Genome-wide Association Studies for Osteoporosis: A 2013 Update

Hong-Wen Deng,Lei Zhang,Christopher J. Papasian,Hong-Wen Deng 대한골대사학회 2014 대한골대사학회지 Vol.21 No.2

In the past few years, the bone field has witnessed great advances in genome-wide as-sociation studies (GWASs) of osteoporosis, with a number of promising genes identified. In particular, meta-analysis of GWASs, aimed at increasing the power of studies by com-bining the results from different study populations, have led to the identification of nov-el associations that would not otherwise have been identified in individual GWASs. Re-cently, the first whole genome sequencing study for osteoporosis and fractures waspublished, reporting a novel rare nonsense mutation. This review summarizes the im-portant and representative findings published by December 2013. Comments are madeon the notable findings and representative studies for their potential influence and im-plications on our present understanding of the genetics of osteoporosis. Potential limi-tations of GWASs and their meta-analyses are evaluated, with an emphasis on under-standing the reasons for inconsistent results between different studies and clarificationof misinterpretation of GWAS meta-analysis results. Implications and challenges ofGWAS are also discussed, including the need for multi- and inter-disciplinary studies.

Differential antioxidation activities in two alfalfa cultivars under chilling stress

Wen-Bin Wang,김윤희,Haeng-Soon Lee,Xi-Ping Deng,Sang-Soo Kwak 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4

To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at 10C. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

Novel HMW glutenin genes from Aegilops tauschii and their unique structures

Wen-Jie Chen,Zhong-Wei Yuan,Lian-Quan Zhang,Xing Fan,Ze-Hong Yan,Ji-Rui Wang,You-Liang Zheng,Huai-Gang Zhang,Deng-Cai Liu 한국유전학회 2012 Genes & Genomics Vol.34 No.3

A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3t and 1Dy12.1**t were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3t was between those of 1Bx6 and 1Bx7, while 1Dy12.1**t with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3t and 1Dy12.1**t were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues,respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3t is the shortest of the known Dx-type alleles. 1Dy12.1**t is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1**t may be a key site in the genealogy of the Glu-Dy.

Study on Chinese Consumers’ Purchase Intention on Cosmetics PPL in Korean(Hallyu) Drama

Deng Xiao-Wen,Soon-Hong Kim 한국유통과학회 2015 KODISA ICBE (International Conference on Business Vol.2015 No.-

In terms of an implication of this study, it was confirmed that hallyu celebrity-oriented imitative psychology, South Korean cosmetics brand image, PPL attitude and others have effects on purchase intention on South Korean cosmetic products and in particular, of the path coefficients, this PPL attitude variable was proved to be the most influential factor and after all, PPL strategies of South Korea’s dramas were verified as a marketing strategy that would work effectively on Chinese consumers. South Korea needs to keep creating cultures and dramas of good quality, and they also must come up with strategies to promote their creations to China.

MiR-214 inhibits apoptosis in thyroid epithelial follicular cells induced by amiodarone through the FASL/MAPK pathway

Wen Jing,Deng Chaonan,Shi Lixin,Zhou Shi,Zhang Miao,Hu Xiaoli,Wang Nianxue,Luo Lijuan 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.2

Background Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is one of the most common autoimmune disease (AITD) in clinical practice. It is urgent to explore the mechanism of amiodarone-induced thyroid dysfunction. Objective This study aims to assess the expression levels of miR-214 and FasL in amiodarone contact type of HT, and the effect of miR-214 on cell viability and apoptosis and potential mechanism. Results We found that miR-214 was low expressed in the tissues of amiodarone-treated thyroiditis patients. MiR-214 increased the survival rate of amiodarone-induced thyroid epithelial follicular cells and inhibited apoptosis. Mechanically, we found that miR-214 could bind to FASL and regulate MAPK signaling pathway through FASL. Conclusions Our results suggested that miR-214 could be a potential therapeutic target for Hashimoto's thyroiditis.

Concept and Analysis of Stay Cables with a CFRP and Steel Composite Section

Wen Xiong,C.S. Cai,Rucheng Xiao,Lu Deng 대한토목학회 2012 KSCE Journal of Civil Engineering Vol.16 No.1

This paper describes the concept of a novel stay cable with a Carbon Fiber Reinforced Plastic (CFRP) and steel composite section. In this concept CFRP core conserves all the advantages of CFRP materials such as light weight and high strength, while steel coat provides protection for the CFRP core and ensures reliable anchorage performance. The steel coat can reduce cost and provide higher stiffness than CFRP materials in some length range. Following this concept, several configurations were proposed for the composite structure. Effects of the key design parameter of the proposed stay cables, namely the ratio of the CFRP section area to the whole section area, were examined through a parametric study using analytical solutions. By doing this, an appropriate range of the ρ value was suggested. Factors that can affect the appropriate range of the ρ value, including the horizontal projected length of stay cables,cable force, and pylon height (i.e., vertically projected length of stay cables), were also studied. Finally, the feasibility of the proposed stay cables was conceptually verified. It was shown that the proposed stay cables could be an excellent alternative to the pure CFRP or traditional steel stay cables.

Electrochemical Immunosensor for Carcinoembryonic Antigen Detection Based on Mo-Mn3O4/MWCNTs/Chits Nanocomposite Modified ITO Electrode

Wen-Ting Li,Yu Wang,Fen-Fang Deng,Li-Li Liu,Hai-Jun Nan,He Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

A novel electrochemical immunosensor for determination of carcinoembryonic antigen (CEA) in human serum was fabricated by depositing Mo–Mn3O4/MWCNTs/Chits nanocomposite onto an indium-tin oxide (ITO) electrode. Mo-doped Mn3O4 (MMO) was synthesized by sol–gel method and the presence of molybdenum improved its electrochemical properties. The MMO/ MWCNTs/Chits nanocomposite could accelerate the electron transfer rate and enlarge the surface area to capture a large number of Carcinoembryonic Antigen (CEA). The factors influencing the performance of the immunosensor were investigated, such as incubation time, incubation temperature and pH. Under optimal conditions, the electrochemical immunosensor could detect CEA in a linear range from 0.1 ng · mL-1 to 125 ng · mL-1 with a detection limit of 4.9 pg · mL-1 (S/N = 3). In addition, it exhibited high sensitivity and acceptable stability on a promising immobilization platform for signal amplification, which could be extended to other labeled recognition systems. This electrochemical immunosensor may provide potential applications for the clinical diagnosis.

Lateral-torsional Buckling Analysis of Cantilever Beam with Tip Lateral Elastic Brace under Uniform and Concentrated Load

Wen-Fu Zhang,Ying-Chun Liu,Gui-Lan Hou,Ke-Shan Chen,Jing Ji,Yun Deng,Shi-Lin Deng 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.4

The studies on the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace are rarely reported. The total potential energy is first established for the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace under uniform and concentrated load. The modal trial function of the lateral displacement and torsional angle are expressed as trigonometric function combination with six terms. By introducing new dimensionless parameters, the analytical solution of the dimensionless buckling equation for the lateral-torsional buckling of cantilever steel beam with tip lateral elastic brace is obtained. With the help of 1stOpt software which is mathematical optimization analysis software, the non-dimensional critical moment formula of the lateral-torsional buckling of cantilever beam with tip lateral elastic brace is obtained. Then, the accuracy of the formula is verified by ADINA finite element software. The results show that the given critical moment formula is of high accuracy. It provides convenient and simple design method for practical engineering.

Improving Bricks (kaolin) Performance by adding Incinerator Bottom Ash and Adjusting Kiln Calcination Temperatures

( Deng-fong Lin ),( Huan-lin Luo ),( Wei-zhu Wang ),( Jia-wen Chen ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-

The bottom ash (BA) is the residuals of the municipal trashes after incinerated in an incinerator plant. The BA is usually collected and landfilled as the final disposal treatment. However, it becomes harder finding locations for Taiwan as an island with a very scarce land for the landfill treatment. Moreover, the amount of the BA recycled was far behind the amount produced in which leading to a hard problem for central and local governments. How to properly recycle or reuse of the BA becomes an urgent need. From the results obtained by many different researchers, the use of the BA as a ceramic material may help improve the strength of related products. In this study, the BA was applied as part of the replacement for kaolin in the manufacture of the brick specimens. To investigate the effects of the kiln temperature on the brick specimens and evaluate the related properties of the specimens, the kiln temperatures were set at 850, 900, and 950℃. The municipal solid waste incineration BA was applied as part of kaolin replacement in the manufacture of bricks. Test results showed that the shrinkage increment of bricks tended to be smooth when the BA replacement was larger than 10%. Moreover, the results of loss on ignition indicated that the organic and inorganic matters and heavy metal were mostly burned off at the kiln temperature of 900℃ or above. The compressive strengths of bricks increased with the increase amount of BA replacement. The study of the micro structure observations showed that the silicification of the brick specimen bodies reduced with the increase amount of the BA replacement. Moreover, the results obtained from XRD indicated that the peak intensities of the SiO₂ in the brick specimens decreased with increase amount of the BA replacement. This study suggested that the optimal amount of BA replacement and the kiln temperature were 20% and 950℃, respectively.

Roles of E-Cadherin (CDH1) Genetic Variations in Cancer Risk: a Meta-analysis

Deng, Qi-Wen,He, Bang-Shun,Pan, Yu-Qin,Sun, Hui-Ling,Xu, Ye-Qiong,Gao, Tian-Yi,Li, Rui,Song, Guo-Qi,Wang, Shu-Kui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

E-Cadherin (CDH1) genetic variations may be involved in invasion and metastasis of various cancers by altering gene transcriptional activity of epithelial cells. However, published studies on the association of CDH1 gene polymorphisms and cancer risk remain contradictory, owing to differences in living habits and genetic backgrounds. To derive a more better and comprehensive conclusion, the present meta-analysis was performed including 57 eligible studies of the association between polymorphisms of CDH1 gene promoter -160 C>A, -347 G>GA and 3'-UTR +54 C>T and cancer risk. Results showed that these three polymorphisms of CDH1 were significantly associated with cancer risk. For -160 C>A polymorphism, -160A allele carriers (CA and CA+AA) had an increased risk of cancer compared with the homozygotes (CC), and the similar result was discovered for the -160A allele in the overall analyses. In the subgroup analyses, obvious elevated risk was found with -160A allele carriers (AA, CA, CA+AA and A allele) for prostate cancer, while a decreased colorectal cancer risk was shown with the AA genotype. For the -347 G>GA polymorphism, the GAGA genotype was associated with increased cancer risk in the overall analysis with homozygous and recessive models. In addition, results of subgroup analysis indicated that the elevated risks were observed in colorectal cancer and Asian descendants. For +54 C>T polymorphism, a decreased risk of cancer was found in heterozygous, dominant and allele models. Moreover, +54T allele carriers (CT, CT+TT genotype and T allele) showed a potential protective factor in gastric cancer and Asian descendants.