Anti‑inflammatory effect of Rosa laevigata extract on in vitro and in vivo model of allergic asthma via the suppression of IgE and related cytokines

Seung‑Hyeon Lee,Seung‑Han Choi,In‑Seung Lee,Yumi Kim,Eun‑Jin An,Hyeung‑Jin Jang 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.2

Backgrounds Exposure to a toxic stress environment leads to excessive inflammatory reactions and induces allergic asthma resulting in airway hyper-responsiveness. We investigated whether Rosa laevigata Michx. (RL) exhibits anti-inflammatory effects related allergic asthma in both an in vitro and in vivo studies. Methods To investigate the preventive effect of RL, A549 cells were pretreated with non-toxic doses of RL (500, 1000 μg/ mL) and induced by epidermal growth factor (EGF) (10 ng/mL). First, we evaluated cytotoxicity via a MTT assay. The inhibitory effects of NF-κB activity and COX-2 expression were confirmed by a western blot assay. In the in vivo study, BALB/c mice were challenged with regard to ovalbumin via an intraperitoneal injection of RL (50, 100 mg/kg) and were killed to collect bronchoalveolar lavage fluid, lung tissues and blood serum. The number of inflammatory cells, the secretion of IgE and related cytokines were monitored by ELISA and multiplex assays. Results RL significantly suppressed NF-κB activity and COX-2 expression levels in EGF-induced A549 cells. In a chronic inflammation mice model, pretreatment of RL attenuated allergic airway inflammation by reducing inflammatory cells, the secretion of IgE and related cytokines in a dose-dependent manner. Conclusions The results of this study present the possibility of RL as a therapeutic agent for allergic asthma patients via the suppression of IgE and related cytokines.

원발성 폐암 환자의 생존율에 관한 보고

김현태,이상무,어수택,박춘식,정성환,허승재,남충희,강창희,김용훈 순천향대학교 1994 논문집 Vol.17 No.4

We analysed 404 patients with primary lung carcinoma who were treated at Soonchunhyang University Hospital from July, 1985 to september, 1993 in order to investigate the survival rate and epidemiolgical properties of primary lung cancer. They were 330 males and 74 females. The most prevalent decade was seventh. In terms of cell type, the squamous cell was 225 patients (55%), and adenocarcinoma, small cell, mixed type was 21%, 19%, 4%, respectively. Among non-small cell lung carcinoma, stage Ⅲa was the most prevalent one(92%). In case of small cell carcinoma, the limited stage was 64%. The 12-, 24-, 36- month survival rate of total patients was 57%, 31%, 22%, respectivley and median sruvival time was 15 months. The 36-month survival rate tended to be longer in non-small cell lung carcinoma than that of small cell lung cancer, but there was no difference between two groups, statistically. In non-small cell carcinoma, The 36-month survival rate and meidan survival time were longer in the stage Ⅰ and Ⅱ than those of Ⅲa, Ⅲb, Ⅳ (80% versus 38%, 22%, 0%, p<0.05). According to involvement of lymph node, the 36-month survival rate was longer in NO and N1 than those of N2, N3 (61.9%, 48.7% versus 17.7%, 17.3%, p<0.05). In small cell carcinoma, The 36-month survival rate and median survival rate were higher and longer in limited stage than those of extensive stage(16.1% and 13 month vs 10% and 8 month, p<0.05). In conclusion, we report here the incidence of primary lung carcinoma and the survival rate of paients with primary lung carcinoma who were treated in Soonchunhyang University Hospital.

백서 두개관세포에서 Ipriflavone이 골조직 개조에 미치는 영향

이용승,김영준,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.4

본 연구는 ipriflavone (isopropoxyisoflavone)의 투여가 백서 두개관세포의 증식과 골조직 개조에 미치는 영향을 알아보고자 시도되었다. 태령 20-21일째의 백서 두개관세포를 분리 배양한 후, 10^(-9) M부터 10^(-5) M까지 농도의 ipriflavone을 투여하고 1일째와 3일째에 MTT 분석을 시행하여 흡광도를 평가한 결과, 모든 농도에서 백서 두개관세포의 증식을 보이지 않았다. 한편 골조직 개조에 미치는 영향을 알아보기 위하여 14일째에 alizarin red염색을 시행하여, 형성된 석회화 결절 면적을 측정하였을 때, 10^(-8) M, 10^(-7) M, 10^(-6) M 농도를 투여한 경우 석회화 결절 형성이 유의하게 증가하였다. 골아세포의 분화에 미치는 영향을 알아보기 위하여 ipriflavone을 투여하고 7일째와 14일째에 추출한 RNA를 역전사 중합효소 연쇄반응(RT-PCR)을 시켜 bone sialoprotein (BSP), type 1 collagen (COL I), osteocalcin (OCN) 유전자 발현을 관찰한 결과 BSP와 COL I 유전자는 배양 7일째 높은 발현을 보였고, OCN 유전자는 배양 14일째 높은 발현을 보였다. 이상의 연구결과 ipriflavone이 백서 두개관세포에서 석회화를 촉진시키고 골아세포의 분화에 관여하는 BSP, COL I 및 OCN 유전자 발현을 증가시켜 골조직의 개조를 빠르게 할 수 있음을 시사하였다. lpriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone diazein, has been shown to inhibit bone resorption and perhaps stimulate bone formation. This study was performed to examine the effects of ipriflavone on the proliferation and bone remodeling in rat calvarial cells in vitro. The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured in BGJb media. The graded concentration of ipriflavone (10^(-9)-10^(-5) M) was administered into cultured cells. When the cell proliferation was estimated through the measurement of MTT assay, there was no increase in cellular proliferation of the rat calvarial cell at any ipriflavone concentration. The cellular activity was evaluated through the formation of mineralized nodules stained by alizarin red. The formation of mineralized nodules significantly increased at concentrations of 10^(-8) M, 10^(-7) M and 10^(-6) M ipriflavone. Reverse transcription-polymerase chain reaction analyses (RT-PCR) were done at 7 and 14 days after culture to detect the expression of Bone Sialoprotein (BSP), Type I collagen (COL I) and Osteocalcin (OCN). As a result, the expressions of BSP and COL I increased of the 7th day of culture and the expression of OCN increased on the 14th day of culture. These results indicate that ipriflavone facilitates the bone remodeling process by promoting rat calvarial cell differentiation and stimulating mineralization through increased expression of extracellular matrix genes, such as BSP, COL I and OCN.

항진균물질을 생산하는 Streptomyces fimbriatus AF21-3의 분리 및 동정

장주현,김정완,이우윤,윤영준,이경림,김승곤,이태수 인천대학교 2000 基礎科學論文集 Vol.11 No.1

Actinomycetes were isolated from soil to find new antibiotics. An active antimicrobial compound was isolated from the culture filtrate of Actinomycetes AF 21-3. The Actinomycetes strain which showed strong antifungal activity against Candida tropicalis and Microsporum gypseum was identified as Streptomyces fimbriatus and named as Streptomyces fimbriatus AF 21-3.

8주간의 수영훈련이 호흡순환기능에 미치는 영향

유승희,김복현,심성섭,박찬호 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

In this study, ten students of department of physical education in K unversity participated in 8-week-swimming training. With the figures of respiration and circulation shown before the training and after the trainging, the conclusion came out as follows after comparison and analysis of the changes in the stability and the maximum figures. 1)The respiration of one time ventilation(TVE, ㎖) Looking info the quantity of one lime ventilation In the rest, it came out 946.47㎖, meanwhile, after the training, it fell down to 878.17㎖, which is lower by 68.39㎖ and the the percentage of decrease is 7.78%. But as for the maximum figures, in came out 3117.70㎖, while after the training, it was 3219.10㎖ and that's higher by 101.4㎖ and the percentage of increase is 3.15%. 2)The oxygen uptake(VO2, ㎖/min) In the rest, the quantity of Q2 taken into the lung was 540.83m7/min before the training, but after the trainging, it showed 405.63㎖/min, which was lower by 135.20㎖/min, and the percentage of decrease is 33.33%. But as for the maximum figures, it was 3701.60㎖/min before the training, while after the training, it was 3885.30㎖/min, which Is higher by 183.7㎖/min and the percentage of· increase is 4.73%. 3)The rate of gas exchange(R)In the rest, the rate of gas exchange showed no big difference between before (1.03) and after (1.03) the training. But in the maximum figures, it was 1.42 before the training, while it was 1.45, which was higher by 0.03 and the rate of increase is 2.07%. 4)The oxygen uptake per weight(VO2/W, ㎖/kg/min)In the rest, the quantity of O2 per weight was 8.05㎖/kg/min before the training. But after the training, it was 5.99㎖/kg/min, which was lower by 2.06㎖/kg/min, and the rate of decrease is 34.39%. But in the maximum figures, it was 53.64㎖/kg/min before the trainging, while it was 58.80㎖/kg/min, which was higher by 5.16 and the rate of increase is 8.78%. 5)Heart rate(HR, beats/min)Looking Into the heart rate in the rest, it was 74.24beats/min before the training, but after the training, it was 70.84beats/min, which was lower by 3.4beats/min and the rate of decrease is 4.79%. But in the maximum figures, before the training, it was 187.25beats/min while it came out 194.69beats/min, which was higher by 7.44beats/min and the rate increase was 3.82%. 6)O2 pluse(VO2/HR, ㎖/beats)In the rest figures came out 6.10 before the training, while after the training, it was 5.72㎖/beats which was lower by 0.38 and the rate of decrease was 6.64%. But in the maximum figures, it was 19.78㎖/beats before the training, but after the training, while after the training, it was 22.54me/beats, which was higher by 2.76 and rate of increase is 12.24%.

극장 관람료의 합리적 분배에 관한 연구

이현승,맹수현 영상예술학회 2009 영상예술연구 Vol.15 No.-

부율이란 부금비율의 약칭으로 극장에서 거두어들인 입장수입에서 세금을 제한 후, 배급사와 극장 측이 약정에 따라 수입을 분배하는 비율을 의미한다. 국내에서는 통상 서울 지역의 경우 한국영화는 5:5, 외국영화의 경우 6:4(배급사:극장)로 배분하고 있으며, 지방의 경우 한국 영화와 외국영화의 구분없이 5:5의 배분을 하고 있다. 이는 1980년대 후반 할리우드 영화의 직접배급이 시작되면서 할리우드 영화에 비해 한국 영화의 경쟁력이 취약하여 생긴 관행이다. 하지만 한국 영화의 점유율이 15~30%를 유지했던 8·90년대와는 달리, 평균 50~60%의 점유율을 기록하고 있는 2000년대 이후까지 과거의 관행이 유지되고 있다는 것에는 문제가 있다. 또한 2002년부터 한국 영화의 투자 수익률이 하락하고, 투자, 제작, 배급, 홍보 및 마케팅 등 1차 상영관련 시장의 수익률이 적게는 10%에서 많게는 60% 이상의 마이너스 행진을 기록하고 있는 상황 속에서, 극장 상영부분만이 유일하게 이익을 보고 있다는 것은 산업내 왜곡된 수익구조가 존재한다는 의미이기도 하다. 미국이나 일본, 프랑스에서 극장의 관람료 수익 분배 비율은 10~50% 정도이다. 이를 한국 부율 관행과 비교한다면 그리 나쁘지 않은 수익 분배라고 생각할 수 있으나 사실은 그렇지 않다. 세계적으로 윈도우별 영화시장의 규모 중 극장 매출은 31%이다. 즉 극장 이외의 다른 수익 창출 구조가 안정적으로 유지되고 있다는 뜻이다. 하지만 한국의 경우 부가판권 시장의 몰락으로 투자자에게 돌아가는 수익 중 극장 매출이 차지하는 비율은 전체 매출의 약 80%이다. 그렇기 때문에 극장 관람 수입의 합리적인 배분은 그 어느 나라보다 절실한 실정이다. 영화 산업 내 각 분야의 주체들에게 합리적으로 수익이 분배되기 위해서는 다음의 네 가지 사안들이 고려되어야 한다. 첫째, 제작비, 작품의 가치, 흥행 예상 기대치, 마케팅 규모 등을 두로 고려하여 각각의 작품들은 개별적인 부율 책정에 들어가야 한다. 둘째, 미국의 슬라이딩 시스템이나 프랑스의 ‘월요일 협상(au lundi)’, ‘극장의 ‘통로’(Les ‘couloirs’ de salles)’ 방식과 같이 기간에 따라 차별적인 부율을 적용해야 할 것이다. 셋째, 한국 영화산업의 특수성을 고려한 부율 조정이 필요하다. 넷째, 공정한 거래 절차를 유지하여 바람직한 계약 관행을 확립해야 할 것이다. 한국 영화 산업 내에서 부율 책정은 그동안의 오랜 관행으로 각각의 이익집단간의 이해가 상충하여 쉽게 개선이 될 수 있는 문제는 아닐 것이다. 그러나 이는 한국 영화의 투자·제작 기반을 튼튼히 하여 극장에서도 지속적으로 양질의 콘텐츠를 수급할 수 있도록 하기 위함이며, 나아가 각 사업 분야 모두 합리적인 이익 창출을 통한 안정적인 수익 구조를 실현하여 영화 산업 전반의 성장을 구현하기 위함이다. 또한 합리적 부율 책정을 통해 한국 영화계의 과제인 다양성 영화의 안정적 제작·배급의 풍토 역시 마련될 수 있을 것임을 기대해본다. Box-office split stands for the rate of distribution of box-office earnings between a distributor and a movie theater according to an agreement between them after deducing tax from the earnings. In Seoul, box-office split is usually 5:5 (distributor:movie theater) for domestic movies and 6:4 for foreign movies. In domestic local areas, it is 5:5 in general, regardless of the origins of movies. The difference of box-office split between domestic and overseas movies has been derived from the lower competitiveness of domestic movies than Hollywood movies since Hollywood movies were directly distributed in the late days of the 1980s. The market share of domestic movies was only 15~30% in the 1980s and 1990s, but it has changed to 50~60% since the 2000s. In this vein, still maintaining the past practice is problematic. Besides, since 2002, the investment returns of domestic movies have decreased gradually and the earning rates of primary screening-related market activities, such as investment, production, distribution, publicity and marketing, has been reduced in the rate of minimal 10% to maximal 60%. However, there still exist profits in the part of movie theaters. It means that there is a distorted earning structure in the domestic film industry. The rate of distribution of box-office earnings in the United States, Japan and France is 10~50%. Comparing to the domestic rate, it seems to be not bad. However, it is not the case in reality. In the world, the sales amount of movie theaters occupies only 31% of total earnings which come from all the sales windows of movie market. This means that in overseas film industry there are stable earning structures other than movie theaters. However, with the destruction of domestic additional copyright market, the sales amount of movie theaters occupies 80% of investors' total income in the domestic film industry. Therefore, it is urgent to rationally distribute the box-office earnings of domestic movie theaters. In order for the box-office earnings of domestic movie theaters to be rationally distributed among all the parties of film industry, following four alternatives must be taken into account:First, each movie must have its own box-office split considering production cost, the value of movie, expected box-office value, marketing scale and so on;second, like American sliding system or French ‘au lundi’ and ‘Les couloirs de salles’ systems, differentiated distribution rates must be applied on the basis of the period; third, we need an adjustment considering Korean films industry's speciality; and fourth, fair transaction procedures and proper contract practices must be established. Since the existing box-office split is a practice of long standing and the interest of every party is contrary, the establishment and improvement of rational box-office split in domestic film industry seems to be not easy. However, to establish a rational box-office split is necessary for strengthening the investment and production base of domestic movies and supplying quality movies to movie theaters. In addition, it is necessary to establish a rational investment return-based stable earning structure for all the related fields of film industry and thus realize the growth of the film industry in general. The investigator hopes that with the establishment of rational distribution rate, the environment of stable production and distribution of diverse movies, a task of long standing in the domestic film industry, will be created in the future.

自然과 調和를 이룬 光山區의 文化觀光資源 開發 推進方向

김승현 조선대학교 지역사회발전연구원 2003 地域發展硏究 Vol.8 No.1

Gwangsan-gu is called the hidden place for Gwangju with its beautiful scenery. We must develop this inherited natural resources for the rest place of residents and the cultural sightseeing points with pro-environmental plans. Especially, Mt Udoong. Mt youngjun and Hwangyong river must be developed for the rest place of domestic and foreign tourists, preservation of the local history and the cultural resources. It will contribute to the enhancement of the life quality of residents and the personal income. By doing so, Gwangsan will take a firm root as a central city in the Korean South-west zone, coexisting cities and rural districts.

고유량 고효율 혈액투석막 재사용의 임상경험

조현경,김승정,임병국,정혁준,최영일,마경애,신규태,김흥수,김도헌 대한신장학회 2001 대한신장학회잡지 Vol.20 No.3

Budd-Chiari 증후군 : 1례 보고 A Case Report

김승현,오연희,이현경,이성우,서정욱 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

하대정맥의 막성폐쇄로 인한 Budd-Chiari 증후군은 비교적 드문 질환으로 알려져 있다. 저자들은 우상복부 동통을 주소로 내원한 환자에게 방사선학적 검사와 임상적으로 확진된 하대정맥의 막성폐쇄에 의한 Budd-Chiari 증후군 1례를 경험하였기에 문헌고찰과 함께 보고 하고자한다. The Budd-Chiari syndrome is a rare, often fatal illness resulting from hepatic venous outflow occlusion. It occurs secondary to intrinsic vascular thrombosis, tumor invasion, or rarely a congenital web. We report a case of primary Budd-Chiari syndrome with membranous obstruction of inferior vena cava, with a brief review of literature.

플라이오메트릭 트레이닝이 조정 에르고메타 기록에 미치는 영향

육현철,이성윤,이기철 한국체육대학교 체육과학연구소 2000 韓國體育大學校附屬 體育科學硏究所論文集 Vol.19 No.1

The purpose of this study was to investigate the effects of plyometric training in 2000m rowing egrometer race. 8 male subject were high school rowing player in Inchun. They were randomly assigned to two groups. subjects training for six week performing plyometric training. The test item included 1) total 2000m record 2) each 500m record 3) heart rate 4) each 500m watt. The results of the study from the plyometric training are as follows : 1.The results of each 500m record were not significant difference (p).05) 2.The results of each 500 Watt record were not significant difference (p).05) 3.The results of each minutes heart rate were not significant difference (p).05) base on above results, it do not showed the differences in plyomeric training. But act practical rowing race, victory and defeat is showed 1~2sec difference. So, reduce 3~4sec record, it has an effect.