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Acid etching of glass-infiltrated zirconia and its biological response
Van Thi Vu,Gye-Jeong Oh2,Kwi-Dug Yun,Hyun-Pil Lim,Ji-Won Kim,Thao Phuong Thi Nguyen,Sang-Won Park 대한치과보철학회 2017 The Journal of Advanced Prosthodontics Vol.9 No.2
PURPOSE. The purpose of this study was to evaluate the influence of acid etching treatment on surface characteristics and biological response of glass-infiltrated zirconia. MATERIALS AND METHODS. A hundred zirconia specimens were divided into four groups depending on surface treatments: untreated zirconia (group Z); acid-etched zirconia (group ZE); glass-infiltrated zirconia (group ZG); and glass-infiltrated and acid-etched zirconia (group ZGE). Surface roughness, surface topography, surface morphology, and Vickers hardness of specimens were evaluated. For biological response test, MC3T3-E1 cell attachment and proliferation on surface of the specimens were examined. The data were statistically analyzed using one-way ANOVA and Tukey’s HSD test at a significance level of 0.05. RESULTS. Group ZGE showed the highest surface roughness (Ra = 1.54 μm) compared with other groups (P < .05). Meanwhile, the hardness of group Z was significantly higher than those of other groups (P < .05). Cell attachment and cell proliferation were significantly higher in group ZGE (P < .05). CONCLUSION. We concluded that effective surface roughness on zirconia could be made by acid etching treatment after glass infiltration. This surface showed significantly enhanced osteoblast cell response.
Molecular biological analysis of Bt-9 rice and its effect on Daphnia magna feeding
Sung-Dug Oh,Soo-Yun Park,Sang Jae Suh,Ancheol Chang 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Insect-resistant transgenic (Bt-9) rice was generated by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis into the genome of a conventional variety of rice (Ilmibyeo). With regard to the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt-transgenic rice (Bt-9) and its near non-GM counterparts (Ilmibyeo) grown in the same environment (100% ground rice suspension). The Bt-9 rice was comfirmed to have the insertion of T-DNA and protein expression by the PCR and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of Daphnia magna between Bt-9 rice and Ilmibyeo. 48hr-EC50 values of Bt-9 and Ilmi rice showed 4,400 mg/L (95% confidence limits: 3861.01 ~ 5015.01 mg/L) and 5,564mg/L (95% confidence limits: 4780.03 ~ 6476.93 mg/L) respectively. The rice NOEC (No observed effect concentration) value for D. magna was suggested 1,620mg/L. We conclude that the tested Bt-9 and Ilmi similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt-9 didn’t affect to non-target insects.
Analysis of Removal Torque of Injection Molded Zirconia Implants; An Experimental Study on Beagles.
Oh, Gye-Jeong,Ban, Jae-Sam,Lim, Hyun-Pil,Yun, Kwi-Dug,Lee, Kwang-Min,Vang, Mong-Sook,Yang, Hong-So,Kang, Seong-Soo,Shin, Jin-Ho,Kim, Ga-Hyun,Ji, Min-Kyung,Park, Sang-Won,Fisher, John G American Scientific Publishers 2015 Journal of nanoscience and nanotechnology Vol.15 No.1
<P>This study compared the removal torque between injection molded zirconia implants and titanium implants with resorbable blast media (RBM) surfaces in beagle humeri. Fifteen screw-shaped implants were classified into 3 groups; titanium implant with RBM surface (Group RT), injection molded zirconia implant (Group Zr) and injection molded zirconia implant with sand-blasted surface (Group ZrS). Implants were inserted into beagle humeri. After 12 weeks, removal torque values were measured. The Zr group has a slightly higher removal torque value than the RT and ZrS groups but there were no significant differences among groups. Zirconia implants shows a similar removal torque to RBM titanium implants. This in vivo study showed injection molded zirconia implants could be an alternative to RBM titanium implants in terms of removal torque.</P>