대장통과시간 측정용 방사선 비투과성 표지자인 KolomarkTM의 국내 개발

김지은,이풍렬,김영호,성인경,심상군,김재준,고광철,최규완,임효근,서수원 대한소화관운동학회 1999 Journal of Neurogastroenterology and Motility (JNM Vol.5 No.2

Amino Acids Important for DNA Recognition by the Response Regulator OmpR

Rhee, Jee Eun,Sheng, Wanyun,Morgan, Leslie K.,Nolet, Ryan,Liao, Xiubei,Kenney, Linda J. American Society for Biochemistry and Molecular Bi 2008 The Journal of biological chemistry Vol.283 No.13

<P>Response regulators undergo regulated phosphorylation and dephosphorylation at conserved aspartic acid residues in bacterial signal transduction systems. OmpR is a winged helix-turnhelix DNA-binding protein that functions as a global regulator in bacteria and is also important in pathogenesis. A detailed mechanistic picture of how OmpR binds to DNA and activates transcription is lacking. We used NMR spectroscopy to solve the solution structure of the C-terminal domain of OmpR (OmpR(C)) and to analyze the chemical shift changes that occur upon DNA binding. There is little overlap in the interaction surface with residues of PhoB that were reportedly involved in protein/protein interactions in its head-to-tail dimer. Multiple factors account for the lack of overlap. One is that the spacing between the OmpR half-sites is shorter than observed with PhoB, requiring the arrangement of the two OmpR molecules to be different from that of the PhoB dimer on DNA. A second is the demonstration herein that OmpR can bind to its high affinity site as a monomer. As a result, OmpR(C) appears to be capable of adopting alternative orientations depending on the precise base composition of the binding site, which also contributes to the lack of overlap. In the presence of DNA, chemical shift changes occur in OmpR in the recognition alpha-helix 3, the loop between beta-strand 4 and alpha-helix 1, and the loop between beta-strands 5 and 6. DNA contact residues are Val(203) (T), Arg(207) (G), and Arg(209) (phosphate backbone). Our results suggest that OmpR binds to DNA as a monomer and then forms a symmetric or asymmetric dimer, depending on the binding site. We propose that during activation OmpR binds to DNA and undergoes a conformational change that promotes phosphorylation of the N-terminal receiver domain, the receiver domains dimerize, and then the second monomer binds to DNA. The flexible linker of OmpR enables the second monomer to bind in multiple orientations (head-to-tail and head-to-head), depending on the specific DNA contacts.</P>

Notes : Identification of the Vibrio vulnificus cadC and Evaluation of Its Role in Acid Tolerance

( Jee Eun Rhee ),( Hyun Mok Ju ),( U Ryung Park ),( Byoung Chul Park ),( Sang Ho Choi ) 한국미생물 · 생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.5

AphB Influences Acid Tolerance of Vibrio vulnificus by Activating Expression of the Positive Regulator CadC

Rhee, Jee Eun,Jeong, Hee Gon,Lee, Jeong Hyun,Choi, Sang Ho American Society for Microbiology 2006 Journal of Bacteriology Vol.188 No.18

<B>ABSTRACT</B><P>A mutant of <I>Vibrio vulnificus</I> that was more sensitive to low pH was screened from a library of mutants constructed by random transposon mutagenesis. By use of a transposon-tagging method, an open reading frame encoding a LysR homologue, AphB, was identified and cloned from <I>V. vulnificus</I>. The deduced amino acid sequence of AphB from <I>V. vulnificus</I> was 80% identical to that reported from <I>V. cholerae</I>. A mutational analysis demonstrated that the gene product of <I>aphB</I> contributes to acid tolerance of <I>V. vulnificus</I>. The lysine decarboxylase activity and cellular level of the <I>cadA</I> transcript were decreased in the <I>aphB</I> mutant, indicating that AphB exerts its effect on the acid tolerance of <I>V. vulnificus</I> by enhancing the expression of <I>cadBA</I>. Western blot analyses demonstrated that the cellular level of CadC, a transcription activator of the <I>cadBA</I> operon, was significantly reduced by <I>aphB</I> mutation, and a primer extension analysis revealed that the <I>cadC</I> promoter (P<I>cadC</I>) activity was under the positive control of AphB. A direct interaction between AphB and the P<I>cadC</I> DNA was demonstrated by gel mobility shift assays. The AphB binding site mapped by deletion analyses of the P<I>cadC</I> regulatory region and confirmed by a DNase I protection assay was centered at the 61.5 bp upstream of the transcription start site. Accordingly, these results demonstrate that AphB and CadC function sequentially in a regulatory cascade to activate <I>cadBA</I> expression and that AphB activates the expression of <I>cadC</I> by directly binding to an upstream region of P<I>cadC</I>.</P>

Activation of the Vibrio vulnificus cadBA Operon by Leucine-Responsive Regulatory Protein is Mediated by CadC

( Jee Eun Rhee ),( Kun Soo Kim ),( Sang Ho Choi ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.11

Evidence That Temporally Alternative Expression of the Vibrio vulnificus Elastase Prevents Proteolytic Inactivation of Hemolysin

RHEE, JEE EUN,LEE, JEONG HYUN,JEONG, HYE SOOK,PARK, URYUNG,LEE, DONG-HA,WOO, GUN-JO,MIYOSHI, SHIN-ICHI,CHOI, SANG-HO 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.6

Numerous secreted and cell-associated virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. Among the putative virulence factors are an elastase, elastolytic protease, and a cytolytic hemolysin. Effects of the elastase on the hemolysin were assessed by evaluating changes of hemolytic activities either in the presence or absence of the protease. Although hemolytic activity in the culture supernatant was lowered by the purified elastase added in Litro, the cellular level of hemolytic activity was unaffected by the mutation of vvpE encoding the elastase. Growth kinetic studies revealed that hemolysin reached its maximum level in the exponential phase of growth, and the elastase appeared at the onset of the stationary phase. These results have provided insight into the regulation of virulence factors: temporally coordinate regulation of virulence factors is essential for the overall success of the pathogen during pathogenesis.

Dynamics of Genomic, Epigenomic, and Transcriptomic Aberrations during Stepwise Hepatocarcinogenesis

Jee, Byul A,Choi, Ji-Hye,Rhee, Hyungjin,Yoon, Sarah,Kwon, So Mee,Nahm, Ji Hae,Yoo, Jeong Eun,Jeon, Youngsic,Choi, Gi Hong,Woo, Hyun Goo,Park, Young Nyun American Association for Cancer Research 2019 Cancer Research Vol.79 No.21

<P>Multiomics profiling and integrative analyses of stepwise hepatocarcinogenesis reveal novel mechanistic and clinical insights into hepatocarcinogenesis.</P><P><B></B></P><P>Hepatocellular carcinoma (HCC) undergoes a stepwise progression from liver cirrhosis to low-grade dysplastic nodule (LGDN), high-grade dysplastic nodule (HGDN), early HCC (eHCC), and progressed HCC (pHCC). Here, we profiled multilayered genomic, epigenomic, and transcriptomic aberrations in the stepwise hepatocarcinogenesis. Initial DNA methylation was observed in eHCC (e.g., <I>DKK3, SALL3</I>, and <I>SOX1</I>) while more extensive methylation was observed in pHCC. In addition, eHCCs showed an initial loss of DNA copy numbers of tumor suppressor genes in the 4q and 13q regions, thereby conferring survival benefits to cancer cells. Transcriptome analysis revealed that HGDNs expressed endoplasmic reticulum (ER) stress–related genes, while eHCC started to express oncogenes. Furthermore, integrative analysis indicated that expression of the serine peptidase inhibitor, Kazal type 1 (SPINK1), played a pivotal role in eHCC development. Significant demethylation of SPINK1 was observed in eHCC compared to HGDN. The study also demonstrated that ER stress may induce SPINK1 demethylation and expression in liver cancer cells. In conclusion, these results reveal the dynamics of multiomic aberrations during malignant conversion of liver cancer, thus providing novel pathobiological insights into hepatocarcinogenesis.</P><P><B>Significance:</B></P><P>Multiomics profiling and integrative analyses of stepwise hepatocarcinogenesis reveal novel mechanistic and clinical insights into hepatocarcinogenesis.</P>

한국 여성의 정상 임신과 임신성 당 대사 이상에서 아디포카인의 변화

오은숙 ( Eun Suk Oh ),한정희 ( Jung Hee Han ),한성민 ( Sung Min Han ),임지애 ( Jee Aee Im ),이은정 ( Eun Jung Rhee ),박철영 ( Cheol Young Park ),오기원 ( Ki Won Oh ),이원영 ( Won Young Lee ) 대한당뇨병학회 2009 Diabetes and Metabolism Journal Vol.33 No.4

연구배경: 정상 내당능을 가진 임신 3분기 초기의 임신부의 혈중 아디포카인의 변화를 비임신군과 비교하여 알아보고, 정상 내당능을 가진 임신부군(NGT), 임신성 내당능 장애군(GIGT), 및 임신성 당뇨병군(GDM) 간의 혈중 아디포카인 농도의 차이를 알아보았다. 또한 임신 중 당 대사 이상 발생에 영향을 미치는 인자를 살펴보고, 혈중 아디포카인의 농도에 영향을 미치는 인자들도 알아보았다. 방법: 임신 24~28주에 시행한 100 g 경구 당 부하 검사결과에 따라 대상군을 (1) NGT군(n=40), (2) GIGT군(n=45), (3) GDM군(n=44)으로 나누었다. 비임신 대조군으로는 NGT군과 임신 전 Body mass index (BMI)와 나이가 일치되는 여성(n=41)으로 하였다. 혈중 아디포넥틴, 렙틴, 레지스틴은 Eenzyme-linked immunosorbent assay로 측정하였다. 공복 포도당, 지질 농도, 인슐린, hs-CRP를 측정하였다. 결과: 임신 24~28주에 정상 내당능을 가진 임신부는 비임신군에 비하여, 혈중 아디포넥틴은 감소되고, 렙틴은 증가되었으며 hs-CRP도 증가하였다. 세 군의 임신부들의 혈중 아디포카인의 농도를 비교한 결과, 레지스틴은 GDM군과 GIGT군에서 NGT군에 비해 의미있게 증가되었다. 하지만 혈중 아디포넥틴과 렙틴은 세 군 간 의미있는 차이를 관찰할 수 없었다. 임신 중 당 대사 이상의 발생에 기여하는 인자는 레지스틴과 공복 혈당이었다. 임신 중 혈중 아디포넥틴 농도는 고밀도 콜레스테롤과 양의 상관관계, 렙틴, BMI, 중성지방, 공복 인슐린, HOMA-IR, 중성지방/고밀도 콜레스테롤 비와 음의 상관관계를 보였다. 혈중 렙틴 농도는 BMI, 공복 혈당 및 인슐린, HOMA-IR, HOMA2%B, hs-CRP 및 당화혈색소와 양의 상관관계, 나이 및 아디포넥틴 농도와 음의 상관관계를 보였다. 혈중 레지스틴 농도는 100 g 당 부하 검사 동안의 Area under the curve for glucose (AUCG) 및 당 부하 후 1, 2, 3시간 후 혈당과 양의 상관관계, 고밀도 콜레스테롤과 음의 상관관계가 있었다. 다중회귀분석에서 100 g 당 부하 후 2시간째 혈당이 레지스틴 농도의 의미있는 예측인자였다. 결론: 임신 24~28주에 NGT를 가진 임신부에서 나이와 BMI가 일치된 비임신군에 비하여 혈중 아디포넥틴은 감소하고, 렙틴은 증가되었다. GDM군과 GIGT군에서 NGT군에 비해 의미있게 혈중 레지스틴 농도가 증가되었으며, 레지스틴의 증가는 임신 중 당 대사 이상의 발생의 주요 결정인자였다. 또한 레지스틴 농도는 당 부하 후 AUCG 및 경구 당 부하 검사 1, 2, 3시간째 혈당과 양의 상관관계가 있었다. 따라서 한국여성의 임신 중 당 대사 이상의 발생에 레지스틴의 역할이 중요하리라 여겨진다. Background: The aims of this study were to compare adipokine concentrations of pregnant women in the 24th~28th weeks of gestation to those of non-pregnant women. We compared the concentrations of adipokines in women with gestational diabetes mellitus (GDM), gestational impaired glucose tolerance (GIGT) and normal glucose tolerance (NGT). We also investigated the role of adipokines in the development of gestational glucose intolerance. Methods: We surveyed 129 pregnant women who underwent a 100 g oral glucose tolerance test (OGTT) during the 24th~28th weeks of gestation. Participants were classified into three groups: (1) NGT (n=40), (2) GIGT (n=45), and (3) GDM (n=44). Pregnant subjects with NGT were matched to non-pregnant controls for BMI and age (n=41). Results: Pregnant women with NGT exhibited significantly decreased adiponectin levels and elevated leptin levels compared to non-pregnant controls. Mean plasma resistin levels were significantly higher in women with GDM and GIGT than in women with NGT. Resistin and fasting glucose were significant predictors for the development of gestational glucose intolerance. Conclusion: Plasma adiponectin levels were decreased and leptin levels were increased in pregnant subjects with NGT compared to BMI and age matched non-pregnant controls. Women with GDM and GIGT exhibit significantly elevated concentrations of resistin compared with women with NGT. Increased resistin levels were also associated with the development of gestational glucose intolerance. Resistin may play an important role on the development of gestational glucose intolerance in Korean women. (Korean Diabetes J 33:279-288, 2009)

Profiling of Glucosinolate Contents in Roots and leaves of Radish (Raphanus sativus var. sativus)

Jae-eun Lee,Ju-Hee Rhee,Young Jee Kim,Awraris Derbie Assefa,Jaejong Noh,Ho-Sun Lee,Jung-Sook Sung,On-Sook Hur,Na-Young Ro,Aejin Hwang 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10

국내에서 개발된 대장통과시간 측정용 방사선 비투과성 표지자의 유용성

김지은(Jee Eun Kim),이풍렬(Poong Lyul Rhee),김영호(Young Ho Kim),성인경(In Kyung Sung),박동일(Dong Il Park),현재근(Jae Geun Hyun),이준행(Jun Haeng Lee),손희정(Hee Jung Son),김재준(Jae Jun Kim),고광철(Kwang Cheol Koh),백승운(Seung Woo 대한내과학회 2001 대한내과학회지 Vol.60 No.4

N/A Background: Among various methods for measuring colon transit time, radio-opaque marker study is simple, reproducible and economical method. The commonly used marker, Sitzmarks? (Konsyl Pharmaceuticals Inc. Texas) had limitation in it s use due to expensiveness and difficulty in importation. We thought that the new domestic marker comparable to Sitzmarks is necessary and made a Kolomark (Korean colon marker )TM. The comparison of radio-opaqueness and the measurement of colon transit times by two markers were done. Methods: In two 1000 ml beakers, 350 ml of rice-gruel, sever al chicken-bones and ten rings of Sitzmarks? and KolomarkTM were mixed separately. Then, X-ray films of the two beakers were taken. The digital image file was analyzed by Image and the value of pixels were obtained. These were repeated five times. Colon transit times were measured in 60 healthy persons stratified by age, 30 by Sitzmarks? and 30 by KolomarkTM. Results: The mean value of pixel of KolomarkTM was much lower than that of Sitzmarks?. The difference between background or beaker and KolomarkTM was much greater than that of Sitzmarks?. There was no significant difference between colon transit time studied by Sitzmarks? and KolomarkTM. Conclusion: KolomarkTM, a more radio-opaque and cheaper marker than Sitzmarks? will be applied usefully for measuring colon transit time.(Korean J Med 60: 337- 341, 2001)