Delftia sp. JK-2로부터 분리된 Catechol 2,3-dioxygenase의 정제와 특성 연구

황선영,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The purpose of this work was to investigate the purification and characterization of catechol 2,3-dioxygenase isolated from Delftia sp. JK-2, which could utilize aniline as the sole source of carbon, nitrogen and energy. In initial experiments, several characteristics of C2,3O separated with ammonium sulfate precipitation and DEAE-sepharose were investigated. Specific activity of C2,3O was approximately 4.72 unit/㎎. C2,3O demonstrated its enzyme activity to another substrates including catechol and 4-methylcatechol. The optimum temperature of C2,3O was 30℃, and the optimal pH was approximately 8. Metal ions including Ag^(+), Hg^(+), and Cu^(+2) showed inhibitory effect on the activity of C2,3O. Molecular weight of the enzyme was determined to approximately 35 kDa by SDS-PAGE.

만성정신질환자의 직업재활 사례

황태연,한은선,이영문,강순정,정경선 용인정신의학연구소 1995 용인정신의학보 Vol.2 No.1

활성 슬러진에서 분리한 세균 Delftia sp. JK-2에 의한 산업 유기용매의 제거

황선영,유미옥,오계헌 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. A bacterium which had excellent aniline degradability was isolated for this work. Biodegradation of industrial organic solvents such as aniline, benzoate, or p-hydroxybenzoate by Delftia sp. JK-2 was examined in the media containing the target substrates in Erlenmeyer flasks, respectively. The JK-2 cells degraded 10 mM aniline, 5 mM benzoate or 5 mM p-hydroxybenzoate completely within 36 hours, 48 hours or 40 hours, respectively. Microscopic examination of the strain revealed Gram-negative and short rod cells. Physiological analysis using BIOLOG GN2 MicroPlate™ system was performed to identify the strain JK-2. As the results of this identification processes, the strain JK-2 could be assigned to genus Delftia, and designated as Delftia sp. JK-2

점액성췌장 낭종 1예

황석주,김영운,오성광,김대영,임선재,박승욱,이두용,이헌영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Pancreatic cystadenoma is very rare, slow growing tumor and generally preoperative differential diagnosis may be difficult. A 43-year-old female was admitted to our hospital with the complaint of abdominal discomfort in the epigastrium for about preceeding 5 years. Ultrasonography and Computed tomography were performed. When endoscopic retrograde cholangio-pancreatogram was performed, displacement of distal pancreatic duct by huge mass was noted and injected dye was drained irregulary into the cavity of mass. Distal pancreatectomy was performed with splenectomy. The mass size of distal pancreas was 7X4cm, and the cyst contained with yellowish thick mucoid fluid. Diagnosis was confirmed the mutinous cystadenoma of the pancreas on histologic examination.

일부 응급구조과 학생과 간호과 학생의 안락사에 대한 태도조사 연구

김미선,박미화,황선영 한국응급구조학회 2002 한국응급구조학회지 Vol.6 No.1

This descriptive study was conducted to identify the attitude toward euthanasia of EMT and nursing students and to compare their attitude before and after clinical practice. The convenience sample was comprised of 40 first grade and 40 second grade EMT students attended at G college in G-city, and 40 first grade and 40 third grade nursing students attended at C college in C-province. The variable was tested with an euthanasia attitude scale developed by Kim Ae Kyoung(2001). The valid responses were obtained and analyzed by using SPSS PC+ from November 25 to December 6.2002. The results showed that most of the respondents agreed to have positive attitude toward euthanasia in terms of client's right and client's quality of life. Also, they thought that euthanasia should be legalized and the right to die with dignity should be guaranteed. The EMT respondents having a clinical experience showed significantly higher score than respondents having no experience in the subarea of medical ethics and nursing respondents who had a clinical practice showed significantly higher score than others no experience in the subarea of quality of life. But, the other sub-areas had no significant difference between the respondents before and after clinical practice.

理解點檢方略이 敎材回想에 미치는 效果

文善模,黃永信 경상대학교 중등교육연구소 1994 현대교육연구 Vol.6 No.-

The purpose of this study was to investigate the effects of comprehension monitoring strategy on text recall. The hypotheses to be tested were as follows: (1)There will be a significant difference in recall scores of the idea units between types of comprehension monitoring strategy(main idea. detail idea. control). (2) There will be a significant difference in recall scores of the idea units between levels of verbal ability (high vs low). (3) There will be a significant interaction effect in recall scores of the idea units between types of comprehension monitoring strategy and levels of verbal ability. (4) There will be a significant interaction effect in recall scores of the idea units between types of comprehension monitoring strategy and levels of idea unit(high vs low). Subjects of this study 84 female high school students selected randomly on the basis of levels of verbal ability. And they were randomly assigned to experimental conditions. The stimulus material was expository text about Islram. It was consisted of three paragraphs and about 220 words. Each of the three paragraphs in text focused on a separate aspect of the main topic. The first paragraph dealt with the social class. the second with the economy, and the third with civilization. The middle paragraph was modified to contain inconsistent information at main idea or detail idea. To test the hypotheses. 3(types of comprehension monitoring strategy : main idea. detail idea. control) × 2(levels of verbal ability : high vs low) × (2)(levels of idea unit : high vs low) ANOVA was applied for recall scores of the idea units. The main findings of the study were as follows: (1) There was a significant main effect of types of comprehension monitoring strategy on recall scores of the idea units. Main idea comprehension monitoring strategy and detail idea comprehension monitoring strategy had more facilitative effect than control condition. (2) There was a significant main effect of levels of verbal ability on recall scores of the idea units. That is. higher verbal ability condition had more facilitative effect than lower verbal ability condition. (3) There was no significant interaction effect between types of comprehension monitoring strategy and levels of verbal ability on recall scores of idea units. (4) There was a significant interaction effect between types of comprehension monitoring strategy and levels of idea units on recall scores of the idea units,

성인 자원자에서 B방식 초음파를 이용해 측정한 경동맥 내막중막두께의 분포

이선규,황희영,김형식,장미선,이은주,강문호,고광곤 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

탄성계수 감소곡선에 근거한 철도노반의 회복탄성계수 모델 개발 및 평가

박철수(Park Chul-Soo),황선근(Hwang Seon-Keun),최찬용(Choi Chan-Yong),목영진(Mok Young-Jin) 한국철도학회 2008 한국철도학회 학술발표대회논문집 Vol.- No.-

This study focused on the resilient modulus prediction model, which is the functions of mean effective principal stress and axial strain, for three types of railroad trackbed materials such as crushed stone, weathered soil, and crushed-rock soil mixture. The model is composed with the maximum Young"s modulus and nonlinear values for higher strain in parallel with dynamic shear modulus. The maximum values is modeled by model parameters, A<SUB>E</SUB> and the power of mean effective principal stress, n<SUB>E</SUB>. The nonlinear portion is represented by modified hyperbolic model, with the model parameters of reference strain, εr and curvature coefficient, a. To assess the performance of the prediction models proposed herein, the elastic response of a test trackbed near PyeongTaek, Korea was evaluated using a 3-D nonlinear elastic computer program (GEOTRACK) and compared with measured elastic vertical displacement during the passages of freight and passenger trains. The material types of sub-ballasts are crushed stone and weathered granite soil, respectively. The calculated vertical displacements within the sub-ballasts are within the order of 0.6㎜, and agree well with measured values with the reasonable margin. The prediction models are thus concluded to work properly in the preliminary investigation.

탄성계수 감소곡선에 근거한 철도노반의 회복탄성계수 모델 개발 및 평가

박철수(Park Chul Soo),황선근(Hwang Seon Keun),최찬용(Choi Chan Yong),목영진(Mok Young Jin) 대한토목학회 2009 대한토목학회논문집 C Vol.29 No.2

본 연구에서는 국내 철도 토공노반 재료로 가장 흔히 사용되는 입도조정쇄석, 화강풍화토, 암버럭-토사 혼합 재료에 대해 평균유효주응력과 축변형률의 함수로 표현되는 회복탄성계수 예측모델을 결정하였다. 회복탄성계수 예측모델은 대표적인 동적물성치인 변형률에 따른 전단탄성계수 감소곡선의 표현과 같이 최대영탄성계수와 정규화 영탄성계수 감소곡선으로 구성된다. 평균유효주응력의 함수로 표현되는 최대영탄성계수의 모델인자는 A<SUB>E</SUB>와 n<SUB>E</SUB>이고, 비선형 영역의 정규화 영탄성계수 감소곡선은 기준변형률(ε<SUB>r</SUB>)과 곡률계수(a)를 모델인자로 하는 수정 쌍곡선 모델로 표현된다. 제안된 회복탄성계수 예측모델을 검증하기 위해 3차원 다층탄성해석 프로그램(GEOTRACK)을 이용하여 평택 시험 철도노반의 탄성거동을 평가하였고, 화물열차 및 여객열차가 시험구간을 통과할 때 계측한 노반의 수직 탄성변위와 비교하였다. 현장계측은 자갈도상 아래의 재료가 각각 입도조정쇄석과 양질의 화강풍화토인 두 개소에서 수행되었다. 자갈도상 아래에서 계산된 수직 탄성변위는 대략 0.6㎜ 이내였고 계측 결과와 잘 일치하였다. 본 연구를 통해 제안된 회복탄성계수 예측모델이 열차하중에 의한 노반의 탄성거동을 적절히 표현하고 있음을 확인하였다. This study is to develope the resilient modulus prediction model, which is the function of mean effective principal stress and axial strain, for three types of railroad trackbed materials such as crushed stone, weathered granite soil, and crushed-rock soil mixture. The model consists of the maximum Young's modulus and nonlinear values for higher strain, analogous to dynamic shear modulus. The maximum value is modeled by model parameters, A<SUB>E</SUB> and the power of mean effective principal stress, n<SUB>E</SUB>ㆍThe nonlinear portion is represented by modified hyperbolic model, with the model parameters of reference strain, ε<SUB>r</SUB> and curvature coefficient, a. To assess the performance of the prediction models proposed herein, the elastic response of a test trackbed near PyeongTaek, Korea, was evaluated using a 3-D elastic multilayer computer program (GEOTRACK). The results were compared with measured elastic vertical displacement during the passages of freight and passenger trains at two locations, whose sub-ballasts were crushed stone and weathered granite soil, respectively. The calculated vertical displacements of the sub-ballasts are within the order of 0.6㎜, and agree well with measured values. The prediction models are thus concluded to work properly in the preliminary investigation.

熟地黃의 抱製에 따른 5-hydroxymethyl-2-furaldehyde 함량 연구

이제현,고정아,황은영,홍선표 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : Rehmanniae Radix Preparata has been used to enrich blood and supply yin for a several thousand years. We have studied on the quantity of 5-hydroxymethyl-2-furaldehyde (5-HMF) from Rehmanniae Radix Preparata at various processings and tried to develop the making method. Methods : The Rehmanniae Radix Preparata was prepared from the steaming process with rice wine, sterilized rice wine, diluted ethanol and water each other. And the change of 5-HMF was determinated. The analysis of 5-HMF was conducted by HPLC with reversed-phase C18 column and a UV detector at 280 nm. Elution was carried out at 1.0 ml/min with 5% acetonitrile. Results and Conclusions : From this analysis, we found out that the content of 5-HMF was increased with the number of processing times and the content of 5-HMF from Rehmanniae Radix Preparata steamed for 4 times was the same as that of Korea pharmacopoeia.