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무균돼지 사육시설 계획을 위한 사례연구 : 공간구성 및 동선계획을 중심으로
권순정,성제경,염수청 한국의료복지시설학회 2006 의료·복지 건축 Vol.12 No.1
According to the increase of demand for human organs such as kidney, heart, pancreas, joint, and cornea for therapeutic transplantation, the production of alternative organs based on Gnotobiotic Pigs gains a lot of concerns all over the world. However, it is not common to design and build Gnotobiotic Pigs' facility, and there are only a few those facilities and planning principles for them. Considering the situation above, this paper tries to develop planning guidelines for space organization and circulation system of standardized Germ Free Pig's facility on the bases of case analysis. The results of this study are as follows. At first, four swine farms including a Gntobiotic Pig's facility has been analysed from the point of space organization and circulation system. Secondly, the space zoning of Gnotobiotic Pigs' facility has been proposed into 5 groups : pigs' area, adminstration area, operating room and laboratory, service area, and mechanical area. Space components of each group have been presented also. Finally, circulation system of Gnotobiotic Pigs' facility has been explored from a operational point of view. This, also, includes human circulation, pig's circulation, and goods' circulation. This study has some limitations because it does not consider the SOPs(standard operational policies) of that facility to the fullest measure and does not suggest space area of each part, either. Despite of some weaknesses, it is expected that this study can give some useful guidelines for the design and planning of Germ Free Pigs' facilities.
CRISPR and SSTR mediated knock-in with mouse embryos
Su-Cheong Yeom 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knock-in animal generation via homology directed repair (HDR) is not as efficient as non-homologous end-joining DNA-repair-dependent knockout. Although its double-strand break activity may vary, Cas9 derived from Streptococcus pyogenens allows robust design of single-guide RNAs (sgRNAs) within the target sequence; However, prescreening for different sgRNA activities delays the process of transgenic animal generation. To overcome this limitation, multiple sets of different sgRNAs were examined for their knock-in efficiency. We discovered profound advantages associated with single-stranded oligo-donor-mediated HDR processes using overlapping sgRNAs (sharing at least five base pairs of the target sites) as compared with using non-overlapping sgRNAs for knock-in mouse generation. Studies utilizing cell lines revealed shorter sequence deletions near target mutations using overlapping sgRNAs as compared with those observed using non-overlapping sgRNAs, which may favor the HDR process. Using this simple method, we successfully generated several transgenic mouse lines harboring loxP insertions or single-nucleotide substitutions with a highly efficiency of 18~38%. Our results demonstrate a simple and efficient method for generating transgenic animals harboring foreign-sequence knock-ins or short-nucleotide substitutions by the use of overlapping sgRNAs.
YEOM, Su-Cheong,YU, Sun-A,CHOI, Eun-Young,LEE, Byeong-Chun,LEE, Wang-Jae Japanese Association for Laboratory Animal Science 2009 Experimental animals Vol.58 No.5
<P>We investigated the prevalence of <I>Helicobacter hepaticus</I>, murine norovirus (MNV), and <I>Pneumocystis carinii</I> and the efficacy of cross-fostering for their eradication in 49 genetically engineered mouse (GEM) strains at our institute. Prevalences of <I>H. hepaticus</I>, MNV, and <I>P. carinii</I> were 33.9, 36.5, and 8.6%, respectively, and immunodeficient strains showed relatively higher prevalence of the 3 pathogens than immunocompetent strains. Additionally, the same immune phenotype strains showed similar prevalences. Furthermore, it was found that NKT cells might play a role in <I>H. hepaticus</I> resistance. Interestingly, there was a high incidence of <I>H. hepaticus</I> and MNV multiple infection. Strains with single or multiple infections of <I>H. hepaticus</I>, MNV, and/or <I>P. carinii</I> were selected, and cross-fostering was conducted. Cross-fosterings were successful at eradicating <I>P. carinii</I>, but there were some failures for <I>H. hepaticus</I> and MNV, and the efficacy of eradication was relatively low compared with previous studies. We thought that this low efficacy might have been due to persistent infection and the high suscepibility to <I>H. hepaticus</I> and MNV of immunodeficient GEM strains. Therefore, cross-fostering may be appropriate for <I>P. carinii</I> eradication, but be inappropriate for repopulation of a new breeding colony with <I>H. hepaticus</I> or MNV infected GEM strains. Our findings provide basic data on maintenance, strain susceptibility, and successful rederivation, especially for GEMs.</P>
Native Void Space for Maximum Volumetric Capacity in Silicon-Based Anodes
Yeom, Su Jeong,Lee, Cheolmin,Kang, Sujin,Wi, Tae-Ung,Lee, Chanhee,Chae, Sujong,Cho, Jaephil,Shin, Dong Ok,Ryu, Jungki,Lee, Hyun-Wook American Chemical Society 2019 NANO LETTERS Vol.19 No.12
<P>Volumetric energy density is considered a primary factor in developing high-energy batteries. Despite its significance, less efforts have been devoted to its improvement. Silicon-based materials have emerged as next-generation anodes for lithium-ion batteries due to their high specific capacity. However, their volumetric capacities are limited by the volume expansion rate of silicon, which restricts mass loading in the electrodes. To address this challenge, we introduce porous silicon templated from earth-abundant minerals with native internal voids, capable of alleviating volumetric expansion during repeated cycles. In situ transmission electron microscopy analysis allows the precise determination of the expansion rate of silicon, thus presenting an analytical model for finding the optimal content in silicon/graphite composites. The inner pores in silicon reduce problems associated with its expansion and allow higher silicon loading of 42% beyond the conventional limitations of 13-14%. Consequently, the anode designed in this work can deliver a volumetric capacity of 978 mAh cc<SUP>-1</SUP>. Thus, suppressing volume expansion with natural abundant template-assisted materials opens new avenues for cost-effective fabrication of high volumetric capacity batteries.</P> [FIG OMISSION]</BR>