OK-432 Suppresses Proliferation and Metastasis by Tumor Associated Macrophages in Bladder Cancer

Tian, Yuan-Feng,Tang, Kun,Guan, Wei,Yang, Tao,Xu, Hua,Zhuang, Qian-Yuan,Ye, Zhang-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

OK-432, a Streptococcus-derived anticancer immunotherapeutic agent, has been applied in clinic for many years and achieved great progress in various cancers. In the present study, we investigated its anticancer effect on bladder cancer through tumor associated macrophages (TAMs). MTS assay validated OK-432 could inhibit proliferation in both T24 and EJ bladder cell lines. OK-432 also induced apoptosis of bladder cancer cells in vitro. Consequently, we demonstrated that OK-432 could suppress the bladder cancer cells migration and invasion by altering the EMT-related factors. Furthermore, using SD rat model, we revealed that OK-432 inhibited tumor growth, suppressed PCNA expression and inhibited metastasis in vivo. Taken together, these findings strongly suggest that OK-432 inhibits cell proliferation and metastasis through inducing macrophages to secret cytokines in bladder cancer.

CHISELED NICKEL HYDROXIDE NANOPLATES GROWTH ON GRAPHENE SHEETS FOR LITHIUM ION BATTERIES

LEI-LEI TIAN,XIAN-YONG WEI,QUAN-CHAO ZHUANG,CHAO WU,RUI-LUN XIE,ZHI-MIN ZONG,YONG-LI CUI,SHI-GANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.

Optical properties and radiative forcing of urban aerosols in Nanjing, China

B.L. Zhuang,T.J. Wang,S. Li,R. Talbot,H.T. Mao,X.Q. Yang,C.B. Fu,C.Q. Yin,J.L. Zhu,J. Tian,X.Y. Zhang,H.Z. Che 한국대기환경학회 2013 한국대기환경학회 학술대회논문집 Vol.2013 No.8

Population-Stratified Analysis of Bone Mineral Density Distribution in Cervical and Lumbar Vertebrae of Chinese from Quantitative Computed Tomography

Yong Zhang,Zhuang Zhou,Cheng’ai Wu,Danhui Zhao,Chao Wang,Xiaoguang Cheng,Wei Cai,Ling Wang,Yangyang Duanmu,Chenxin Zhang,Wei Tian 대한영상의학회 2016 Korean Journal of Radiology Vol.17 No.5

Objective: To investigate the bone mineral density (BMD) of cervical vertebrae in a population-stratified manner and correlate with that of the lumbar vertebrae. Materials and Methods: Five hundred and ninety-eight healthy volunteers (254 males, 344 females), ranging from 20 to 64 years of age, were recruited for volumetric BMD (vBMD) measurements by quantitative computed tomography. Basic information (age, height, weight, waistline, and hipline), and vBMD of the cervical and lumbar vertebrae (C2–7 and L2–4) were recorded. Comparisons among sex, age groups and different levels of vertebrae were analyzed using analysis of variance. Linear regression was performed for relevance of different vertebral levels. Results: The vBMD of cervical and lumbar vertebrae was higher in females than males in each age group. The vBMD of the cervical and lumbar vertebrae in males and the vBMD of lumbar vertebrae in females decreased with aging. In each age group, the vBMD of the cervical vertebrae was higher than that of the lumbar vertebrae with gradual decreases from C2 to C7 except for C3; moreover, the vBMD of C6 and C7 was significantly different from that of C2–5. Correlations of vBMD among different cervical vertebrae (females: r = 0.62–0.94; males: r = 0.63–0.94) and lumbar vertebrae (males: r = 0.93–0.98; females: r = 0.82–0.97) were statistically significant at each age group. Conclusion: The present study provided normative data of cervical vertebrae in an age- and sex-stratified manner. Sex differences in vBMD prominently vary with age, which can be helpful to design a more comprehensive pre-operative surgical plan.

근육 피로에서 Flavonoids의 잠재적 역할: a review

왕영(WANG YING),전장(TIAN ZHUANG),이진우(LI ZHENYU),김재철(Kim, Jae-Cheol) 한국체육과학회 2023 한국체육과학회지 Vol.32 No.1

Study on heat transfer characteristics and structural parameter effects of heat pipe with fins based on MOOSE platform

Chen Xiaoquan,Du Peng,Tian Rui,Li Zhuoyao,Lian Hongkun,Zhuang Kun,Wang Sipeng 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.1

The space reactor is the primary energy supply for future space vehicles and space stations. The radiator is one of the essential parts of a space reactor. Therefore, the research on radiators can improve the heat dissipation power, reduce the quality of radiators, and make the space reactor smaller. Based on MOOSE multi-physics numerical calculation platform, a simulation program for the combination of heat pipe and fin at the end of heat pipe radiator is developed. It is verified that the calculation result of this program is accurate and the calculation speed is fast. Analyze the heat transfer characteristics of the combination with heat pipe and fin, and obtain its internal temperature field. Based on the calculation results, the influence of structural parameters on the heat dissipation power is analyzed. The results show that when the fin width is 0.25 m, fin thickness is 0.002 m, condensing section length is 0.5425 m and heat pipe radius is 0.014 m, the power-mass ratio is the highest. When the temperature is 700Ke900K, the heat dissipation power increases 41.12% for every 100K increase in the operating temperature. Smaller fin width and thinner fin thickness can improve the power-mass ratio and reduce the radiator quality.

Exercise-mediated macrophage polarization modulates the targeted therapeutic effect of NAFLD: a review

Li Zhenyu,Wang Ying,Tian Zhuang,Xie Yongchao,김재철 한국운동영양학회 2023 Physical Activity and Nutrition (Phys Act Nutr) Vol.27 No.3

[Purpose] This review aims to explore the exercise-mediated hepatic macrophage polarization mechanism and its effect on improving and regulating non-alcoholic fatty liver disease (NAFLD) by analyzing the pathogenesis of NAFLD and the cause of the influence of hepatic macrophage polarization. In addition to exploring the varied effects of different exercise types on macrophage polarization regulation in NAFLD, to provide a direction and basis for the treatment of NA- FLD. [Methods] The research methodology involved a comprehensive search of the PubMed database using specific keywords such as “NAFLD”, “macrophage polarization”, and “exercise”, to retrieve relevant literature published. [Results] (1) The main factors inducing NAFLD were high-fat diet, obesity, insulin resistance (IR), changes in gut microbiota, and genetic variation in susceptibility. (2) Drug treatment, nutrient induction, microfactor induction, physiological environment induction, and other factors can induce the polarization of hepatic macrophages and affect NAFLD. (3) Different intensities, types, and frequencies of exercise have different effects on polarization macrophages, and may also differently effects improving liver inflammation, fibrosis, and NAFLD. Curently, regular moderate-intensity aerobic exercise is the most effective therapy for treating NAFLD. [Conclusion] Approaches to ameliorate NAFLD with exercise involve strategies to alter macrophage polarization by inhibiting M1 or driving M2 activation. However, research on the different types of exercise-mediated macrophage polarization mechanisms and differences in therapeutic effects is not yet sufficient. Future research is necessary to explore the exact mechanisms and differences in the effects of different exercises on the treatment of NAFLD.

Effect of Increasing Diffusion Gradient Direction Number on Diffusion Tensor Imaging Fiber Tracking in the Human Brain

Xufeng Yao,Tonggang Yu,Beibei Liang,Tian Xia,Qinming Huang,Songlin Zhuang 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.2

To assess the effects of varying the number of diffusion gradient directions (NDGDs) on diffusion tensor fiber tracking (FT) in human brain white matter using tract characteristics. Twelve normal volunteers underwent diffusion tensor imaging (DTI) scanning with NDGDs of 6, 11, 15, 21, and 31 orientations. Three fiber tract groups, including the splenium of the corpus callosum (CC), the entire CC, and the full brain tract, were reconstructed by deterministic DTI-FT. Tract architecture was first qualitatively evaluated by visual observation. Six quantitative tract characteristics, including the number of fibers (NF), average length (AL), fractional anisotropy (FA), relative anisotropy (RA), mean diffusivity (MD), and volume ratio (VR) were measured for the splenium of the CC at the tract branch level, for the entire CC at tract level, and for the full brain tract at the whole brain level. Visual results and those of NF, AL, FA, RA, MD, and VR were compared among the five different NDGDs. The DTI-FT with NDGD of 11, 15, 21, and 31 orientations gave better tracking results compared with NDGD of 6 after the visual evaluation. NF, FA, RA, MD, and VR values with NDGD of six were significantly greater (smallest p = 0.001 to largest p = 0.042) than those with four other NDGDs (11, 15, 21, or 31 orientations), whereas AL measured with NDGD of six was significantly smaller (smallest p = 0.001 to largest p = 0.041) than with four other NDGDs (11, 15, 21, or 31 orientations). No significant differences were observed in the results among the four NDGD groups of 11, 15, 21, and 31 directions (smallest p = 0.059 to largest p = 1.000). The main fiber tracts were detected with NDGD of six orientations; however, the use of larger NDGD (≥ 11 orientations) could provide improved tract characteristics at the expense of longer scanning time.

Nitro-oleic Acid Decreases Transcription of the Angiotensin II Type I Receptor Gene in Aortic Smooth Muscle Cells

Huan Wang,Hongsheng Ouyang,Yaping Tian,Zhuang Liu,Xiaolei Han,Xingxing Liu,Guangyao Ran,Gangqi Wang,Daxin Pang,Xiaochun Tang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4

Nitroalkene derivatives of nitro-oleic acid(OA-NO2) regulate pluripotent cell signaling in vivo underphysiological and pathological conditions. Angiotensin IItype 1 receptor (AT1R) plays an important role in thecardiovascular system. In this study, OA-NO2 reduced theAT1R mRNA level, specifically in primary smooth musclecells, showing a 70% reduction in rat smooth muscle cells(RASMCs) and a 50% reduction in pig smooth musclecells (PASMCs). These effects were not observed in CHOcells, which highly express AT1R. The AT1R mRNA decayrate was unchanged after OA-NO2 compared with OAtreatment. Nitric oxide (NO) and peroxisome proliferatoractivatedreceptor gamma (PPARγ) did not alter thereduced effects of OA-NO2 on the AT1R mRNA level inSMCs. However, Sp1-mediated activation of the AT1Rpromoter was reduced in response to OA-NO2 in RASMCsbut not 293T cells. In addition, the nuclear factor-kappa B(NF-κB) pathway was involved in the OA-NO2-mediateddownregulation of AT1R transcription in SMCs. Takentogether, our results demonstrate that OA-NO2 specificallyinhibits AT1R mRNA expression in primary smooth musclecells via the NF-κB pathway.

Enhancing the performance of LSSVM model in predicting rock fragmentation size via optimization algorithms

Haowen Zheng,Jun Liu,Ruihong Zhuang,Fu-tian Zhao,Meng-yang Zhen,Yue Wang,Zheng Liu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.9

The prediction of rock fragmentation is critical to improve the efficiency and economy of blasting excavation. In this study, an attempt is made to predict the entire fragmentation size distribution using a least square support vector machine (LSSVM) model. In addition, three optimization algorithms – the bacterial foraging algorithm (BFO), artificial fish swarm algorithm (AFSA), and adaptive particle swarm optimization (APSO) – were used to determine the appropriate parameters of the LSSVM model. In the constructed LSSVM-BFO, LSSVM-AFSA and LSSVM-APSO models, the hole spacing, row spacing, change per delay and stemming were used as the input parameters, while the statistical rock fragmentation size was assigned as the output. The LSSVM model was also employed as a control group for comparing with the optimized models. The above-mentioned models were trained and tested based on a database comprising of 10 datasets collected from in-site testing of Altashi Water Control Project in China. The performance of the proposed models was compared by several statistical criteria. The viability and efficiency of the LSSVM-BFO model were confirmed with an R2 of 0.9960 and an RMSE of 1.8044, which were better than those of the LSSVM-AFSA, LSSVM-APSO and LSSVM. Last but not least, sensitivity analysis was also executed. The result of sensitivity analysis demonstrated when the size of rock fragmentation of prediction is less 80mm, the most effective parameter will be stemming; otherwise the most effective parameter will be hole spacing.