부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

충북지역 생산단계 수삼 중 잔류농약 모니터링 및 위해성 평가(2019)

송태화 ( Tae Hwa Song ),이영욱 ( Young Wook Lee ),윤택한 ( Taek Han Youn ),박은아 ( Eun A Park ),심은선 ( Eun Sun Shim ),이주희 ( Ju Hee Lee ),경기성 ( Kee Sung Kyung ) 한국환경농학회 2021 한국환경농학회지 Vol.40 No.2

BACKGROUND: It is very important to monitor the residual characteristics of pesticides in pre-harvest fresh ginseng to ensure consumer safety. METHODS AND RESULTS: Forty-eight fresh ginseng samples were collected from 8 ginseng-growing fields 10 days before harvest and pesticide residues in fresh ginseng with and without rhizome (head of ginseng) and rhizome were analyzed for 320 pesticides by using GC-MS/MS and LC-MS/MS. As a result of the pesticide residue analysis, the number of pesticides detected from the fresh ginseng with rhizome, that without rhizome and rhizome were 26, 25, and 40, respectively, indicating that high number of pesticides found in rhizome, compared with the other parts. Pyraclostrobin was detected with the highest frequency in all samples, reaching to 21.2% in fresh ginseng with rhizome, 16.8% in that without rhizome, and 14.8% in rhizome. CONCLUSION: The residue levels of pesticides detected did not exceed their maximum residue limits, in spite of residual data in fresh ginseng before harvest. The amounts of the estimated daily intakes of all the detected pesticides were found to be from 0.018 to 1.818% of their acceptable daily intakes, indicating that concentrations of pesticides detected from fresh ginseng with and without rhizome collected before harvest do not pose the immediate health risks.

Cu₃Si-doped porous-silicon particles prepared by simplified chemical vapor deposition method as anode material for high-rate and long-cycle lithium-ion batteries

Woo, Jae-Young,Kim, A-Young,Kim, Min Kyu,Lee, Sang-Hyup,Sun, Yang-Kook,Liu, Guicheng,Lee, Joong Kee ELSEVIER SCIENCE 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.701 No.-

<P><B>Abstract</B></P> <P>To provide a possible proposal for the large-scale production of a high performance silicon-based anode material in the lithium battery industry, a Cu<SUB>3</SUB>Si nanoparticle doped porous-silicon particles was prepared via a simplified chemical vapor deposition (CVD) process and heat treatment for the first time. In this work, the Cu<SUB>3</SUB>Si doping content was optimized by discharge/charge, transmission electron microscopy and electrochemical impedance spectroscopy tests. The results show that compared with the porous-silicon (PS) particles, the Cu<SUB>3</SUB>Si doping significantly enhanced the discharge capacity, coulombic efficiency, capacity retention, and high-rate performance of the silicon-based anode. The optimum performance with a discharge capacity of 3036.4 mA h g<SUP>−1</SUP> and a coulombic efficiency of 90.49% at the first cycle (after the first three formation cycles) and a capacity retention of 58.72% after 100 cycles occurred at a Cu<SUB>3</SUB>Si doping content of 2 wt%. The reasons for this are as follows: the PS particles with a similarly silicon nanorod structure accommodated the volume change to maintain the mechanical stability of the electrode during the cycling process; during the simplified CVD process, the nanostructure of silicon was retained; the high conductivity due to Cu<SUB>3</SUB>Si doping decreased the formation resistance of the solid-electrolyte interphase (SEI) film and enhanced the diffusion coefficient of Li<SUP>+</SUP> inside the silicon-based material; both fewer Cu<SUB>3</SUB>Si doping and aggregation particles resulting from excessive Cu<SUB>3</SUB>Si doping yielded insufficient electrical conductivity and decreased the formation resistance of the SEI film for the silicon-based material.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using simplified CVD process for promoting mass production of Cu<SUB>3</SUB>Si doped porous Si material. </LI> <LI> Si nanorods accommodate volume change to maintain stability of anode during cycling. </LI> <LI> High conductivity caused by Cu<SUB>3</SUB>Si doping decreases formation resistance of SEI film. </LI> <LI> PS with uniform 2 wt% Cu<SUB>3</SUB>Si doping possesses the highest performance. </LI> </UL> </P>

Allelic Variation of Glutenin, Granule-Bound Starch Synthase I and Puroindoline in Korean Wheat Cultivar

Chul Soo Park,Roberto J. Peña,Byung-Kee Baik,Chon-Sik Kang,Hwa-Young Heo,Young-keun Cheong,Sun-Hee Woo 韓國作物學會 2009 Korean journal of crop science Vol.54 No.2

To investigate the genetic variation of high-and low-molecular-weight glutenin subunits (BMW-GS and LMW-GS), granule-bound starch synthase I (GBSSI) and puroindoline in 24 Korean wheat cultivars. At the BMW-GS compositions, three Glu-A1 alleles, five Glu-B1 alleles and three Glu-D1 alleles were identified. The high frequency of alleles at each locus was Glu-A1c allele (15 cultivars), Glu-B1b allele (16 cultivars) and Glu-D1f allele (16 cultivars). Four alleles were identified at the Glu-A3 and Glu-B3 loci and three at Glu-D3 locus and Glu-A3d, Glu-B3d and Glu-D3a were mainly found at each Glu-3 locus. Glu-A3d, Glu-B3d, Glu-D3b or c (4 cultivars, respectively) and Glu-A3d, Glu-B3d, Glu-D3a and Glu-A3c, Glu-B3d or h, Glu-D3a (3 cultivar, respectively) were predominantly found in Korean wheats. At the GBSS compositions, 2 waxy wheat cultivars, Shinmichal and Shinmichal1, showed null alleles on the Wx loci and other cultivars were wild type in GBSS compositions. At the puroindoline gene compositions, Korean wheat cultivars carried 3 genotypes, which 10 cultivars (41.7%) were Pina-D1a and Pinb-D1a, 11 cultivars (45.8%) had Pina-D1a and Pinb-D1b and 3 cultivars (12.5%) carried Pina-D1b and Pinb-D1a. These genetic variations could present the information to improve flour and end-use quality in Korean wheat breeding programs.

30 ∼ 40대 한국여성이 선호하는 피부색에 대한 연구

강내규 ( Nae Gyu Kang ),곽택종 ( Taek Jong Kwak ),김정아 ( Jung A Kim ),김태훈 ( Tae Hoon Kim ),문태기 ( Tae Kee Moon ),박선규 ( Sun Gyoo Park ),송다영 ( Da Young Song ),이수진 ( Su Jin Lee ),장민열 ( Min Youl Chang ),장희정 ( Hee 대한화장품학회 2014 대한화장품학회지 Vol.40 No.4

피부는 인체의 외부를 덮고 있는 기관일 뿐 아니라, 체온을 조절하고, 감각기능을 수행하며, 약물을 투입하는 경로가 되기도 하고 내부 기관의 이상을 표현하는 기관이 되기도 한다. 이러한 이유로 인해, 사람들은 타인의 얼굴 피부의 상태를 보고 건강의 이상 징후를 감지하거나, “안색이 좋다”, “생기가 있다” 등으로 신체의 컨디션을 표현해왔다. 환경과 노화정도에 따라서 피부색, 피부결, 수분, 탄력 등이 달라지는 것도 알려진 사실이다. 특히, 피부색은 아름다움, 매력도와 같은 주관적인 인상을 판단하는데 영향을 미치는 인자로 작용하는데, 화장품이나 피부시술을 통해 피부색을 개선하고자 하는 소비자를 통해서 그 수요를 짐작할 수 있다. 하지만 피부색이 여러 색의 구성임에도 불구하고, 대부분의 화장품이 하얀 피부로 개선해주는 것에 초점을 맞추고 있으며 여성들이 선호하는 피부색에 대한 연구가 아직까지는 미흡한 실정이다. 본 연구에서는 30 ∼ 40대의 한국여성을 대상으로 같은 피부 밝기에서 붉은 정도와 푸른 정도가 달리 주어진 선호도 조사를 통해 긍정적으로 인식하는 피부색의 상태를 발견하였다. 한국여성은 실제 평균 피부색에 비해 붉은색과 푸른색이 높은 피부를 선호하는 것으로 나타났으며, 피부에 대한 표현을 달리하였을 때에도 경향은 유사하였다. 본 연구 결과는 새로운 소비자의 요구를 만족시키는 제품 개발에 활용될 수 있을 것이라고 생각한다. The skin is an organ that covers the outside of the human body. And it regulates body temperature, performs sensory function, deliveries the drug, and represents the abnormality of the internal organs. For these reasons, people have been expressing the condition of the body based on the state of the skin of the face to detect sign of health, “you look well”, “face with vitality”. Depending on the degree of aging and the environment, it is a fact that skin color, skin texture, moisture and firmness could be different. In particular, the color of the skin acts as a factor that affects to determine the subjective impression such as beauty and charm. We could expect consumer’s demand through the market of whitening cosmetics and skin treatment. However, existing most of the cosmetics focus on improving whiteness. The number of studies that focused on skin color is not enough. In this study, we found out the skin color condition by carrying out surveys of 100 women between 30s and 40s. Our study would be very useful to develop new cosmetic products and to satisfy customer’s needs.

비전형적인 세침흡인 세포학적 소견을 보인 후복막강 부신경절종 - 1례 보고 -

김진아,김영신,강창석,이안희,김병기,심상인,김선무,Kim, Jean-A,Kim, Young-Shin,Kang, Chang-Suk,Lee, An-Hi,Kim, Byung-Kee,Shim, Sang-In,Kim, Sun-Moo 대한세포병리학회 1994 대한세포병리학회지 Vol.5 No.1

A case of retroperitoneal paraganglioma is presented with fine needle aspiration cytologic features. A 57-year-old woman complained of abdominal discomfort and left flank pain for 2 years. The abdominal CT scan revealed an ovoid mass left to the abdominal aorta. Percutaneous fine needle aspiration was performed from the mass. The smear revealed cellular sheets or groups on hemorrhagic background. The tumor cells were ovoid, round to spindle shaped with mild to moderate cellular pleomorphism. The nuclei were round to ovoid and had evenly dispersed chromatin and small nucleoli. The cytoplasm was amphophilic, finely granular and poorly defined. Cells having large or spindle nuclei were quite frequently observed, however, mitosis was not present The cytologic findings suggested paraganglioma, but the frequent spindle cell pattern and the pleomorphism made it difficult to exclude other endocrine tumors and sarcomas. The clinical, histological and ultrastructural findings as well as cytologic findings contributed to confirmatory diagnosis.

Risk factors for cytological progression in HPV 16 infected women with ASC-US or LSIL: The Korean HPV cohort

( Kyeong A So ),( Seon Ah Kim ),( Yoo Kyung Lee ),( In Ho Lee ),( Ki Heon Lee ),( Jee Eun Rhee ),( Mee Kyung Kee ),( Chi Heum Cho ),( Sung Ran Hong ),( Chang Sun Hwang ),( Mi Seon Jeong ),( Ki Tae Kim 대한산부인과학회 2018 Obstetrics & Gynecology Science Vol.61 No.6

Objective This study was to identify the risk factors for cytological progression in women with atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesions (LSIL). Methods We analyzed data from women infected with the human papillomavirus (HPV) who participated in the Korean HPV cohort study. The cohort recruited women aged 20-60 years with abnormal cervical cytology (ASC-US or LSIL) from April 2010. All women were followed-up at every 6-month intervals with cervical cytology and HPV DNA testing. Results Of the 1,158 women included, 654 (56.5%) and 504 (43.5%) women showed ASC-US and LSIL, respectively. At the time of enrollment, 143 women tested positive for HPV 16 (85 single and 58 multiple infections). Cervical cytology performed in the HPV 16-positive women showed progression in 27%, no change in 23%, and regression in 50% of the women at the six-month follow-up. The progression rate associated with HPV 16 infection was higher than that with infection caused by other HPV types (relative risk [RR], 1.75; 95% confidence interval [CI], 1.08-2.84; P =0.028). The cytological progression rate in women with persistent HPV 16 infection was higher than that in women with incidental or cleared infections (P <0.001). Logistic regression analysis showed a significant relationship between cigarette smoking and cytological progression (RR, 4.15; 95% CI, 1.01-17.00). Conclusion The cytological progression rate in HPV 16-positive women with ASC-US or LSIL is higher than that in women infected with other HPV types. Additionally, cigarette smoking may play a role in cytological progression.

Detection of Hepatitis B Virus and Mycobacterium tuberculosis in Korean Dental Patients

Lee, Sun-A,Yoo, So Young,Kay, Kee-Sung,Kook, Joong-Ki The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.3

This study examined the detection rate of the hepatitis B virus (HBV) and Mycobacterium tuberculosis (Mtb) in serum and saliva samples, respectively, from 120 dental patients who were unaware if they have or had either hepatitis or tuberculosis. The frequencies of HBsAg and anti-HBs were determined using an immunochromatic assay. Mtb positivity was determined by the PCR method. Of the 120 patients, 7 (5.8%) were HBV positive and 30 (25.0%) were Mtb positive. This highlights the fact that dental health care workers (DHCWs) can be exposed to the risk of infection from blood- or saliva-borne pathogens as a consequence of their work. Therefore, it is very important to prevent cross infection between patients and dental personnel. Accordingly, laboratory tests prior to surgical treatment are needed to determine the infectious state of dental patients in order to prevent the transmission of infectious diseases in dental clinics.

Influence of personality on depression, burden, and health-related quality of life in family caregivers of persons with dementia

Kim, Sun Kyung,Park, Myonghwa,Lee, Yunhwan,Choi, Seong Hye,Moon, So Young,Seo, Sang Won,Park, Kyung Won,Ku, Bon D.,Han, Hyun Jeong,Park, Kee Hyung,Han, Seol-Heui,Kim, Eun-Joo,Lee, Jae-Hong,Park, Sun A Cambridge University Press 2017 INTERNATIONAL PSYCHOGERIATRICS - Vol.29 No.2

<B>ABSTRACT</B><B>Background:</B><P>Personality may predispose family caregivers to experience caregiving differently in similar situations and influence the outcomes of caregiving. A limited body of research has examined the role of some personality traits for health-related quality of life (HRQoL) among family caregivers of persons with dementia (PWD) in relation to burden and depression.</P><B>Methods:</B><P>Data from a large clinic-based national study in South Korea, the Caregivers of Alzheimer's Disease Research (CARE), were analyzed (<I>N</I> = 476). Path analysis was performed to explore the association between family caregivers’ personality traits and HRQoL. With depression and burden as mediating factors, direct and indirect associations between five personality traits and HRQoL of family caregivers were examined.</P><B>Results:</B><P>Results demonstrated the mediating role of caregiver burden and depression in linking two personality traits (neuroticism and extraversion) and HRQoL. Neuroticism and extraversion directly and indirectly influenced the mental HRQoL of caregivers. Neuroticism and extraversion only indirectly influenced their physical HRQoL. Neuroticism increased the caregiver's depression, whereas extraversion decreased it. Neuroticism only was mediated by burden to influence depression and mental and physical HRQoL.</P><B>Conclusions:</B><P>Personality traits can influence caregiving outcomes and be viewed as an individual resource of the caregiver. A family caregiver's personality characteristics need to be assessed for tailoring support programs to get the optimal benefits from caregiver interventions.</P>

Case report : Solitary Synchronous Metastatic Gastric Cancer Arising from T1b Renal Cell Carcinoma: A Case Report and Systematic Review

( Mi Young Kim ),( Hwoon Yong Jung ),( Kee Don Choi ),( Ho June Song ),( Jeong Hoon Lee ),( Do Hoon Kim ),( Kwi Sook Choi ),( Sun A Kim ),( Gin Hyug Lee ),( Jin Ho Kim ) The Editorial Office of Gut and Liver 2012 Gut and Liver Vol.6 No.3

Metastasis to the stomach from renal cell carcinoma (RCC) is extremely rare. Usually, gastric metastasis seems to be a late event in patients with RCC and is accompanied by disseminated tumor spread to other organs. Solitary syn-chronous gastric metastasis from small, localized RCC has rarely been reported. We report a case of 79-year-old man with synchronous gastric metastasis presenting with a single erosive lesion from pT1 RCC. The patient underwent radical nephrectomy and endoscopic resection for metastatic gastric cancer. The resected specimen showed an ill-defined tumor, approximately 0.6 cm long, with a clear resection margin. The morphologic features of the tumor cells were consis-tent with those of metastatic RCC of the clear cell type. At 6 months`s follow-up, the patient did not show local recurrence or additional metastasis on upper endoscopy and computed tomography scan. (Gut Liver 2012;6:388-394)