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Zhi-Fu Guo,Xiang-Yu Long,Pan Dong,Yu-Ming Wei,Li-Ping Bai,Xiao-Xuan Dang,Hao-Lei Wan,Li-Jun Zhang,You-Liang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2
The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C.delileana had obvious difference with those from common wheat in α, γand ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1,gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae,phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.
Hai-Peng Xu,Wan-Zhong Lang,Xuan Zhang,Ya-Jun Guo 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
The charged poly(vinylidene fluoride) (PVDF) membranes were prepared by composite thermallyinduced phase separation (C-TIPS) method with perfluorosulfonic acid (PFSA) as additive. The resultsverify that the porosity, surface hydrophilicity and pure water permeation flux (JW) increase with theaddition of PFSA for the membranes. The highest JW of 507.2 1.2 L/(M2 H bar) is obtained for themembrane cast at 90 8C. Much higher casting temperature isn’t advantageous to improve JW value for thePVDF membranes. All membranes exhibit negative apparent zeta potential (zapp). The crystallinity in b phaseincreases as casting temperature increases for all PVDF membranes.
Ying-Wang,Yi-guo Chen,Wan-shuang Zhao,Di Li,Ya-han Xu,Meng-di Li,Jin Chen,Zhi-jun Kou,Qi-ge Wang,Nsoa dimitri Joseph 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.3
This study aims to investigate the possible mechanisms of electroacupuncture (EA) at PC6to improve myocardial ischemia (MI) by regulating the cardiac transient outward potassiumcurrent channel (Ito). According to the random number table, the mice were dividedinto six groups of six mice each: control group, MI group, PC6, LU7 (Lieque-point), ST36(Zusanli-point), and nonacupoint group. Mice in the control group were injected with saline(20 mg/kg, 24 hours interval), and the other ASIC3 / mice were injected subcutaneouslytwice with isoproterenol (ISO) (20 mg/kg, 24 hours interval). In thepreexperiment, 5 mg/kg, 10 mg/kg, 20 mg/kg, and 30 mg/kg of ISO were used, andthe results showed that 5 mg/kg and 10 mg/kg of ISO both could induce acute MI, butshorter duration of sustained MI. On the other hand, an injection of 30 mg/kg can makethe mice experience arrhythmia or die immediately, and EA was operated at PC6, LU7,ST36 acupoints, and nonacupoint in the mice of PC6, LU7, ST36, and nonacupoint groups,respectively, after injecting twice. Then Western blotting techniques (Western Blot) wereused to analyze the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KchIP2. The results ofthis experiment showed that the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KChIP2 inMI group were significantly lower than those in the control group (p < 0.01). Comparedwith MI group, the results of PC6, LU7, and ST36 groups obviously increased (p < 0.05). Furthermore, the expressions of PC6 group were higher than LU7 group and ST36 group(p < 0.05). And electrocardiogram’s T-waves showed obvious pathological changes inthe MI group compared to the control group (p < 0.01). After EA, the abnormal T-waves voltage of ECG in PC6, LU7, and ST36 groups was improved (p < 0.05). In addition, therate change of PC6 group was larger than that of both LU7 and ST36 groups (p < 0.05). But the T-waves voltage of the nonacupoint group was not significantly different than thatof the MI group (p > 0.05).
Chen Xu,Li Guo-Qing,Wan Pin-Jun,Fu Qiang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
In the brown planthopper Nilaparvata lugens, introduction of double-stranded RNA (dsRNA) successfully knocks down genes transcribed in several types of tissues. However, whether RNA interference (RNAi) can effectively reduce the transcripts of neuronally-expressed genes remains undetermined. In the present paper, we selected three neuronally-expressed genes respectively encoding prothoracicotropic hormone (NlPTTH), pigment-dispersing factor (NlPDF) and short neuropeptide F (NlsNPF). The transcripts of the three genes were detectable throughout all tested developmental stages, and were mainly restricted to the nervous system of N. lugens. We introduced dsRNA originated from each of the three neuronally-expressed genes at a dose of 300 ng into the nymphal body cavity by microinjection. The target mRNA was successfully knocked down. Moreover, silencing of NlPTTH downregulated the transcripts of two ecdysteroidogenesis genes (NlPHM and NlDIB), and surpressed the expression of an ecdysone-response genes (NlFTZ-F1). Furthermore, RNAi of NlPTTH caused typical 20- hydroxyecdysone deficient phenotypes: nymphal development was delayed and nymphal lethality occurred. In addition, injection of a series of dsNlsDPF solutions at the doses of 300, 60, 12, 2.4 and 0.5 ng into the fourthinstar nymphs decreased its target transcript in a dose-dependent manner: the mRNA levels reduced by 94%, 86%, 57%, 33% and 29%, respectively. Therefore, delivery of dsRNA by microinjection can effectively repress the transcripts of the neuronally-expressed genes in N. lugens.
Liu-Liu Long,Ke Xia,Wan-Zhong Lang,Li-Ling Shen,Qiang Yang,Xi Yan,Ya-Jun Guo 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.51 No.-
The zirconia, alumina, and zirconia-alumina (xZr-Al) supported PtSnIn catalysts were prepared andcomparatively studied for propane dehydrogenation reaction. The structure–function relationships ofthe resultant catalysts are well elucidated upon several state-of-art physico-chemical characterizations. The results show that xZr-Al used as support for trimetallic PtSnIn catalysts exhibits the significantcatalytic performances. The PtSnIn/08Zr-Al catalyst has the highest Pt dispersion accompanied with thesmallest average Pt particles, and it presents the initial propane conversion and propylene selectivityabove 55.0% and 98.0% respectively. The initial propane conversions afterfive reaction–regenerationcycles are all above 55.0% and only decrease little ( 3%).
Han Wang,Zuo-Ming Wang,Xi Yan,Jun Chen,Wan-Zhong Lang,Ya-Jun Guo 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.52 No.-
Novel polyvinylidenefluoride (PVDF) hybrid ultrafiltration membranes with antifouling and antibacterialproperties are prepared by embedding N-halamine functionalized silica nanospheres (HFSNs). With theaddition of HFSNs, the antifouling properties of PVDF membranes are significantly improved. The resultsreveal that the highest pure water permeationflux of 559.8 L m 2 bar 1 h 1 is attained when the 0.6 wt%HFSNs is added in the casting solution. The membrane of M-3 with 0.9 wt% HFSNs addition shows thehighest sterilization ratios of 97.1% and 97.0% against (escherichia coli) E. coli and (staphylococcus aureus)S. aureus respectively. After 6 times of inhibition-activation cycles, the membrane still remains 72.3%against E.coli.
The Gender-Sensitive Social Risk Factors for Internet Addiction in College Undergraduate Students
Xia Lin,Jing-yan Gu,Wan-jun Guo,Ya-jing Meng,Hui-yao Wang,Xiao-jing Li,Wei Deng,Lian-sheng Zhao,Xiao-hong Ma,Ming-li Li,Ting Chen,S,K,Cheng,Tao Li 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.7
Objective The current study aims to explore precipitating and social risk factors for internet addiction (IA) in university undergraduate students, and to provide evidence for interventions and the early prevention of IA in different genders. Methods Four thousand eight hundred and fifty-eight college sophomores completed an online survey on their internet use-related behaviours and social risk factors. Results We found that more male (8.3%) than female students (5.4%) had moderate and severe IA. The main online activity in the moderate and severe IA groups was online gaming in males and online streaming in females. Roommates engaging in similar internetbased entertainment was a risk factor of IA only for males, while not being in a romantic relationship was a risk factor of IA for females only. Infatuation with the internet before college and adjustment problems for college life were shared risk factors for both genders in the mild and moderate IA groups. Conclusion IA was a common phenomenon in college students with shared and unique precipitating and social risk factors in males and females. The gender-sensitive risk factors for IA warranted earlier and individualized intervention and prevention strategies for IA in this population.