EIO, we designed four nine-gap EIOs based on the coupled cavity structure with different coupling slot configurations. With different operating frequencies and voltages takes into consideration, beam voltages from 27 to 33 kV have been simulated to achieve the best beam-wave interaction eciency so that the EIOs are able to work in the 2 mode. The in uence of the Rb and the ds on the output power is also taken into consideration. The Rb is the radius of the electron beam, and the ds is the width of the coupling slot. The simulation results indicate that a single-slot-type EIO has the best beam-wave interaction eciency. Its maximum output power is 2.8 kW and the eciency is 18% when the operating voltage is 31 kV and electric current is 0.5 A. The output powers of these four EIOs that were designed for comparison are not less than 1.7 kW. The improved coupling-slot con- gurations enables the extended interaction oscillator to meet the different engineering requirements better.

Optimal Configuration of Flexible Interconnection Devices for Transferring Photovoltaic Power in Active Distribution Network

Wu Hongbin,He Ye,Lin Xueshan,Bi Rui,Hua Yuting,Sun Ming,Xu Bin 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2

With the increasing penetration of distributed photovoltaic in distribution network, it is more difficult to control active distribution network (ADN). A flexible interconnection device (FID) can realize regional interconnection of the ADN through transferring power. However, the influence of installation position and number of FIDs on the ADN varies, it is necessary to analyze its operational planning model. In this study, first, a photovoltaic power and load forecasting model is established, followed by reduction of the typical scenarios. Second, the ADN operational planning model is established according to the line load balance index to analyze the coordination and determine the power transfer value of the FID in different installation positions and numbers for the ADN. Third, the net income index of the distribution network in the entire life cycle is established to analyze the economy of the ADN and determine the optimal FID installation location and quantity. Finally, numerous simulations and comparisons are carried out on the actual example system, and the results show that the FID can effectively transfer the active power, strengthen the ADN power supply level, and solve the problem of power supply imbalance caused by distributed photovoltaic access.

Knockdown of glycogen phosphorylase and glycogen synthase influences expression of chitin synthesis genes of rice brown planthopper Nilaparvata lugens

Dao-Wei Zhang,Hui-Juan Wang,Xing Jin,Bi-Ying Pan,Bo-Ping Zeng,Zhong-Jiu Xiao,Cai-Di Xu,Bin Tang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in the glycogen synthesis pathway, which catalyze trehalose and glucose transformation in insects. GS and GP can be regulated by trehalose metabolism, which plays an important role in insect growth. However, it is not known whether these genes can be targeted for pest control through regulation of chitin metabolism. We studied the function of Nilaparvata lugens GS and GP (NLGS and NLGP, respectively) using RNA interference, and reported that trehalose and the chitin biosynthesis pathways are regulated by GP and GS, especially TPS3, TRE1-1, and G6PI1, which decreased following knockdown of these two genes. The expression levels of TPS1, TPS2, and several chitin synthesis pathway family genes were significantly increased following dsNlGP injection. Additionally, despite there being no apparent change to the chitin content, an abnormal molting phenotype and wing deformity appeared, and close to 25% insects died. These results demonstrate that silencing of NLGP or NLGS can lead to molting deformities and an elevated mortality rate through the regulation of chitin pathway genes and chitinase genes. NLGP may play a key role in chitin synthesis due to the number of genes regulated, and higher deformity and mortality rates resulting from its knockdown.

Study on Anti-Proliferative Activity in Cancer Cells and Preliminary Structure–Activity Relationship of Pseudo-Peptide Chiral Thioureas

Peng Liao,Shi-Qin Hu,Hong Zhang,Liang-Bi Xu,Jing-Zi Liu,Bin He,Shang-Gao Liao,Yong-Jun Li 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.3

In our previous studies, we have shown that thiourea compounds containing phosphate esters have potent antitumor activity and can be used as a novel strategy for the development of antitumor agents. Herein, a series of novel phosphonate thioureas 5–38 have been synthesized, which were fully characterized by 1H NMR, 13C NMR spectrum, elemental analysis. Three human cancer cell lines (Bcap-37, BGC-823, and PC-3) have been used to investigate these compounds’ antitumor activities. After the summarization of the structure–activity relationships, we found that the variation of R, R1, and R2 in these novel phosphonate thioureas contribute to the antitumor activities. All these SAR-guided efforts may lead to novel antitumor drugs in the market in the near future.