A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

대한방사선종양학회지 게재 논문의 통계적 오류 현황

박희철(Hee Chul Park),최두호(Doo Ho Choi),안성복(Song-Vogue Ahn),강진오(Jin Oh Kang),김은석(Eun-Seog Kim),박원(Won Park),안승도(Seung Do Ahn),양대식(Dae Sik Yang),윤형근(Hyong Geun Yun),정은지(Eun Ji Chung),지의규(Eui Kyu Chie),표홍렬( 대한방사선종양학회 2008 Radiation Oncology Journal Vol.26 No.4

목 적: 본 연구는 대한방사선종양학회지 게재 논문의 통계 오류 현황을 파악하고 이에 근거한 문제 제기를 통해 학회지의 학술적 발전에 기여하고자 하였다. 대상 및 방법: 2006∼2007년 사이에 대한방사선종양학회지에 게재된 총 77편의 논문을 연구 대상으로 하였다. 각각의 논문에 적용된 통계 방법론의 적정성 평가는 통계점검표를 활용하였다. 통계점검표에는 연구의 종류, 통계기법의 사용 범위, 각 논문에 적용된 통계 기법의 종류, 통계적 기법 적용의 타당성 항목이 포함되었다. 통계 오류는 ‘생략의 잘못’과 ‘시행의 잘못’ 항목으로 나누었다. 한 논문에서 서로 다른 항목이 여러 가지 관찰된 경우 각각 횟수로 측정하였다. 같은 항목이 2회 이상 측정된 경우 1회로 횟수를 측정하였다. 통계 전문가가 개별 논문을 대상으로 통계점검표를 작성하였다. 일차 평가자가 방사선종양학 전문가가 아닌 것에서 올 수 있는 평가 오류가 있을 수 있으므로 개별 통계점검표는 간행위원회에서 한 차례 더 세부 점검을 하였다. 작성된 통계점검표의 통계 분석은 SAS (version 9.0, SAS Institute, NC, USA) 소프트웨어를 이용하였고 빈도분석을 시행하여 각 항목의 빈도와 백분율을 산출하였다. 결 과: 총 77편 중 원문을 다운로드 할 수 없었던 4편을 제외한 73편의 게재 논문을 대상으로 평가하였다. 증례보고는 5편, 원저 논문은 68편이었다. 대상 논문 중 46편의 논문에서 통계적 추론을 사용하였고, 16편의 논문에서 단순한 기술통계를 사용했으며, 11편의 논문에서는 통계적 기법을 사용하지 않았다. 추론통계를 사용한 46편의논문에서 사용된 추론통계의 횟수는 각각 분할표분석 17회(37.0%), 비교통계분석 23회(50.0%), 회귀분석 7회(15.2%), 상관분석 5회(10.9%), 생존분석이 27회(58.7%)였다. 통계 기법을 활용하여 연구 결과를 분석할 때 통계적용의 오류가 없는 논문은 19%였다. ‘생략의 잘못’은 34편(50.0%)의 논문에서 총 50회 관찰되었다. ‘시행의 잘못’은 35편(51.5%)의 논문에서 총 47회 관찰되었다. ‘생략의 잘못’과 ‘시행의 잘못’이 모두 발견된 논문은 21편 (30.9%)이었다.결 론: 대한방사선종양학회지에 게재된 논문에서 통계 분석 과정의 다양한 영역에 걸쳐 크고 작은 통계적 오류가 있음을 확인하였다. 향후 대한방사선종양학회지 투고 논문의 심사 과정에서 통계 오류에 관한 적절한 심사를 추가하는 것이 필요하다고 판단된다. Purpose: To improve the quality of the statistical analysis of papers published in the Journal of the Korean Society for Therapeutic Radiology and Oncology (JKOSTRO) by evaluating commonly encountered errors. Materials and Methods: Papers published in the JKOSTRO from January 2006 to December 2007 were reviewed for methodological and statistical validity using a modified version of Ahn’s checklist. A statistician reviewed individual papers and evaluated the list items in the checklist for each paper. To avoid the potential assessment error by the statistician who lacks expertise in the field of radiation oncology; the editorial board of the JKOSTRO reviewed each checklist for individual articles. A frequency analysis of the list items was performed using SAS (version 9.0, SAS Institute, NC, USA) software. Results: A total of 73 papers including 5 case reports and 68 original articles were reviewed. Inferential statistics was used in 46 papers. The most commonly adopted statistical methodology was a survival analysis (58.7%). Only 19% of papers were free of statistical errors. Errors of omission were encountered in 34 (50.0%) papers. Errors of commission were encountered in 35 (51.5%) papers. Twenty-one papers (30.9%) had both errors of omission and commission. Conclusion: A variety of statistical errors were encountered in papers published in the JKOSTRO. The current study suggests that a more thorough review of the statistical analysis is needed for manuscripts submitted in the JKOSTRO.

무산소-호기 반응기의 유기물과 질소 제거모델 개발

안송엽,김환홍,소재철,권희태 원광대학교 2001 論文集 Vol.26 No.-

본 논문은 무산소-호기 유동상 반응기에서 폐수의 유기물과 질소제거에 관한 것을 나타내었으며, 이 공정은 두 개의 반응기 안에서 질산화와 탈질화로 얻어지는 제거율 결과를 모형화하여 검토하였다. 무산소조의 미생물 농도가 모형식으로 표현되면 -2079.898V2 - 202.2029(H/A) + 1123이고, BOD removal = -0.00613 HRT2 + 0.4014HRT + 46.993 and NO3-N removal = -0.0029 HRT2 + 0.1872 HRT + 77.45 로 각각 표현되어진다. 이러한 실험치는 모형치와 비슷한 값을 가진다고 할 수 있으며, 이 결과는 다른 방법에도 적용이 가능하다고 판단된다. This paper aims to get rid of BOD and nitrogen in wastewater as the anoxic-aerobic fluidized bed. The process for the removal of nitrogen consists of nitrification and denitrification in two reactors. The estimated formula for, Anoxic are Biomass concentration = -2079.898V2- 202.2029(H/A) + 1123, BOD removal = -0.00613 HRT2 + 0.4014HRT + 46.993 and NO3-N removal = -0.0029 HRT2 + 0.1872 HRT + 77.45. The experimental values are similar to values of estimated formula. Therefore, this result is able to adapt the other cases.

토당귀 종자의 형태적 특성과 발아와 입모율에 대한 저장방법,생장조절제 및 프라이밍 처리 효과

안희정,안복주,·김도현,·안영섭,·김영국,박춘근,이상원,·박충범·차선우,·송범헌, 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was conducted to have basic and applied informations to establish the cultivation method and to improve the cultivation techniques of Angelica gigas Nakai through investigating the rates of germination and seedling establishment with treatment of plant growth regulator and seed priming. The germination rates of round shape seed were about 0.3~12% higher than those of parallel shape. The germination rates with different storage durations were increased at the level of 25oC and 4oC with the storage days, while they were clearly higher at -20oC compared to those at 4oC and 25oC without the storage durations. With the results of germination rates with treating of plant growth regulator and seed priming, their rates with the round shape seeds were comparatively higher about 6% than those with parallel shape seed and they were increased with increasing of the storage durations. The germination rates were generally appeared higher with GA3 than did those with seed priming. The highest germination rate was appeared at 50 ppm GA3 of plant growth regulator and at -0.5 MPa PEG6000 of seed priming. The germination rates?ㅤ ?ㅤ of Angelica gigas were different with different temperature, storage durations and the treatments of plant growth regulator and seed priming. These results could be used according to different cultivating conditions.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

유동상에 의한 합성하수의 처리 특성

안송엽,권희태 圓光大學校大學院 2000 論文集 Vol.24 No.-

유동상은 반응기 형태의 변화에 따라 설계인자 및 운전 효율이 크게 변화하는 데, 효율적 운전을 위해서는 영향인자 간의 상호관계를 파악하는 것이 대단히 중요한 문제이다. 미생물막의 형성은 상향유속의 영향이 큰 것으로 나타났으며 최적의 상향유속은 1.17cm/sec∼1.32cm/sec로 나타났다. 각 반응기의 형태, 즉 A/H비에 관계없이 최대의 처리효율을 보이는 media 충전량은 18%이었고, BOD 부하 4.5kg-BOD5/㎥·d에서 상향유속은 1.32cm.sec이었다. 1.17cm/sec이하의 낮은 상승유속에서는 처리효율을 저하시킬 수 있는 bioparticles의 합체현상이 발생함을 알 수 있었다. A/H비에 따라 최대 처리효율은 94∼92%로 나타나므로, A/H비가 처리율에 영향을 준다는 것을 알 수 있었다. Bioparticles의 합체현상으로 인한 처리율의 감소를 막을 수 있는 최소유량은 A/H비에 따라서 다르게 나타난다. Process intensification without any increase in bed requires the exploitation of fluid mechanical phenomena as the basis for elegant solutions to the process engineering problems which result from the need to retain and control the immobilized biomass, and for biomass recovery. The fluidized bed biological reactor provides a solution to these needs. The wastewater treatment characteristics of the fluidized bed was filled with sand media. Indirect aeration were studied experimentally. The optimal range of velocities was 1.17cm/sec∼1.32cm/sec, and under the BOD loading condition of 4.5kg-BOD5/㎥·d, the media concentration appeared to 18% and surface upflow velocity also 1.32cm/sec for the maximum BOD removal. The BOD loading and F/M ratio condition of some of the 'steady-state' reactor, a degree would be expected. The medium effluent unfiltered BOD5 was 92%∼94%, at an HRT of 32 minutes.

배지의 무기염류와 sucrose 농도가 감자의 기내 shoot 증식과 기내경 삽목묘의 생존율에 미치는 영향

김희곤,임순희,안장순,송창길,박미경,강봉균 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

감자의 virus-free 기내경 삽목묘의 효율적인 대량 생산 체계의 확립을 위하여 생장점 배양으로 얻어진 감자의 기내경의 증식과 기내경의 순화삽목을 실시한 결과는 다음과 같다. 초대배양에서는 염류농도와 sucrose 농도가 높을수록 양호하였다. 즉, MS나 2MS에 sucrose를 6% 첨가한 배지에서 튼튼한 기내경이 생산되었다. 계대배양에서는 sucrose 농도가 높을수록 줄기의 길이는 단축되고 굵기는 커졌으며 생체중은 염류농도와 관계없이 3%의 sucrose가 첨가된 배지에서 무거웠다. 이들 유식물체의 경절을 삽목한 결과 계대배양 배지의 sucrose농도가 6%인 배지에서 생존율이 높은 경향이었다. In order to establish a mass propagation system of potato planting material, in vitro multiplication of virus-free shoot originated from meristem culture and sucrose concentrations on survival of cutting of the in vitro stem tried with various media containing different levels of MS salts(0.5x, 1x and 2x) and sucrose(1.0, 3.0, 6.0%), and the effects of MS salts elements were evaluated. Higher level of sucrose in the initial culture media favored the shoot growth, that is, both of 1MS and 2MS media containing 6.0% sugar produced stouter shoots. Higher level of sucrose in the subculture media also favored the development of compact stout shoots, but biomass production in terms of fresh weight was high on the media containing 3.0%sucrose, regardless of MS salts concentration. The survival rate of cuttings made of the in vitro stem segments tended to be higher in those obtained from subculture media containg 6.0%sucrose.

가정과 교육실습 프로그램 운영 개선에 대한 사례 연구

유태명,안영희,이송자 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.1

This study employs a case study method to examine the current implementation of student teaching program in a particular teacher education institution and its affilated school. The purpose ofthis sutdy is to improve the quality ofhome economics student teaching proghram by promoting collaboration of teacher education institution and host schools. Following five research problems are addressed: (1) Examining the problems ofstudent teaching from the literature review; (2) Identifying the problems of student teaching recognized by home economics student teachers; (3) Identifying the problems of student teaching and plans for improvements suggested in the seminar held for home economics teacher; (4) Analyzing and comparing the differences of opinion between home economics student teachers and student teaching cooperating home economics teachers on the student teaching program; and (5) Suggesting alternative implementation plans for improving student teaching program by promoting collaboration of teacher education institution and host schools.

球形 글라스비즈의 흐름角 測定

姜錫浩,宋希準,安熙官 嶺南大學校 工業技術硏究所 1978 연구보고 Vol.6 No.1

In order to study the flow characteristics of spherical glass-beads ranging from 40 mesh to-115 mesh, its angle of repose and angle of rupture were measured as a function of average particle size, addition of fine powder as glidant or lubricant, and admixture of 2 or 3 adjacent fractions of beads classified by screening. The angle of repose of the glass-beads increases with increasing particle size of above 100 mesh, but for beads smaller than 100 mesh, the angle of repose increases with decreasing particle size. Fine glidant of montmorcalite added to the beads in very small concentrations of 0.6wt, % decreases the flow angles, while it tends to increase strongly the flow angles at higher concentrations.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.