fMRI를 이용한 성인 편마비의 항조절점 운동이 대뇌피질의 활성화에 미치는 효과

이원길,Lee Won-Kil 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.3

This study investigated activation of cerebral cortex in patients with hemiplegia that was caused by neural damage. Key-point control movement therapy of Bobath was performed for 9 weeks in 3 subjects with hemiplegia and fMRI was used to compare and analyze activated degree of cerebral cortex in these subjects. fMRI was conducted using the blood oxygen level-dependent(BOLD) technique at 3.0T MR scanner with a standard head coil. The motor activation task consisted of finger flexion-extension exercise in six cycles(one half-cycles = 8 scans = $3\;sec{\times}\;8\;=\;24\;sec$). Subjects performed this task according to visual stimulus that sign of right hand or left hand twinkled(500ms on, 500ms off). After mapping activation of cerebral motor cortex on hand motor function, below results were obtained. 1. Activation decreased in primary motor area, whereas it increased in supplementary motor area and visual association area(p<.001). 2. Activation was observed in bilateral medial frontal gyrus, middle frontal gyrus of left cerebrum, inferior frontal gyrus, inter-hemispheric, fusiform gyrus of right cerebrum, superior parietal lobule of parietal lobe and precuneus in subjedt 1, parahippocampal gyrus of limbic lobe and cingulate gyrus in subject 2, and inferior frontal gyrus of right frontal lobe, middle frontal gyrus, and inferior parietal lobule of left cerebrum in subject 3 (p<.001). 3. Activation cluster extended in declive of right cellebellum posterior lobe in subject 1, culmen of anterior lobe and declive of posterior lobe in subject 2, and dentate gyrus of anterior lobe, culmen and tuber of posterior lobe in subject 3 (p<.001). In conclusion, these data showed that Key-point control movement therapy of Bobath after stroke affect cerebral cortex activation by increasing efficiency of cortical networks. Therefore mapping of brain neural network activation is useful for plasticity and reorganization of cerebral cortex and cortico-spinal tract of motor recovery mechanisms after stroke.

현대전자 통신전자 시험평가의 중요성

이원길,Lee, Won-Gil 한국방위산업진흥회 1997 國防과 技術 Vol.- No.224

전투에서의 승리를 위해 전자장비 및 이를 탑재한 무기체계들이 현대의 전장환경에서 성능저하 없이 정상적으로 운용될 수 있도록 장비가 설계 및 제작되어야 하며, 전장환경과 유사한 조건하에서 철저한 시험평가를 거쳐 전자정비의 신뢰성과 생존성이 보장되어야 한다.

인간 연골암세포주 HTB-94에서 Sulforaphane에 의한 PI-3K/AKT 경로를 통한 세포분화 조절 연구

이원길,김송자 대한암예방학회 2013 Journal of cancer prevention Vol.18 No.1

Sulforaphane (SFN) is an isothiocyanate derived from cruciferous vegetables such as broccoli, cauliflower, cabbage and kale. In previously study demonstrated that SFN regulated differentiation via disruption of actin cytoskeleton architecture in human chondrosarcoma, HTB-94 cells. In this study, we observed the molecular mechanism of SFN treatment on differentiation in human chondrosarcoma, HTB-94. We found that SFN increased the expression of type II collagen, SOX-9 and phosphorylation of AKT as determined by Western blot analysis. Also, SFN induced sulfated proteoglycan as determined by Alcian blue staining. Inhibition of PI-3K with LY294002 and Wortmannin decreased type II collagen expression. Also, Inhibition of AKT with MK2206 decreased type II collagen expression. However, activation of PI-3K with 740Y-P induced differentiation as determined by Western blot analysis and Alcian blue staining. These findings indicate that SFN regulates differentiation via PI-3K/AKT pathway in human chondrosarcoma, HTB-94 cells.

Modulation of Apoptosis and Differentiation by the Treatment of Sulfasalazine in Rabbit Articular Chondrocytes

이원길,강진석 한국독성학회 2016 Toxicological Research Vol.32 No.2

This study was conducted to examine the cellular regulatory mechanisms of sulfasalazine (SSZ) in rabbit articular chondrocytes treated with sodium nitroprusside (SNP). Cell phenotype was determined, and the MTT assay, Western blot analysis and immunofluorescence staining of type II collagen was performed in control, SNP-treated and SNP plus SSZ (50~200 μg/mL) rabbit articular chondrocytes. Cellular proliferation was decreased significantly in the SNP-treated group compared with that in the control (p < 0.01). SSZ treatment clearly increased the SNP-reduced proliferation levels in a concentration-dependent manner (p < 0.01). SNP treatment induced significant dedifferentiation and inflammation compared with control chondrocytes (p < 0.01). Type II collagen expression levels increased in a concentration-dependent manner in response to SSZ treatment but were unaltered in SNP-treated chondrocytes (p < 0.05 and < 0.01, respectively). Cylooxygenase-2 (COX-2) expression increased in a concentration-dependent manner in response to SSZ treatment but was unaltered in SNP-treated chondrocytes (p < 0.05). Immunofluorescence staining showed that SSZ treatment increased type II collagen expression compared with that in SNP-treated chondrocytes. Furthermore, phosphorylated extracellular regulated kinase (pERK) expression levels were decreased significantly in the SNP-treated group compared with those in control chondrocytes (p < 0.01). Expression levels of pERK increased in a concentration-dependent manner by SSZ but were unaltered in SNP-treated chondrocytes. pp38 kinase expression levels increased in a concentrationdependent manner by SSZ but were unaltered in control chondrocytes (p < 0.01). In summary, SSZ significantly inhibited nitric oxide-induced cell death and dedifferentiation, and regulated extracellular regulated kinases 1 and 2 and p38 kinase in rabbit articular chondrocytes.

세균배양용 배지의 정도관리

이원길 대한임상검사정도관리학회 1986 臨床檢査와 精度管理 Vol.8 No.2

[생태수필 연재] 예끼마을에 살면서 ③

이원길 수필미학 2023 수필미학 Vol.41 No.-

최근 病院可檢物에서 분리되는 중요 細菌의 抗生劑感受性

李源吉,金在崇,金重明,金在植 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

1981년 본 대학 부속병원 임상병리 細菌檢査室에 의뢰된 검체에서 분리된 35種의 총 菌株수는 891株이었으며 소변에서 분리된 것이 301株(33.8%)로 가장 많았고 다음은 喀痰, 膿 및 咽喉순이었다. 100株 이상씩 분리된 菌種은 S. epidermidis, Micrococcus, S. aureus와 E. coli 순이었고 S. epidermidis는 小便과 喀痰, Micrococcus는 喀痰에서 S.aureus는 膿과 小便에서 E. coli는 小便에서 분리된 것이 대부분이었다. Streptococcus는 α-hemolyticus가 99株로서 喀痰에서 주로 분리되었고 50株 내외로 분리된 K. pneumoniae, Ent.cloacae, Ps. fluorescens, Citrobacter freundii와 Acinetobacter calcoaceticus는 각각 小便과 喀痰, 小便과 膿, 膿과 小便, 小便 및 小便에서 대부분 분리되었고 기타 菌種은 소수에서 분리되었다. 7種의 중요 菌種으로 부터 각각 무작위로 선택한 총 258株를 대상으로 하여 9種의 抗生劑에 대하여 disk diffusion(DD)와 agar dilution(AD)법으로 感受性을 조사하였다. E. coli 는 amikacin(AMK)에 매우 높은 感受性을 gentamycin(GAM)과 tobramycin(TOB)에 비교적 높은 感受性을 보였고 ampicillin(AMP), chloramphenicol(CAM) 및 tetracyaline(TET)에 비교적 높은 耐性을 나타내었고 K. pneumoniae는 AMK에 높은 感受性을 CEP, GAM 및 TOB에 비교적 높은 感受性을 보였고 AMP에 고도의 耐性, CAM과 TET에는 비교적 높은 感受性을 CAM과 GAM에는 중등도의 感受性을 penicillin과 TET에 고도의 耐性을 보였다. Ser. marces-cens 는 AMK에 중등도의 感受性을 GAM에 낮은 感受性을 보이나 나머지 抗生劑에 고도의 耐性을 보였다. Ps. aeruginosa는 AMK에 비교적 높은 感受性을 GAM, TOB 및 carbenicillin에 비교적 낮은 感受性을 보였고 Salmonella group A는 AMK, GAM, AMP, CEP, CAM 및 TOB에 매우 높은 感受性의 경향을 나타내었고 TET에도 비교적 높은 感受性의 경향을 나타내었다. Shigella group B는 AMK CEP, GAM 및 TOB에 매우 높은 感受性의 경향을 그리고 AMP, CAM 및 TET에 매우 높은 耐性의 경향을 보였다. Amikacin이 모든 供試菌들에 대하여 가장 强한 抗菌力을 보였고 Serratia 菌種과 Pseudomonas菌種은 다른 供試菌種에 비하여 확실히 耐性의 傾向을 나타내었다. Agar dilution 法과 disk diffusion 法에서 이 두방법은 서로 잘 일치하는 경향이나 DD법이 AD법에 비하여 안정성을 보이는 것 같았다. 검체에 의한 각 菌種사이에 있어서 感受性의 차이는 볼 수 없었다. Total numbers of isolated bacteria were 35 species and were 891 strains. The highest isolates were 301 strains (33.8%) which were from urine specimens and followed by sputum, pus and throat. S. epidermidis, Micrococcus, S. aureus and E. coli in order were over 100 strains. S.epidermidis strains were isolated mainly from urine and sputum, Micrococcus strains from sputum, S. aureus strains from pus, and urine and E. coli from urine, and Streptococcus (α-hemolyticus) were 99 strains which were isolated from mainly sputum. K. Preumoniae, Ent. cloacae, Ps. fluorescens, Citrobacter freundii and Acinetobacter calcoaceticus were more or less 50 strains and isolated mainly from urine and sputum, urine and pus, and urine, urine and urine respectively. Others were in small numbers. Totally 258 strains selected at random from 7 important bacteriae which were selected recently and antibiotic susceptibility testings to these orgamisms were performed. E. coli was very sensitive to amikacin (AMK), moderately sensitive to gentamycin (GAM) tobramycin (TOB) and moderately risistant to ampicillin (AMP), chloramphenicol (CAM) and tetraciclin (TET). K. pneumoniae was highly sensitive to AMK, moderately sensitive to cephalothin (CEP), gentamycin (GAM) and TOB, very highly resistantce to ampicillin (AMP) and highly resistant to CAM and TET. S. aureus was very sensitive to AMK, and CEP, moderately sensitive to CAM and GAM, and very highly resistant to penicillin and TET. Ser. marcescens revealed low sensitivity to AMK and GAM but resistances to the other antibiotics. Salmonella group A showed very high sensitivity to AMK, GAM, AMP, CEP, CAM and TOB and high sensitivity even in TET. Shigella group B were very highly sensitive to AMK, CEP, GAM and TOB, but very highly resistant to AMP, CAM and TET. AMK showed the highest sensititity to the most organism but Serratia and Pseudomonas revealed distinctively high resistances to antibiotics even in the new potent antibiotics of broad spectrum. The disk diffusion and agar dilution method were generally corresponding but the former appeared to be more reliable. There were no remarkable difference in susceptibility from specimen to specimen.

몽동발이

이원길 수필미학 2015 수필미학 Vol.7 No.-

현대전과 통신전자 시험평가의 중요성(2)

이원길,Lee, Won-Gil 한국방위산업진흥회 1997 國防과 技術 Vol.- No.225

선진국은 현대전 및 미래전에서 통신/전자장비의 생존성이 전쟁승패의 주요 요소임을 인식하고 장비 획득규정 및 관련 군사규격을 보완하며, 취약성 시험과 EMP, EMR을 비롯한 각종 전자환경시험 능력을 강화하고 있으며, 첨단무기체계 개발에 따른 전자시험평가 능력을 지속적으로 발전시키고 있다. 하지만 우리의 경우 현대전에서 그 중요성이 부각되고 있는 취약성시험과 전자환경 시험평가 능력은 매우 미흡한 수준이다.

[생태수필 연재] 예끼마을에 살면서 4

이원길 수필미학 2023 수필미학 Vol.42 No.-