우리나라 간암 환자에서의 Cytochrome P450 2E1 유전자의 다형성에 관한 연구

백승운,윤정한,황유진,장유현,이효석,김정룡 大韓消化器病學會 1997 大韓消化器病學會誌 Vol.30 No.4

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.

정상과 만성염증성 활막배양세포에서 Corticotropin releasing hormone(CRH)이 cyclooxygenaase-1(COX-1)과 cyclooxygenase-2(COX-2) mRNA발현에 미치는 영향

정운원,이승관,이창규,조경진,김성욱,하주희,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Corticotropin-releasing hormone(CRH) is a major regulator of the hypothalamic-pituitaryadrenal axis(HPA). In inflammatory stress, the cytokines TNF-, IL-6 stimulate the production of CRH, a 41 amino acids neuropeptide, in the hypothalamus. The release of CRH leads to pituitary production of adrenocorticotropic hormone, followed by glucocorticoid secretion by the adrenal cortex. Glucocorticoids suppress namy components of the inflammatory process. Recently, CRH and CRH receptor were reported to be located in the periphery such as Immune system and chronic inflammatory sites as rheumatoid arthritis (RA). Cyclooxygenase consisted of two isoforms, COX-1 and COX-2, converts arachdonate to prostaglandins(PGs) which are important mediators of inflammation. insymoviocyte in RA, it was described that COX-2 mRNA was markedly increased by inflammatory agents, PMA or IL-1 and COX-1 transcripts were not modulated. We examined the modulation of COX by immune CRH in cultured normal and rheumatoid synoviocytes. Our results were shown that COX-1 mRNA expression decreased with the each stimulation of PMA and IL-1 in normal synoviocyte. In RA synoviocyte, PMA and IL-1 were increased mRNA expression of COX-1. In simultaneous treatment with CRH, PMA group was decreased, but IL-1 group was increased mRNA expression of COX-1. COX-2 mRNA expression was slightly increased by the treatment with PMA and highly increased by IL-1. After CRH treatment, PMA and IL-1 addictively increased COX-2 mRNA expression. We think that these results are contributed to the influence of increased cANP by CRH on the promoter of COX-2 in normal and synoviocytes. Because many cytokines, neuropeptides, and signal transduction pathways are involved in chronic inflammation, the exact role of CRH on inflammation is not fully elucidated. To achieve this goal, further experiments are needed.

부력기구를 활용한 수중운동 프로그램이 편마비 환자의 보행 동작에 미치는 효과

홍승범,조효구,정태운 용인대학교 특수체육연구소 2009 특수체육연구 Vol.6 No.-

본 연구는 부력기구를 활용한 수중운동 프로그램이 편마비 환자의 보행동작에 미치는 효과를 분석하는데 목적이 있다. 대상자는 S시 B복지관을 이용하고 독립보행이 가능하지만 수중운동 프로그램에 참여 경험이 없는 뇌졸중 좌측편마비 환자 21명중 7명을 무선표집법에 의해 선정하였다. 수중운동 프로그램을 실시 한 후 신체중심 이동, 보행 주기, 다리관절각, 다리관절 각속도의 자료를 분석하기 위하여 SPSS ver. 13.0 통계프로그램을 이용하여 대응표본 t-test를 실시하였다. 이상의 자료 분석 결과를 기초로 도출한 연구의 결론은 다음과 같다. 첫째, 보행주기는 환측의 입각기와 단일지지기의 비율이 향상되고, 환측과 건측의 비율 차이가 줄어들어 보행주기가 개선된 것으로 나타났다. 둘째, 신체중심의 이동은 거리가 늘어나고 시간은 줄어들어 속도가 빨라지는 결과는 수중운동프로그램이 편마비 환자의 보행 동작에서 신체중심 이동에 영향을 미친것으로 나타났다. 셋째, 관절각은 환측의 사전 측정결과 보다 관절각이 커지고 건측의 사전 측정결과 보다 작아져 수중운동 프로그램 참여 후에 환측과 건측의 좌우불균형이 개선된 것으로 나타났다. 넷째, 관절각속도는 보행주기에서 나타난 근력향상과 관절각 결과에서 나타난 관절가동범위 향상으로 인해 관절각속도 또한 긍정적인 결과를 보이며, 특히 모든 관절이 입각기 시 보다 유각기에서 각속도가 개선된 것으로 나타났다. The purpose of this research is to investigate the effects of the underwater exercise program that utilizes buoyancy appliances on the hemiplegics’ walking. The subjects are selected randomly among 21 patientswho have had been hemiplegic more than 5 years in B Rehabilitation Center in Seoul. The final 7 patients who had no exercise with left side hemiplegia were selected. After they have participated in 8-week underwater exercise program, central body movement, gait cycle, the angle of legs joints and the angular speed of leg joints are analyzed with paired t-test using statistics program, SPSS 13.0. Based on the analysis of collected data, this research makes following conclusions. First, while the subject is walking, there is increase in displacement for central body movement and decrease in time resuted in increase in speed. This result made the central body movement affected the gait. Second, Improvement in the ratio of stance phase to single needling support phase and decrease in the ratio difference between hemiplegic and normal side leads to improvement of gait cycle. Third, the disparity between hemiplegic and normal side have improved with increase of the angle of leg joint for hemplegic side and decrease of the normal side after participating in the underwater exercise program. Lastly, as a result of muscular strengthening from gait cycle and range increase of joint angle, angular speed also shows improvement, especially in the swing phase compared to stance phase.

악하부에 발생한 결핵성 경부 임파선염

김영운,정숭룡,이승호,이종호,김창룡,박문성,류선열 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.4

Tuberculosis, a chronic infectious granulomatous disease, is preumed to be quite a rare entity. Because of the development of chemotherapy and the improvement of nutritional conditions, incidence of tuberculosis have reduced. For these reasons, tuberculosis may be overlooked in the differential diagnosis whenever dealing with a submandibular swelling. Diagnosis of tuberculous lymphadenitis consists of historical data, physical findings, laboratory tests and histologic examination. The treatment of choice seems to be surgical excision and long term antituberculosis chemotherapy. Surgery provides a rapid tissue diagnosis, because the histological examination of the excisional biopsy is the most reliable diagnostic test. This is the report of a case of tuberculous cervical lymphadenitis on left submandibular area with no evidence of the involvement of lung.

유방 침윤성 관암종의 CD44 발현도와 예후 인자들과의 관계

김정완,곽정식,손윤경,이승철,정운복 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

CD44 is a family of transmembrane glycoproteins involved in cell-cell and cell-matrix interactions. Expression of CD44 isoforms (splice variants) has been shown to be associated with poor prognosis in several human cancers. We evaluated the expression patterns of the CD44 isoform (CD 44 splice variant v6) in infiltrating ductal carcinoma of the breast by immunohistochemical and RT-PCR method. Paraffin embedded blocks from seventy-five cases of mastectomized samples were analyzed immunohistochemically using monoclonoal antibody against CD44v6. CD44v6 was detected in fifty-seven cases(76%) of the tumor samples. Adjacent normal myoepithelial cells and ductal epithelial cells revealed focal positive reaction to CD44v6. Thirty-two cases (80.0%) withe lymph nodal metastasis revealed overexpression of CD44v6 monoclonal antibody, but twenty-five cases (71.4%) without nodal metastasis also showed positive reaction to CD44v6 monclonal antibody, and there is no statisticallly significant value. Other prognostic factors of infiltrating ductal carcinoma, such as tumor size, histologic grade and hormonal receptors did not show any significant correlation with CD44v6 expression. The RT-PCR studies for 9 cases of infiltrating ductal carcinoma showed the same band patterns both in the normal and tumor tissues. From the above results, it is concluded that the expression of CD44v6 is not a valuable prognostic marker of infiltrating ductal carcinoma of breast.(Korean J Pathol 1997;31: 635~643)

[논문]감천댐 건설에 따른 기상변화 연구

이화운,정우식,이귀옥,임헌호,백승주 부산대학교 환경문제연구소 2004 環境硏究報 Vol.22 No.-

In order to investigate the variation of meteological factors induced by construction of dam, the prediction model, WQRRS, is used. As a result, the effect of Dam construction showed that the amount of evaporation increases because of broad water surface area. Therefore, nighttime relative humidity is raised, and annual foggy days are increased more than 7days and sunshine hours are decreased.

레이저 스페클간섭법에 있어서 스페클크기와 측정 한계에 관한 연구

윤성운,김경석,양승필,정현철,김정호,이도윤 한국공작기계학회 1996 한국생산제조학회지 Vol.5 No.1

The high coherence of laser beam has made it possible to observe interference effects even in the light scattered from rough surfaces. That's why, when object with a scattering surface is illuminated with laser light, we do see a speckled appearance due to random interference. This sort of unique property of laser speckle has brought into existence the new noncontacting techniques such as speckle metrology method of measuring deformation, displacement, and vibration etc of objects with high optical sensitivity. The measurable range of speckle metrology especially used to measure in-plane information, however, is limited by some factors, the so-called strain, rotation, tilt of surface and out of displacement perpendicular to the plane of analysis. This restrictions severly limits the measurable range of speckle metrology by causing the decorrelation of speckle patterns. It is the purpose of this paper to give a survey on the measurable limitation of speckle photography method that is one of speckle metrology. Namely we will discuss the mutual relationships and problems of each limitations adding the restriction of the largest and smallest displacement measurable with speckle methods.

티타늄 레이저 용접부의 기계적 성질 평가

이중재,김길수,안승근,배태성,박찬운 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.2

This study was performed to examine the effects of laser output energy on the mechanical properties of laser-welded titanium joints. Titanium rods of 3 ㎜ in diameter were perpendicularly cut to 30 ㎜ in length for the laser welding. Two rods were fixed to each other with a jig and welded vertically using a Nd:YAG laser at output current of 210-270 A. The penetration depth of the laser beam against the titanium was measured with the changes of output current, pulse duration, and spot diameter. Mechanical tests included tensile testing and Vickers microhardness of the joints. Tensile testing was performed at the crosshead 00speed of 1.0 ㎜/min and a gauge length of 30 ㎜ with a universal testing machine. Vickers microhardness was mesured under 9.8 N load for 15 seconds. The results obtained were summarized as follows; With increasing the output current, the penetration depth of the laser to titanium was increased. Tensile strength values of laser-welded groups were the relatively higher than that of non-welded parent material group. There were significant differences in tensile strength between the specimens of laser-welded at output currents 250 A, 270 A and non-welded parent material group(p<0.05). Microhardness values were increased in the heat-affected zone. Brittle fracture regions were observed in the specimens of laser-welded at output currents 270 A.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.