中国企业创新内部软环境因素之限制及改善 - 兼与日韩企业比较

Luo Yun-pen 실천경영학회 2007 실천경영연구 Vol.2 No.1

It is important for enterprises to hold system viewpoint to carry on the enterprise innovation. This article summarizes various factors which influence enterprise innovation,and points out the significance of analyzing enterprise interior soft environment. After studying the experiences of enterprise innovation in Japan and SouthKorea, the article concludes that China enterprise innovation has been restricted by following factors :enterprise management idea, entrepreneur"s innovation spirit, enterprise innovation culture, business management system, and etc.. Finally, the article proposes relative countermeasures to improve it.

The Prevalence and Genotype of Human Papillomavirus from Patients with Genital Warts in Eastern Guangdong Province

Luo, Zhao-Yun,Chen, Qiang,Yang, Hui,Lin, Min,Chen, Chan-Yu,Yang, Chun,Yang, Li-Ye Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Low-risk human papillomavirus (LR-HPV) infection is the main cause of genital warts. LRHPV genotypes 6 and 11 are associated with genital warts, but there have only been a few published studies about the genotype-specific prevalence of HPV in genital warts in China. The objective of our study was to assess the prevalence of HPV genotypes for clinical cases involving both men and women and to evaluate the potential benefit of a quadrivalent (genotypes 6, 11, 16, and 18) HPV vaccine in eastern Guangdong province of China. Materials and Methods: A total of 696 eligible patients with genital warts were enrolled during the period Aug 2009 through Oct 2014. Specimens were collected from genital warts, the HPV GenoArray test was used for HPV detection and genotyping, which could detect 21 HPV genotypes, including genotypes 6, 11, 16, and 18. Results: Among the 696 cases, 675 samples were successfully genotyped. The median age of patients was 32.1 years (range, 16-67 years). The most prevalent genotypes were HPV-6 (285/675, 42.2%), HPV-11 (265/675, 39.3%), HPV-52 (52/675, 7.7%), HPV-16 (51/675, 7.56%), HPV-81 (50/675, 7.40%) and HPV-58 (37/675, 5.48%). Low-risk genotypes predominated, with a prevalence of 96.59%. The cumulative prevalence of genotypes 6 and 11 was 78.7% (531/675), the cumulative prevalence of genotypes 16 and 18 was 11.6% (78/675), and the cumulative prevalence of genotypes 6, 11, 16, and 18 was 82.5% (557/675). Conclusions: Our results provide strong evidence that, in eastern Guangdong, different from Western countries, the most prevalent low risk HPV genotypes in patients with genital warts are 6, 11 and 81. The quadrivalent HPV vaccine could prevent 82.5% of genital warts in eastern Guangdong.

Effect of notch position on creep damage for brazed joint

Luo, Yun,Jiang, Wenchun,Zhang, Qian,Zhang, Weiya,Woo, Wanchuck,Tu, Shan-Tung,Hao, Muming Elsevier Applied Science 2016 Advances in engineering software Vol.100 No.-

<P><B>Abstract</B></P> <P>In this paper, we investigated the effect of notch position on creep damage for Hastelloy C276-BNi2 brazed joint. Three different types of notches locate in edge of base metal (base notch), edge of filler metal (surface notch) and center of filler metal (inside notch) were compared, and the influence of notch geometric parameters on creep damage was also investigated. The results show that the different notch position and dimension generate different creep damage distributions and have a great influence on creep life. The creep failure is the easiest to occur in surface notch, then the base notch, and the last is inside notch. The brazed joint with higher maximum principal stress and von Mises stress generates creep failure easier. For the base notch, the failure time increases with the increase of base notch distance and the creep failure location moves gradually from the center of filler metal to notch tip. The notch locating away from filler metal is beneficial to reduce the creep damage in filler metal and enhance the creep life. For the inside notch, the failure time decreases with notch length increases and the maximum creep damage locates at notch tip. With the increase of inside notch width, the failure time increases first and then keep steadiness, and the failure location moves away from notch tip. The effects of notch position and dimension should be fully considered in creep failure analyses and life assessments of brazed joints.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The creep failure is easy generate in surface notch. </LI> <LI> The far away notch is helpful to reduce the creep damage of filler metal. </LI> <LI> As H increases, the failure location moves from filler metal to base metal. </LI> <LI> As H increases, the failure time increases first then keeps stable. </LI> <LI> The failure time decreases with L increases while it increases with W increases. </LI> </UL> </P>

Morphology and Molecular Characterization of Alternaria argyranthemi on Chrysanthemum coronarium in China

( Huan Luo ),( Zhen Zhou Xia ),( Yun Yun Chen ),( Yi Zhou ),( Jian Xin Deng ) 한국균학회 2018 Mycobiology Vol.46 No.3

Chrysanthemum coronarium is an economically important plant in Asia, and used medicinally, ornamentally and as a vegetable. In April 2017, leaf spot disease on C. coronarium was observed in Shiyan, Hubei, China. A single-spore isolate was obtained and identified based on morphology and sequence analysis using four regions (rDNA ITS, GAPDH, EF-1a, and RPB2). The results indicated that the fungus is Alternaria argyranthemi. The pathogenicity tests revealed that the species could cause severe leaf spot and blight disease on the host. This is the first report of leaf spot disease on C. coronarium caused by A. argyranthemi in the world, which is also a new record of Alternaria species in China.

Numerical simulations of interactions between solitary waves and elastic seawalls on rubble mound breakwaters

Yun-Feng Lou,Chuan Luo,Xian-Long Jin 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.3

Two dimensional numerical models and physical models have been developed to study the highly nonlinear interactions between waves and breakwaters, but several of these models consider the effects of the structural dynamic responses and the shape of the breakwater axis on the wave pressures. In this study, a multi-material Arbitrary Lagrangian Eulerian (ALE) method is developed to simulate the nonlinear interactions between nonlinear waves and elastic seawalls on a coastal rubble mound breakwater, and is validated experimentally. In the experiment, a solitary wave is generated and used with a physical breakwater model. The wave impact is validated computationally using a breakwater - flume coupling model that replicates the physical model. The computational results, including those for the wave pressure and the water-on-deck, are in good agreement with the experimental results. A local breakwater model is used to discuss the effects of the structural dynamic response and different design parameters of the breakwater on wave loads, together with pressure distribution up the seawall. A large-scale breakwater model is used to numerically study the large-scale wave impact problem and the horizontal distribution of the wave pressures on the seawalls.

Effect of Supplements Mn2+, Cu2+, and Aromatic Compounds and Penicillium decumbens on Lignocellulosic Enzyme Activity and Productivity of Catathelasma ventricosum

( Yun Tao Liu ),( Jun Sun ),( Zeyu Luo ),( Sheng Qi Rao ),( Yu Jie Su ),( Yan Jun Yang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

This is the first report on using Catathelasma ventricosum for production of fruiting body and lignocellulosic enzymes. To improve the laccase activity and productivity of mushroom, the substrate was added with different supplements (eight aromatic compounds, Mn2+, and Cu2+). Based on the results, all these supplements can improve the laccase activity and productivity of C. ventricosum, and it seems that there is a critical value of laccase activity that affects the productivity of C. ventricosum. In addition, when Penicillium decumbens was inoculated into the substrate that had been cultivated with C. ventricosum for 20 days, the highest values of laccase activity, FPA activity, and productivity of C. ventricosum were obtained. Moreover, the laccase activity showed a positive correlation with the productivity of C. ventricosum. Finally, the effect of Mn2+, Cu2+, and P. decumbens on laccase activity was investigated by response surface methodology (RSM).

Antihypertensive Effect of Few-flower Wild Rice (Zizania latifolia Turcz.) in Spontaneously Hypertensive Rats

Yun Deng,Yali Luo,Bingjun Qian,Zhenmin Liu,Yuanrong Zheng,Xiaoyong Song,Shaojuan Lai,Yanyun Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.2

The antihypertensive activity of few-flower wildrice was studied in spontaneously hypertensive rats (SHRs)with evaluation of blood pressure lowering effects andtranscriptional levels of the sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) gene that is regulated by AngiotensinII (Ang II). SHRs were randomly divided into 5 groupswith 6 rats each. The systolic blood pressure (SBP) reachedthe lowest point 3 h after administration of a single dose ofpaste made from few-flower wild rice stem powder. TheSBP of SHR in the relatively high amount of RSP (HRSP)administrated group was reduced by approximately 30mmHg, compared to the negative control group, and wasnot significantly different from the positive control IPPcontrol group at a dose of 1.5 mg/kg body weight (p>0.05). RSP administrated SHRs showed a significantly higherSERCA2a transcription level than negative control SHRs(p<0.05). RSP administration had no negative effects onglycometabolism of SHR.

Effects of puerarin on the Akt signaling pathway in bovine preadipocyte differentiation

Yun, Jinyan,Yu, Yongsheng,Zhou, Guoli,Luo, Xiaotong,Jin, Haiguo,Zhao, Yumin,Cao, Yang Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro. Methods: Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with oil red O. The content of triacylglycerol was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α, were measured using quantitative real-time polymerase chain reaction and western blotting, respectively. Results: The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPARγ (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPARγ expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway. Conclusion: Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.

ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

( Yun Deng ),( Bi Sheng Liu ),( Xiong Wei Fan ),( Yue Qun Wang ),( Ming Tang ),( Xiao Yang Mo ),( Yong Qing Li ),( Zao Chu Ying ),( Yong Qi Wan ),( Na Luo ),( Jun Mei Zhou ),( Xiu Shan Wu ),( Wu Zhou 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3): 193-198]

Circ-MAN1A2 Contributes to the Acquired Resistance of Gefitinib by Binding to miR-409-3p to Induce TWIST1 Expression in Non-small-cell Lung Cancer

Yun Li,Jinping Liu,Rong Luo,Yong You,Guiming Chen 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4

Gefitinib has been widely used as a firstgeneration epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor for non-small-cell lung cancer (NSCLC) patients with EGFR mutation. In this study, we explored the key molecules responsible for acquired Gefitinib resistance in NSCLC cells. 3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay was performed to analyze the Gefitinib resistance and proliferation ability of NSCLC cells. Cell proliferation was also assessed by colony formation assay. Flow cytometry was performed to analyze cell apoptosis. Wound healing assay and transwell invasion assay were performed to the migration and invasion abilities of NSCLC cells, respectively. The target relationship between microRNA-409-3p (miR-409-3p) and circular RNA mannosidase alpha class 1A member 2 (circ- MAN1A2) or twist family bHLH transcription factor 1 (TWIST1) was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. Circ-MAN1A2 level was markedly elevated in Gefitinib-resistant NSCLC cell lines and tissues. Circ-MAN1A2 interference sensitized Gefitinib-resistant NSCLC cells to Gefitinib. Furthermore, circ-MAN1A2 interference suppressed the proliferation, migration and invasion and promoted the apoptosis of Gefitinib-resistant NSCLC cells. Circ-MAN1A2 overexpression negatively regulated miR-409-3p level by directly binding to it. miR-409-3p silencing partly counteracted circ-MAN1A2 silencing-mediated anti-tumor effects in Gefitinib-resistant NSCLC cells. TWIST1 was a target of miR-409-3p, and miR-409-3p overexpression-induced antitumor effects in Gefitinib-resistant NSCLC cells were partly reversed by TWIST1 overexpression. Circ-MAN1A2 silencing aggravated Gefitinib-mediated inhibition of tumor growth in vivo. In conclusion, circ-MAN1A2 facilitated the acquired resistance of Gefitinib and other malignant behaviors of NSCLC cells through mediating miR-409-3p/ TWIST1 axis.