Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가

이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.

아연계 탈황제의 장기연속 반응성 연구

박노국,이종욱,이승철,이종대,류시옥,이태진 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2

The zinc titanate sorbents(ZTG40) were prepared and the tests for durability and attrition resistance were carried out to develop the highly effective desulfurization sorbents. In the attrition tests for ZTG40 the results showed that the attrition resistance was 94.08% and that the sulfur capacity was maintained above 15g S/100g sorbent throughout the 100-cycle test, its maximum being 23.05g S/100g sorbent. As compared to sorbents developed by the developed countries, ZTG40 showed superior performances. In the advanced countries 15g S/100g sorbent is a yardstick of excellency for the sulfur capacity of desulfurization sorbents. Therefore, ZTG40 developed in this study has the desirable physical and chemical properties for fluidized desulfurization of coal gases.

발전기 고정자 권선용 마이카/에폭시 수지의 표면 및 구조분석

이상교,심재선,정의남,송우창,박하용,심상흥,임윤희,박종국,심낙순 三陟大學校 1998 論文集 Vol.31 No.1

The purpose of this study is to determine the ageing mechanism of Mica/Epoxy composite material for large generator stator windings in order to estimate remaining life of generator. X-ray analysis is performed to investigate the position and structure of mica crystal in insulation materials. The phase change of aged and sound specimen under each atmosphere isn't observed. Surface analysis of insulation materials by optical microscope also show that the sound and aged specimen, sliding phenomenon by shear and thermal stress is observed both interface between mica and epoxy.

정보화시대의 공동체 : 정보화의 진전과 기업·소비자 관계 변화를 중심으로

이대식,김성국,김영삼,신종국,임정덕,조영복,조정문 釜山大學校 商科大學 2000 釜山商大論集 Vol.71 No.-

In this study, we questioned about the possibility of the formation of the new community like relationship at the market on the contrary to the traditional market relationship. We conjectured that kind of new community like relationship may possibly arise because of wide spread of new information technology at market behavior of both consumer and producer side. Traditional buyer-seller relationship was characterized as an anonymous one-shot relationship, however, recent changes were mainly emphasized the rich mutual interactions from both sides. Producers needs more consumer side informations as well as consumers begin to participate on production side at more organized level. Some theoretical studies predict that more rich social relationship will appear at the market since the adoption and wide spread of new information technology. We assume that main functions and ingredients of the community are first, the coordination mechanism which reduces conflicts among community members and second, functional efficiency which meets the common needs of community and third, communicational rationality which enhances the integrity of community members. We surveyed about 100 small and medium sized firms located at Pusan and Kyung-Nam area in order to find out whether firms have community like relationship with their consumers. We fine that most of firms realize the impotance of consumer networks, however most of firms consider this kind of network as a marketing strategy and a source of product design instead of more community oriented characteristics like mutual trust building and social interactions.

상악 전돌증 환자의 치험례

이진하,김진수,김성국 慶北大學校 齒科大學 1990 慶北齒大論文集 Vol.7 No.2

This is a case report of both superior(5mm) & posterior (7mm) repositioning of maxilla. This patient, 25-year old female, was admitted to our Dept. of Oral & Maxillofacial Surgery with chief complaint of lip incompetence & maxillary prognathism after orthodontic treatment. In this case, we performed Le fort Ⅰ osteotomy and ostectomy of maxillary tuberosity region including the perpendicular plate of the palatine bone. This repositioned maxilla is fixed with miniplate and wedge-shaped corticocancellous bone blocks are grafted between the margins of the upper & lower segments. As a result, we achieved improving appearance & lip incompetence.

日本企業의 雇傭管理에 關한 考察

李經國,元性權 明知大學校 附設 日本問題硏究所 1997 日本硏究 Vol.5 No.-

태권도선수의 체중감량시 補中治濕湯 투여가 신체조성, 혈액생화학적 변인 및 호르몬 변화에 미치는 영향

이승국,문원재,오재근 韓國體育大學校 1997 論文集 Vol.20 No.-

This study was to investigate the effects of Bo-Jung-Chi-Seub-Tang administration on body composition, blood biochemical factors and hormonal changes during 2 weeks Weight Reduction in TaeKwonDo Athletes. 10 subjects were belong to be in elite athletes of KNUPE. They were divided two groups(administration group and non-administration) by 5 subjects in each group randomly. As the results of the experiment, conclusions were made as follows: In terms of % Fat, Fat W.T., Mg, TG, Renin, it was shown significant differences between two groups. Although there were no significant differences, rate of weight reduction and levels of LBM, BUN, total cholesterol, HDL-C, LDL-C in Bo-Jung-Chi-Seub-Tang administration group were changed more higher or lower than non-administration group.

리더십기능이 체육학습에 미치는 효과

이상연,육조영,이성국 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of this study was to investigate the relationship between teacher's leadership and task environment recognized by learners and the effect of leadership of sub-group in the class upon organizational behavior. It meant to examine the instructional activity and learning activity from the standpoint of leadership theory. The subjects 139 pupils. 1. Teacher's leadership of task orentation decreased uncertainty of expectancy which conditioned task environment. 2. Teacher's leadership of human orientation and task orientation enhanced valence of learning activity and outcome. 3. Significant positive correlation between sub-group's leadership human orientation and learning effectiveness in favorable task environment for learners were found(p<0.1). 4. Significant positive correlation between sub-group sleadership of human orientation (p<.01) and task orientation (p<.05) and recognition of pleasure in sport were found in favorable task environment

21C 부산체육의 활성화를 위한 시설 및 프로그램 연구

이상봉,김용재,서국웅,양점홍 釜山大學校 附設 體育科學硏究所 1996 體育科學硏究所 論文集 Vol.12 No.-

This research investigated Institution and Program for the Development of the Pusan Sports in 21C by examining literatures. Conclusiona from the results as follows. 1. We should provide a long period propulsion project in three stage with planning, diffusion and development stage for the bring development and diffusion of Program. 2. We should establish a specialty research institution, the exclusive responsibility of program's development and research. 3. We should provide various participation programs not that for an elite athlete but that for all nation. 4. Enlarge the same taste club and we must support with various administration and financial. 5. Cultivate a specialty leader and we must take interested in the study and training with continual.

신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.