Mediated Moderation Analysis in the Nexus of Investment in Human Capital and the Green Economy

Lijiang Wang,UTAI UPRASEN,Qingmei Li 한국무역연구원 2023 무역연구 Vol.19 No.3

Purpose – The purpose of this paper is to analyze the mediated moderation role of foreign direct investment inflows (FDI), along with technological innovation (TI), in the relationship between investment in human capital (IHC) and the green economy, which is often ignored in current literature. Design/Methodology/Approach – In order to estimate the mediated moderating effect of TI and FDI on the nexus of IHC and the green economy, we use principle component analysis (PCA) to create a green economy index (GEI) by first selecting 15 relevant variables. Furtherly, the data analyzed covers 33 OECD nations from 2005 to 2018 using a Generalized Method of Moments (GMM) model. Findings – This paper demonstrates that while FDI positively moderates the relationship between IHC and the green economy, the positive role of FDI can be hindered by TI as a mediating variable in OECD countries. The empirical result reveals a U-shaped association between IHC and the green economy. Moreover, we also explain the joint effect of FDI and IHC on TI in OECD countries. FDI shows a negative role in the impact of IHC on TI. Research Implications – This paper puts forward dialectical thinking to view the impact of human capital investment on the green economy, and suggests that governments should view role of IHC in the green economy dialectically, plus adequately utilize its promotional role to address associated risks. The findings can support decision makers in how to use IHC precisely to achieve the goal of sustainable development.

Immobilization and Performance of Penicillin G Acylase on Magnetic Ni_0.7Co_0.3Fe₂O₄@SiO₂-CHO Nanocomposites

( Zhixiang Lv ),( Qingmei Yu ),( Zhou Wang ),( Ruijiang Liu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6

Magnetic Ni_0.7Co_0.3Fe₂O₄ nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic Ni_0.7Co_0.3Fe2O₄@SiO₂-CHO nanocomposites, on which penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and 45 ℃ while immobilized PGA’s a ctivities p eaked at p H 7.5 and 4 5 ℃. Immobilized PGA had better thermal stability than free PGA at the range of 30-50 ℃ for different time intervals. The activity of free PGA would be 0 and that of immobilized PGA still retained some activities at 60 ℃ after 2 h. V_max and K_m of immobilized PGA were 1.55 mol/min and 0.15 mol/l, respectively. Free PGA’s V_max and K_m separately were 0.74 mol/min and 0.028 mol/l. Immobilized PGA displayed more than 50% activity after 10 successive cycles. We concluded that immobilized PGA with magnetic Ni_0.7Co_0.3Fe₂O₄@SiO₂-CHO nanocomposites could become a novel example for the immobilization of other amidohydrolases.

Differences in the Transference of Humor and Personification in Advertisement Translation

Ying Cui,Qingmei Wang 세종대학교 언어연구소 2010 Journal of Universal Language Vol.11 No.2

This study analyses the transference of humor in translating advertising texts and, based on research in a prior study, proposes that the transference of humor is quite flexible in the practice of advertisement translation. The humorous expressions in original texts may be faithfully translated, or deleted, or in some cases humor may be added in translated texts. Compared with such flexible ways of dealing with humor, the translation of personify-cation in our corpus is relatively more faithful. In this paper, we are trying to figure out the implications behind the difference in terms of the treatment of humor and personification in advertisement translation. Similar to the former study, here we will also take the presupposition perspective. When the personification is reproduced faithfully in translations, it can be inferred that translators’ presuppositions about the target audience’s potential perception are the same with those held by the original writers about their targeted readers. This point in turn implies that the original and target cultures are correspondent in those aspects. Similarly, the unfaithful or flexible treatment of humor implies the opposite. In our research we mainly focus on translation between Chinese and English

Long non-coding RNA RP11-6O2.4 indicates poor prognosis and suppresses cell cycle progression through the p38-MAPK signaling pathway in gastric cancer

Yang Feng,Zhiming Fu,Yajun Luo,Wang Tan,Zilin Liu,Pengcheng Ye,Fei Lu,Wanping Xiang,Linghan Tang,Lin Yao,Mengyun Song,Qingmei Huang,Yilun Liu,Jiangwei Xiao 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3

Backgrounds: The role of long non-coding RNAs (lncRNA) in gastric cancer (GC) has been highlighted in studies conducted over the past decade. However, the potential clinical value and the mechanisms of action of RP11-6O2.4 in GC have not been thoroughly elucidated to date. The specific aim of the present study was to assess RP11-6O2.4 and to explore its role in human GC. Methods: Quantitative real-time polymerase chain reaction (qPCR) was performed to analyze the expression levels of RP11-6O2.4 in GC tissues, paired adjacent noncancerous tissues (ANTs) and GC cell lines. In addition, the correlation between RP11-6O2.4 expression and the clinical characteristics and prognosis of patients with GC was statistically analyzed. The effects of RP11- 6O2.4 on the GC cell cycle transformation through the p38-MAPK signaling pathway were explored by flow cytometry, qPCR and Western blot analysis after treatment with SB203580, a p38MAPK specific inhibitor, in vitro. Results: The expression levels of RP11-6O2.4 in GC tissues were significantly lower than the paired ANTs (P<0.05). In addition, RP11-6O2.4 expression was significantly lower in cases with older age, longer maximum tumor diameter, higher ASA grade and deeper invasive depth (P<0.05). RP11-6O2.4 expression was significantly higher in cases with well/middle differentiation than poor/no differentiation; higher in cases without lymph node metastasis than in lymph node metastasis; and higher in cases in stage Ⅰ/Ⅱ than in stage Ⅲ/Ⅳ. An in vitro assay showed that RP11-6O2.4 induced G0/ G1 phase cell cycle arrest, likely by regulating the p38- MAPK signaling pathway. Conclusion: The above mentioned data suggested that RP11-6O2.4 was a tumor-suppressor gene in GC. RP11- 6O2.4 might play an important role in the cell cycle transformation by regulating the p38-MAPK signaling pathway, thereby representing a specific biomarker and a potential molecular target for the treatment of GC.

Hair Growth Promoting Effects of 650 nm Red Light Stimulation on Human Hair Follicles and Study of Its Mechanisms via RNA Sequencing Transcriptome Analysis

( Kai Yang ),( Yulong Tang ),( Yanyun Ma ),( Qingmei Liu ),( Yan Huang ),( Yuting Zhang ),( Xiangguang Shi ),( Li Zhang ),( Yue Zhang ),( Ji’an Wang ),( Yifei Zhu ),( Wei Liu ),( Yimei Tan ),( Jinran 대한피부과학회 2021 Annals of Dermatology Vol.33 No.6

Background: Androgenetic alopecia (AGA) leads to thinning of scalp hair and affects 60%~70% of the adult population worldwide. Developing more effective treatments and studying its mechanism are of great significance. Previous clinical studies have revealed that hair growth is stimulated by 650-nm red light. Objective: This study aimed to explore the effect and mechanism of 650-nm red light on the treatment of AGA by using ex vivo hair follicle culture. Methods: Human hair follicles were obtained from hair transplant patients with AGA. Hair follicles were cultured in Williams E medium and treated with or without 650-nm red light. Real-time RT-PCR and immunofluorescence staining were used to detect the expression level of genes and proteins in hair follicles, respectively. RNA-sequencing analysis was carried out to reveal the distinct gene signatures upon 650 nm treatment. Results: Low-level 650 nm red light promoted the proliferation of human hair follicles in the experimental cultured-tissue model. Consistently, 650 nm red light significantly delayed the transition of hair cycle from anagen to catagen in vitro. RNA-seq analysis and gene clustering for the differentially expressed genes suggests that leukocyte transendothelial migration, metabolism, adherens junction and other biological process maybe involved in stimulation of hair follicles by 650-nm red light treatment. Conclusion: The effect of 650-nm red light on ex vivo hair follicles and the transcriptome set which implicates the role of red light in promoting hair growth and reversing of miniaturization process of AGA were identified.

Three new ent-abietane diterpenoids from the roots of Euphorbia fischeriana and their cytotoxicity in human tumor cell lines

Minghui Li,Fang He,Yuan Zhou,Meigui Wang,Pingde Tao,Qingmei Tu,Guanghui Lv,Xintao Chen 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.6

Three new ent-abietane diterpenoids, termed fischerianoids A–C (1–3), were isolated and identified from the ethyl acetate extracts of roots of the medicinally valuable plant Euphorbia fischeriana. The planar and relative structures of 1–3 were established via high-resolution electrospray ionisation mass spectrometry and one- and two-dimensional nuclear magnetic resonance spectroscopic analyses, and the absolute configuration of 1 was further established via X-ray crystallography experiment. Compounds 1–3 showed selective inhibitory potency against certain human tumor cell lines with IC50 values ranging from 8.50 ± 0.13 to 35.52 ± 0.08 μM.

The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs

Feng Chen,Yulin Ma,Chunyi Xue,Jingyun Ma,Qingmei Xie,Genhu Wang,Yingzuo Bi,Yongchang Cao 대한수의학회 2008 JOURNAL OF VETERINARY SCIENCE Vol.9 No.1

This study was to investigate the effects of the combination of deoxynivalenol (DON) and zearalenone (ZON) on pigs. Twenty-four weaning piglets were divided into a control group fed a diet free of mycotoxins and a toxin group fed a diet containing 1 mg/kg DON and 250 μg/kg ZON. The results showed that supplementation of DON and ZON in diets had extensive effects on pigs. More specifically, DON and ZON caused levels of total protein, albumin, and globulin in sera to decrease (p < 0.05) by 14.5%, 6.5% and 11.3%, respectively, and at the same time increased (p < 0.05) the serum enzyme activities of γ-glutamyltransferase, aspartate aminotransferase and alanine aminotransferase by 72.0%, 32.6% and 36.6%, respectively. In addition, DON and ZON decreased (p < 0.05) the level of anticlassical swine fever antibody titers by 14.8%. Real-time PCR showed that DON and ZON caused the mRNA expression levels of IFN-γ, TNF-α, IL-2, to decrease (p < 0.05) by 36.0%, 29.0% and 35.4%, respectively. Histopathological studies demonstrated that DON and ZON caused abnormalities in the liver, spleen, lymph nodes, uterus, and kidney. The concentrations of DON and ZON used in this study are in line with the published critical values permitted by BML. Our study clearly put the standard and adequacy of safety measures for these toxins into question. The authors suggest that with the increasing availability of cellular and molecular technologies, it is time to revisit the safety standards for toxins in feeds so as to make feeds safer, providing consumers with safer products. This study was to investigate the effects of the combination of deoxynivalenol (DON) and zearalenone (ZON) on pigs. Twenty-four weaning piglets were divided into a control group fed a diet free of mycotoxins and a toxin group fed a diet containing 1 mg/kg DON and 250 μg/kg ZON. The results showed that supplementation of DON and ZON in diets had extensive effects on pigs. More specifically, DON and ZON caused levels of total protein, albumin, and globulin in sera to decrease (p < 0.05) by 14.5%, 6.5% and 11.3%, respectively, and at the same time increased (p < 0.05) the serum enzyme activities of γ-glutamyltransferase, aspartate aminotransferase and alanine aminotransferase by 72.0%, 32.6% and 36.6%, respectively. In addition, DON and ZON decreased (p < 0.05) the level of anticlassical swine fever antibody titers by 14.8%. Real-time PCR showed that DON and ZON caused the mRNA expression levels of IFN-γ, TNF-α, IL-2, to decrease (p < 0.05) by 36.0%, 29.0% and 35.4%, respectively. Histopathological studies demonstrated that DON and ZON caused abnormalities in the liver, spleen, lymph nodes, uterus, and kidney. The concentrations of DON and ZON used in this study are in line with the published critical values permitted by BML. Our study clearly put the standard and adequacy of safety measures for these toxins into question. The authors suggest that with the increasing availability of cellular and molecular technologies, it is time to revisit the safety standards for toxins in feeds so as to make feeds safer, providing consumers with safer products.

Transcriptome-wide identification of WRKY transcription factor and their expression profiles under salt stress in sweetpotato (Ipomoea batatas L.)

Qin Zhen,Hou Fuyun,Li Aixian,Dong Shuxu,Wang Qingmei,Zhang Liming 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5

WRKY transcription factors play a key role in regulating plant growth, development, and stress responses. Although many WRKY transcription factors have been discovered in plants, the WRKY transcription factors in sweetpotato have not been systematically studied. Here, we identifed 79 IbWRKY transcription factors based on the transcriptome data of sweetpotato. The amino acid length of the protein ranged from 162 to 711 amino acids. Frequency analysis of amino acids in IbWRKY transcription factors found highest levels of serine. Subcellular localization analysis found 77 IbWRKY proteins localized in the nucleus. The IbWRKY proteins were clustered into three groups based on their structural and phylogenetic characteristics. Seven WRKY proteins contained the WRKY domain variations WRKYG(K/T)K as revealed by multiple sequence alignment. Structural analysis showed that IbWRKY proteins of the same subfamily had similar motifs. Transcriptome data analysis found that a total of 35 IbWRKY genes showed signifcant changes of the expression level after the 150 mM NaCl treatment. We selected 16 IbWRKY genes for real-time quantitative PCR verifcation and found that the data were consistent with the transcriptome data. In summary, this study provides a systematical analysis of the IbWRKY transcription factors, which will lay a solid foundation for further characterization of the regulatory mechanism of the IbWRKY genes under salt stress in sweetpotato.