CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

전기영동을 이용한 한국산 다슬기과(Pleuroceridae)의 계통분류

김종환,정우석 충남대학교 기초과학연구소 1995 忠南科學硏究誌 Vol.22 No.1

Electrophoretic analysis has been used frequently to quantify genetic variability in many organisms. Isozyme variation between species of Pleuroceridae was analyzed by an electrophoretic technique. Pleuroceridae is consisted of two genus (Semisulcospira and Koreanomelania) and each genus is consisted of six species and three species, respectively. Among the Pleuroceridae, two genus and six species were analyzed. Each species of Pleuroceridae was collected in Nam-hangang and Kumgang. In SDS-PAGE patterns there are no distinctive differences among species. But in isozyme analysis using 7 isozymes (Esterase, Acid phosphatase, Alkaline phosphatase, Malate dehydrogenase, Alcohol dehydrogenase, Catalase, Peroxidase) of them. Semisulcospira libertina and S. coreana are the most highly related (0.86). S. gottschei is the least lowly related (0.44). Koreanomelania globus (0.62) is more related than S. tegulata (0.52) but less related than S. forticosta (0.67). The order of similarity relationship from S. libertina is S. coreana, S. forticosta, K. globus, S. tegulata and S. gottschei.

백금-코발트 합금박막을 이용한 측온저항체 온도센서의 제작

정귀상,홍석우,박정도 東西大學校 1998 동서논문집 Vol.4 No.-

Platinum-Cobalt alloy thin films were deposited on Al₂O₃substrates by r.f. cosputtering for RTD temperature sensors. We made Pt-Co alloy resistance patterms on the Al₂O₃substrates by lift-off method and investigated the physical and electrical characteristics of these films under various conditions (the input power, working vacuum, annealing temperature, thickness of thin films) and also after annealing these films. At input power of Pt: 44 W/㎠, Co: 6.91 W/㎠, working vacuum of 10 mTorr and annealing conditions of 1000℃ and 60 min, the resistivity and sheet resistivity of Pt-Co thin films was 15 μΩ·cm and 0.5 Ω/□, respectively. The TCR value of Pt-Co alloy thin films was measured with various thickness of thin films and annealing conditions. The optimum TCR value is gained under conditions 3000 A of thin films thickness and 1000℃ of annealing temperature. These results indecate that Pt-Co alloy thin films have potentiality for the high resolution RTD temperature sensors.

웅천천의 대형 저서 무척추동물의 분포와 다양성에 관하여

金鍾煥,趙京和,鄭寓碩 충남대학교 기초과학연구소 1996 忠南科學硏究誌 Vol.23 No.2

To investigate the distribution pattern of the benthic macroinvertebrates in Ungchon stream, this study was carried out at the six selected stations from August to October, 1994. The relationship of macrobenthos to environmental factors was also discussed and the results are summarized as follows: 1. The macrobenthos collected in this study was classified into 4 phyla, 6 classes, 15 orders, 25 families and 38 species, in which auquatic insect larvae were 26 species (68.4%), molluscs 8 species (21.1%), annelids 3 species (7.9%), and plathyhelminthes 1 species (2.6%). 2. It seemes to be few differences in species composition of the macrobenthos with monthly fluctuation or the stations. At station 3, more number of species(20 sp.) were collected than other stations, however less individuals were observed, especially the August, insect larvae would have collected. 3. Based on dominant indices, richness index and evenness index, Semisulcospira libertina and Ecdyonurus yosidae predominated at all stations, while Corbicula papyracea shows at the 3 station. 4. The relationship between collected species and water quality, Limnodrius gotoi which the lower 4th rank species occurred at 1, 2 stations. Hirudo nipponia, Caenis Kua, Chironomus sp.(white), Cloeon dipterum, Cercion calamorim, Orthetrum albitylum speciosum which the 3rd rank species, occurred at the same stations. Phagocata vivida which the 1st rank species were collected at 5 station. The evaluation between evenness and richness indices indicated some relationships, particularly the highest richness and evenness indices showed at station 3. 5. Considering the macrobenthos with the stream structure, Pleuroceridae and Heptageniidae larva predominated in the upper and middle stream at 4, 5, 6 were the stream surface was covered with medium sand. Corbicula papyracea species occurred at 1, 2 stations where the down stream surface was covered with fine sand and clay.

가토의 하악골 연장시 Sodium fluoride 투여가 골형성에 미치는 영향

김용하,설정현,변우목,김정철,우상현,강무석 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.4

The purpose of this study was to improve bone formation and shorten the period involved in distraction osteogenesis using sodium fluoride (NaF). This has been used in managing osteoporosis for several decades. This study was carried out on 40 New Zealand white rabbits. Twenty animals were used for a control group (no medication) and 20 for the experimental group. NaF 1 mg/kg/day was administered to each experimental animal via drinking water beginning 4 weeks prior to surgery and continued until sacrifice for examination. A distraction device was applied to the left mandible along a plane perpendicular to the direction of osteotomy. After a 5-day latent period, the mandibles were lengthened at 1 mm per 24 hours for 10 days. Five rabbits from each group were sacrificed on the 1st day, 2nd week, 4th week and 8th week of the consolidation period. After lengthening, all the rabbits developed a severe cross-bite. By the 4th week, cortical continuity was seen. Bone densities at the site of bony lengthening were measured by quantitative computer tomography (QCT) and dual X-ray absorptiometry (DXA). The results were as follows: the measurements of the experimental group were significantly higher than of the control group by the 1st day of the consolidation period (p<0.05) and insignificantly higher after the 2nd week. Histologically, in the experimental group, there were more osteoblasts, increased and thickened trabeculae and more mature lamellar bones than in the control group. This study showed that osteogenesis of the experimental group was significantly higher during and immediately after distraction. Our study suggested that the use of NaF could improve bone formation and decrease the period of distraction osteogenesis if a safe dose and method of fluoride administration can be determined for growing humans.

Bovine botulism outbreak associated with incidental consumption of presumably contaminated leftover food

( Jae Won Byun ),( Kyung Hyun Lee ),( Ha Young Kim ),( Soon Seek Yoon ),( Ji Youl Jung1 ),( Woo Seog Jeong1 ),( Jae Ku Oem ),( Jong Soo Lim ),( Myoung Heon Lee ),( You Chan Bae ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

Nine cattle among 18 in a native Korean herd died or were euthanized within 3 days. The affected cattle showed cowshed. The pigs and dogs had been fed leftover food originating from nearby restaurants, and the cattle could presumably reach the contaminated food in the pig troughs. Necropsy revealed a few chicken bones and red pepper pieces as well as large amounts of grain and rice straw hay mixed in the rumen. Botulism type D was isolated in the ruminal contents of one of the cattle. We speculated that the outbreak was associated with the cattle incidentally eating presumably contaminated leftover food from the pig trough.

Immunogenicity and Simultaneous Expression of Two Virus-like Particles of Porcine Parvovirus and Encephalomyocarditis Virus

Woo seog Jeong,Hye Young Jeoung,Cheong Up Choi,Hwan Won Choi,Dong Jun An 대한수의학회 2009 대한수의학회 학술대회발표집 Vol.2009 No.-

Epidemiological Investigation on Botulism of Cattle in Northern Gyeonggi-do between 2011 and 2012

Woo seog Jeong,Jeong Kyoung Bae,Woo Jin Jeon,Youn Ju Kim,Ha chung Yoon,Woo Seok Chang,Sung Chul Hwang,Jee Hye Kim,Yong Ju Lee,Myeong Soo Kim,Do Soon Kim,Yi Seok Joo,So Young Park,Oun Kyong Moon 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-

Which is the most accurate parameter to estimate the pulmonary wedge pressure in critically ill patients?

Woo Hyung Bae,June Hong Kim,Jun Hyuk Oh,Dong Won Lee,Sung Gook Song,Jeong Su Kim,Hyeon Gook Lee,Woo Seog Ko,Yong Hyun Park,Sang Gwon Lee,Kook Jin Chun,Taek Jong Hong,Yung Woo Shin 대한내과학회 2004 대한내과학회 추계학술대회 Vol.2004 No.1