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정혁준 한국형사판례연구회 2009 刑事判例硏究 Vol.17 No.-
Despite of common opinion that aiding or abetting of false accusation against oneself can not establish a crime, Supreme court adjudged a person who aided false accusation against himself to be guilty recently. With the help of this judgement of conviction and a Practical need of punishing such a crime, prosecution and punishment on a person who commits a this kind of crime would increase rapidly. So in this brief report let us survey how variously this crime can be committed and to what extent it can be constituted. Also we will consider a crime of ‘interference with government official in the execution of his duty' as a substitute.
고정화 Corynebacterium pseudodiphtheriticum W3712에 의한 화학유연제, Organopolysiloxane의 제거
정혁준,이정훈,김정,김현수,유대식,Jung, Hyuck-Jun,Lee, Jung-Hun,Kim, Jung,Kim, Hyun-Soo,Yu, Tae-Shick 한국미생물학회 2000 미생물학회지 Vol.36 No.2
화학유연재, organopolysiloxan분해세균인 Corynebacterium pseudiptheriticum W3712의 생물학적 활성을 증가시키기 위하여 균체를 고정하시켰다. 이 세균의 고정화 담체로서는 bentonite가 가장 양호했으며, bentonite에 의한 organopolusiloxane의 물리적 흡착효율은 62.5%였다. 실험균주의 배양액 16ml를 bentonite 20g에 고정시킴 고정화균체 10.0g은 12,000 mg/l의 organopolysiloxaneqnsgotprbs 폐수를 실온에서 10일간 정치배양하므로 95.0% 제거시켰다. 이상의 결과로 이 고정화 균체를 공장의 폐수 저장탱크를 개조하지 않고 정화할수 있다고 사료되었다. In order to increase biological activities of Co~nebacteriumpseudodipI~t~~eriticum W3712 which degrades achemical softener (organopolysiloxane), the cells were immobilized on four immobilizing carriers by physicaladsorption. The cells immobilized on bentonite had higher removal efficiency than those immobilized onthe other carriers, and physical adsorption of organopolysiloxane on bentonite was 62.5%. The immobilizedcells were produced by 16 ml of culture broth with 20 g of bentonite, and 100 ml of the standard wastewatercontaining 12.000 mgll COD," with organopolysiloxane was removed 95.0% by 10.0 g of the immobilizedcells treatment at room temperature for 10 days by static culture.y static culture.
Monascus 속 균주의 균체 생산 및 고체배양에 의한 Monacolin K 생산
정혁준,유대식 한국미생물학회 2004 미생물학회지 Vol.40 No.2
The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$PO$_4$, 0.05% The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % $(KH_2PO_4$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.2% L-asparagine, pH 4.5, and the optimal inoculum size and shaking speed were $1.5{\times}10^6$ spores/50 m1 medium and 150 rpm, respectively. On optimal conditions, 4.1 g/l of the cell mass was obtained at 28$^{\circ}C$ for 3 days. The mycelium were inoculated on 500 g of steamed rice using vinyl bag ($30.6{\times}44$ cm) and incubated at $30^{\circ}C$, 85% humidity for 21 days. Lactone form monacolin K was rapidly increased for 2 days and reached highest concentration of monacolin K (2,930 mg/kg) for 15 days, and monacolin K was decreased after 15 days.
정혁준,한상덕,강명주 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.9
Solid dispersion (SD) systems have been widely used to increase the dissolution rate and oral absorption of poorly water-soluble compounds. In order to enhance the dissolution rate of dronedarone hydrochloride (DRN), a recent antiarrhythmic agent, SDs of DRN were formulated using conventional solvent evaporation method with amorphous polymers including hydroxypropyl methyl cellulose (HPMC), poly(vinyl pyrrolidone) (PVP), and vinylpyrrolidone-vinyl acetate copolymer (VA64). The prepared SDs were characterized in terms of drug crystallinity, morphology, and in vitro dissolution profile in aqueous medium. The physical characterization using differential scanning calorimetry and X-ray powder diffraction revealed that the active compound was molecularly dispersed in all polymeric carriers tested, in a stable amorphous form in drug to polymer ratios ranging from 1:0.5 to 1:2. The dissolution rates of DRN in all SDs were much higher than those from the corresponding physical mixture and drug powder alone. In particular, the greatest dissolution enhancement was obtained from the VA64-based SD in a drug to polymer weight ratio of 1:1, achieving almost complete drug release after 120 min at pH 1.2. Thus, VA64-based SD with higher drug dissolution rate along with a simple preparation process is suggested as an alternative for the oral formulation of the benzofuran derivative.
Aspergillus coreanus NR 15-1과 Aspergillus oryzae NR 2-5의 원형질체 형성의 최적조건
정혁준,유대식 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.1
한국 전통누룩으로부터 분리·동정된 Aspergillus coreanus NR 15-1로부터 glucoamylase활성이 높은 X11(Arginine^-) 변이주와 A. oryzae NR 2-5로부터 α-amylase활성이 높은 Z6 (Adenine^-) 변이주의 원형질체융합을 위한 원형질체 형성의 최적조건을 검토했다. 원형질체 형성을 위한 세포벽 분해효소는 X11 변이주와 Z6 변이주 모두 cellulase와 driselase를 복합적으로 각각 10mg/ml되게 사용하였을 때 원형질체 형성율이 가장 높았다. 삼투압 안정체는 X11 변이주에서는 0.4 M NaCl이, Z6 변이주에서는 0.6 M NaCl이 가장 효율적이었다 원형질체 형성을 위한 반응 최적 pH는 X11 변이주와 Z6 변이주 모두 pH 5.0에서 가장 높은 원형질체 형성율을 나타내었으며 pH 6.0에서는 형성율이 반 이하로 감소하였다. 반응 최적온도는 두 변이주 모두 30℃가 가장 적합하였으며 그 이상의 온도에서는 원형질체 형성율이 감소하였다. 원형질체 형성을 위한 세포벽 분해효소의 반응 최적시간은 X11 변이주에서는 반응 5시간에, Z6 변이주에서는 반응 6시간이 원형질체 형성율이 가장 높았다. 원형질체의 재생율에 있어서는 두 변이주 모두 반응 4시간 때 X11 변이주가 11.3%로, Z6 변이주는 11.6%로 가장 높았으며 재생되는 원형질체의 수도 가장 많아 반응 시간은 4시간이 적당한 것으로 나타났다. 이상의 조건에서 형성된 원형질체의 평균 크기는 X11 변이주가 3~6㎛ 이며, Z6 변이주는 4~9㎛ 로 다양한 크기의 원형질체가 형성되었으며 원형질체 형성율은 각각 6.54 × 10 exp (7) protoplasts/ml와 3.04 × 10 exp (7) protoplasts/ml이었다. Aspergillus coreanus NR 15-1 and Aspergillus oryzae NR 2-5 from traditional Korean Nuruk were selected as parental strains producing starch hydrolysis enzymes. X11(Arginine^-) mutant from A. coreanus NR 15-1 showed high glucoamylase activity and total acid productivity. Z6(Adenine^-) mutant from A. oryzae NR 2-5 showed the highest α-amylase activity. Therefore, both X11 and Z6 mutants were selected and investigated for the optimal conditions of protoplast formation for protoplast fusion. Mixture of equal amount of cellulase and driselase(10 mg/ml each) was the most effective as lytic enzymes. The optimal pH and temperature for protoplast formation were 5.0 and 30℃, respectively. The most effective reaction for protoplast formation time was 4 hours. The maximum of protoplast formation of X11 mutant and Z6 mutant were 6.54 × 10 exp (7) protoplasts/ml and 3.04 × 10 exp (7) protoplast/ml, and the regeneration frequencies of the protoplasts were 11.3% and 11.6%, respectively. The size of the protoplasts from X11 and Z6 mutants were 3~6 ㎛ and 4~9 ㎛, respectively.