White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

Zhu Qinchao,Zhou Xuhao,Zhang Yanan,Ye Di,Yu Kang,Cao Wangbei,Zhang Liwen,Zheng Houwei,Sun Ziyang,Guo Chengchen,Hong Xiaoqian,Zhu Yang,Zhang Yajun,Xiao Ying,Valencak Teresa G.,Ren Tanchen,Ren Daxi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background : Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods : A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result : Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion : Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Sun Di,Mou Shan,Chen Lifeng,Yang Jie,Wang Rongrong,Zhong Aimei,Wang Wei,Tong Jing,Wang Zhenxing,Sun Jiaming 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been found to have a great potential for soft tissue repair due to various biological functions, including pro-angiogenesis and low immunogenicity. However, the low yield and heterogeneity of MSC-EVs limited their clinical transformation. This study was designed to develop a novel adipose-derived stem cell engineered nanovesicles (ADSC-NVs) with high production and explore its pro-angiogenetic effect and application in adipose tissue regeneration. Methods: Adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) were isolated from an EVs-free culture medium for human ADSCs (hADSCs). ADSC-NVs were prepared by sequentially extruding ADSCs followed by iodixanol density gradient ultracentrifugation and were compared with ADSC-EVs in morphology, size distribution, protein contents and yield. The pro-angiogenetic effect of ADSC-NVs in different doses (0, 5, 20 and 80 μg/mL) in vitro was determined using transwell assay, tube formation assay, western blot and qRT-PCR. In vivo, BALB/c nude mice were administered injection of a mixture of fat granules and different dose of ADSC-NVs and grafts were harvested at 12 weeks post-transplantation for further analysis. By analyzing the weight and volume of grafts and histological evaluation, we investigated the effect of ADSC-NVs in vessel formation and adipose tissue regeneration. Results: Our results showed yield of purified ADSC-NVs was approximately 20 times more than that of ADSC-EVs secreted by the same number of ADSCs. In vitro, both ADSC-NVs and ADSC-EVs exhibited a dose-dependent proangiogenetic effect, despite their distinct miRNA profiles. These effects of ADSC-NVs may be mediated by enriched miR-21-5p via PTEN inhibition and PI3K/p-Akt signaling activation. Furthermore, after a mixed injection of ADSC-NVs, vessel formation and adipose regeneration were observed in vivo in fat implants. Conclusions: Our study developed a potent alternative of ADSC-EVs. ADSC-NVs have a high pro-angiogenesis potential and can be used as cell-free therapeutic biomaterials in soft tissue regeneration.

Building a model to identify factors affecting consumers’ purchase intention online

Di Sun,Euiho Suh,Suchul Lee 대한산업공학회 2012 대한산업공학회 추계학술대회논문집 Vol.2012 No.11

In order to explore how consumers’ purchase intention online can be affected, this research proposed three major factors: consumer related factors, consumer-website interaction related factors, and website related factors as a new dimension, based on the relationships between consumers and website. The composing constructs of consumer related factors are impulse purchase, subjective norm, and attention to social comparison information, while for website related factors, the model considers four type of variables, namely, perceived product value, perceived user interface quality, perceived security quality, and perceived delivery quality. Consumerwebsite interaction factors consist of perceived receptiveness, perceived cultivation, perceived customization, and perceived contact interactivity. Moreover, we introduce nationality as a control variable in this model, in order to explore the differences between three countries: America, China, and South Korea.

Triterpenoid saponins from Clinopodium chinense (Benth.) O. Kuntze and their biological activity

Yin-Di Zhu,Jing-Yi Hong,Feng-Da Bao,Na Xing,Ling-Tian Wang,Zhong-Hao Sun,Yun Luo,Hai Jiang,Xudong Xu,Nai-Liang Zhu,Hai-Feng Wu,Gui-Bo Sun,Jun-Shan Yang 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.12

Four new ursane-type triterpenoid saponins, clinopoursaponins A–D (1–4), six new oleanane-type triterpenoid saponins, clinopodiside VII–XII (5–10), as well as eight known triterpene analogues (11–18), were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. The structures of the new compounds were determined based on extensive spectral analyses, including 1D (1H and 13C) and 2D NMR experiments (COSY, NOESY, HSQC, 2D TOCSY, HSQC-TOCSY and HMBC), HR-ESI-MS and chemical methods. Compounds 1–18 were evaluated for their protective effects against anoxia/reoxygenation-induced apoptosis in H9c2 cells and cytotoxicities against murine mammary carcinoma cell line 4T1. Compounds 8, 9 and 18 exhibited significant protective effects, while compound 1 exhibited cytotoxic activity with IC50 value of 7.4 μm compared to 7.6 μm for the positive control 10-hydroxycamptothecin.

자동차 모노포스트 시트 하부 판넬에서 대한 강도해석

쑨디(Di Sun),박수진(Soojin Park),김진호(Jinho Kim) 한국자동차공학회 2022 한국자동차공학회 학술대회 및 전시회 Vol.2022 No.11

자동차 모노포스트 시트 레일의 형상 최적 설계

쑨디(Di Sun),박수진(Soojin Park),김진호(Jinho Kim) 한국자동차공학회 2023 한국자동차공학회 부문종합 학술대회 Vol.2023 No.5

A critical review of risks, characteristics, and treatment strategies for potentially toxic elements in wastewater from shale gas extraction

Sun, Yuqing,Wang, Di,Tsang, Daniel C.W.,Wang, Linling,Ok, Yong Sik,Feng, Yujie Pergamon 2019 Environment international Vol.125 No.-

<P><B>Abstract</B></P> <P>Shale gas extraction via horizontal drilling and hydraulic fracturing (HF) has enhanced gas production worldwide, which has altered global energy markets and reduced the prices of natural gas and oil. Water management has become the most challenging issue of HF, as it demands vast amounts of freshwater and generates high volumes of complex liquid wastes contaminated by diverse potentially toxic elements at variable rates. This critical review focuses on characterizing HF wastewater and establishing strategies to mitigate environmental impacts. High prioritization was given to the constituents with mean concentrations over 10 times greater than the maximum contamination level (MCL) guidelines for drinking water. A number of potentially harmful organic compounds in HF wastewaters were identified via the risk quotient approach to predict the associated toxicity for freshwater organisms in recipient surface waters. Currently, two options for HF wastewater treatment are preferred, i.e., disposal by deep well injection or on-site re-use as a fracturing fluid. Supplementary treatment will be enforced by increasingly rigorous regulations. Partial treatment and reuse remain the preferred method for managing HF wastewater where feasible. Otherwise, advanced technologies such as membrane separation/distillation, forward osmosis, mechanical vapor compression, electrocoagulation, advanced oxidation, and adsorption-biological treatment will be required to satisfy the sustainable requirements for reuse or surface discharge.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Shale oil and gas production via hydraulic fracturing poses risks to water quality and quantity. </LI> <LI> High risk level is related with the management of hydraulic fracturing wastewater. </LI> <LI> Wastewater quality data limitation is an obstacle for developing sustainable treatment strategies. </LI> <LI> Integration of multiple technologies is required for wastewater reuse or discharge. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Synthesis of BiVO₄@C Core-Shell Structure on Reduced Graphene Oxide with Enhanced Visible-Light Photocatalytic Activity

Sun, Zhihua,Li, Chenzhe,Zhu, Shenmin,Cho, Maenghyo,Chen, Zhixin,Cho, Kyeongjae,Liao, Yongliang,Yin, Chao,Zhang, Di Wiley (John WileySons) 2015 ChemSusChem Vol.8 No.16

Multi-Criteria decision making based on fuzzy measure

Sun, Yan,Feng, Di Convergence Society for SMB 2013 융합정보논문지 Vol.3 No.2

치명적인 사고를 막기 위해 드라이버 졸음 (DD)를 검출하는 다양한 최근 방법이 제안되고있다. 본 논문은 운전자의 눈에 폐쇄 속도를 모니터링 할 수 있는 기능을 AdaBoost 기반 물체 검출 알고리즘에 적용한 DD 탐지 시스템 구현에서 하드웨어/소프트웨어 공동 설계 방법을 제안한다. 소프트웨어 구성 요소는 DD 검출 알고리즘 중에서 필요한 기능성을 완전하게 달성하기 위해 전체적인 제어 및 논리 연산을 구현한다. 반면, 본 연구에서는 DD 검출 알고리즘의 중요한 기능은 처리를 가속화하기 위해 맞춤형 하드웨어 구성 요소를 통해 가속된다. 하드웨어/소프트웨어 아키텍처는 비디오 도터 보드와 알테라 DE2 보드에 구현되었습니다. 제안 된 구현의 성능을 평가하고 몇 가지 최근의 작품을 벤치마킹했다. Decision procedure was done with the evaluation of multi-criterion analysis. Importance of each criterion was considered through heuristically method, specially it was based on the heuristic least mean square algorithm. To consider coalition evaluation, it was carried out by calculation of Shapley index and Interaction value. The model output is also analyzed with the help of those two indexes, and the procedure was also displayed with details. Finally, the differences between the model output and the desired results are evaluated thoroughly, several problems are raised at the end of the example which require for further studying.

Different Association of Manganese Superoxide Dismutase Gene Polymorphisms with Risk of Prostate, Esophageal, and Lung Cancers: Evidence from a Meta-analysis of 20,025 Subjects

Sun, Guo-Gui,Wang, Ya-Di,Lu, Yi-Fang,Hu, Wan-Ning Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Altered expression or function of manganese superoxide dismutase (MnSOD) has been shown to be associated with cancer risk but assessment of gene polymorphisms has resulted in inconclusive data. Here a search of published data was made and 22 studies were recruited, covering 20,025 case and control subjects, for meta-analyses of the association of MnSOD polymorphisms with the risk of prostate, esophageal, and lung cancers. The data on 12 studies of prostate cancer (including 4,182 cases and 6,885 controls) showed a statistically significant association with the risk of development in co-dominant models and dominant models, but not in the recessive model. Subgroup analysis showed there was no statistically significant association of MnSOD polymorphisms with aggressive or nonaggressive prostate cancer in different genetic models. In addition, the data on four studies of esophageal cancer containing 620 cases and 909 controls showed a statistically significant association between MnSOD polymorphisms and risk in all comparison models. In contrast, the data on six studies of lung cancer with 3,375 cases and 4,050 controls showed that MnSOD polymorphisms were significantly associated with the decreased risk of lung cancer in the homozygote and dominant models, but not the heterozygote model. A subgroup analysis of the combination of MnSOD polymorphisms with tobacco smokers did not show any significant association with lung cancer risk, histological type, or clinical stage of lung cancer. The data from the current study indicated that the Ala allele MnSOD polymorphism is associated with increased risk of prostate and esophageal cancers, but with decreased risk of lung cancer. The underlying molecular mechanisms warrant further investigation.