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내독소로 유도되는 Nuclear Factor Kappa B 활성화에 미치는 Src Family Kinase의 조절기전
김희재,이혜원,이희수,하종식,이지희 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2
목적: Src family tyrosine kinases (TK)가 내독소로 인해 유도되는 NF-kB 활성화 신호전달체계에 연관되어 있음이 보고된 바 있다. 또한 내독소(LPS)나 TNF a와 같은 여러 자극제는 IkB-a 의 serine기 또는 tyrosine기의 인산화를 통하여 NF-kB를 활성화 시킨다고 알려진 바 있다. 이에 본 연구에서는 RAW 264.7 대식세포에 내독소 투여 시 유도되는 NF-kB활성화 및 NF-kB 의존성 염증성인자 생성에 대한 Src TK의 역할에 대해 규명하고자 한다. 방법: American Type Culture Collection 에서 구입한 생쥐의 대식세포, RAW264.를 내독소(LPS)에 노출시킨 후 damnacanthal나 PP1을 처리하여, EMSA, Nitrite assay, Western blot을 통하여Src TK가 NF-kB활성과 염증인자의 생성에 있어서 어떤 역할을 하는지에 대하여 조사하였고 내독소 투여로 인한 NF-kB활성에 있어서 Src TK의 기본적인 작용기전에 대해 조사하였다. 결과: Damnacanthal이나 PP1은 Src TK 특이적 억제제로 알려져 있는데, 본 연구에서는 Src TK의 특이적 억제제인 damnacanthal 이나 PP1을 사용하였고, RAW 264.7 대식세포에서 Src TK 특이 억제제의 전처치는 내독소로 유도되는 NF-kB활성을 차단시켰다. 또한 내독소 투여로 증가된 NO 생성은 damnacanthal이나 PP1에 의하여 억제되었다. 이런 TK kinase 억제제는 내독소로 유도되는 serine 기 인산화와 IkB-a 분해를 억제시켰다. 결론: Src kinase 특이적 억제제인 damnacanthal 그리고 PP1이 RAW 264.7세포에서 내독소로 유도되는 NF-kB활성과 Nitric Oxide 생성을 차단시켰다. 또한. Damna-canthal이나 PP1은 내독소로 유도된 serine기 인산화와 IkB-a의 분해를 억제시켰다. Objectives : Src family tyrosine kinases(TK) have been found to be involved in LPS induc-tion of signal cascades. Furthermore Lipopolysaccharide(LPS) or Tumor necrosis factor alpha (TNF-a) activate nuclear transcription factor kB(NF-kB) by inducing serine or tyrosine pho-sphorylation of the inhibitory subunit of NF-k B(I k B- a). In this study, it is our purpose to search the role of Src TK in LPS induced activation of NF-k B and NF-k B dependent induced inflam-matory factors. Methods : Nuclear extracts were prepared from RAW 264.7 cells pretreated with damnacan-thal or PP1 and then stimulate with LPS. After that, we figured out the dffects of inhibition of Src family kinases on LPS-induced activation of NF-kB by EMSA. We investigated effects of damnacanthal of PP1 on the production of NO by Griess assay and LPS-induced serine phos-phorylation and degradation of Ik B-a by Western blots in LPS-stimulated RAW263.7 cells. Results : Inhibition of Src TK with damnacanthal or PP1 blocked LPS-induced NF-kB acti-vation at the range of nanomolar concentrations. Substantial inhibition in LPS-induced production of NO was also observed in cells treated with damnacanthal or PP1. These kinase inhibitors blocked LPS-induced the serine phosphorylation, and the degradation of Ik B-a. Conclusion : we investigated the role of Src TK in NF-k B activation and production of nitric oxide (NO) in LPS stimulated RAW 264.7 macrophages and the underlying mechanism by which Src TK play a role in LPS-induction of the possible pathways leading to NF-k B activation. Src kinase specific inhibitors, damnacanthal and PP1 blocked LPS induced activating NF-k B and producing Nitric Oxide in Raw 264.7 machrophages. Moreover, Damnacanthal and PP1 inhibited LPS induced serine phosphorylation and degradation of Ik B-a.
식이 단백질의 급원에 따른 당뇨쥐의 혈당 및 혈청지질에 미치는 영향
이수진,김영진,박수정,김희정,이윤희,유영상 동아시아식생활학회 1997 동아시아식생활학회지 Vol.7 No.2
This study was designed to find out the effects of soybean protein and milk protein between nondiabetic and diabetic rats. The experimental results are summarized as follows. 1. Total food intake was higher in diabetic soybean protein group than other groups but it was not significant. 2. The change of body weight was lower in diabetic soybean protein group than other groups and the soybean protein was effective to maintain the ideal body weight. 3. The effects of lowering total cholesterol and glucose in serum was higher in soybean protein groups than the milk protein groups.
A Near-Infrared Probe Tracks and Treats Lung Tumor Initiating Cells by Targeting HMOX2
Kim, Jong-Jin,Lee, Yong-An,Su, Dongdong,Lee, Jungyeol,Park, Sung-Jin,Kim, Beomsue,Jane Lee, Jia Hui,Liu, Xiao,Kim, Seong Soon,Bae, Myung Ae,Lee, Jun-Seok,Hong, Seong Cheol,Wang, Lu,Samanta, Animesh,Kw American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.37
<P>Tumor initiating cells (TIC) are resistant to conventional anticancer therapy and associated with metastasis and relapse in cancer. Although various TIC markers and their antibodies have been proposed, it is limited to the use of antibodies for in vivo imaging or treatment of TIC. In this study, we discovered heme oxygenase 2 (HMOX2) as a novel biomarker for TIC and developed a selective small molecule probe TiNIR (tumor initiating cell probe with near infrared). TiNIR detects and enriches the functionally active TIC in human lung tumors, and through the photoacoustic property, TiNIR also visualizes lung TIC in the patient-derived xenograft (PDX) model. Furthermore, we demonstrate that TiNIR inhibits tumor growth by blocking the function of HMOX2, resulting in significantly increased survival rates of the cancer model mice. The novel therapeutic target HMOX2 and its fluorescent ligand TiNIR will open a new path for the molecular level of lung TIC diagnosis and treatment.</P> [FIG OMISSION]</BR>
Kim, Duck-Hyun,Kim, Hui-Su,Hwang, Dae-Sik,Kim, Hee-Jin,Hagiwara, Atsushi,Lee, Jae-Seong,Jeong, Chang-Bum Academic Press 2017 General and comparative endocrinology Vol.252 No.-
<P><B>Abstract</B></P> <P>Nuclear receptors (NRs) are a large family of transcription factors that are involved in many fundamental biological processes. NRs are considered to have originated from a common ancestor, and are highly conserved throughout the whole animal taxa. Therefore, the genome-wide identification of <I>NR</I> genes in an animal taxon can provide insight into the evolutionary tendencies of NRs. Here, we identified all the <I>NR</I> genes in the monogonont rotifer <I>Brachionus</I> spp., which are considered an ecologically key species due to their abundance and world-wide distribution. The NR family was composed of 40, 32, 29, and 32 genes in the genomes of the rotifers <I>B</I>. <I>calyciflorus</I>, <I>B. koreanus</I>, <I>B. plicatilis</I>, and <I>B. rotundiformis</I>, respectively, which were classified into seven distinct subfamilies. The composition of each subfamily was highly conserved between species, except for <I>NR1O</I> genes, suggesting that they have undergone sporadic evolutionary processes for adaptation to their different environmental pressures. In addition, despite the dynamics of NR evolution, the significance of the conserved endocrine system, particularly for estrogen receptor (ER)-signaling, in rotifers was discussed on the basis of phylogenetic analyses. The results of this study may help provide a better understanding the evolution of NRs, and expand our knowledge of rotifer endocrine systems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fourty, 32, 29, and 32 <I>NR</I> genes were identified in the four rotifer <I>Brachionus</I> spp. </LI> <LI> Species-specific evolution of <I>NR1O</I> genes was identified. </LI> <LI> The evolutionary scenario of ER-signaling pathways in <I>Brachionus</I> spp. have speculated on the basis of our phylogenetic analysis. </LI> </UL> </P>
Su-Mi Kim,Seung Hee Ryu,Hui-Don Choi,Sung-Su Kim,Jeong-Hwan Kim,Jong-Sang Kim 한국식품영양과학회 1998 Preventive Nutrition and Food Science Vol.3 No.3
There is extensive evidence suggesting the protective role of fruits and vegetables against chemically induced carcinogenesis. We have tested the ability of a representative range of Korean vegetables to act as blocking agents against neoplastic initiation by determining the induction level of quinone reductase, an anticarcinogenic marker enzyme, in hepa1c1c7 cells exposed to vegetable extracts. Among thirty vegetables tested, Arctium lappa (Burdock), Brassica juncea (Mustard leaf), Pteridium aguilinum (Bracken) and Chrysanthemum coronarium (Crown daisy) caused a significant induction of quinone reductase activity with a limited increase in arylhydrocarbon hydroxylase activity. Combination of crown daisy with burdock had synergistic effect on quinone reductase induction. Quinone reductase-inducing activity was found mostly in hexane and ethylacetate fractions of MeOH extract of crown daisy while it was not detected in n-butanol and water fractions. Animal study using SD rats demonstrated that crown daisy intake induced quinone reductase activity in liver, kidney, lung, and small intestine, confirming the presence of potent QR inducer(s) in crown daisy. These data suggest that some vegetables including crown daisy induced QR merits further investigation as a potential cancer preventive agent in human.
Effect of pulverizing method on the particle size of matured silkworm powder
Kim, Su-Bae,Kim, Kee-Young,Ji, Sang-Deok,Kim, Seong-Wan,Kim, Nam-Suk,Jo, You-Young,Kim, Jong-Gil,Kim, Young-Guk,Koo, Hui-Yeon,Moon, Hyung-Chul,Seok, Young-Seek,Lee, Hyun-Tai Korean Society of Sericultural Science 2018 International Journal of Industrial Entomology Vol.37 No.2
Recently matured silkworm powder was developed by RDA. In this study, the effect of pulverizing method on the particle size of matured silkworm powder was examined. FESEM was performed to observe the morphology and to measure the particle size of silkworm powder. Particle morphology of air-jet mill pulverized powder was round and smooth, however, those of roller-mill and hammer-mill pulverized mature silkworm was more harsh and square. Particle size was varied with pulverizing technique as follows; $1.1{\mu}m$ (air-jet mill), $10{\mu}m$ (roller mill), and $120{\mu}m$ (hammer mill), respectively. A proximate analysis results of air-jet mill powder showed that crude protein, crude lipid, crude fiber, and ash was 73%, 12%, 1.95%, and 3.4%, respectively. According to our results, air-jet mell technique might be used to make a tiny matured silkworm powder.