http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Seo, Nu-Ri,Ji, Min-Kyung,Park, Sang-Won,Lee, Kwangmin,Bae, Jung-Chan,Yun, Kwi-Dug,Park, Chan,Oh, Gye-Jeong,Kim, Ji-Hyun,Lim, Hyun-Pil American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.4
<P>Porphyromonas gingivalis (P. gingivalis) is one of main bacteria that adheres to the surface of dental implants and causes peri-implantitis. The purpose of this study was to observe the surface characteristics of titanium processed with either titanium nitride (TiN) sputter coating or plasma nitriding and to evaluate the subsequent adhesion of P. gingivalis. Specimens were divided into three groups: commercially pure (CP) titanium (control group), TiN sputter-coated titanium (group S), and plasma-nitrided titanium (group P). Surface characteristics such as roughness, morphology, and the formation of a thin TiN film or a nitriding layer were assessed. Adhesion of P. gingivalis in the three groups was determined by means of the crystal violet staining assay, and results were compared with one-way ANOVA, with post hoc comparison using Tukey's test (alpha = 0.05). Surface roughness values for the control group, group S, and group P were 0.08 +/- 0.02 mu m, 0.19 +/- 0.04 mu m, and 0.13 +/- 0.02 mu m, respectively. In group S, the TiN layer was 1.36 +/- 0.1 mu m thick, and nitrogen was detected on the surface of the specimens in group P, confirming formation of a nitrided layer. The level of adhesion in group P was significantly higher than that in the control group and in group S (p < 0.05), but there was no significant difference between the control group and group S. Within the limitations of this study, TiN sputter coating did not affect adhesion of P. gingivalis on the titanium surface, whereas adhesion was increased on the plasma-nitrided titanium surface.</P>
( Pil-won Seo ),( Ho-chang Ryu ),( Do-heon Gu ),( Hee-sae Park ),( Suk-youl Park ),( Jeong-sun Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.8
2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, which catalyzes aldol cleavage and condensation reactions, has two distinct substrate-binding sites. The substrate-binding mode at the catalytic site and Schiff-base formation have been well studied. However, structural information on the phosphate-binding loop (P-loop) is limited. Zymomonas mobilis KDPG aldolase is one of the aldolases with a wide substrate spectrum. Its structure in complex with the substrate-mimicking 3-phosphoglycerate (3PG) shows that the phosphate moiety of 3PG interacts with the P-loop and a nearby conserved serine residue. 3PG-binding to the P-loop replaces water molecules aligned from the P-loop to the catalytic site, as observed in the apostructure. The extra electron density near the P-loop and comparison with other aldolases suggest the diversity and flexibility of the serine-containing loop among KDPG aldolases. These structural data may help to understand the substrate-binding mode and the broad substrate specificity of the Zymomonas KDPG aldolase.
Pil-Won Seo,Jeong-Sun Kim 한국구조생물학회 2018 Biodesign Vol.6 No.3
Bacterial peptidoglycan is necessary for bacterial survival against environmental osmotic pressure and is a relevant target for development of anti-bacterial drugs. Formation of a covalent bond between a carbohydrate and an amino acid is a key chemical process for peptidoglycan bio-synthesis. UDP-N-acetylmuramic acid (UDPMurNAc):L-alanine ligase (MurC) is an ATP-dependent amide bond ligase to form an UDP-MurNAc-L-alanine required in bacterial peptidoglycan. To provide a structural background for development of tuberculosis-specific antibiotics, Mycobacterium bovis MurC (MbMurC), which has sequence identity of 100% to M. tuberculosis, was cloned and expressed. The purified protein was crystallized from the precipitant of 0.1 M HEPES (pH 7.0), 0.2 M NaCl, 24% (w/v) polyethylene glycol 1.5K, and 10% (v/v) 2-methyl-2,4-pentanediol. Diffraction data were collected to 2.3 Å resolution. The crystal belonged to the primitive monoclinic space P2 1 with unit-cell parameters a = 65.30 Å, b = 76.70 Å, c =103.96 Å, α = γ = 90°, and β = 106.0°. The spatial positions of the two protein molecules in the asymmetric unit were determined by molecular replacement using the sequentially related Yersinia pestis MurC structure.
Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media
Pil-Won Seo,김정선 한국구조생물학회 2022 Biodesign Vol.10 No.4
Fructose-6-phosphate aldolase 1 (FSA) from Aeromonas media (AmFSA) is a class I aldolase characterized by the formation of a Schiff-base intermediate between a conserved lysine residue and a carbonyl substrate. FSA has shown high stereoselectivity and broad substrate spectrum. To provide a structural background for engineering as a catalyst for desired reaction product, AmFSA was cloned and expressed. The purified protein was crystallized from the precipitant of 0.1 M sodium acetate (pH 5.0), 1.4 M ammonium sulfate, and 10 mM β-mercaptoethanol. Diffraction data were collected to 2.1 Å resolution. The crystal belonged to the orthorhombic space group C2221 with unit-cell parameters a = 93.80 Å, b = 175.27 Å, c = 150.99 Å, and α = β = γ = 90°. The spatial positions of the five protein molecules in the asymmetric unit were determined by molecular replacement using the sequentially related Escherichia coli FSA structure.
Seo, Dongwook,Lee, Si Un,Oh, Chang Wan,Kwon, O-Ki,Ban, Seung Pil,Kim, Tackeun,Byoun, Hyoung Soo,Kim, Young Deok,Lee, Yongjae,Won, Yu Deok,Bang, Jae Seung The Korean Neurosurgical Society 2019 Journal of Korean neurosurgical society Vol.62 No.6
Objective : To analyze the angiographic features and clinical course, including treatment outcomes and the natural course, of fusiform middle cerebral artery aneurysms (FMCAAs) according to their location, size, and configuration. Methods : We reviewed the literature on adult cases of FMCAAs published from 1980 to 2018; from 25 papers, 112 FMCAA cases, for which the location, size, and configuration could be identified, were included in this study. Additionally, 33 FMCAA cases in our hospital were included, from which 16 were assigned to the observation group. Thus, a total of 145 adult FMCAA cases were included. We classified the FMCAAs according to their location (l-type 1, beginning from prebifurcation; l-type 2, beginning from bifurcation; l-type 3, beginning from postbifurcation), size (small, <10 mm; large, ${\geq}10mm$; giant, ${\geq}25mm$), and configuration (c-type 1, classic dissecting aneurysm; c-type 2, segmental ectasia; c-type 3, dolichoectatic dissecting aneurysm). Results : The c-type 3 was more commonly diagnosed with ischemic symptoms (31.8%) than hemorrhage (13.6%), while 40.9% were found accidentally. In contrast, c-type 2 was more commonly diagnosed with hemorrhagic symptoms (14.9%) than ischemic symptoms (10.6%), and 72.3% were accidentally discovered. According to location, ischemic symptoms and hemorrhage were the most frequent symptoms in l-type 1 (28.6%) and l-type 3 (34.6%), respectively. Most of l-type 2 FMCAAs were found incidentally (68.4%). Based on the size of FMCAAs, only 11.1% of small aneurysms were found to be hemorrhagic, while 18.9% and 26.0% of large and giant aneurysms were hemorrhagic, respectively. Although four aneurysms of the 16 FMCAAs in the observation group increased in size and one aneurysm decreased in size during the observation period, no rupture was seen in any case and there were no significant predictors of aneurysm enlargement. Of 104 FMCAAs treated, 14 cases (13.5%) were aggravated than before surgery and all the aggravated cases were l-type 1. Conclusion : While ischemic symptoms occurred more frequently in l-type 1 and c-type 3, hemorrhagic rather than ischemic symptoms occurred more frequently in l-type 3 and c-type 2. In case of l-type 1 FMCAAs, more caution is required in determining the treatment due to the relatively high complication rate.
Pil Moon Kang,Won Ik Seo,Jae Il Chung 대한비뇨기종양학회 2014 대한비뇨기종양학회지 Vol.12 No.2
Purpose: We report our experience with modified 6+3 maintenance BCG therapy compared with a 6-week induction course over the past 10 years and its effects on recurrence and progression. Materials and Methods: Between January 2001 and June 2012, a total of 181 patients treated with NMIBC underwent TUR and intravesical BCG. BCG was administered over a 6-week course in 88 patients, and was administered for six weeks followed by three weekly instillations at 3, 6, 9, 12, 18, 24, and 36 months (modified 6+3 regimen) in 93 patients. The recurrence rate, time to recurrence, and progression rate were assessed and analyzed. Results: A total of 30 of 181 patients were ineligible. Seventy-three patients in the 6-week induction group and seventy-eight patients in maintenance group were eligible. Median follow-up was 37.4 months in the maintenance group and 32.9 months in the 6-week course group. The estimated overall recurrence rate and median recurrence-free survival were 21.8% (17/78) and 23.4 months (95% confidence interval [CI] 7-84) in the maintenance group and 35.6% (26/73) and 21.5 months (6-80) in the 6-week course group (log rank p=0.002), respectively. Estimated median times for progression-free survival were 44.0 months (95% CI 7-84) in the maintenance group and 25.7 months (14-41) in the 6-week course group (log rank p=0.0815). The overall rates of adverse effects were 27.9% (26/93) in the maintenance group and 25.0% (22/88) in the 6-week course group. Conclusions: Compared to the standard 6-week course, maintenance BCG immunotherapy was beneficial for preventing recurrence in patients with non-muscle-invasive bladder cancer.
Superplastic Deformation of Defect-Free Au Nanowires via Coherent Twin Propagation
Seo, Jong-Hyun,Yoo, Youngdong,Park, Na-Young,Yoon, Sang-Won,Lee, Hyoban,Han, Sol,Lee, Seok-Woo,Seong, Tae-Yeon,Lee, Seung-Cheol,Lee, Kon-Bae,Cha, Pil-Ryung,Park, Harold S.,Kim, Bongsoo,Ahn, Jae-Pyoung American Chemical Society 2011 NANO LETTERS Vol.11 No.8
<P>We report that defect-free Au nanowires show superplasticity on tensile deformation. Evidences from high-resolution electron microscopes indicated that the plastic deformation proceeds layer-by-layer in an atomically coherent fashion to a long distance. Furthermore, the stress–strain curve provides full interpretation of the deformation. After initial superelastic deformation, the nanowire shows superplastic deformation induced by coherent twin propagation, completely reorientating the crystal from <110> to <100>. Uniquely well-disciplined and long-propagating atomic movements deduced here are ascribed to the superb crystallinity as well as the radial confinement of the Au nanowires.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2011/nalefd.2011.11.issue-8/nl2022306/production/images/medium/nl-2011-022306_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl2022306'>ACS Electronic Supporting Info</A></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl2022306'>ACS Electronic Supporting Info</A></P>