배양액내 아미노산 첨가가 돼지 난포란의 체외발달에 미치는 영향

위갑인,이경호,김은구,문승주,김재홍 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

체외성숙 배양액내에 첨가된 각각의 필수, 비필수, 필수 및 비필수아미노산이 돼지 미성숙난포란의 체외수정 또는 처녀발생유기후 초기배 발달에 미치는 영향에 대해서 검토한 결과는 다음과 같다. 1. 체외 배양 후 돼지수정란의 초기배 발달에 있어 체외 배양 후 48시간에서 대조구에 비해 각각의 필수아미노산 또는 비필수아미노산의 첨가시 4-8cell 발달율에서 유의적으로 높게 나타났고 (21.5%, 13.2% vs 6.9%, P<0.05), 72시간에서는 필수, 비필수, 필수 및 비필수아미노산 첨가시 초기배 발달율에서 유의적으로 높게 나타났다(49.2%, 48.9%,57.1%, vs 39.7%, P<0.05). 2. 처녀발생유기후 초기배 발달에 있어서는 대조구에 비해 배양 후 48시간에서 각각 필수아미노산과 비필수아미노산 첨가시 4-8cell단계까지의 발달율에서 통계적 유의차를 나타났고(15.2%, 19.3% vs 8.4%, P<0.05), 배양 후 72시간에서는 비필수아미노산의 첨가구가 4-8cell 발달율에서 대조구에 비해 유의적으로 높게 나타났다(25.5% vs 20.5% P<0.05). 이상의 결과로 미루어 볼때 돼지 난포란의 체외성숙 배양액내 첨가된 필수 및 비필수 아미노산이 난자의 성숙중에 축적되어 수정후 또는 처녀발생유기후 초기배의 발달에 필요한 단백질 합성에 영향을 주는 것으로 사려된다. The effect of amino acids supplemented to maturation medium on early embryo development of porcine oocytes matured and fertilized or chemically activated in vitro was studied. Porcine oocytes were matured in BSA-free Whitten's medium containing 10% porcine follicular fluid and 10% Eaa, Naa or Eaa+Naa with or without hormonal supplements for 20h. After maturation, the oocytes wee fertilized and chemically activated, and then cultured in CZB medium containing 0.4% BSA. After 48h of fertilization, supplementation of Eaa and Naa increased(P<0.05) 4-8cell developmental rate. After 72h of fertilization, supplementation of Eaa Naa and Eaa+Naa increased(P<0.05) total early embryo developmental rate. After 48h of chemical activation, supplementation of Eaa and Naa increased(P<0.05) 4-8cell developmental rate. After 72h of chemical activation, supplementation of Naa increased(P<0.05) 4-8cell developmental rate. In conclusion, suplementation of maturation medium with essential and non-essential amino acid improved early embryo development. These results suggest that essential and non-essential amino acids are accumulated during oocytes maturation and it increased protein synthesis which is essential to the early development of embryos.

허혈성심질환 환자에 있어 혈액 응고상태의 비교

이미경,송경은,이원길,김재식,박의현,전재은 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

연구배경: 최근에 지혈응고 및 섬유소 용해기능의 손상이 허혈성 심질환의 병인론에 중요한 역할을 한다고 알려져 왔다. 이에 저자들은 허혈성심질환군과 정상대조군에서 PT, aPTT, 섬유소원, α2-antiplasimin, tPA, PAI-I, AT Ⅲ, protein C, XL-FDP를 측정하여, 이들이 허혈성심질환의 발병에 미치는 영향 및 중요성에 연구하고, 혈전용해제 투여전과 투여후의 결과를 비교 분석하여 이들의 검사지표로서의 임상적인 유용성에 관하여 알아보고자 하였다. 방법: 39예의 정상대조군과 급성 심근경색 및 협심증으로 진단된 40예의 환자군에서 platelet-poor-plasma를 얻어 PT, aPTT, 섬유소원은 ELECTRA 1000C(Medical laboratory automation, Inc., USA)를 이용하여 측정하였고, α2-antiplasmin는 색소법으로 SPECT-ROLYSER α2-antiplasmin(Biopool AB, Canada)kit를, tPA와 PAI-1은 면역효소법으로, Protein C는 응고법으로 Bioclot?? Protein C(Biopool AB, Canada) kit를 사용하여 측정하였다. XL-FDP는 라텍스응집법으로 DIMERTESTR Ⅱ Latex Kit(AGEN Biomedical Limited, Australia)를 이용하여 측정하였다. 결과: 환자군과 정상대조군의 비교에서 에서 PT, aPTT, 섬유소원, PAI-1 및 ATⅢ는 두 군간에 통계학적으로 유의한 차의를 나타내었고 환자군에서는 62.5%에 해당하는 25예에서 증가된 소견을 보여 주었다. 환자군에서 혈전용해제 투여전과 투여후의 결과비교에서 PT와 섬유소원에서 유의한 차이가 있었으며, α2-antiplasmin, PAI-1 및 protein C는 유의하지는 않았지만 감소하는 경향을 나타내었다. 혈전용해제 투여후 1 시간결과를 대조군과 비교해 볼 때 PT와 tPA는 유의하게 증가하였고 섬유소원과 ATⅢ는 의의있게 감소하였다. 7일째 결과는 모든 지수에서 정상대조군과 유의한 차이가 없었다. 결론: PT, 섬유소원의 검사가 허혈성 심질환의 진단과 경과관찰에 도움을 줄 수 있으며, tPA, PAI-1, ATⅢ등과 같이 관찰함으로서 진단뿐만 아니라 예후와 재발위험성에 대한 예측 등 임상적으로 중요한 검사지표로서의 가능성을 나타냄을 알 수 있었다. Background: Many studies have shown that thrombus formation has important role in the pathogenesis of ischemic heart disease(IHD) and impaired fibrinolysis is associated with an increased risk of myocardial infacrtion. Forty patients with IHD and 39 normal controls were studied about blood coagulation, fibrinolytic, inhibitory proteins to evaluate their usefulness in diagnosing, predicting prognosis and monitoring after thrombolytic therapy in IHD. Method: Platelet-poor-plasma were obtained from the 3.8% trisodium citrate treated blood and prothrombin time(PT), activated partial thromboplastin time(aPTT), firbrinogen, α2-antiplasmin, tPA,PAI-1, antithrombin Ⅲ(ATⅢ), protein C. crosslinked fibrin degradation products(XL-FDP) were measured by appropriate functional or immunological assays. Results: The IHD group showed significant increases in PT,aPTT, fibrinogen, PAI-I and XL-FDP and significant reduction in AT Ⅲ concentration. No significant changes were observed between myocardial infarction and angina pectoris subgroups in the IHD. After thrombolytic therapy, PT and fibrinogen showed significant changes. In comparsion with normal controls, post 1 hour results showed significant changes in PT, fibrinogen, tPA, ATⅢ but no significant changes in post 7 days results. Conclusion: Our findings suggest that measurements of PT and fibrinogen are helpful for diagnosis in patients with IHD. And combined with tPA, PAI-1 and AT Ⅲ, they may be useful test for monitoring after thrombolytic therapy, predicting prognosis and risk of recurrence in IHD patients.

Identification of Anticoagulant Components in Korean Red Ginseng

Jae Joon Wee,Young Sook Kim,Jong Soo Kyung,Yong Bum Song,Jae Ho Do,Dong Chung Kim,Sung Dong Lee 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.4

In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extraction were further extracted with water and then dialyzed to obtain low and high molecular weight fractions. The anticoagulant activities of the seven fractions were evaluated in terms of thrombin time, prothrombin time, and activated partial thromboplastin time. Among these fractions, the ethylacetate fraction showed the most potent anticoagulant activity. The active components in the ethylacetate fraction were identified as the phenolic compounds vanillic, caffeic, ferulic, and p-coumaric acid via TLC and HPLC. These findings suggest that the anticoagulant activities of phenolic compounds contribute to the cardiovascular effects of KRG.

Antifuse Circuits and Their Applicatoins to Post-Package of DRAMs

Jae-Kyung Wee,Jeong-Hoon Kook,Se-Jun Kim,Sang-Hoon Hong,Jin-Hong Ahn 대한전자공학회 2001 Journal of semiconductor technology and science Vol.1 No.4

Compact DVB-H Antenna With Broad Dual-Band Operation for PMP Applications

Jae-Kyung Wee,Jae Woo Park,In Su Yeom,Boo-Gyoun Kim,Chang Won Jung IEEE 2010 IEEE antennas and wireless propagation letters Vol.9 No.-

<P>A dual-band (UHF: 470-862 MHz; L: 1452-1492 MHz) Digital Video Broadcasting-Handheld (DVB-H) antenna is presented. The proposed antenna is composed of a planar inverted-F antenna (PIFA) with an input impedance matching circuit. The matching circuit improves antenna performance in the broad UHF bands (470-862 MHz: 63%). The proposed antenna has omnidirectional patterns and sufficient gain (average peak gain over 470-862 MHz: 0.9 dBi) for the portable media player (PMP) applications. The antenna is contact with a PMP case (ε<SUB>r</SUB> = 3.2), which is used as a substrate for the size reduction and compact design.</P>

Antifuse Circuits and Their Applicatoins to Post-Package of DRAMs

Wee, Jae-Kyung,Kook, Jeong-Hoon,Kim, Se-Jun,Hong, Sang-Hoon,Ahn, Jin-Hong The Institute of Electronics and Information Engin 2001 Journal of semiconductor technology and science Vol.1 No.4

Several methods for improving device yields and characteristics have been studied by IC manufacturers, as the options for programming components become diversified through the introduction of novel processes. Especially, the sequential repair steps on wafer level and package level are essentially required in DRAMs to improve the yield. Several repair methods for DRAMs are reviewed in this paper. They include the optical methods (laser-fuse, laser-antifuse) and the electrical methods (electrical-fuse, ONO-antifuse). Theses methods can also be categorized into the wafer-level(on wafer) and the package-level(post-package) repair methods. Although the wafer-level laser-fuse repair method is the most widely used up to now, the package-level antifuse repair method is becoming an essential auxiliary technique for its advantage in terms of cost and design efficiency. The advantages of the package-level antifuse method are discussed in this paper with the measured data of manufactured devices. With devices based on several processes, it was verified that the antifuse repair method can improve the net yield by more than 2%~3%. Finally, as an illustration of the usefulness of the package-level antifuse repair method, the repair method was applied to the replica delay circuit of DLL to get the decrease of clock skew from 55ps to 9ps.

Anticoagulant activity of ethylacetate fraction of Korean Red Ginseng and its active components

Jae Joon Wee(위재준),Young Sook Kim,Jong Soo Kyung,Yong Bum Song,Jae Ho Do,Sung Dong Lee 고려인삼학회 2008 고려인삼학회 학술대회 Vol.2008 No.12

Identification of Anticoagulant Components in Korean Red Ginseng

Wee, Jae-Joon,Kim, Young-Sook,Kyung, Jong-Soo,Song, Yong-Bum,Do, Jae-Ho,Kim, Dong-Chung,Lee, Sung-Dong The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.4

In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extraction were further extracted with water and then dialyzed to obtain low and high molecular weight fractions. The anticoagulant activities of the seven fractions were evaluated in terms of thrombin time, prothrombin time, and activated partial thromboplastin time. Among these fractions, the ethylacetate fraction showed the most potent anticoagulant activity. The active components in the ethylacetate fraction were identified as the phenolic compounds vanillic, caffeic, ferulic, and p-coumaric acid via TLC and HPLC. These findings suggest that the anticoagulant activities of phenolic compounds contribute to the cardiovascular effects of KRG.

큰느타리버섯(Pleurotus eryngii) 조다당체 분획의 항산화 및 항종양활성

김재용(Jae-Yong Kim),강혜인(Hye-In Kang),박경욱(Kyung-Uk Park),문광덕(Kwang-Deog Moon),이상대(Sang-Dae Lee),조숙현(Sook-Hyun Cho),위재준(Jae-Joon Wee),경종수(Jong-Soo Kyung),송용범(Yong-Beom Song),서권일(Kwon-Il Seo) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.10

큰느타리 버섯으로부터 추출한 조다당체의 항산화 및 항암효과를 조사하였다. 항산화효과에 대한 실험에서 10, 100, 1,000 ㎍/mL 농도로 다당체의 처리시 linoleic acid에 대하여는 1,000 ㎍/mL 농도에서만 과산화물의 생성을 억제하였으나, 흰쥐의 microsome에 대하여는 각 농도에서 대조구에 비하여 5.3%, 9.4% 및 33%의 억제율을 나타내었다. 큰느타리버섯 다당체를 A549, MCF-7 및 AGS 암세포주에 같은 농도로 72시간 처리시 1,000 ㎍/mL 농도에서 대조구에 비하여 42.3, 33.4 및 26.7%의 증식억제 효과를 보였다. Sarcoma-180으로 복수암을 유발한 ICR mouse에 큰느타리버섯 다당체를 100 ㎎/㎏/day 및 300 ㎎/㎏/day의 농도로 7일간 처리하고 30일이 경과한 후 관찰한 결과 대조구에 비하여 각 농도에서 70% 및 90%의 수명연장 효과를 나타내었으며, 체중의 증가율도 대조구에 비하여 현저히 억제되었다. The aims of this study were to investigate the antioxidative and antitumor effects of crude polysaccharide fraction from Pleurtus eryngii (CPPE). CPPE inhibited autoxidation of linoleic acid at 1,000 ㎍/mL concentration, and the inhibitory rates of lipid peroxidation in rat liver microsome were 5.28, 9.40, and 32.5% at 10, 100, and 1,000 ㎍/mL concentrations, respectively. After treatment with CPPE for 72 hours, the inhibitory rates against MCF-7, A549 and AGS cell lines showed 42.3, 33.4 and 26.7% at concentration of 1,000 ㎍/mL, respectively. Results of CPPE treatment at 100 and 300 mg/kg/day for 7 days in sarcoma-180 bearing-mice showed survival rates of 70 and 90%, respectively. Body weights of mice treated with CPPE were significantly decreased when compared with the control.

Effect of a Korean Red Ginseng and Crude Drug-Combined Preparations (RGCDPs) on Memory Enhancement in Mice

Jae Joon Wee,Jong Soo Kyung,Na Mi Kim,Yong-Bum Song,Yi-Seong Kwak,Jong Dae Park 한국생약학회 2005 Natural Product Sciences Vol.11 No.4

Anti-amnestic activities of Korean red ginseng (Ginseng Radix Rubra) and crude drug-combined preparations (RGCDP-1, RGCDP-2, and RGCDP-3) were evaluated by the animal experiment. RGCDP-1 and RGCDP-2 were prepared based on Korean folk prescriptions, “Chongmyongtang” and Guibitang”, respectively,while RGCDP-3, by a combination of both. Among the three preparations, RGCDP-3 was found to show the most potent anti-amnestic activity as evaluated by the passive avoidance test with mice, indicating synergistic action by combined effects of RGCDP-1 and RGCDP-2.