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        • Poster Presentation : 2P-517 ; Structural and electrochemical characterization of proton beam irradiated manganese dioxide

          이원길,( C. Justin Raj ),유국현 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0

          Among various pseudo capacitive materials, manganese dioxide (MnO2) is a promising electrode material because of its high theoretical specific capacitance, abundant resources, low-cost, and eco-friendliness. But the poor conductivity and less electron transport properties of MnO2 limited their practical applications. The high power proton irradiation can effectively modify the electronic properties and surface states of the materials, also the irradiation can caused defects in the materials. Impurities in a crystal constitute structural imperfections which can alter electrical and mechanical properties of the materials. Moreover the irradiation creates secondary-electron charge carriers, and thus affects electrical properties of the materials, even it can improve the conductivity of the materials. We researched about electronic properties of the proton irradiated MnO2 by fabricating electrodes for super capacitor and checked the modifications of MnO2 nanostructure.

        • KCI등재

          인간 연골암세포주 HTB-94에서 Sulforaphane에 의한 PI-3K/AKT 경로를 통한 세포분화 조절 연구

          이원길,김송자 대한암예방학회 2013 Journal of cancer prevention Vol.18 No.1

          Sulforaphane (SFN) is an isothiocyanate derived from cruciferous vegetables such as broccoli, cauliflower, cabbage and kale. In previously study demonstrated that SFN regulated differentiation via disruption of actin cytoskeleton architecture in human chondrosarcoma, HTB-94 cells. In this study, we observed the molecular mechanism of SFN treatment on differentiation in human chondrosarcoma, HTB-94. We found that SFN increased the expression of type II collagen, SOX-9 and phosphorylation of AKT as determined by Western blot analysis. Also, SFN induced sulfated proteoglycan as determined by Alcian blue staining. Inhibition of PI-3K with LY294002 and Wortmannin decreased type II collagen expression. Also, Inhibition of AKT with MK2206 decreased type II collagen expression. However, activation of PI-3K with 740Y-P induced differentiation as determined by Western blot analysis and Alcian blue staining. These findings indicate that SFN regulates differentiation via PI-3K/AKT pathway in human chondrosarcoma, HTB-94 cells.

        • 적응신호 검출을 위한 간역화된 QNAR-AGC 시스템에 관한 연구

          이원길,박규호,박송배,Lee, Won-Gil,Park, Gyu-Ho,Park, Song-Bae 대한전자공학회 1985 전자공학회지 Vol.22 No.5

          자동이득 제어장치에서 신호대잡음비가 큰 신호나 중첩신호(ova.lapped signal)인 경우, 장치의 출력단에서 신호 감쇄가 일어나게 된다. 이런 문제점을 해결하기 위해 guasi-noise alone reference (QNAR)알고리즘이 발표 되었다. 그러나 이 QNAR 알고리즘을 계산의 복잡성 때문에 real time processing 이 어려운 단점을 가지고 있다. 본 논문에서는 이 문제점을 해결 하기위해 새로운 방법 즉 QNAR-Bl알고리즘을 제시 하였다. 제시된 QNAR-Bl 알고리즘은 계산의 복잡성을 대폭 감소시키면서도, 시abf레이션 결과 성능은 기존의 QNAR 알고리즘 보다 향상 되었다. This paper presents a simple method for drastic reduction of computational requirement for realization of the quasi.noise alone reference (QNAR) estimation of noise power. (1 ) The new estimator docs not bring forth performance degradation, as shown by extensive computer simulations, and is amenable to hardware implementation for certain real-time signal processing problems.

        • 최근 病院可檢物에서 분리되는 중요 細菌의 抗生劑感受性

          李源吉,金在崇,金重明,金在植 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

          1981년 본 대학 부속병원 임상병리 細菌檢査室에 의뢰된 검체에서 분리된 35種의 총 菌株수는 891株이었으며 소변에서 분리된 것이 301株(33.8%)로 가장 많았고 다음은 喀痰, 膿 및 咽喉순이었다. 100株 이상씩 분리된 菌種은 S. epidermidis, Micrococcus, S. aureus와 E. coli 순이었고 S. epidermidis는 小便과 喀痰, Micrococcus는 喀痰에서 S.aureus는 膿과 小便에서 E. coli는 小便에서 분리된 것이 대부분이었다. Streptococcus는 α-hemolyticus가 99株로서 喀痰에서 주로 분리되었고 50株 내외로 분리된 K. pneumoniae, Ent.cloacae, Ps. fluorescens, Citrobacter freundii와 Acinetobacter calcoaceticus는 각각 小便과 喀痰, 小便과 膿, 膿과 小便, 小便 및 小便에서 대부분 분리되었고 기타 菌種은 소수에서 분리되었다. 7種의 중요 菌種으로 부터 각각 무작위로 선택한 총 258株를 대상으로 하여 9種의 抗生劑에 대하여 disk diffusion(DD)와 agar dilution(AD)법으로 感受性을 조사하였다. E. coli 는 amikacin(AMK)에 매우 높은 感受性을 gentamycin(GAM)과 tobramycin(TOB)에 비교적 높은 感受性을 보였고 ampicillin(AMP), chloramphenicol(CAM) 및 tetracyaline(TET)에 비교적 높은 耐性을 나타내었고 K. pneumoniae는 AMK에 높은 感受性을 CEP, GAM 및 TOB에 비교적 높은 感受性을 보였고 AMP에 고도의 耐性, CAM과 TET에는 비교적 높은 感受性을 CAM과 GAM에는 중등도의 感受性을 penicillin과 TET에 고도의 耐性을 보였다. Ser. marces-cens 는 AMK에 중등도의 感受性을 GAM에 낮은 感受性을 보이나 나머지 抗生劑에 고도의 耐性을 보였다. Ps. aeruginosa는 AMK에 비교적 높은 感受性을 GAM, TOB 및 carbenicillin에 비교적 낮은 感受性을 보였고 Salmonella group A는 AMK, GAM, AMP, CEP, CAM 및 TOB에 매우 높은 感受性의 경향을 나타내었고 TET에도 비교적 높은 感受性의 경향을 나타내었다. Shigella group B는 AMK CEP, GAM 및 TOB에 매우 높은 感受性의 경향을 그리고 AMP, CAM 및 TET에 매우 높은 耐性의 경향을 보였다. Amikacin이 모든 供試菌들에 대하여 가장 强한 抗菌力을 보였고 Serratia 菌種과 Pseudomonas菌種은 다른 供試菌種에 비하여 확실히 耐性의 傾向을 나타내었다. Agar dilution 法과 disk diffusion 法에서 이 두방법은 서로 잘 일치하는 경향이나 DD법이 AD법에 비하여 안정성을 보이는 것 같았다. 검체에 의한 각 菌種사이에 있어서 感受性의 차이는 볼 수 없었다. Total numbers of isolated bacteria were 35 species and were 891 strains. The highest isolates were 301 strains (33.8%) which were from urine specimens and followed by sputum, pus and throat. S. epidermidis, Micrococcus, S. aureus and E. coli in order were over 100 strains. S.epidermidis strains were isolated mainly from urine and sputum, Micrococcus strains from sputum, S. aureus strains from pus, and urine and E. coli from urine, and Streptococcus (α-hemolyticus) were 99 strains which were isolated from mainly sputum. K. Preumoniae, Ent. cloacae, Ps. fluorescens, Citrobacter freundii and Acinetobacter calcoaceticus were more or less 50 strains and isolated mainly from urine and sputum, urine and pus, and urine, urine and urine respectively. Others were in small numbers. Totally 258 strains selected at random from 7 important bacteriae which were selected recently and antibiotic susceptibility testings to these orgamisms were performed. E. coli was very sensitive to amikacin (AMK), moderately sensitive to gentamycin (GAM) tobramycin (TOB) and moderately risistant to ampicillin (AMP), chloramphenicol (CAM) and tetraciclin (TET). K. pneumoniae was highly sensitive to AMK, moderately sensitive to cephalothin (CEP), gentamycin (GAM) and TOB, very highly resistantce to ampicillin (AMP) and highly resistant to CAM and TET. S. aureus was very sensitive to AMK, and CEP, moderately sensitive to CAM and GAM, and very highly resistant to penicillin and TET. Ser. marcescens revealed low sensitivity to AMK and GAM but resistances to the other antibiotics. Salmonella group A showed very high sensitivity to AMK, GAM, AMP, CEP, CAM and TOB and high sensitivity even in TET. Shigella group B were very highly sensitive to AMK, CEP, GAM and TOB, but very highly resistant to AMP, CAM and TET. AMK showed the highest sensititity to the most organism but Serratia and Pseudomonas revealed distinctively high resistances to antibiotics even in the new potent antibiotics of broad spectrum. The disk diffusion and agar dilution method were generally corresponding but the former appeared to be more reliable. There were no remarkable difference in susceptibility from specimen to specimen.

        • KCI등재

          Increase of proliferation and DNA damage in mouse hepatocytes treated with diethylnitrosamine

          이원길,강진석 충북대학교 동물의학연구소 2017 Journal of Biomedical and Translational Research Vol.18 No.2

          As diethylnitrosamine (DEN) effect on cell proliferation, DNA damage and stem cell marker(s) expression have been largely unknown in mouse normal hepatocytes (AML-12 cells) cultured over a short-term period, this study was conducted to examine the cell proliferation, Ataxia telangiectasia mutated (ATM) and epithelial cell adhesion molecule (EpCAM) and Neighbor of Punc E 11 (Nope) expression in AML-12 cells treated with DEN for 24 and 48 h. Cells were treated with DEN (25-800 μg/mL) and cell phenotype was determined, and the MTT assay was used to quantify the proliferation of cells treated with DEN. Expression and distribution of ATM in AML-12 cells were determined by indirect immunofluorescence microscopy. And Western blot analysis of EpCAM and Nope was performed. Cell viability was significantly increased in response to all doses of DEN treatment compared to control at 24 h (p<0.05 or p<0.01). However, there was no significant increase at 48 h, even though it showed increased trend. Immunofluorescence staining of ATM showed that there was an increase of ATM expression at doses of 50, 100 and 200 μg/mL of DEN treatment, showing strong nuclear staining. Furthermore, Western blot analysis showed that DEN treatment showed increased trend of EpCAM and Nope expression. Taken together, DEN treatment increased cell proliferation in AML-12 cells, and it was associated with increased ATM expression.

        • KCI등재

          Collagen-coated Polyethylene Terephthalate 디스크 Plate와 Shell Vial을 이용한 Chlamydophila pneumoniae 배양법의 비교

          이원길,김미혜 대한임상미생물학회 2010 Annals of clinical microbiology Vol.13 No.2

          배경: 호흡기 감염의 중요한 원인 중 하나인 Chlamydophila pneumoniae의 정확한 진단을 위해서는 세포 배양을 통해 C. pneumoniae의 검출을 확인하는 것이 중요하다. 최근 콜라겐이 코팅된 polyethylene terephthalate 디스크(PET 디스크)를polyester plate 용기에 넣어 사용하는 제품(CorePath, Daegu, Korea)이 개발되어 세포 배양에 이용되고 있다. 이에 저자는PET 디스크 plate의 유용성을 평가하고 기존의 방법과 비교하기 위해 본 연구를 실시하였다. 방법: C. pneumoniae 배양이 의뢰된 환자 46명에서 채취한 29개의 객담과 17개의 혈액 검체를 대상으로 shell vial과 PET 디스크 plate (4-well)를 이용하여 배양하였고 결과가 상이한 경우 microimmunofluorescence (MIF) 결과를 비교하였다. 결과: HeLa-229 세포단층은 모든 shell vial과 PET 디스크 plate에서 관찰되었으며, 양성인 경우 C. pneumoniae 배양 결과세포질 내에 하나의 둥근 봉입체가 형광 염색되었다. 두 검사법에서 판정이 일치하는 경우는 양성 20예와 음성 22예,총 42예로 91.3%의 우수한 일치율(k=0.826)을 보였다. 결론: PET 디스크 plate 배양법은 shell vial법의 결과와 비교하여 우수한 결과 일치율을 보였으며, 또한 plate와 디스크를다루는 데에도 어려움이 없어 shell vial의 cover glass를 대신하여 PET 디스크 plate를 사용하여도 좋을 것으로 판단된다. [대한임상미생물학회지 2010;13:73-78] Background: Chlamydophila pneumoniae is one of the major respiratory infectious pathogens and can be accurately diagnosed by cell culturing. The author performed this study to compare the usefulness of the collagen-coated polyethylene terephthalate (PET)disc culture method and that of the shell vial method. Methods: Twenty-nine sputums and 17 blood specimens collected from 46 patients for C. pneumoniae culture were inoculated into HeLa-229 cell monolayers cultured in shell vials and polyester plates. After incubation, they were stained using the indirect immunofluorescent method with genus-specific FITC-conjugated anti-chlamydia antibody. When both results were inconsistent, microimmunofluorescence results were used. Results: HeLa-229 cells successfully formed monolayers in shell vials and collagen-coated PET plates in all cases. Positive inclusion bodies in HeLa-229cells of shell vials and PET plates for C. pneumoniae culture were similarly stained with the indirect immunofluorescent method. Both methods showed consistent results with 20 positive and 22 negative cases. The total agreement between the PET plate and shell vial was excellent (91.3%, k=0.826). Conclusion: The collagen-coated PET disc culture method showed highly consistent results with that of the shell vial method, and no technical differences were experienced between the two methods. Therefore, the author concluded that the shell vial method could be replaced by the PET plate method for detection of C. pneumoniae. (Korean J Clin Microbiol 2010;13:73-78)

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