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정희선,박철 德成女子大學校 1993 德成女大論文集 Vol.22 No.-
Hi-sun Chung & Cheol Park. An Experimental Study of Corporate Advertising Effectiveness on Corporate Image Change. The purpose of this study is to examine the effectiveness of corporate advertising on corporate image through an experimental research design. The major findings of the study are as follows: (1) the corporate advertising has a positive effect on the corporate image change, but it is not strong enough to be of any statistical significance; (2) a significant difference in audience response is found with varying types of corporate advertising and also with varying nature of the pre-existing corporate image-the sympathetic advertisements are more successful than the persuasive ones in inducing the more positive response, and the groups with favorable pre-existing image showed a more positive response than those with hostile pre-existing image; and (3) the nature of pre-existing corporate image is more influential than the nature of corporate advertising in shaping new corporate image.
저칼슘식이로 사육한 생쥐의 골수세포 배양에 의한 파골세포양세포의 형성
박주철,강선주,임도선,김현만,고재승 대한구강해부학회 1995 대한구강해부학회지 Vol.19 No.1
Although it is now well established that osteoclasts are derived from mononuclear hematopoietic stem cells, most likely within the granulocyte-macrophage lineage, the exact differentiation process of osteoclast precusors has only been partially discovered. This study was carried out to investigate the following topics by examining the osteoclast-like cell formation from the calcium-deficient mouse bone marrow cells; 1) the effect of calcium deficiency on the formation of the mononuclear phagocytes and the mononuclear precusors of the osteoclast in the mouse bone marrow; 2) the effect of TNF (tumor necrosis factor)-α on the differentiation of osteoclast-like cells from the calcium-deficient mouse bone marrow culture; 3) the formation of the osteoclast-like cell from the calcium-deficient mouse bone marrow mononuclear cells and the relationship between the osteoblast cell line(MC3T3E1) and osteoclast-like cell formation; 4) formation of the osteoclast-like cells from the mononuclear cells which were col1ected from PTH-pretreated mouse bone marrow cells to investigate the relationship between PTH and calcium deficiency in osteoclast-like cell formation. Bone marrow cells were isolated from marrow of mouse feeding normal diet or calcium deficient diet for 7 and 14 days. Bone marrow mononuclear cells were fractionated by cetrifugation on Hypaque-Ficoli density gradients. PTH-pretreated mouse bone marrow mononuclear cells were obtained by collecting the nonadherent cells after the culture of normal mouse marrow cells with PTH for 6 days. The prepared cells were cultured on dentin disc at the concentration of 1.5-2 X 10^(6) cells per 0.5ml of a-MEM containing 10% FCS for 3, 5 and 7 days. Thereafter, TRAP staining, NSE staining, effect of calcitonin on the osteoclast-like cell and scanning electron microscope analysis of resorption lacunae on the dentin disc were performed. The results were as follows; 1. Number of the osteoclast precusors and mononuclear phagocytes in bone marrow was increased by calcium-deficient diet. 2. More osteoclast-like cells were formed from the calcium-deficient mouse bone marrow cells than from normal cells. 3. Formation of osteoclast-like cells was significantly stimulated by TNF-α in normal mouse bone marrow cells and calcium-deficient mouse bone marrow cells at concentration of l0ng/ml and 100ng/ml. 4. Osteoblast cell line(MC3T3E1) did not play an important role in osteoclast-like cell formation. 5. Formation of the osteoclast-like cells from the mononuclear cells which were collected from PTH-pretreated mouse bone marrow cells was similar to that of calcium-deficient mouse bone marrow mononuclear cells, which suggests that the effect of calcium deficiency in osteoclast formation may be mediated by PTH.
朴哲辰,曺哉銑 慶熙大學校 1989 論文集 Vol.18 No.-
In order to investigate the changes in sugar and organic acids of kimchi during fermentation, Chinese cabbage, radish and kimchi extracts were fermented by lactic acid bacteria and then analyzed acidity, pH, sugar and ( acid ic ac ) of fermented samples. The major organic acids in the samples were lactic, acetic, malic and succinic acid. Of these acids, lactic, acetic and citric acid were increased but malic and succinic acid were decreased during kimchi fermentation. The major sugars of the samples were fructose and glucose. The amount of sugars in these samples were decreased more rapidly when fermented at higher temperature than that of low temperature.
朴哲辰,曺哉銑 경희대학교 부설 식량자원개발연구소 1989 硏究論文集 Vol.10 No.-
In order to investigate the changes in sugar and organic acids of kimchi during fermentation, Chinese cabbage, radish and kimchi extracts were fermented by lactic acid bacteria and then analyzed acidity, pH, sugar and (acidic ac) of fermented samples. The major organic acids in the samples were lactic, acetic, malic and succinic acid Of these acids, lactic, acetic and citric acid were increased but malic and succinic acid were decreased during kimchi fermentation. The major sugars of the samples were fructose and glucose. The amount of sugars in these samples were decreased more rapidly when fermented at higher temperature than that of low temperature.
인간 타액선세포 및 선암종세포주의 분화 유도시 Transglutaminase발현과 고정의존성 성장과의 관계
박대열,이선경,이화정,장현주,민승기,김은철 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2
Transglutaminase(TGase) catalyzes the Ca^2+ dependent acyl transfer reaction to from γ-glutamyl lysine cross-links between substrate proteins. we have examined the expression of TGase 1 and TGase 2 enzyme activity and protein expression in differentiation induced salivary gland cells(HSG), and human salivary adenocarcinoma cell lines(SGT)in reation to anchorage independent growth. The TGase1 and TGase 2 activity in SGT was higher than in HSG, and there is no significant difference between TGase 2 and TGase1 cells. HSG cells showed no increase in TGase 1 and TGase 2 upon reaching post confluence and in 1.8mM Ca^2+. Expression of the TGase 1 and TGase 2 was induced by high cell density or by high calcium in SGT. No induction of TGase 1 & 2 protein was observed in postconfiuent HSG cells, but there was notable increases in TGase 1 & 2 protein in post confiuent SGT cells. This pattern of TGase 1 & 2 protein expression correlates with the pattern of particulate activity. SGT cells showed more colony size and colony forming activity in soft agar rather than HSG cells. The ability of SGT cells, containing high levels of TGase 2 and TGase1,to form colonies in soft agar suggests a role for this enzyme in anchorage independence.
박화진,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1
We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [^(3)H]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20 ㎍/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10 ㎍/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.
박준동,신동철,김순금,김부환 東亞大學校附設스포츠科學硏究所 2003 스포츠科學硏究論文集 Vol.21 No.-
The purpose of this study is to find ways of promoting and developing sports for the disabled in Korea. For the purpose, this researcher made a literature research about the matter, and proposed the following ways of promoting such sports. 1. Change in competent authorities of sports for the disabled 2. The supplementation, enactment and revision of laws applicable to sports for the disabled 3. Improvement in the social recognition of disabled persons and sports for the disabled and increased participation in such sports 4. The expansion and increased use of facilities of sports for the disabled and the development of supplementary convenience facilities 5. Increase in administrative and financial supports to bodies of sports for the disabled 6. The training and increased allotment of instructors of sports for the disabled 7. The development and active distribution of special sport programs 8. The promotion of sport events for the disabled and the training of professional disabled players 9. Strengthening of systems of the information of sports for the disabled 10. Promotion of special school sports