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Seokjoon Yoon,Minki Kim,Woong-Woo Lee 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.3
Background and Purpose It is challenging to detect Parkinson’s disease (PD) in its early stages, which has prompted researchers to develop techniques based on machine learning methods for detecting PD. However, previous studies did not fully incorporate the slow progression of PD over a long period of time nor consider that its symptoms occur in a time-sequential manner. Contributing to the literature on PD, which has relied heavily on cross-sectional data, this study aimed to develop a method for detecting PD early that can process time-series information using the long short-term memory (LSTM) algorithm. Methods We sampled 926 patients with PD and 9,260 subjects without PD using medicalclaims data. The LSTM algorithm was tested using diagnostic histories, which contained the diagnostic codes and their respective time information. We compared the prediction power of the 12-month diagnostic codes under two different settings over the 4 years prior to the first PD diagnosis. Results The model that was trained using the most-recent 12-month diagnostic codes had the best performance, with an accuracy of 94.25%, a sensitivity of 82.91%, and a specificity of 95.26%. The other three models (12-month codes from 2, 3, and 4 years prior) were found to have comparable performances, with accuracies of 92.27%, 91.86%, and 91.81%, respectively. The areas under the curve from our data settings ranged from 0.839 to 0.923. Conclusions We explored the possibility that PD specialists could benefit from our proposed machine learning method as an early detection method for PD.
Lee Seungjin,Reo Seung Hyeon,Kim Seokjoon,Kim Seokhwan,이은성,Cha Byung Seok,Shin Jiye,한진주,Ahn So Min,신한승,Park Ki Soo 한국바이오칩학회 2024 BioChip Journal Vol.18 No.1
In this study, Staphylococcus aureus (S. aureus ) was detected using a system that combined direct loop-mediated isothermal amplification (LAMP) and lateral flow assays (LFA). This technology relies on sequence-specific hybridization in LFA; furthermore, it has high specificity and addresses the limitations associated with nonspecific amplification in general colori- metric LAMP. In addition, a direct boiling method was adopted to streamline DNA extraction and enable simple detection. The established technology was used to successfully detect S. aureus at a concentration as low as 10 2 colony-forming unit/ mL, without cross-reactivity with other strains. The practical applicability of this technology was demonstrated by analyzing real samples such as beef jerky, cabbage, and eggshell, which were artificially spiked with S. aureus . This developed system may be beneficial with regard to operational simplicity, short analysis time, and high detection performance, which would enable its application in point-of-care settings and as a novel platform for detecting various pathogens.
12-substituted deoxoartemisinin의 합성
이석준 關東大學校 醫科大學 醫科學硏究所 1999 關東醫大學術誌 Vol.3 No.1
12-(3'-Formylbenzyl)-deoxoartemisinin(11), 12-(2'- Formylbenzyl)- deoxoartemisinin(14), a versatile intermediate of non acetal-type artemsinin derivatives with antimalarial activity, anticancer activity and so on, was synthesized from artemisinic acid(2) as a good chiral building block, which was transformed to dihydroartemisinyl aldehyde (6) by esterification, stereoselective and regioselctive reduction with NaBH4, and reduction with DIBAL-H. The C-12 substituted dihydroartemisinyl alocohol (8) and (12) given by the Crignard reaction of compound (6) with 3-vinylbenzyl magnesium bromide or butenyl magnesium bromide were changed to 12-(3'-vinylbenzyl)-deoxoartemisinin(10), 12-butenyl- deoxoartemisinin(13) by photoxidation cyclization with signlet oxygen, which was transformed to target molecules (11) and (14) with ozonolysis.
이석준,오상태,신운섭 관동대학교 의과학연구소 2001 關東醫大學術誌 Vol.5 No.1
Angiogenesis, the process of new blood capillary formation, is an important component of a number of serious disease including cancer growth and metastasis, diabetic retinopathy, rheumatoid arthritis, and many others. Coronarin A, isolated from rhizomes of the Brazilian antirheumatic medicinal plant, and synthetic related compounds showed a significant cytotoxic effect against V-79 cell and sarcoma 180 ascites in Mice. In order to evaluate antiangiogenic effect of trans decaline type labdane compounds, 3, 4a, 5, 6 and 11 were synthesized from versatile chiral building blocks scalreol 1 and sclareolide 2 with appropriate synthetic methods. All tested materials have a mild growth inhibition effect on human endothelial cell line, ECV 304. We concluded that high antiangiogenic effect of coronarin A was caused by furan aromatic functional group on C-l2 position.
이석준 關東大學校 醫科大學 醫科學硏究所 1999 關東醫大學術誌 Vol.3 No.1
8-Hydroxystdnophen(17), 8-hydroxy-4-methlsydnophen and 8-hydroxy-4-phenylsydnophen(19) were synthesized by the cyclization of related N-nitroso-α--substituted-norephendrine analongs(14, 15, 16) under the hydrogen chloride catalyst in ether at 0~5℃, which were parepared from the reaction of norephedrine, CNS stimulator, with formaldehyde, acetaldethyde or benzaldhylde containing potassium cyanide under the hydrogen sulfate. 8-Hydroxysydnocarb(1), 8-hydroxy-4-methlsydnocarb(2) and 8-hydroxy-4-phenylsydnocarb(3) with noble phenyl carbamate functional group resulting from diverse biological activity, were given from the base catalized condensation of the various 8-hydroxy-4- substituted-sydnophen(17, 18, 19) with phenylisoctanate in isopropyl alcohol. The reaction of 8- hydroxystdnophen(17) with phenyisothiocyanate tave 8-hydroxythiosysydnocarb(20). The derivatives of 4-hydroxysydnocarb and 8-hydroxy-4-methlsy-sydnocarb with 3- hydroxyl or 4- hydroxyphenyl carbamate(4, 5, 6, 7), matabolities of 8- hydroxysydnocarb(1) and 8-hydroxy-4-methlsy-sydnocarb(2), were prepared from the reaction 8-hydroxydnophen(17) and 8-hydroxy-4-methylsydnophen(18) with 3-acetoxyphenylisocyantr(29) or 4-acetoxyphenlisocyanate(30) and successive hydrolysis.
안용철,Seokjoon Lee,Cheon Soo Park,Hyuk-Jai Jang,이지환,Byong-Gon Park,Yoon Sun Park,Woon-Seob Shin,Daeho Kwon 대한독성 유전단백체 학회 2018 Molecular & cellular toxicology Vol.14 No.2
Backgrounds: Glioblastoma multiforme is one of the most aggressive human malignant brain tumors. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is known as the death ligand, which induces preferential apoptosis of transformed cancer cells. In this study, we demonstrated that the newly synthesized diethylamino-curcumin mimic with trizolyl benzene (YM-4) enhances cytotoxicity in combination with TRAIL in human glioblastoma cells. Methods: We synthesized diethylamino-curcumin mimic with trizolyl benzene (YM-4) and investigated possible apoptotic cell signaling by co-treatment with YM-4 and TRAIL on human glioblastoma cells. Results: Caspase-8, 9, and 3 and poly (ADP-ribose) polymerase were more efficiently cleaved with cotreatment of YM-4 and TRAIL than treatment with each alone in human glioblastoma cells. Co-treatment with YM-4 and TRAIL significantly increased the expression of Bax and Smac/Diablo and also inhibited the expression of the X-linked inhibitor of apoptosis protein and Survivin in human glioblastoma cells. Conclusion: These results demonstrated that YM-4 can be an anticancer candidate that can be effective on human glioblastoma cells in combination with TRAIL.