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Bioelectrical Impedance May Predict Cell Viability During Ischemia and Reperfusion in Rat Liver
Mei Lan Cui,안현수,김종연,신현진,이동식,김홍진,윤성수 대한의학회 2010 Journal of Korean medical science Vol.25 No.4
Ischemia and reperfusion (I/R) injury is a major cause of hepatic failure after liver surgery, but no method could monitor or predict it real-time during surgery. We measured bioelectrical impedance (BEI) and cell viability to assess the usefulness of BEI during I/R in rat liver. A 70% partial liver ischemia model was used. BEI was measured at various frequencies. Adenosine triphosphate (ATP) content, and palmitic acid oxidation rate were measured, and histological changes were observed in order to quantify liver cell viability. BEI changed significantly during ischemia at low frequency. In the ischemia group, BEI increased gradually during 60 min of ischemia and had a tendency to plateau thereafter. The ATP content decreased below 20% of the baseline level. In the I/R group, BEI recovered to near baseline level. After 24 hr of reperfusion, the ATP contents decreased to below 50% in 30,60 and 120 min of ischemia and the palmitic acid metabolic rates decreased to 91%, 78%, and 74%, respectively, compared with normal liver. BEI may be a good tool for monitoring I/R during liver surgery. The liver is relatively tolerant to ischemia,however after reperfusion, liver cells may be damaged depending upon the duration of ischemia.
Cui, Mei Hua,Ok, Sung Han,Yoo, Kyoung Shin,Jung, Kwang Wook,Yoo, Sang Dong,Shin, Jeong Sheop Japanese Society of Plant Physiologists 2013 Plant & cell physiology Vol.54 No.1
<P>Arabidopsis thaliana Cell Growth Defect factor 1 (Cdf1) has been implicated in promotion of proapoptotic Bax-like cell death via the induction of reactive oxygen species (ROS). Here we report a conserved function of a chloroplast-targeting Cdf-related gene Responsive to Senescence (CRS) using CRS overexpression and loss of function in plants as well as CRS heterologous expression in yeast. CRS expression was strongly induced in senescent leaves, suggesting its main functions during plant senescence. CRS expression in yeast mitochondria increased the ROS level and led to cell death in a manner similar to Cdf1. In whole plants, overexpression of CRS caused the loss of chlorophylls (Chls) and the rapid onset of leaf senescence, while the lack of CRS led to the delay of leaf senescence in a loss-of-function mutant, crs. The higher and lower accumulation of H(2)O(2) was correlated with early and late senescence in CRS-overexpressing and crs mutant plants, respectively. Furthermore, expression of senescence-related marker genes and metacaspase genes was induced in CRS-overexpressing plants in response to dark. Our findings suggest that CRS plays a key role in the leaf senescence process that accompanies H(2)O(2) accumulation resulting in cell death promotion.</P>
Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma
Cui, Mei-Hua,Hou, Xiao-Lin,Lei, Xiao-Yan,Mu, Fang-Hong,Yang, Gui-Bin,Yue, Lin,Fu, Yi,Yi, Guo-Xing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.
Cui, Xue Mei,Lyoo, Won Seok,Son, Won Keun,Park, Dae Hun,Choy, Jin Ho,Lee, Taek Seung,Park, Won Ho IOP Publishing Ltd 2006 Superconductor science & technology Vol.19 No.12
<P>YBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7−δ</SUB> superconducting nanofibres were successfully fabricated via the electrospinning method in combination with the sol–gel process. The solution was prepared by the sol–gel process with a homogeneous aqueous PVA solution containing Y, Ba, and Cu acetates. The viscosity of the precursor sol for electrospinning was controlled by the evaporation of solvent and a condensation reaction. The electrospun nanofibres were pyrolysed to remove PVA or volatile components, and then sintered to generate a superconducting phase. The critical transition temperature (<I>T</I><SUB>c</SUB>) of superconducting YBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7−δ</SUB> nanofibres was measured by DC susceptibility tests. By optimizing the electrospinning process and following heat treatments, superconducting YBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7−δ</SUB> nanofibres with a <I>T</I><SUB>c</SUB> = 92.2 K could be produced. </P>
Dynamic burstiness of word-occurrence and network modularity in textbook systems
Cui, Xue-Mei,Yoon, Chang No,Youn, Hyejin,Lee, Sang Hoon,Jung, Jean S.,Han, Seung Kee Elsevier 2017 PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIO Vol.487 No.-
<P><B>Abstract</B></P> <P>We show that the dynamic burstiness of word occurrence in textbook systems is attributed to the modularity of the word association networks. At first, a measure of dynamic burstiness is introduced to quantify burstiness of word occurrence in a textbook. The advantage of this measure is that the dynamic burstiness is decomposable into two contributions: one coming from the inter-event variance and the other from the memory effects. Comparing network structures of physics textbook systems with those of surrogate random textbooks without the memory or variance effects are absent, we show that the network modularity increases systematically with the dynamic burstiness. The intra-connectivity of individual word representing the strength of a tie with which a node is bound to a module accordingly increases with the dynamic burstiness, suggesting individual words with high burstiness are strongly bound to one module. Based on the frequency and dynamic burstiness, physics terminology is classified into four categories: fundamental words, topical words, special words, and common words. In addition, we test the correlation between the dynamic burstiness of word occurrence and network modularity using a two-state model of burst generation.</P>
Depletion of BIS sensitizes A549 cells to treatment with cisplatin
Mei Nu Cui,윤혜현,이난이,김혜윤,임창임,김용삼,이정화 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.1
Bcl-2 interacting cell death suppressor (BIS), an anti-stress and ant-apoptotic protein, has been reported to be expressed at high levels in various cancers. In a previous study, we reported on a high level of expression of BIS in non small cell lung cancer tissues. To explore the significance this finding in lung cancer, in this study, we investigated the effect of BIS depletion on the survival of A549 cells upon treatment with anti-tumor agents. BIS knock out A549 cells, prepared by the CRISPR/Cas9 system, revealed a substantial decrease in survival to cisplatin treatment. Western blotting and quantitative real time PCR assays indicated that, among the anti-apoptotic Bcl-2 family proteins, the expression of Mcl-1 was decreased by BIS depletion at the protein level not at the mRNA level. Since BIS expression has been shown to be regulated by HSF1, we subsequently illustrated the sensitization effect of KRIBB11, a HSF1 inhibitor, on cisplatin-induced toxicity in A549 cells, accompanied by a decrease in both BIS and Mcl-1 expression. Our results suggest that BIS-mediated Mcl-1 stabilization represents a potential therapeutic target for cancer therapy.
Impaired Cation Transport May Lead to Bioelectrical Impedance Changes during Hepatic Ischemia
Mei Lan Cui,Hyun Soo Ahn,Jong Yeon Kim,Dong Shik Lee,Hong Jin Kim,Sung Su Yun 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.6
Purpose: Ischemia and reperfusion (I/R) injury is a major cause of hepatic failure after liver surgery, but there is no direct method to monitor it in real-time (like an electrocardiogram in heart disease) during surgery. Recently we found the possible role of bioelectrical impedance (BEI) to monitor I/R injury in liver. But the mechanism responsible for ischemia-related BEI changes has not been clearly determined. Methods: The authors used an LCR meter to quantify BEI changes at 0.12 ㎑. Livers were subjected to 70% partial ischemia for 120 minutes, and ATP contents, cation changes in extracellular fluid (ECF; determined using an in vivo intracellular microdialysis technique), hepatocyte sizes, and histological changes were then examined. Results: Liver tissue BEI was found to increase gradually during the first 60 minutes of ischemia and then tended to plateau. During the same period, intracellular ATP contents decreased to below 20% of the baseline level, [Na?] in ECF decreased from 150.4±3.8 to 97.8±10.6 mmol/L, and [K?] in ECF increased from 7.5±0.3 to 34.3±5.5 mmol/L during the first 60 minutes of ischemia. Hepatocyte diameter increased by ∼20% during the first 60 minutes of ischemia. Conclusion: This study suggests that BEI changes during hepatic ischemia are probably caused by sodium and potassium concentration changes in the ECF due to reduced intracellular ATP contents.