Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana

Xia Fang,Juan Shen,Jie Wang,Zhi-li Chen,Pei-bin lin,Zhi-yu Chen,Lin-yan Liu,Huan-xiong Zeng,Xiao-bao Jin 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

Actinomycetes are well-known for producing numerous bioactive secondary metabolites. In this study, primary screening by antifungal activity assay found one actinomycete strain WA23-4-4 isolated from the intestinal tract of Periplaneta americana that exhibited broad spectrum antifungal activity. 16S rDNA gene analysis of strain WA23-4-4 revealed close similarity to Streptomyces nogalater (AB045886) with 86.6% sequence similarity. Strain WA23-4-4 was considered as a novel Streptomyces and the 16s rDNA sequence has been submitted to GenBank (accession no. KX291006). The maximum antifungal activity of WA23-4-4 was achieved when culture conditions were optimized to pH 8.0, with 12% inoculum concentration and 210 ml ISP2 medium, which remained stable between the 5th and the 9th day. 3-Acetyl benzoyl amide was isolated by ethyl acetate extraction of WA23- 4-4 fermentation broth, and its molecular formula was determined as C9H9NO2 based on MS, IR, 1H, and 13C NMR analyses. The compound showed significant antifungal activity against Candida albicans ATCC 10231 (MIC: 31.25 μg/ml) and Aspergillus niger ATCC 16404 (MIC: 31.25 μg/ml). However, the compound had higher MIC values against Trichophyton rubrum ATCC 60836 (MIC: 500 μg/ml) and Aspergillus fumigatus ATCC 96918 (MIC: 1,000 μg/ml). SEM analysis showed damage to the cell membrane of Candida albicans ATCC 10231 and to the mycelium of Aspergillus niger ATCC 16404 after being treatment with 3-acetyl benzoyl amide. In conclusion, this is the first time that 3-acetyl benzoyl amide has been identified from an actinomycete and this compound exhibited antifungal activity against Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404.

ENTERPRISE INCOME TAX LAW

XIA LIN 한국국제조세협회 2008 조세학술논집 Vol.24 No.2

Research on the Protection Scheme of DG Interconnection Transition Mode Based on Decoupling Zero Sequence

Xia Lin,Yuping Lu,Yao Li,Guibin Zou 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.8

More and more DG is connected with the grid through the low voltage distribution network, how to choose a protection scheme without depending on the nature of DG, and with reliable operation mode is a big issue encountered by the engineers. The rationally allocating the protection scheme of grid connection coupling point, which not only can judge the system fault in time, and judge it in DG island, and can still remain the protection features in the dynamic process of the island formation and in the operation mode of dividing island, can always have enough selectivity of the island fault is the problem to solve in this study. Through the selection of the grounding methods of DG grid connection transformer, the relationship between zero sequence current and zero sequence voltage is analyzed; the optimization of the protection scheme can inhibit DG interconnection and zero sequence over-voltage in network running-off, and quantity of zero sequence can be used as a stable criterion when the fault occurs, so as to work out a set of protection optimization schemes for DG interconnection transformer grounding system. Finally, through the COBASE system, a protection scheme of the wind power plant low-voltage grid is generated in this study, which verifies its excellent protection features in the DG connected to the grid system.

The Gender-Sensitive Social Risk Factors for Internet Addiction in College Undergraduate Students

Xia Lin,Jing-yan Gu,Wan-jun Guo,Ya-jing Meng,Hui-yao Wang,Xiao-jing Li,Wei Deng,Lian-sheng Zhao,Xiao-hong Ma,Ming-li Li,Ting Chen,S,K,Cheng,Tao Li 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.7

Objective The current study aims to explore precipitating and social risk factors for internet addiction (IA) in university undergraduate students, and to provide evidence for interventions and the early prevention of IA in different genders. Methods Four thousand eight hundred and fifty-eight college sophomores completed an online survey on their internet use-related behaviours and social risk factors. Results We found that more male (8.3%) than female students (5.4%) had moderate and severe IA. The main online activity in the moderate and severe IA groups was online gaming in males and online streaming in females. Roommates engaging in similar internetbased entertainment was a risk factor of IA only for males, while not being in a romantic relationship was a risk factor of IA for females only. Infatuation with the internet before college and adjustment problems for college life were shared risk factors for both genders in the mild and moderate IA groups. Conclusion IA was a common phenomenon in college students with shared and unique precipitating and social risk factors in males and females. The gender-sensitive risk factors for IA warranted earlier and individualized intervention and prevention strategies for IA in this population.

Anisotropic In-plane Resistivity and Magnetoresistance of the Detwinned BaFe2As2

Lin Jiao,Zong Fa Weng,Xue Yan Tang,Lu Kai Guo,Tian Shang,Lin Yang,Hui Qiu Yuan,Yu Ying Wu,Zheng Cai Xia 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3

We have measured the electrical resistivity and the magnetoresistance of detwinned BaFe2As2single crystals in fields up to 16 Tesla. The temperature dependence of the resistivity shows acounterintuitive anisotropic behavior in the ab-plane, likely arising from the effect of the nematicsusceptibility. Little magnetoresistance is observed at temperatures above the structural/magnetictransition, below which a huge in-plane magnetoresistance with an anisotropy is detected.

Geographic Distribution and Epidemiology of Lung Cancer During 2011 in Zhejiang Province of China

Lin, Xia-Lu,Chen, Yan,Gong, Wei-Wei,Wu, Zhao-Fan,Zou, Bao-Bo,Zhao, Jin-Shun,Gu, Hua,Jiang, Jian-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: To explore etiology for providing scientific clues for the prevention of lung cancer. Materials and Methods: Data for lung cancer incidence and meteorological geographic factors from 25 counties in Zhejiang province of China during 2011 were studied. Stepwise multiple regression and correlation analysis were performed to analyze the geographic distribution and epidemiology of lung cancer. Results: 8,291 new cases (5,998 in males and 2,293 females) of lung cancer during 2011 in Zhejiang province were reported in the 25 studied counties. Reported and standardized incidence rates for lung cancer were 58.0 and 47.0 per 100,000 population, respectively. The incidence of lung cancer increased with age. Geographic distribution analysis shows that the standardized incidence rates of lung cancer in northeastern Zhejiang province were higher than in the southwestern part, such as in Nanhu, Fuyang, Wuxing and Yuyao counties, where the rates were more than 50 per 100,000 population. In the southwestern Zhejiang province, for instance, in Yueqing, Xianju and Jiande counties, the standardized incidence rates of lung cancer were lower than 37 per 100,000 population. Spearman correlation tests showed that forest coverage rate, air quality index (AQI), and annual precipitation level are associated with the incidence of lung cancer. Conclusions: Lung cancer in Zhejiang province shows obvious regional differences. High incidence appears associated with low forest coverage rate, poor air quality and low annual precipitation. Therefore, increasing the forest coverage rate and controlling air pollution may play an important role in lung cancer prevention.

Dynamic analysis of coupled train - ladder track - elevated bridge system

Xia, He,Deng, Yushu,Xia, Chaoyi,De Roeck, G.,Qi, Lin,Sun, Lu Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.5

As a new type of vibration reduction, the ladder track system has been successfully used in engineering. In this paper, a numerical model of the train-track-viaduct system is established to study the dynamic responses of an elevated bridge with ladder track. The system is composed of a vehicle submodel, a track submodel and a bridge submodel, with the measured track irregularities as the system self-excitation. The whole time histories of a train running through an elevated bridge with $3{\times}27m$ continuous PC box girders are simulated. The dynamic responses of the bridge such as deflections, lateral and vertical accelerations, and the vehicle responses such as derailment factors, offload factors and car-body accelerations are calculated. The calculated results are partly validated through the comparison with the experimental data. Compared to the common slab track, adapting the ladder sleeper can effectively reduce the accelerations of the bridge girder, and also reduce the car-body accelerations and offload factors of the train vehicle.

Preparation and Characterization of Poly(lactic acid) Micro- and Nanofibers Fabricated by Centrifugal Spinning

Lei Xia,Lin-lin Lu,Yu-Xia Liang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

In this paper, poly(lactic acid) (PLA) micro- and nanofibers were successfully prepared by centrifugal spinningwhich is a more simple and effective approach to produce micro/nanofibers. The morphology of fibers was investigated usingscanning electron microscopy (SEM), X-ray diffraction (XRD), while their thermal properties by differential scanningcalorimeter (DSC) and thermal gravimetric analysis (TGA). The results showed that the diameter of PLA fibers increasedsignificantly with increasing solution concentration and decreased slightly with increasing collection distance. It can becontrolled below 500 nm at the optimal process parameters which were found to be spinning solution concentration of 6 %,spinneret diameter of 0.11 mm, rotational speed of 5500 rpm and collecting distance of 15 cm. Although the crystallinity ofthe PLA fibers was weaker than that of the PLA pellets due to the insufficient adjustment of the molecular chain in rapidsolvent evaporation and short stretching time, it can be improved by increasing the rotational speed from 4500 to 7000 rpm. The glass transition temperature (Tg) and melting temperature (Tm) of the fibers were nearly keeping constant compared tothe PLA pellets. Its good degradability was demonstrated by the loss of fibers structure completely within 5 hours ofimmersion in NaOH at pH 13.

中国房产税功能定位及制度要素设计

Wang Lin Xia(王金霞),Kim Chun Soo(金春洙) 대한경영교육학회 2016 경영교육저널 Vol.27 No.1

Under the current circumstance such as the lack of main source of local taxes, the instability of estate market and unfair distribution of social resources. The public have expect too much of the real estate tax and give a lot of functions to it, such as supplementing the local tax revenue, regulating the real estate market, adjusting income distribution and so on, which makes the estate tax reform have developed slowly. Meanwhile, the real estate tax reform involves the violation of private property, so the real estate tax reform will inevitably face " multilateral game ". The paper explores the fundamental situation of real estate tax as a part of the local fiscal revenue abroad by analyzing the contribution made by real estate tax to local fiscal revenue. On this basis, the paper concludes that the existing and growing real estate is an abundant source of real estate tax by observing the development of the real estate market, which is the economic basis that makes the real estate tax become an important part of the local tax system. Since the real estate tax reform involves the violation of private property, not only do we consider the financial demand of tax authority from a macro perspective but also discuss the economic duration and political acceptability, which makes real estate reform become a gradual process. 针对中国目前地方主体税种缺失、房地产市场不稳、社会分配不公等现实情况, 社会各个层面赋予房产税弥补地方税缺失、调节房地产市场、调节居民收入分配等诸多功能, 人们对房产税改革赋予过多的功能和期望, 使得房产税改革在各种期望的"纠结"中踯躅前行。同时, 房产税改革涉及对私有财产的"侵犯", 房产税改革必然面临着"博弈"。本文从分析国外房地产税对地方政府税收收入贡献入手, 探讨国外房产税作为地方财政收入的基本状况。在此基础上, 观测中国房地产市场多年的发展, 认为业已存在的存量房产及新增房产为房产税储备的丰腴的税源, 是房产税承担地方税重要税种的现实经济基础。由于房产税改革涉及对私有财产的"侵犯", 因此, 在制度要素设计方面, 不仅要从宏观角度考量征税主体财政功能的诉求, 还要从微观角度考察纳税主体的经济承受力和政治接受度, 可见房税改革将是一个渐进而漫长的过程。

Ischemic postconditioning protects cardiomyocytes against ischemia/reperfusion injury by inducing MIP2

Hong-Lin Zhu,Kang-Kai Wang,Xing Wei,Shun-Lin Qu,Chi Zhang,Xiao-Xia Zuo,Yan-Sheng Feng,Qi Luo,Guang-Wen Chen,Mei-Dong Liu,Lei Jiang,Xian-Zhong Xiao 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.8

Cardiomyocytes can resist ischemia/reperfusion (I/R)injury through ischemic postconditioning (IPoC)which is repetitive ischemia induced during the onset of reperfusion. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a member of the WD-40family proteins, we previously showed that MIP2 was up-regulated during ischemic preconditioning (IPC). As IPC and IPoC engaged similar molecular mechanisms in cardioprotection, this study aimed to elucidate whether MIP2 was up-regulated during IPoC and contributed to IPoC-mediated protection against I/R injury. The experiment was conducted on two models,an in vivo open chest rat coronary artery occlusion model and an in vitro model with H9c2 myogenic cells. In both models, 3 groups were constituted and randomly designated as the sham, I/R and IPoC/hypoxia postconditioning (HPoC) groups. In the IPoC group, after 45 min of ischemia, hearts were allowed three cycles of reperfusion/ischemia phases (each of 30 s duration)followed by reperfusion. In the HPoC group, after 6 h of hypoxia, H9c2 cells were subjected to three cycles of 10 minute reoxygenation and 10 minute hypoxia followed by reoxygenation. IPoC significantly reduced the infarct size, plasma level of Lactate dehydrogenase and creatine kinase MB in rats. 12 h after the reperfusion,MIP2 mRNA levels in the IPoC group were 10 folds that of the sham group and 1.4 folds that of the I/R group. Increased expression of MIP2 mRNA and attenuation of apoptosis were similarly observed in the HPoC group in the in vitro model. These effects were blunted by transfection with MIP2 siRNA in the H9c2cells. This study demonstrated that IPoC induced protection was associated with increased expression of MIP2. Both MIP2 overexpression and MIP2 suppression can influence the IPoC induced protection.