Antiviral effects of Bovine antimicrobial peptide against TGEV in vivo and in vitro

Xiuli Liang,Xiaojun Zhang,Kaiqi Lian,Xiuhua Tian,Mingliang Zhang,Shiqiong Wang,Cheng Chen,Cunxi Nie,Yun Pan,Fangfang Han,Zhanyong Wei,Wen-Ju Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.5

Background: In suckling piglets, transmissible gastroenteritis virus (TGEV) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. This study explored methods of preventing or inhibiting their production. Bovine antimicrobial peptide-13 (APB-13) has antibacterial, antiviral, and immune functions. Objectives: This study analyzed the efficacy of APB-13 against TGEV through in vivo and in vitro experiments. Methods: The effects of APB-13 toxicity and virus inhibition rate on swine testicular (ST) cells were detected using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). The impact of APB-13 on virus replication was examined through the 50% tissue culture infective dose (TCID50). The mRNA and protein levels were investigated by real-time quantitative polymerase chain reaction and western blot (WB). Tissue sections were used to detect intestinal morphological development. Results: The safe and effective concentration range of APB-13 on ST cells ranged from 0 to 62.5 μg/mL, and the highest viral inhibitory rate of APB-13 was 74.1%. The log10TCID50 of 62.5 μg/mL APB-13 was 3.63 lower than that of the virus control. The mRNA and protein expression at 62.5 μg/mL APB-13 was significantly lower than that of the virus control at 24 hpi. Piglets in the APB-13 group showed significantly lower viral shedding than that in the virus control group, and the pathological tissue sections of the jejunum morphology revealed significant differences between the groups. Conclusions: APB-13 exhibited good antiviral effects on TGEV in vivo and in vitro.

Electroanalytical Approach for Determination of Tanshinone IIA Based on Electrochemically Reduced Graphene Oxide Modified Gold Nanoparticles-Incorporated Carbon Paste Electrode

Xiuli Zhang,Jianwei Li,Dong Wang,Huiqin Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.1

In this work, a novel electrochemical sensor was presented for Tanshinone IIA determination. The as-prepared electrode was prepared by electrodepositing a reduced graphene oxide film onto a carbon paste electrode modified with gold nanoparticles, which combined the advantages of both gold nanoparticles and electrochemically reduced graphene oxide. It was characterized with scanning electron microscopy and cyclic voltammetry. The electrochemical behavior of Tanshinone IIA at the modified electrode was investigated for the first time. Results revealed that the modified electrode can effectively increase response toward Tanshinone IIA by significantly enhancing the redox peak currents and decreasing the peak-to-peak separation. Under the optimized conditions, the modified electrode showed a linear voltammetric response to Tanshinone IIA at concentrations ranging from 5.0 x 10-8 mol L-1 to 1.0 x 10-5 mol L-1, with a detection limit of 1.0 x 10-8 mol L-1 (S/N = 3). The proposed method was successfully applied in determination of Tanshinone IIA in pharmaceutical samples with satisfactory results.

Field measurement and numerical simulation of excavation damaged zone in a 2000 m-deep cavern

Zhang, Yuting,Ding, Xiuli,Huang, Shuling,Qin, Yang,Li, Peng,Li, Yujie Techno-Press 2018 Geomechanics & engineering Vol.16 No.4

This paper addresses the issue of field measurement of excavation damage zone (EDZ) and its numerical simulation method considering both excavation unloading and blasting load effects. Firstly, a 2000 m-deep rock cavern in China is focused. A detailed analysis is conducted on the field measurement data regarding the mechanical response of rock masses subjected to excavation and blasting operation. The extent of EDZ is revealed 3.6 m-4.0 m, accounting for 28.6% of the cavern span, so it is significantly larger than rock caverns at conventional overburden depth. The rock mass mechanical response subjected to excavation and blasting is time-independent. Afterwards, based on findings of the field measurement data, a numerical evaluation method for EDZ determination considering both excavation unloading and blasting load effects is presented. The basic idea and general procedures are illustrated. It features a calibration operation of damage constant, which is defined in an elasto-plastic damage constitutive model, and a regression process of blasting load using field blasting vibration monitoring data. The numerical simulation results are basically consistent with the field measurement results. Further, some issues regarding the blasting loads, applicability of proposed numerical method, and some other factors are discussed. In conclusion, the field measurement data collected from the 2000 m-deep rock cavern and the corresponding findings will broaden the understanding of tunnel behavior subjected to excavation and blasting at great depth. Meanwhile, the presented numerical simulation method for EDZ determination considering both excavation unloading and blasting load effects can be used to evaluate rock caverns with similar characteristics.

Sustainable Agricultural Branding in Digital Asia: A Comparative Analysis of TWG Singapore and the Tea Industry in Guizhou, China

Xiuli Chen,Kesu Lin,Zeyuan Wang,Wei Ding,Xueying Zhang,Hongying Zhu,Tao Wang,Joohan Ryoo KOREA INSTITUTE FOR HUMANITIES AND SOCIAL SCIENCES 2022 Journal of Global Area Studies Review(GAS) Vol.1 No.1

The sedimentary environment of Early Cretaceous rift basin in eastern China and its response to the Faraoni event

Xiangyu Zhang,Shoujun Li,Xiuli Zhao,Geng Geng,Mingming Yan 한국지질과학협의회 2020 Geosciences Journal Vol.24 No.4

To better understand the sedimentary environment of the rift basin under the influence of a warm global climate, Lingshan Island in eastern Shandong Province (China) was studied. Inorganic geochemical indexes (B, equivalent B, B/Ga, Ga, V, Couch’s palaeosalinity, Adams’s palaeosalinity, Sr/Ba, Sr/Cu, Rb/Sr, Al2O3/MgO, and CaO/MgO·Al2O3) were analyzed and a quantitative calculation of palaeosalinity was carried out based on the Adams and Couch methods. The sedimentary environment of the rift basin at Lingshan Island was determined according to the morphology and distribution of its palaeobiota as well as inorganic geochemical indexes. We demonstrate that eastern China had high-temperature drought-like conditions during the Early Cretaceous, which may have been influenced by the Faraoni event. The dry-hot climate transformed the rift basin at Lingshan Island into saline lacustrine basin. Therefore, the salinization of water was not influenced by seawater but was the response of terrestrial strata to the warm, dry climate. This study helps to understand the sedimentary background of Cretaceous rift basins in eastern China and the influence of a warm climate on China’s terrestrial strata.

Digital Application of Intangible Cultural Heritage from the Perspective of Cultural Ecology

Jing, Xiuli,Tan, Fang,Zhang, Mu Smart Tourism Research Center 2021 Journal of smart tourism Vol.1 No.1

This paper explored the digital application of intangible cultural heritage from the perspective of cultural ecology. Through field investigations, combined with cultural ecology theory, an ontology-based semantic web technology was proposed, and Nanjing "Yunjin" brocade weaving technique was selected as the research object. The specific steps were as follows: First, based on the field surveys and cultural ecology theory, the intangible cultural ecological environment was divided into natural and social environments. Next, constructing the intangible cultural heritage ontology was constructed, including the collection and collation of Nanjing Yunjin weaving technique knowledge corpus, based on user needs analysis and corpus analysis, CIDOC CRM was used to create rules to build the ontology. Finally, based on the MediaWiki platform and Semantic MediaWiki, the semantic web model of the intangible cultural heritage was designed, and its semantic retrieval function was realized, thereby achieving the practical application of intangible cultural heritage digitization. Based on the perspective of cultural ecology, a set of intangible digital application models was proposed, which expanded the digital application of the cultural ecology theory, verified the application of this model in the sustainable development of cultural tourism, and provided reference for the sustainable development of cultural tourism.

An efficient estimation for the parameter in additive partially linear models with missing covariates

Wang Xiuli,Song Yunquan,Zhang Shuxia 한국통계학회 2020 Journal of the Korean Statistical Society Vol.49 No.3

In this paper, we study the weighted quantile average estimation technique for the parameter in additive partially linear models with missing covariates, which is proved to be an efficient method. The proposed method is based on optimally combining information over different quantiles via multiple quantile regression. We establish asymptotic normality of the weighted quantile average estimators when the selection probability is known, estimated using the non-parametrical method and parametrical method, respectively. Moreover, we compute optimal weights by minimizing asymptotic variance and then obtain the corresponding optimal weighted quantile average estimators. To examine the finite performance of our proposed method, we use the numerical simulations and apply to model time sober for the patients from a rehabilitation center. Simulation results and data analysis further verify that the proposed method is an efficient and safe alternative to both theWCQRmethod andWLSmethod.

A Study on the Influence of Subjective Exercise Experience on Exercise Persistence of the Elderly

Zijian Zhao,Xiuli Zhang,Yue Chen 아시아건강운동학회 2019 Journal of Asian Society for Health & Exercise Vol.1 No.1

PURPOSE: This paper explores the influence of subjective exercise experience on the persistence of exercise in the elderly in China, and helps to make decisions on how to further improve the persistence of the elderly group to participate in physical exercise. METHODS: The data is randomly collected from a sample of 100 elderly people engaged in physical exercise who were invited to participate in the interview and fill in questionnaires; and the data is processed and analyzed according to two scales, which are "Subjective Exercise Experience Scale" and "Exercise Adherence Scale" in various regions of Henan Province, China. RESULTS: The positive well-being dimensions in the subjective exercise experience of the elderly are positively correlated with the five dimensions of persistence, exercise interest, value judgment, cognitive choice and exercise effort in exercise persistence. There is a significant negative correlation between the psychological distress dimension and the persistence, exercise interest, value judgment, and cognitive choice dimensions in exercise persistence(p <0.01); fatigue dimension is significantly negative correlative with the persistence dimension (p < 0.05) of the exercise persistence; the subjective exercise experience of the elderly has a significant predictive power for their exercise persistence, but there are differences in the predictive power of the various dimensions of exercise persistence. CONCLUSIONS: Positive well-being can positively predict the five dimensions of exercise persistence; psychological distress can negatively predict persistence, exercise interest, value judgment and cognitive choice dimension in exercise persistence. Therefore, by improving positive well-being and reducing psychological annoyance, it can effectively improve the persistence of exercise for the elderly, and thus promote the healthy development of the elderly.

PM2.5 in poultry houses synergizes with Pseudomonas aeruginosa to aggravate lung inflammation in mice through the NF-κB pathway

Meng Li,Xiuli Wei,Youzhi Li,Tao Feng,Linlin Jiang,Hongwei Zhu,Xin Yu,Jinxiu Tang,Guozhong Chen,Jianlong Zhang,Xingxiao Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: High concentrations of particulate matter less than 2.5 µm in diameter (PM2.5) in poultry houses is an important cause of respiratory disease in animals and humans. Pseudomonas aeruginosa is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. When excessively high concentrations of PM2.5 in poultry houses damage the respiratory system and impair host immunity, secondary infections with P. aeruginosa can occur and produce a more intense inflammatory response, resulting in more severe lung injury. Objectives: In this study, we focused on the synergistic induction of inflammatory injury in the respiratory system and the related molecular mechanisms induced by PM2.5 and P. aeruginosa in poultry houses. Methods: High-throughput 16S rDNA sequence analysis was used for characterizing the bacterial diversity and relative abundance of the PM2.5 samples, and the effects of PM2.5 and P. aeruginosa stimulation on inflammation were detected by in vitro and in vivo. Results: Sequencing results indicated that the PM2.5 in poultry houses contained a high abundance of potentially pathogenic genera, such as Pseudomonas (2.94%). The lung tissues of mice had more significant pathological damage when co-stimulated by PM2.5 and P. aeruginosa, and it can increase the expression levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α through nuclear factor (NF)-κB pathway in vivo and in vitro. Conclusions: The results confirmed that poultry house PM2.5 in combination with P. aeruginosa could aggravate the inflammatory response and cause more severe respiratory system injuries through a process closely related to the activation of the NF-κB pathway.

Nano-Hydroxyapatite Modified by Grafting Polylactide and its Tissue Engineering Application

Xuesi Chen,Xiuli Zhuang,Peibiao Zhang,Xueyu Qiu,Xiabin Jing 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10