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Qin Zhen,Hou Fuyun,Li Aixian,Dong Shuxu,Wang Qingmei,Zhang Liming 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5
WRKY transcription factors play a key role in regulating plant growth, development, and stress responses. Although many WRKY transcription factors have been discovered in plants, the WRKY transcription factors in sweetpotato have not been systematically studied. Here, we identifed 79 IbWRKY transcription factors based on the transcriptome data of sweetpotato. The amino acid length of the protein ranged from 162 to 711 amino acids. Frequency analysis of amino acids in IbWRKY transcription factors found highest levels of serine. Subcellular localization analysis found 77 IbWRKY proteins localized in the nucleus. The IbWRKY proteins were clustered into three groups based on their structural and phylogenetic characteristics. Seven WRKY proteins contained the WRKY domain variations WRKYG(K/T)K as revealed by multiple sequence alignment. Structural analysis showed that IbWRKY proteins of the same subfamily had similar motifs. Transcriptome data analysis found that a total of 35 IbWRKY genes showed signifcant changes of the expression level after the 150 mM NaCl treatment. We selected 16 IbWRKY genes for real-time quantitative PCR verifcation and found that the data were consistent with the transcriptome data. In summary, this study provides a systematical analysis of the IbWRKY transcription factors, which will lay a solid foundation for further characterization of the regulatory mechanism of the IbWRKY genes under salt stress in sweetpotato.