Streptomyces sp. Y9343이 生産하는 齒面細菌膜 分解酵素의 精製와 特性

김성주,한홍근,윤정원 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

치면세균막을 제거하거나 형성을 억제하여 치아우식증 예방제를 개발할 목적으로 α-1,3 glucanase를 분비하는 새로운 균주로 부터 분리 정제하여 그 특성을 조사하였다. 불용성 glucan을 유일탄소원으로 하는 agar plate를 제조하여 토양으로 부터 α-1,3 glucanase 분비균주를 탐색한 결과 Streptomyces sp. Y9343을 얻었다. 액체배양시 최적 효소생산조건은 탄소원으로 1% soluble starch와 indcer로 0.5% insoluble glucan을 첨가하였을 때 가장 효율적이었다. α-1,3 glucanase는 황산 암모늄 염석, DEAE-CEllulose 이온교환 크로마토그래피, Sephadex G-75 겔 여과 등에 의하여 32.1배까지 정제되었고 수율은 0.53%이었으며, 이 때의 활성도는 7840.9 U/mg protein이었다. 정제된 α-1,3 glucanase를 SDS-PAGE로 분석한 결과, 단일체임을 확인하였으며, 이 때 분자량은 22,500이었다. 효소의 최적 pH는 6.5이었다. 효소의 최저온도는 37℃이었고, 열에 대한 안정성은 70℃ 이상에서 40%~60%의 효소활성이 상실함을 보였다. Detergent의 영향은 SDS에 의해 83%, Tween 20에 의해서는 약 27% 정도의 활성저해를 받았다. 효소활성의 금속이온에 의한 영향은 Co^2+, Mn^2_+에 의해 각각 81.8, 69.7%의 활성의 증가를 보였고 이들의 최적농도는 10mM이었으며, 반면에 Hg^2+에 의해서는 93.9%의 효소활성의 저해를 나타내었다. 또한 초기속도(30분 이내)에 금속이온에 의한 영향이 큰 것으로 나타났다. α-1,3 glucanase의 불용성 glucan에 대한 K_m 값은 2.50mM이었고, V_max는 0.0431 mM/min이었다. α-1,3 glucanase의 기질특이성을 조사한 결과, 반응 30분 후 IG와 soluble starch에는 각각73, 100%의 높은 분해력을 보였으며, raw starch, dextran T-10에 대해서는 낮은 분해력을 보였다. 한편, 인조치면세균막을 S. mutans로부터 시험관 벽에 제조한 후, α-1,3 glucanase를 처리한 결과 2시간 이내에 완전히 분해 제거되는 것을 알 수 있어 강력한 치아우식예방제로 개발될 수 있음을 보여 주었다. Streptococcus mutans has been implicated as primary causative agents of dental caries by insoluble glucan (IG) in human and experimental animals. An attempt was made to search for the α-1,3 glucanase that degrades IG produced by S. mutans. α-1,3 glucanase was detected in the culture supernatant of microorganisms, which are isolated from soils on agar medium containing IG as a sole carbon source. This Streptomyces sp. hydrolysed IG produced by immobilized S. mutans and was named as Y9343. This enzyme required α-1,3 glucan (IG) as an inducer. The optimum conditions for enzyme production were studied. The enzyme was purified by 30~70% (NH_4)_2SO_4 precipitation, anion exchange chromatography on DEAE-cellulose and gel filration on Sepadex G-75. The purified enzyme has a specific activity of 7840.0 U/mg protein giving 32.1-fold purification and final yield of 0.53%. The molecular weight was estimated to be about 22.5 kDa by SDS-PAGE. The optimum pH and temperature for enzyme reactiorr were 6.5 and 37℃, respectively and the enzyme was relatively stable at the temperature below 60℃. The activity of purified enzyme was enhanced by adding Co^2+, Mn^2+, and Mg^2+ into the medium, whereas inhiited by adding Hg^2+, Zn^2+ and SDS. The K_m and V_max value of α-1,3 glucanase for IG were estimated to be 2.50 mM and 0.0431 mM/min, respectively. The thin layer chromatographic analysis of hydrolysates from IG with α-1,3 glucanase showed that glucose was the main product of reaction. This enzyme activity was about 14 times higher than marketing dextranase as preventive agent against artificial dental caries by S. mutans in TH medium including 5% sucrose after 30 minutes.

기체온도 변화에 따른 질소레이저의 출력특성

김성훈,양준묵,추한태,이치원 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.2

While the temperature of the gas was being changed by modifying the value of E/P which being effects the output characteristics of gas laser into a temperature function, the optimum value of E/P was calculated, being applied to Nitrogen laser and was compared with experimental values. The optimum operational characteristics by the variation of the nitrogen gas temperature is observed at 30 ℃ in gas temperature and 120 torr in gas pressure. Also the value of E/P is evaluated to be 423 V ㎝^-1torr^-1 which is 2.2 times as large as that of theoretical value. According to increasing the nitrogen gas temperature, the nitrogen gas pressure for the laser action is to be broaded. However in this case, the maximum output power is slightly decrease.

벼 밭재배에 따른 수량 및 미질

최원영,최민규,김상수,이규성,김태수,이재길 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

벼 밭재배시 논벼 품종들의 적응성을 검토하기 위하여 마늘 간작으로 벼를 건답점파하여 재배한 결과를 요약하면 다음과 같다. 1. 입모율은 밭벼에 비해 논벼 품종이 낮았는데 그 순서는 상남밭벼〉동진찰벼〉신선찰벼〉진부찰벼 순이었다. 2. 출수기는 상남밭벼에 비해 진부찰벼는 3일 빨랐고 신선찰벼는 9일, 동진찰벼는 12일이 늦었다. 3. ㎡당 수수와 등숙비율은 밭벼에 비해 논벼품종들이 높았다. 4. 쌀수량은 상남밭벼에 비해 신선찰벼는 12%가 많았고 동진찰벼는 같았으며, 진부찰벼는 18%가 적었다. 5. 완전립비율은 진부찰벼에서 높았고 그 외는 비슷하였으며, 식미지수는 상남밭벼에 비해 동진찰벼는 높았으나 진주찰벼와 신선찰벼는 낮았다. To identify the rice varieties adaptable for upland cultivation, a series of experiments was carried out at the field(Chonnam series) of the Muan. Cheonnam province during 1999 - 2001. Four varieties, Jinbuchalbyeo(early-maturing variety), Sinseonchalbyeo (medium-maturing variety), Dongjinchalbyeo(medium-late maturing variety), and Sangnambatbyeo as a standard variety for upland were used in this experiment. Seedling establishment of the varieties was the highest in Sangnambatbyeo and followed by Dongjinchalbyeo under upland condition. Heading date in general delayed ranges from 9 to 12 days, while early-maturing variety, Jinbuchalbyeo was faster for 3 days compared with Sangnambatbyeo. Number of panicle per square meter was similar in both lowland and upland rice but percentage of ripened grain was high in lowland rice. Milled rice yield was high as of 12% in Sinseonchalbyeo compared with Sangnambatbyeo. Head rice ratio showed also the highest in Sinseonchalbyeo. The results indicate that Sinseonchalbyeo as glutinous rice variety could adapt well in this upland condition, and have good milling recovery as head rice as well as yield advantage.

간척지 중염답에서 유수형성기 염처리에 따른 벼 생육 및 수량 변화

최원영,이규성,고종철,김상수,김태수 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

본 연구는 새계화벼를 공시하여 간척지 중염답(0.3~0.4%)에서 관개수 염농도에 따른 생육 및 수량성을 검토하고자 유수형성기에 5일간 염수를 처리하여 시험한 결과를 요약하면 다음과 같다. 1. 관개수 염분농도가 높을수록 벼 출수는 1~2일 지연되었고, 출수기 지상부건물중은 가벼워졌다. 2. 간장과 수장은 관개수 염분농도가 높을수록 짧아졌다. 3. 관개수 염분농도가 높을수록 수당 립수가 적어 ㎡당 립수가 적었으며, 등숙비율이 낮고 현미 천립중이 가벼워, 쌀 수량은 민물 관개(무처리)의 330㎏/10a에 비해 관개수 염분농도 0.1%는 94%, 0.3%는 85%, 0.5%는 76%, 0.7%는 71%의 수량을 얻었다. 4. 관개수 염분농도가 높을수록 완전립 비율은 낮아졌다. 따라서 간척지에서 중염도 토양(0.3~0.4%)에서 벼를 재배하여 유수형성기에 한발이 닥쳐 저류지 등 염분 농도 0.7%의 물로 5일간 담수하여도 쌀 수량은 29% 정도 감수되고 쌀의 품위도 저하되지만 어느 정도 수량은 얻을 수 있을 것으로 생각된다. This experiment was conducted at the Kyehwado Substation of the National Honam Agricultural Experiment Station(NHAES) RDA, Korea. The experimental field contained 0.38% NaCl in soil solution. The experiment was involved five treatments (control, 0.1, 0.3, 0.5, 0.7%) in a randomized complete block design with three replicates, saline solution was applied only one time at panicle formation stage for 5 days. Saegyehwabyeo, a japonica rice variety was used in this experiment. In yield components, Spikelets number per ㎡ decreased with increasing salinity level, particularly in the 0.7% of saline solution water. This factor affected the most yield reduction among the components. The percentage of ripened grain was inclined to decrease with increasing salinity level. 1,000 grain weight decreased with increasing salinity level but it was lest affected by salinity among yield components. The reduction of milled rice yield decreased significantly with increased saline water level, in detail 6% of yield reduction at the 0.1% saline solution, 15% at the 0.3%, 24% at the 0.5%, and 29% at the 0.7% saline solution level compared with control respectively. Results indicate that the gaining of rice yield could be reliable performance in terms of economical benefit for rice production even though high reduction of yield occurred at high salinity levels on a reclaimed saline soil in Korea.

간척지 벼 담수직파재배시 토양 염농도별 적정 최아정도

최원영,이규성,김정곤 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

간척지에서 벼 담수직파재배시 입모 향상을 위하여 토양 염 농도별 적정 최아 정도를 구명하기 위하여 시험한 결과는 다음과 같다. 가. 입모수는 저염 토양은 건종자 파종에서만 적었고 그 외 처리에서는 비슷하였으며, 중염 토양은 반대로 최아 0㎜와 건종자 파종에서 많았다. 나. 출수기는 저염 토양에서는 최아 4㎜가 8월 18일인데 비하여 2 ㎜와, 0 ㎜에서는 같았으나 소독후 건조종자 파종에서는 1일이 늦었으며. 중염 토양에서는 최아 4㎜에 비해 2㎜는 같았고 건조종자 파송에서는 1일이 늦었으나 0㎜ 파송에서는 오히려 1일이 빨랐다. 다. 저염 토양에서는 ㎡당립수가 최아 4>2>0㎜>건종자 순으로 많아 쌀 수량도 최아2~4 ㎜에서 많았으며, 중염 토양에서는 도복에 강하고 등숙비율이 높은 최아 0㎜와 건 조종자 파송에서 쌀 수량이 많았다. 따라서 저염 토양에서는 최아 2~4㎜가 적당하고, 중염 토양에서는 최아 0㎜ 또는 소독 후 건조종자 파종이 적당할 것으로 판단된다. This study was conducted to examine an appropriate pregermination treatment of rice seeds for wet seeding on the wet surface of a reclaimed saline soil. It was conducted at the Gyehwado Substation of the Honam Agricultural Research Institute in 2002~2003. Two soil saline solutions (0~0.1% NaCl and 0.3~0.4% NaCl) and four pregermination seed treatments (nonsoaking, water absorption only, 2 ㎜ pregerminated, and 4 ㎜ pregerminated) were used. The experiment was carried out in a split plot design with three replications. At low soil salinity (0~0.1% NaCl), the number of spikelets and the percentage of ripened grain increased with a longer sprouting length of rice seed compared with that of nonsoaking (dry seeds) and water absorption only. Pregerminated seed treatments showed significant high rice yield performance in low saline conditions indicating pregerminated treatments are necessary for good seedling establishment and growth of rice. However, rice seeds treated with nonsoaking and water absorption only had higher yield performance in a medium soil salinity level (0.3~0.4%) than the pregerminated ones. This result indicated that pregerminated seeds seeded under a medium soil salinity level could have damaged shoot tips and roots, thus the growth would be retarded and soaking seeds float because of high soil salinity.

라인케 부종의 수술 전후 음성분석

김동영,성원진,황찬호,이상준,고태용,전상준,김광현,성명훈 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.9

급성 인두 및 편도염에서 4-nitro-2-phenoxymethanesulfonanilide(Nimesulid)의 임상효과

유장렬,윤춘원,윤성기,김무명,박찬일 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

Nimesulid, 4-nitro-2-phenoxymethanesulfonanilide, a non-steroidal antiinflammatory drug endowed with potent anti inflammatory, analgesic and antipyretic activities was tested at a daily dose of 200mg b.i.d. against 40 cases of acute pharynogotonsillitis. The therapeutic effect was appeared in 36 cases(90%): 13 cases(32.5%) in Group I, 12 cases(30%) in Group Ⅱ, 11 cases(27.5%) in Group Ⅲ. 2 cases complained gastrointestinal discomfort.

식도를 포함한 전위장관을 침범한 전이성 악성 흑색종 1예

최정영,김준기,신성하,고영춘,조상욱,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.1

국내 정신과 의사의 항정신병 약물 장기 지속형 주사제 처방에 대한 인식조사

박한선,배승민,유재홍,김수인,임원정 大韓神經精神醫學會 2009 신경정신의학 Vol.48 No.3

Objectives Long acting injectable antipsychotic medication have the ability to sustain the drug effect for a long duration, thus enabling advances in the management of those psychiatric patients who have poor compliance or who have difficulty getting the oral form of their antipsychotic medications. Despite their benefits, previous studies have reported that depot antipsychotics have a much lower prescription ratein the treatment of schizophrenia or schizoaffective disorder. Psychiatrist's attitude toward depot antipsychotics clearly has an influence on the decision-making process regarding the selection of medications. Until now, there has been no data with respect to psychiatrists' attitudes regarding long-acting injectable antipsychotics in Korea. This Study examined these attitudes toward antipsychotic depot medications as well as the factors which could contribute to their choice of deports. Methods We questioned 347 psychiatrists, who had been attending a conference, about their attitudes toward depot antipsychotic treatment. Results The most important factor causing reluctance with respect to prescribing depot treatment (for both classes of antipsychotics) was a presumed sufficient level of compliance with available oral antipsychotic treatments. In addition, typical depots are not considered to be an appropriate treatment option for first-episode patients, and as a result, they are used sparingly. Lastly, atypical long-acting injectable drugs are avoided due to their strict criteria of insurance and high treatment costs. Conclusion Aversions to prescribe depot treatment are frequent among psychiatrists and appear to be unrelated to the antipsychotic class. The stated reasons for not choosing depots are generally not supported by the current literature. Further research is required to clarify the advantages using depot treatments.

외상성 하인두-경부 식도 손상

이강진,성명훈,박범정,성원진,노종렬,정산신,이승신,김광현 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.11