인광석의 인산비효증진에 관한 연구-토양중 mycorrhizae 포자밀도조사 및 인광석용해성사상균의 분리와 배양-

유장걸,김형옥,이신찬 제주대학교 1985 논문집 Vol.20 No.-

To make a survey on the distribution of V. A. mycorrhizae on Cheju-Island. 60 soil samples were taken from the cultivated and non-cultivated lands (27 volcanic soils and 33 non-volcanic soils). Spore densities (spore number/100g soil) were measured by the floating method Phosphate-solubilizing fungi were isolated from 18 soil samples and selected by the pure cultivation method on PDA-calcium phosphate media Adaptation cultivations of the selected fungi were conducted in the rock phosphate-liquid media succeesive- ly with the change of sugar to straw composition. 1. Low spore density below 100 was observed in 19 sampling sites and high density above 500 was shown in only five fields, rest of the locations being ordinary levels. It seemed that spore density was related to the locality but not to pH. water content, total nitrogen and phosphate percentage of the soil. 2. Phosphate solubilizing fungi were found on PDA-calcium phosphate media in 41 soil samples. 3. After 6-day pure cultivatin on PDA-media. fungi having 8-15mm of colony radii(R). 14-22mm of halo radii(H) and higher H/R ratio than 1.2. were obtained in 18 soil samples. 4. Five successive cultivations in the liquid media containing powdered rock phosphate were undertaken to adapt the selected fungi to the low sugar/straw condition (sugar 0.35%. straw 1.15%). At the end of 5 th cultivation, seven fungi (from soil No. 1. 8. 24, 26. 31, 43 and 45) remained and showed about 29 percentage of rock phosphate solubility making the liquid media neutral.

High-grade mucoepidermoid carcinoma in the thyroid gland with poor prognosis

Shin, Hyeong Chan Yeungnam University College of Medicine 2021 Yeungnam University Journal of Medicine Vol.38 No.2

Mucoepidermoid carcinoma (MEC) is the most common malignant neoplasm of the salivary gland, but primary thyroid MEC has rarely been reported and usually has a good prognosis. Herein, I report a case of thyroidal MEC with a poor prognosis in an 82-year-old woman with an anterior neck mass. Ultrasonography and computed tomography revealed a thyroid mass. The patient initially underwent fine-needle aspiration, was diagnosed with malignancy, and underwent a right lobectomy. On gross examination, a 4.0×3.6×2.6 cm-sized ill-defined, unencapsulated, and infiltrative tan to whitish mass with necrosis was identified. Microscopically, epidermoid tumor cell nests or solid sheets were identified. Mucous cells that were positive for periodic acid-Schiff and mucicarmine stains were also identified within epidermoid cell nests. Frequent mitosis and necrosis were observed. Immunohistochemical staining for p40 and p63 was positive, and that for thyroid transcription factor-1 and paired box gene 8 was focally positive. According to the Armed Forces Institute of Pathology grading system for salivary gland MEC, the current case was classified as high-grade MEC. After surgery, the patient suffered from dyspnea due to a remnant neck mass that compressed and obstructed the trachea; therefore, the patient refused further treatment. Thyroidal MECs are considered low-grade with a favorable prognosis, but there are several reported cases of thyroidal MEC with poor prognosis. The current case is a rare presentation of high-grade thyroidal MEC with a poor prognosis.

Effects of Concrete and Wood Building Environments on Pregnant Dams and Embryo-Fetal Development in Rats

Shin, In-Sik,Kim, Sung-Hwan,Lim, Jeong-Hyeon,Lee, Jong-Chan,Park, Na-Hyeong,Shin, Dong-Ho,Moon, Chang-Jong,Kim, Sung-Ho,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.4

We have recently reported that the continuous exposure of rats to a concrete building environment under cool temperatures had adverse effects on general health parameters and embryo-fetal development. This study examined to compare the potential effects of concrete and wood building environments on pregnant dams and embryo-fetal development in rats. Groups of 10 mated females were exposed to polycarbonate (control), concrete, or wood cages from gestational days (GD) 0 to 20 under cool temperatures $(11.9\sim12.3^{\circ}C)$. All the females underwent a caesarean section on GD 20, and their fetuses were examined for any morphological abnormalities. The temperatures in the cages were similar in all groups but the relative humidity in the concrete and wood groups were higher than in the control group. The concentration of volatile organic compounds in the wood group was higher than in the control group. In the concrete group, maternal effects manifested as an increase in the incidence of clinical signs, a lower body weight, and a decrease in the thymus and ovary weights. Developmental effects included increased post-implantation loss and decreased litter size. Infrared thermal analysis showed that the skin temperature of the rats in the concrete group was lower than that in the control group. In contrast, there were no exposure-related adverse effects on the maternal and developmental parameters in the wood group. Overall, the exposure of pregnant rats to a concrete building environment under cool temperatures has adverse effects on the clinical signs, body weight, skin temperature, organ weight, and embryo-fetal development. On the other hand, exposure to a wood building environment does not have any adverse effects in rats.

Reduction in Salmonella mutagenicity of mainstream cigarette smoke condensate by cation exchange chromatography

Shin, Han-Jae,Lee, Byeong-Chan,Sohn, Hyung-Ok,Park, Chul-Hoon,Lee, Hyeong-Seok,Yoo, Ji-Hye,Lee, Dong-Wook,Hyun, Hak-Chul The Korean Society of Tobacco Science 2008 한국연초학회지 Vol.30 No.2

Mutagenicity of cigarette smoke is one of the major health concerns related to smoking. Reduction of the components comprising mutagenic activity in cigarette mainstream smoke can be expected to bring about reduced risk of smoking. The purpose of this study is to isolate mutagenic compounds and to investigate the relative contribution to allover mutagenicity of smoke to find clues for the effective elimination of the components. Cigarette smoke condensate (CSC) was obtained from total particulate matter (TPM) of mainstream smoke, and several fractions fractionated from CSC were made by combination of cation exchange chromatograph and reverse-phase chromatography. The mutagenic activity of these fractions was assessed using Salmonella mutagenicity assay with S. typimurium TA98 strain in the presence of metabolic activation system (S-9). The fractions isolated by cation exchange and reverse-phase column showed relatively high mutagenic activity. The basic and hydrophilic fraction 9 showed approximately 33% of mutagenic activity of CSC and its specific activity was 2,459 revertants/mg TPM. These results suggest that hydrophilic cation exchanger and/or other adsorbents possessing similar properties may be used to remove the mutagenic compounds from mainstream smoke.

Anti-atherosclerosis and hyperlipidemia effects of herbal mixture, Artemisia iwayomogi Kitamura and Curcuma longa Linne, in apolipoprotein E-deficient mice

Shin, Hyun-Soo,Han, Jong-Min,Kim, Hyeong-Geug,Choi, Min-Kyung,Son, Chang-Gue,Yoo, Ho-Ryong,Jo, Hyun-Kyung,Seol, In-Chan Elsevier 2014 Journal of Ethnopharmacology Vol.153 No.1

<P>Artemisiaiwayomogi Kitamura and Curcuma longa Linne. (ACE) has been popularly used to treat atherosclerosis as well as hyperlipidemia in the Asian countries.</P>

Initial Response of Osteoblast-Like Cells on Magnesium Ion Implanted Titanium Surface

( Hyeong Joo Shin ),( Dae Gon Kim ),( Chan Jin Park ),( Lee Ra Cho ),( Hee Su Lee ),( Eung Sun Byon ),( Yong Soo Jeong ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.3

The interactions between the implant surface and surrounding tissue are important for successful osteogenesis. Biomechanical interlocking and biochemical bonding are believed as to be the mechanism of osseointegration involving the initial osteoblast attachment, proliferation and differentiation. The purpose of this study is to evaluate the MC3T3-E1 cell responses to a magnesium (Mg) ion implanted titanium surface produced using a plasma source ion implantation method. Commercially pure titanium discs were prepared to produce four different surfaces; ground surface, Mg-implanted on ground surface, sandblasted surface, and Mg-implanted on sandblasted surface. Cell attachment, proliferation and differentiation of MC3T3-El cells were evaluated. Scanning electron microscopy and confocal laser microscopy revealed more cell proliferation on the Mg ion groups than on the non Mg ion groups. On the ground surface, cells showed some degree of contact guidance in alignment with the grooves. The MTT assay to determine the cellular viability and proliferation showed a higher optical density on the Mg ion groups than on the non Mg ion groups. This suggests that a Mg ion implanted titanium surface induces a better initial cellular response than the non Mg ion groups.

Effects of 1,3-Dichloro-2-propanol on Pregnant Dams and Embryo-Fetal Development in Rats

Na-Hyeong Park,Jong-Chan Lee,In-Sik Shin,Kang-Hyun Kim,Changjong Moon,Sung-Ho Kim,Jong-Choon Kim 한국생명과학회 2008 한국생명과학회 심포지움 Vol.49 No.-

Evaluation of Maternal Toxicity in Rats Exposed to 1,3-Dichloro-2-propanol during Pregnancy

Jong-Chan Lee,In-Sik Shin,Gang-Hyeon Kim,Na-Hyeong Park,Chang-Jong Moon,Chun-Sik Bae,Sung-Soo Kang,Sung-Ho Kim,Dong-Ho Shin,Jong-Choon Kim 한국독성학회 2008 Toxicological Research Vol.24 No.4

The present study was carried out to investigate the potential adverse effects of 1,3-dichloro-2-propanol on pregnant dams after maternal exposure during the gestational days (GD) 6 through 19 in Sprague-Dawley rats. The tested chemical was administered orally to pregnant rats at dose levels of 0, 10, 30, or 90 ㎎/㎏/day. During the test period, clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights, and Caesarean section findings were examined. In the 90 ㎎/㎏ group, decreases in the body weight gain and food consumption, and increases in the weights of liver and adrenal glands were observed. Serum biochemical investigations revealed increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol (CHO), triglyceride (TG), alkaline phosphatase (ALP), and bilirubin (BIL) and decreases in glucose (GLU), albumin (ALB) and total protein (TP). In the 30 ㎎/㎏ group, a decrease in the food consumption and an increase in the liver weight were observed. Serum biochemical investigation also showed increases in CHO and TG and a decrease in glucose. Since there were no signs of maternal toxicity in the 10 ㎎/㎏ group, it is considered to be the no-observed-adverse-effect level (NOAEL) of 1,3-dichloro-2-propanol. It is concluded that successive oral administration of 1,3-dichloro-2-propanol to pregnant rats for 14 days may cause significant toxicities in body weight and liver at a dose rate ≥ 30 ㎎/㎏/day.

태생기 단백영양결핍 백서에서 췌장 베타세포의 기능 및 발달

박형규,김성길,박도준,신찬수,박경수,김성연,이홍규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1

연구배경:많은 역학연구에서 태생개의 영양결핍이 성인이 된 후 내당능장애 또는 당뇨병의 발생과 관련되어 있음이 관찰되었다. 이러한 연관성은 태생기의 영양결핍이 자궁 내 성장을 저하시키고, 포도당 대사를 조절하는 조직의 발달 및 기능에 장기적인 변화를 유발한 결과로 설명되어 왔다. 이에 저자들은 태생기의 단백질 영양결핍이 췌장 베타세포의 기능 및 발달에 미치는 영향을 알아보고자 하였다. 방법:저단백 식이를 먹인 어미쥐에서 태어난 수컷쥐를 대상으로 이유 후 정상식이(회복군)또는 저단백식이(저단백군)를 먹여 생후 25주가 되었을 때, 정맥당부하검사 및 췌장의 형태학적 분석을 시행하여 정상 대조군과 비교하였다. 결과:태생기에 단백질 영양결핍을 겪은 백서는 대조군에 비해 체중이 유의하게 적었으며, 생후 정상식이로 회복시켜 주어도 체중은 회복되지 않았다. 당부하검사에서 포도당 내성은 서로 차이가 없었다. 포도당 주사 후 20분의 인슐린 농도는 회복군(0.24±0.03nmol/L)및 저단백군(0.28±0.02nmol/L)이 대조군(0.43±0.05nmol/L)에 비해 유의하게 낮았다(p<0.05). 당부하검사 중 인슐린 곡선하면적은 회복군 및 저단백군(0.39±0.03nmol/L/min, 0.42±0.02nmol/L/min)에서 대조군(0.54±0.03nmol/L/min)에 비해 유의하게 감소하였으며(p<0.01), 특히 후기 인슐린 분비반응에서 심한 장애가 관찰되었다. 췌장무게 및 체중에 대한 췌장 무게의 비율은 회복군 및 저단백군에서 대조군에 비해 유의하게 감소되어 있었다(p<0.01). 췌장 세포에 대한 베타세포의 비율은 저단백군(0.91±0.14%)이 대조군(21.9±0.23%)및 회복군(1.79±0.25%)에 비해 유의하게 감소되어 있었다(p<0.05). 체중에 대한 베타세포 양은 저단백군(13.2±3.2㎍/g body weight)에서 대조군(34.8±5.6㎍/g body weight)에 비해 유의하게 적었다(p<0.05). 결론:태생기의 단백질 영양결핍은 췌장 베타세포의 지속적인 발달장애 및 인슐린 분비능의 저하를 유발하였으며, 이는 태생기의 단백질 영양결핍이 다른 유해한 환경적 또는 유전적 조건과 함께 성장 후 당뇨병의 발생에 기여할 가능성을 시사한다. Background:Nutritional deprivation of the fetus and infant may be associated with susceptibility to impaired glucose tolerance or type 2 diabetes in adult life. This association has been interpreted as a long­term effects of nutritional factors that reduce fetal growth and impair the development of tissues that regulate glucose metabolism. This study aimed to investigate the effect of protein malnutrition in a fetus and early life on the pancreatic β­cell function and development. Methods:Sprague­Dawley rats were fed a low­protein(8% casein)diet during pregnancy and lactation. Their male offspring were weaned onto either a control(18% casein)diet(recuperated group, R)or a low­protein diet( low­protein group, LP). The offspring of the rats control diet were weaned onto the pancreas were performed to evaluate the pancreatic β­cell function and development at the 25th week of age. Results:Offspring of the protein­malnourished rats had a significantly lower body weights than the controls. The R and LP showed no major impairment in glucose tolerance, but the plasma insulin concentrations in the R(0.24±0.03nmol/L)and LP(0.28±0.02nmol/L )groups were lower at 20 min during IVGTT than the C(0.43±0.05nmol/L )groups. The areas under the curve for insulin(AUC insulin)during IVGTT were significantly lower in R and LP(0.39±0.03nmol/L/min, 0.43±0.02nmol/L/min)groups than the C(0.54±0.03 nmol/L/min)group. In particular, the rats with fetal protein malnutrition showed severe impairment in late­phase insulin secretion to a glucose load. Both the pancreas weight and the proportion of the pancreas weight to the body weight were significantly lower in the R and LP groups than the C group. The proportion of β­cells to pancreatic cells was lower in the LP(0.91±0.14%)group than the C(2.19±0.23%)and R(1.79±0.25%)group. The relative β­cell mass was significantly lower in the LP(by 62%)group that the C group. Conclusion:Rats with fetal protein malnutrition showed persistently impaired pancreatic β­cell development and reduced insulin secretion capacity. These findings suggest that 2 diabetes in adult life along with other deleterious environment of genetic conditions(J Kor Diabetes Asso 26:21∼30, 2002).

The Effect of Different Sagittal Angles of the Tibial Guide on Aperture Widening of the Tibial Tunnel during Modified Transtibial Anterior Cruciate Ligament Reconstruction: A Randomized In Vivo Study

( Young Chan Kim ),( Tulyapruek Tawonsawatruk ),( Hyeong Hwa Woon ),( Ji Woong Yum ),( Myung Jin Shin ),( Rodolfo S. Bravo Jr ),( Kyung Wook Nha ) 대한슬관절학회 2017 대한슬관절학회지 Vol.29 No.1

Purpose: The effect of sagittal plane angle of the tibial tunnel on the severity of tibial intra-articular aperture expansion caused by iatrogenic rereaming in anterior cruciate ligament (ACL) reconstruction using a modified transtibial technique is unknown. The purpose of this study was to compare the severity of intra-articular aperture widening at different angles (40°, 45°, and 50°) of the tibial guide (TG). Materials and Methods: Ninety-seven patients who underwent modified transtibial ACL reconstruction were randomly allocated to TG 40°, 45°, and 50° groups. Intra-articular tibial aperture width (TW) and tibial tunnel length (TTL) were measured intraoperatively using an arthroscopic ruler and a depth gauge. Results: The TG 50° group had significantly greater tibial aperture widening than the TG 40° group. There was a significant difference among TG 40°, 45°, and 50° groups and the percentage of knees with TTL <35 mm was 8%, 9% and 3%, respectively. There were 2 females with TTL <35 mm in TG 40° and 45° groups each. The average mediolateral length of the tibial plateau was 75 mm. Conclusions: This study shows that the TG angle of 40° would reduce the severity of intra-articular aperture widening of the tibial tunnel compared to 45° or 50° in modified transtibial ACL reconstruction.