잡초성벼의 superoxide dismutase cDNA cloning과 재배벼로의 형질전환

박상규,박종석,이승인,서석철,김병극,조윤래,서학수 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

냉해나 한발등의 환경스트레스에 대해 저항성을 유발하는 유전자를 환경스트레스에 강한 잡초성벼로부터 선발하고 이들 유전자를 재배벼에 도입하여 도입유전자 산물의 과량 발현을 통해 냉해나 한발 등에 대한 저항성이 향상된 벼를 선발하고자 하였다. 잡초성벼인 Bhutan 14Ad로부터 냉해 및 한발 저항성 유전자로 알려진 superoxide dismutase (SOD) cDNA를 분리하고자 mRNA를 분리하고 이 분리된 mRNA를 이용해 reverse transcriptase PCR방법으로 SOD cDNA를 cloning 하였다. 그 결과 2종의 SOD cDNA가 cloning되어 SOD-A, SOD-B로 명명하였다. 이들 cDNA의 염기서열을 결정한 결과 이들은 아미노산 서열 상동성이 88.4%를 나타내었으며, SOD-A는 oryza sativa 계열의 Cu/Zn SOD유전자인 GenBank accession No. L36320와 99.3%로 동일하였으며, SOD-B는 accession No. D01000과 100% 동일하였다. 이들 SOD-A와 SOD-B cDNA를 재배벼인 낙동벼에 형질전환하여 형질전환체 벼를 선발하였으며, 이들 형질전환체 벼의 냉해저항성 및 한발저항성 검정을 통해 저항성이 향상된 형질전환체 벼를 선발하고 있다. Two different cDNA clones for superoxide dismutase (SOD) were isolated from an weedy rice variety (Oryza sativa, cv. Bhutanl4Ad) and were introduced into a cultivated rice variety (Oryza sativa, cv. Nakdong) in order to develop the environmental stress-resistant rice plants. Sequence analysis of the cloned cDNAs indicated that the deduced amino acid sequence of SOD-A is 88.4% identical to that of SOD-B. Furthermore, the nucleotide sequence of SOD-A is 99.3% identical to that of a Cu/Zn SOD gene of Oryza sativa (GenBank accession No. L36320). The nucleotide sequence of SOD-B was identical to that of the previously published SOD gene (Accession No. D01000). A cultivated rice variety, Nakdong-byeo, was transformed with chimenc SOD genes containing a actin promoter of rice and pin2 terminator using a particle bombardment technique. Transformed calli were selected on an selection medium containing phosphinothricin (PPT). Transgenic rice plants were regenerated from the PPT-resistant calli. PCR analysis with genomic DNAs from transgenic plants revealed that transgenes are introduced into rice genome.

2축 편심축력을 받는 고강도 콘크리트 기둥의 등가응력블럭 수정

박준용,유석형,반병열,신성우 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The characteristics of stress distribution of non rectangular compressed area in the RC columns are different to those of rectangular compressed area. Nevertheless, in ACI 318-99 code, the design tables for columns subjected to axial loads with bidirectional eccentricities are based on the parameters recommended for rectangular stress block(RSB) of rectangular compressed areas. In this study the characteristics of stress distribution through both angle and depth of neutral axis are observed and formulated rationally. And the modified parameters of rectangular stress block(MRSB) for non rectangular compressed area is proposed. And the computer program using MRSB for the biaxial bending analysis of RC columns in developed and the results of MRSB are compared to RSB and experimental results respectively.

탈회상아질기질이 임프란트의 골유착에 미치는 영향

박석인,류선열 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

The purpose of this study was to investigate the effect of demineralized dentin matrix on osseointegration of implants. Seven mongrel dogs of either sex, weighing about 15kg, were used. The lower 1st, 2nd, 3rd, and 4th premolars of both sides were extracted under general anesthesia. Implants were inserted 3 months after extraction of the teeth. In the control group, socket wall around the implant was sutured without any graft material. The experimental groups were divided into the two : the DDM group was filled with demineralized dentin matrix into the hole of the implant and the DDCM group filled with demineralized dentin and collagen matrix. The animals were killed 1, 2, 4, 8, and 12th weeks after the implantation. Tissue specimens obtained were processed for light and fluorescent microscopic examination. Results obtained were as follows : 1. At one week after the implantation fibrous connective tissue from the surrounding periosteum was observed at the superior portion of the alveolar bone-implant interface and blood clot was formed within the hole of the implant in control group. Endosteal proliferation from the cut surface of the compact bone was observed in the both experimental groups. 2. At two weeks after the implantation the interspace between the alveolar bone and implant became narrow, and endosteal proliferation and woven bone formation from the cut compact bone were noted in control group. Newly formed trabecular bone was blended with demineralized dentin matrix in the both experimental groups. 3. At four weeks after the implantation some fibrous tissues were still observed in the interspace between the alveolar bone and implant in control group. However, with osteoinduction and osteoconduction by deminealized dentin matrix, osseointegration without intervening of fibrous tissue was observed in the both experimental groups. 4. At eight and twelve weeks after the implantation a complete osseointegration was noted and demineralized dentin matrix was almost resorbed in all groups. 5. By fluorescent microscopic examination, bone formation and osseointegration were noted earlier in the experimental groups than in the control group. 6. Bone healing of the DDCM group was similar to that of the DDM group. These results suggest that demineralized dentin matrix can be used as a useful material for early fixation of implants by promoting new bone formation and osseointegration of implants.

Crystal structure of single-domain VL of an anti-DNA binding antibody 3D8 scFv and its active site revealed by complex structures of a small molecule and metals

Park, Suk-Youl,Lee, Woo-Ram,Lee, Suk-Chan,Kwon, Myung-Hee,Kim, Yong-Sung,Kim, Jeong-Sun Wiley Subscription Services, Inc., A Wiley Company 2008 Proteins Vol.71 No.4

Phase determination of a homogentisate dioxygenase from Comamonas sp. strain P19

Suk-Youl Park,Byoung Yul Soh,Jong-Chan Chae,Jeong-Sun Kim 한국구조생물학회 2017 Biodesign Vol.5 No.3

In Comamonas sp. strain P19, homogentisate 1,2-dioxygenase (HGO) catalyzes the conversion of homogentisate to 4-maleylacetoacetate by aromatic ring scission, in the breakdown of tyrosine and phenylalanine. To determine the molecular background of the enzymatic mechanism of HGO in this zinc-resistant organism, hmgA encoding HGO of Comamonas sp. strain P19 was cloned, and the expressed protein was purified. The protein was crystallized in solutions I [25% (w/v) polyethylene glycol 3350 and 0.1 M trisodium citrate at pH 5.6] and II [1.4 M ammonium tartrate and 0.1 M bisTris at pH 5.5]. X-ray diffraction data were collected to 1.8 Å resolution using synchrotron radiation. The crystal belongs to the orthorhombic space group P2 1 2 1 2 1 , with unit cell dimensions of a = 73.2 Å, b = 100.0 Å, and c = 134.9 Å. A traceable electron density map was calculated using anomalous diffraction data obtained from a crystal soaked in zinc ions.

The Protein Crystallography Beamlines at the Pohang Light Source II

Suk-Youl Park,Sung-Chul Ha,Yeon-Gil Kim 한국구조생물학회 2017 Biodesign Vol.5 No.1

Pohang Light Source II (PLS-II) is a 3.0 GeV national synchrotron radiation facility in Korea. Three protein crystallography beamlines at PLS-II are available for national and international users in the structural biology community. All three beamlines (BL-5C, BL-7A and BL-11C) are illuminated with an in-vacuum undulator source, which provides a tunable high brilliant X-ray. BL-5C and BL-7A are designed for routine protein crystallography with a default beam size of 100 µm, while BL-11C is designed for micro-crystallography with a default beam size of 10 μm. This report summarizes the primary parameters of the three beamlines, and describes user productivity and recent experimental results.

Crystal structure and its proposed nucleolytic site of the single domain catalytic antibody 3D8-VH

Suk-Youl Park,Bo-Na Kim,Dong-Ki Choi,Pil-Won Seo,Do-Heon Gu,Yong-Sung Kim,Jeong-Sun Kim 한국구조생물학회 2016 Biodesign Vol.4 No.1

Catalytic antibodies are monoclonal antibodies with catalytic activities. An anti-DNA antibody can bind DNA and hydrolyze it with modest catalytic activity. The monoclonal antibody 3D8 from the autoimmune-disease prone MRL-lpr/lpr mouse has been used to make a recombinant single-chain variable-fragment antibody (3D8 scFv) that hydrolyzes DNA. The catalytic site is composed of several hyper-variable loops. Two His residues from the variable domains of the heavy chain (VH) and the light chain (VL) are responsible for DNA hydrolysis. Interestingly, two single domain antibodies derived from 3D8 scFv, called 3D8-VH and 3D8-VL, also bind and hydrolyze DNA although their activities are significantly lower than those of 3D8 scFv. Here, we report the crystal structure of 3D8-VH, which revealed no significant difference compared to the VH domain from 3D8 scFv. The 3D8-VH antibody is also structurally very similar to 3D8-VL that has a catalytic metal ion on the newly exposed surface-curvature. The observed structural features of 3D8-VH, along with the fact that it retained catalytic activity when the His residue that was catalytic in the 3D8 scFv was replaced, suggest that 3D8-VH also has its DNA binding and cleavage activity on the newly exposed surface. These findings provide new insights into substrate recognition and catalysis by single domain anti-DNA antibodies.

Electrical Properties of Alcohol Vapor Sensors Based on Porous Silicon

Park, Kwang-Youl,Kang, Kyung-Suk,Kim, Seong-Jeen,Lee, Sang-Hoon,Park, Bok-Gil,Sung, Man-Young The Korean Institute of Electrical and Electronic 2003 전기전자재료학회논문지 Vol.16 No.12

In this work, we fabricated a gas-sensing device based on porous silicon(PS), and its C-V properties were investigated for sensing alcohol vapor. The structure of the sensor consists of thin Au/oxidized PS/PS/P-Si/Al, where the p-Si is etched anisotropically to be prepared into a membrane-shape. We used alcohol gases vaporized from different alcohol (or ethanol) solutions mixed with pure water at 36$^{\circ}C$, similarly with an alcohol breath measurement to check drunk driving. As the result, I-V curves showed typical tunneling property, and C-V curves were shaped like those of a MIS (metal-insulator-semiconductor) capacitor, where the capacitance in accumulation was increased with alcohol vapor concentration.

Structural basis of the specific interaction of SMRT corepressor with histone deacetylase 4

Park, Suk-Youl,Kim, Gwang Sik,Hwang, Hyo-Jeong,Nam, Taek-Hyun,Park, Hee-Sae,Song, Jaeyoung,Jang, Tae-Ho,Lee, Young Chul,Kim, Jeong-Sun Oxford University Press 2018 Nucleic acids research Vol.46 No.22

<P><B>Abstract</B></P><P>Modification of chromatin and related transcription factors by histone deacetylases (HDACs) is one of the major strategies for controlling gene expression in eukaryotes. The HDAC domains of class IIa HDACs repress the respective target genes by interacting with the C-terminal region of the silencing mediator for retinoid and thyroid receptor (SMRT) repression domain 3 (SRD3c). However, latent catalytic activity suggests that their roles as deacetylases in gene regulation are unclear. Here, we found that two conserved GSI-containing motifs of SRD3c are critical for HDAC4 binding. Two SMRT peptides including these motifs commonly form a β-hairpin structure in the cleft and block the catalytic entry site of HDAC4. They interact mainly with class IIa HDAC-specific residues of HDAC4 in a closed conformation. Structure-guided mutagenesis confirmed critical interactions between the SMRT peptides and HDAC4 and –5 as well as the contribution of the Arg1369 residue in the first motif for optimal binding to the two HDACs. These results indicate that SMRT binding does not activate the cryptic deacetylase activity of HDAC4 and explain how class IIa HDACs and the SMRT-HDAC3 complex are coordinated during gene regulation.</P>

Effects of shoes-heel height on static balance and isokinetic torques of ankle joint in collegiate women

Jae-Wan Park,Denny Eun,Young-Yun Jin,Yong-Seok Jee,Hye-Yoon Park,Min-Su Kim,Gwang-Suk Hyun,Jun-Youl Cha,Jung-Min Park,Nam-Heung Cho,Il-Gyu Ko,Jun-Jang Jin,Sung-Eun Kim 한국운동재활학회 2015 한국운동재활학회 학술대회 Vol.2015 No.10

The number of collegiate females wearing heel shoes is ever increasing. Although high-heeled shoes offer shapely figures, they have been associated with instability and falling, leading to ankle injuries etc. Furthermore, high-heels negatively affect the normal lining of a female curvature, aggravating chronic lumbar diseases. Despite its significant importance, the number of researches on high-heels is limited and the effects of shoes-heel height haven’t been previously reported. Thus, the purpose of this study was to investigate the balance ability and ankle muscular function of female college students. In the present study, the effects of wearing of three types of shoes-heel height on static balance and isokinetic ankle muscular function were investigated. The 78 of habitual wearers of three different types shoes for 40.10 ± 10.12 weeks were recruited in this study (mean age: 22.60 ± 0.65 years). The participants were grouped by the shoe-heel height; low-heels group (LH, n=18), mid-heels group (MH, n=34), and high-heels group (HH, n=26). The heel height of three groups were 3.77 ± 1.69 cm (LH), 7.43 ± 0.53 cm (MH) and 9.50 ± 0.64 cm (HH), respectively. All participants were tested for their body composition and static balance ability. Also, their ankle joints were isokinetically tested for plantarflexion / dorsiflexion and eversion / inversion at 60d/s and 120d/s, respectively. Statistical analysis was conducted with the SPSS software (ver 21.0; SPSS Inc, Chicago, IL). All data are were reported as mean ± standard deviation. Prior to the comparison of measurements including body composition, static balance, and isokinetic torque variables, the descriptive statistics were calculated for all dependent variables. The among-group factor was the study groups (i.e. LH vs MH vs HH). Because the data for this study were not normally distributed, we analyzed the data using non-parametric tests. The Kruskal-Wallis rank test was conducted to identify differences among groups. The significance level for all analyses was set a priori at p ≤0.05. Our results of this present study may summarize as follow. First, compared to LH and MH groups, the dorsiflexor peak torque of HH was significantly higher in dominant and non-dominant ankles. Although the dorsiflexor work per repetition and total work of HH were higher than other two groups, only the variables of non-dominant ankle was significantly higher. The dorsiflexor fatigue index of HH showed the lower tendency compared to LH and MH groups. Second, compared to LH and MH groups, the plantarflexor peak torque of HH was significantly higher in dominant and non-dominant ankles. The plantarflexor work per repetition and total work of HH also were significantly higher than other two groups in both ankles. The plantarflexor fatigue indices of HH showed the lower tendency than those of both groups. Third, compared to LH and MH groups, the evertor peak torque of HH was significantly higher in dominant and non-dominant ankles. The evertor work per repetition and total work of HH showed the higher tendency than other two groups. Although the evertor fatigue index in dominant side of HH was significantly lower than those of both groups, that of non-dominant side showed the lower tendency compared to LH and MH. Last, compared to LH and MH, the invertor peak torque of HH represented the lower tendency in dominant and non-dominant ankles. The invertor work per repetition and total work of HH were significantly lower than those of other two groups except for work per repetition of non-dominant side. The invertor fatigue indices in both sides of HH showed the lower tendency compared to LH and MH. In conclusion, the results showed that the wearing of high-heels for short years had significantly increased isokinetic muscular functions (dorsiflexor, plantarflexor, evertor) of the ankles except for invertor compared to wearing of low or mid heels. We may confirm that the wearing of high-heels help to enhance the strength and endurance of the ankle joints and these results can affect to increase the static balance in female students under about 1 year.