TGF-β1 Protein Expression in Non-Small Cell Lung Cancers is Correlated with Prognosis

Huang, Ai-Li,Liu, Shu-Guang,Qi, Wen-Juan,Zhao, Yun-Fei,Li, Yu-Mei,Lei, Bin,Sheng, Wen-Jie,Shen, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

To investigate the expression intensity and prognostic significance of TGF-${\beta}1$ protein in non-small cell lung cancer (NSCLC), immunohistochemistry was carried out in 194 cases of NSCLC and 24 cases of normal lung tissues by SP methods. The PU (positive unit) value was used to assess the TGF-${\beta}1$ protein expression in systematically selected fields under the microscope with Leica Q500MC image analysis. We found that the TGF-${\beta}1$ PU value was nearly two-fold higher in NSCLC than in normal lung tissues (p=0.000), being associated with TNM stages (p=0.000) and lymph node metastases (p=0.000), but not to patient age, gender, smoking history, tumor differentiation, histological subtype and tumor location (P>0.05). Univariate analysis indicated that patients with high TGF-${\beta}1$ protein expression and lymph node metastases demonstrated a poor prognosis (both p=0.000,). Multivariate analysis showed that TGF-${\beta}1$ protein expression (RR = 2.565, p=0.002) and lymph node metastases (RR=1.874, p=0.030) were also independent prognostic factors. Thus, TGF-${\beta}1$ protein expression may be correlated to oncogenesis and serve as an independent prognostic biomarker for NSCLC.

Causal Relations between Exposome and Stroke: A Mendelian Randomization Study

Hong-Qi Li,Yi-Wei Feng,Yu-Xiang Yang,Xin-Yi Leng,Prof Can Zhang,Shi-Dong Chen,Kevin Kuo,Shu-Yi Huang,Xue-Qing Zhang,Yi Dong,Xiang Han,Xin Cheng,Mei Cui,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

Background and Purpose To explore the causal relationships of elements of the exposome with ischemic stroke and its subtypes at the omics level and to provide evidence for stroke prevention. Methods We conducted a Mendelian randomization study between exposure and any ischemic stroke (AIS) and its subtypes (large-artery atherosclerotic disease [LAD], cardioembolic stroke [CE], and small vessel disease [SVD]). The exposure dataset was the UK Biobank involving 361,194 subjects, and the outcome dataset was the MEGASTROKE consortium including 52,000 participants. Results We found that higher blood pressure (BP) (systolic BP: odds ratio [OR], 1.02; 95% confidence interval [CI], 1.01 to 1.04; diastolic BP: OR, 1.03; 95% CI, 1.01 to 1.05; pulse pressure: OR, 1.03; 95% CI, 1.00 to 1.06), atrial fibrillation (OR, 1.18; 95% CI, 1.13 to 1.25), and diabetes (OR, 1.13; 95% CI, 1.07 to 1.18) were significantly associated with ischemic stroke. Importantly, higher education (OR, 0.69; 95% CI, 0.60 to 0.79) decreased the risk of ischemic stroke. Higher systolic BP (OR, 1.06; 95% CI, 1.02 to 1.10), pulse pressure (OR, 1.08; 95% CI, 1.02 to 1.14), diabetes (OR, 1.28; 95% CI, 1.13 to 1.45), and coronary artery disease (OR, 1.58; 95% CI, 1.25 to 2.00) could cause LAD. Atrial fibrillation could cause CE (OR, 1.90; 95% CI, 1.71 to 2.11). For SVD, higher systolic BP (OR, 1.04; 95% CI, 1.00 to 1.07), diastolic BP (OR, 1.06; 95% CI, 1.01 to 1.12), and diabetes (OR, 1.22; 95% CI, 1.10 to 1.36) were causal factors. Conclusions The study revealed elements of the exposome causally linked to ischemic stroke and its subtypes, including conventional causal risk factors and novel protective factors such as higher education.

Magnetoresistance Effect in Co<SUB>x</SUB>(Alq₃)<SUB>1-x</SUB> Granular Films

Gang Ni,Qi Shu,Xiaomeng Zhao,Junqi Wu,Peng Sheng,Jianfang Yin,Bin Ma,Wei Huang,Youwei Du 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Laboratory evaluation of differential attraction of Culex pipiens pallens to the volatiles of flowers, fruits, and seed pods

Bao-Ting Yu,Shu-Qi Huang,Yan-Mei Ding,Hatem Fouad,Hong-Jie Li,Jian-Chu Mo 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

The attraction of Culex pipiens pallens Coquillett to various potential sugar sources in their habitats remains unknown. In the present study, we evaluated the attraction of Cx. pipiens pallens to the volatiles of different kinds of flowers, fruits, and seed pods by multiple choice tests under laboratory conditions. Nine of the 21 tested fresh tissues of flowers were attractive to females and six of them were attractive to males. The volatiles of six flowers (Ligustrum quihoui, Abelia chinensis, Ophiopogon japonicus, Phaseolus vulgaris, Osmanthus fragrans, and Nerium indicum) were attractive to both female and male Cx. pipiens pallens, and the flowers of Torenia fournieri and Lavandula angustifolia were potentially repellent to female and male mosquitoes, respectively. Besides, the volatiles of three fruits and seed pods (Broussonetia papyrifera, Amygdalus persica, and Cucumis melo) were attractive to both female and male Cx. pipiens pallens while the seed pods of Ficus carica and Pyrus bretschneideri were only attractive to females and males, respectively. This study provides valuable information on developing novel control methods against Cx. pipiens pallens in China.

QTLs of Cold Tolerance-Related Traits at the Booting Stage for NIL-RILs in Rice Revealed by SSR

Ya Wen Zeng,Shu Ming Yang,Hong Cui,Xiao Juan Yang,Li Ming Xu,Juan Du,Xiao Ying Pu,Zi Chao Li,Zai Quan Cheng,Xing Qi Huang 한국유전학회 2009 Genes & Genomics Vol.31 No.2

QTLs for cold tolerance-related traits at the booting stage using balanced population for 1525 recombinant inbred lines of near-isogenic lines (viz. NIL-RILs for BC5F3 and BC5F4 and BC5F5) over 3 years and two locations by backcrossing the strongly cold-tolerant landrace (Kunmingxiaobaigu) and a cold-sensitive cultivar (Towada) was analyzed. In this study, 676 microsatellite markers were employed to identify QTLs conferring cold tolerance at booting stage. Single marker analysis revealed that 12 markers associated with cold tolerance on chromosome 1, 4 and 5. Using a LOD significance threshold of 3.0, compositive interval mapping based on a mixed linear model revealed eight QTLs for 10 cold tolerance-related traits on chromosomes 1, 4, and 5. They were tentatively designated qCTB-1-1, qCTB-4-1, qCTB-4-2, qCTB-4-3, qCTB-4-4, qCTB-4-5, qCTB-4-6, and qCTB-5-1. The marker intervals of them were narrowed to 0.3-6.8 cM. Genetic distances between the peaks of the QTL and nearest markers varied from 0 to 0.04 cM. We were noticed in some traits associated cold tolerance, such as qCTB-1-1 for 5 traits (plant height, panicle exsertion, spike length, blighted grains per spike and spikelet fertility), qCTB-4-1 for 8 traits (plant height, node length under spike, leaf length, leaf width, spike length, full grains per spike, total grains per spike and spikelet fertility), qCTB-4-2 for 3 traits (spike length, full grains per spike and spikelet fertility), qCTB-5-1 for 5 traits (plant height, panicle exsertion, blighted grains per spike, full grains per spike and spikelet fertility). The variance explained by a single QTL ranged from 0.80 to 16.80%. Three QTLs (qCTB-1-1, qCTB-4-1, qCTB-4-2) were detected in two or more trials. Our study sets a foundation for cloning cold-tolerance genes and provides opportunities to understand the mechanism of cold tolerance at the booting stage.

Preparation of silver Nano-Crystal patterns in oxide glasses under electric field accompanied by heat treatment

Jian Lin,Wen-Hai Huang,Shu-Hua Lei,Chang-Cheng Liu,Qi Bian,Li-Na Ma,Dong-Wei Li 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.4

Nano-crystals embedded in glass always have interesting quantum effects, while nano-crystal patterns may have wide potential applications in preparing PIC, OEIC and other types of electronic and optoelectronic devices. A direct current electric field, accompanied by heat treatment, was applied on tellurite or silicate glass sheets. The silver ions in a diffused film were introduced into the glass matrix under a high-voltage electric field, instead of supplied during glass melting. The direct diffusion of metal ions, the nucleation and crystallization of nano-crystals in the glass were well controlled by the electric field accompanied by heat treatment. It was found that silver nano-crystal patterns can be transfer printed into tellurite and silicate glass sheets with both patterned diffusion and patterned crystallization. Nano-crystals embedded in glass always have interesting quantum effects, while nano-crystal patterns may have wide potential applications in preparing PIC, OEIC and other types of electronic and optoelectronic devices. A direct current electric field, accompanied by heat treatment, was applied on tellurite or silicate glass sheets. The silver ions in a diffused film were introduced into the glass matrix under a high-voltage electric field, instead of supplied during glass melting. The direct diffusion of metal ions, the nucleation and crystallization of nano-crystals in the glass were well controlled by the electric field accompanied by heat treatment. It was found that silver nano-crystal patterns can be transfer printed into tellurite and silicate glass sheets with both patterned diffusion and patterned crystallization.