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T helper 1-type immunogenicity of Mycoplasma hyopneumoniae antigen on mouse spleen cells
Hong-Gu Joo *, Seol-Hwa Yim 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the causative bacteria that can induce chronic enzootic pneumonia, resulting in low production in the swine industry. Potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia by M. hyopneumoniae has also been recognized. Although some available vaccines have been developed for prevention of M. hyopneumoniae infection, protective immunity is still poor. In this study, in order to provide valuable information on vaccine antigen, we investigated the immunogenicity of M. hyopneumoniae on mouse spleen cells. Concanavalin A (ConA) and lipopolysaccharide (LPS) were used for generation of activated T and B lymphocytes. M. hyopneumoniae made clusters of spleen cells and also affected the cellular activity and viability of spleen cells by alone or with mitogens. Of particular interest, it induced a significant increase in production of TNF-alpha in ConA-treated spleen cells, meaning T helper 1 response. In addition, cell size and mitochondrial membrane potential of M. hyopneumoniae–treated spleen cells were measured by flow cytometric analysis. M. hyopneumoniae did not affect the cell size by alone, whereas ConA or LPS profoundly increased the cell size. Taken together, M. hyopneumoniae significantly affect the cellular activity and cytokine production of spleen cells by alone or in a combination of ConA. This study provides valuable information for production of the vaccine against M. hyopneumoniae.
급성 뇌경색 환자의 관류 CT 검사 시 심방세동이 영상에 미치는 영향
홍설화(Seol Hwa Hong),문일봉(Il Bong Moon),이현성(Hyun Seong Lee),범희남(Hui Nam Beom),전주섭(Ju Seob Jeon) 대한CT영상기술학회 2012 대한CT영상기술학회지 Vol.14 No.2
I. Purpose Atrial fibrillation(A-fib) is the most common symptom at the arrhythmia. The purpose of this study was to evaluate the possibility that atrial fibrillation(A-fib) could degrade of image of Perfusion CT(PCT) on Acute Cerebral Infarction(ACI) patients. II. Meterial and Methods From January to December 2011, We had evaluated 76patient who were suspected ACI and underwent PCT at C Hospital emergency department. The patient were divided into two groups according to the A-fib(n=36), non A-fib(n=36). Heart rate was classified 2 parts Controlled Ventricular Response(CVR) and Rapid Ventricular Response(RVR) (60<HR<100.HR>100) Then we analyzed that A-fib could effect to PCT image according to heart rate. III. Result CT the 76 patients who had a ACI, 38 patients had been observed A-fib and 38 patients had not been observed. The time density curve(TDC) of A-fib groups and None A-fib groups were evaluated one-way ANOVA. We analyzed that threshold time, peak time, threshold Hounsfield Unit(HU), peak time HU, The results of analysis is that: threshold time(p=0.006), and peak time(p=0.001) were significanted. But threshold HU(p=0.517), peak HU(p=0.927) were not significanted. IV. Conclusions Therefore before PCT scan, CT Radiological technologist should recognize that the patient has A-fib or not, and scan carefully. 목적 관류 CT 영상의 질 저하의 원인 중 하나로, 부정맥에서 가장 흔한 증상인 심방세동일 가능성에 대해 연구하고, 관류 CT 영상에 얼마만큼 영향을 미치는지 알아보고자 하였다. 대상과 방법 2009년 1월부터 2011년 12월까지 C대학교 병원 응급센터에 급성 뇌경색으로 의심되어 뇌 관류 CT를 촬영한 157명의 환자 중 적응증이 된 76명의 환자를 대상으로 하였다. 심방세동 환자군이 뇌 관류 CT 영상에 영향을 주는지 확인하기 위해, 정상군과 심박동수에 따른 Controlled Ventricular Response(CVR), Rapid Ventricular Response(RVR)로 분류하여 분석하였다. 결과 급성 뇌경색 환자에서 심방세동이 관찰된 그룹과, 심방세동이 관찰되지 않은 그룹은 각각 38명이었다. 심방세동이 관찰되지 않은 대조군, CVR, RVR의 TDC에서 threshold time, peak time, threshold Hounsfield Unit(HU), peak time HU를 통계학적으로 비교 분색한 결과, threshold time과 peak time에서 각각 P-value 값이 0.006과 0.001로 유의한 차이를 보였고(P<0.05), threshold HU와 peak time HU는 각각 0.517과 0.927로 통계학적 유의한 차이는 없었다. 결론 뇌 관류 CT 검사 전에 방사선사는 심방세동 유무를 잘 확인하여 영상에 대한 문의 시 적절한 대처를 할 수 있어야 할 것으로 사료된다.
홍성진,이화준,설광열,문재유 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2
To clarify the effect of anti-juvenile hormone analogue (AJH) on the larval ecdysis by feeding at early stage of the 4th instar, the total amount of protein and activity of chitinolytic enzymes in the integument of Bombyx mori were analyzed, PAGE pattern of the protein was observed and the morphological changes of integument during molting period were also observed by means of TEM. The total amount of protein was greatly increased in premolting, then reached maximum level just before ecdysis, and rapidly decreased after the larval ecdysis in the control, while in the AJH treatment, increased 12 hr later than the control and its maximum was only 82.6% of the control. Two specific proteins, which were presumed as the protein originated from endornticle, also appeared 12 hr later than the control and were maintained to 132 hr after AJH treatment from the aspects of the Native- and SDS-PAGE patterns, although those of the control disappeared instantly after ecdysis. Chitinase and ß-N-acetylglucosaminidase activities were also suppressed and delayed by AJH treatment. Furthermore, it was observed that the apolysis took place 12 hr later than the control but new epicuticle was not formed at least until 132 hr after AJH treatment. From these results, it is suggested that the larval molting process of silkworm develops 12 hr later than the control by AJH treatment but no further processing takes place just after apolysis.
Shin, Seol Hwa,Park, Seok Soon,Lee, Kyoung Jin,Ju, Eun Jin,Park, Jin,Ko, Eun Jeong,Jung, Joohee,Kuroda, Shunich,Hong, Seung-Mo,Hwang, Jung Jin,Lee, Jung Shin,Song, Si Yeol,Jeong, Seong-Yun,Choi, Eun K Spandidos Publications 2017 ONCOLOGY REPORTS Vol.38 No.4
<P>The incidence of hepatocellular carcinoma (HCC) has continued to increase worldwide, and advanced HCC is difficult to treat using the currently available therapeutics. Chemoradiotherapy with cisplatin (cis-diamminedichloroplatinum, CDDP) is expected to confer a curative benefit on HCC patients; however, its application is limited due to side-effects such as acute nephrotoxicity as well as the conventionally limited application of chemoradiotherapy for HCC. For the practical application of this drug in the clinical setting, we formulated a novel drug carrier-comprising bio-nanocapsule (BNC) and liposomal CDDP (BNC-LP-CDDP) that recognizes the human liver and releases CDDP. BNC-LP-CDDP showed selectively high cytotoxicity for HCC cells, and markedly reduced the survival fractions of HCC when combined with ionizing radiation (IR) treatment in in vitro assays. In particular, the treatment of mice bearing human HCC with BNC-LP-CDDP and 3 Gy IR showed 95.68% growth inhibition, whereas IR treatment alone showed 65.6% growth inhibition. Moreover, BNC-LP-CDDP led to the withdrawal of CDDP-induced nephrotoxicity. These results indicate that BNC-LP-CDDP in combination with IR markedly enhanced the chemo-radiotherapeutic efficacy and eliminated CDDP induced nephrotoxicity, thus, suggesting the potential for its clinical application as human HCC therapy.</P>
Disrupted-in-schizophrenia 1 (DISC1) Regulates Dysbindin Function by Enhancing Its Stability
Lee, Seol-Ae,Kim, Seong-Mo,Suh, Bo Kyoung,Sun, Hwa-Young,Park, Young-Un,Hong, Ji-Ho,Park, Cana,Nguyen, Minh Dang,Nagata, Koh-ichi,Yoo, Joo-Yeon,Park, Sang Ki American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.11
<P>Dysbindin and DISC1 are schizophrenia susceptibility factors playing roles in neuronal development. Here we show that the physical interaction between dysbindin and DISC1 is critical for the stability of dysbindin and for the process of neurite outgrowth. We found that DISC1 forms a complex with dysbindin and increases its stability in association with a reduction in ubiquitylation. Furthermore, knockdown of DISC1 or expression of a deletion mutant, DISC1 lacking amino acid residues 403–504 of DISC1 (DISC1<SUP>Δ403–504</SUP>), effectively decreased levels of endogenous dysbindin. Finally, the neurite outgrowth defect induced by knockdown of DISC1 was partially reversed by coexpression of dysbindin. Taken together, these results indicate that dysbindin and DISC1 form a physiologically functional complex that is essential for normal neurite outgrowth.</P>