Confirmation of F1 Hybridity Using RAPD Markers in Soybean

Jong-II Chung,Mi-Suk Ko,Jung-Hyun Shim,Seok-Hyeon Kim,Jin-Ho Kang 한국자원식물학회 1999 Plant Resources Vol.2 No.1

Molecular markers are useful to confirm the hybridity of F1 plant derived from cross of two homozygous parents with similar morphological traits. RAPD markers were used to test F1 hybrid plant obtained from cross of two homozygous soybean (Glycine mar) parents. F1 plant for cross I was made from the mating of Hobbit87 (female) and L63-1889 (male) and F1 plant for cross II was obtained from the mating of H1053 (female) and L63-1889 (male). Selfing plant per each cross was also obtained. Among 20 Operon primers used, OPA04 and OPA09 show polymorphism between cross I and II parent. Band in size 1Kb of OPA04 and 2.1Kb of OPA09 primer was polymorphic band. This fragment identified F1 hybrid plant and selfing plant in cross I and II. Female parent Hobbit87 in cross I and H1053 in cross II has no this fragment (recessive allele). However, male parent L63-1889 and F1 hybrid plant in cross I and II has this size of polymorphic band (dominant allele). This indicated that F1 hybrid and selfing plants were detected by RAPD marker before phenotypic marker would be used to identify F1 hybridity. Amplification products of selfing plant for cross I and II were completely same to the those of female parent. When mature, flower color of F1 hybrid plant in cross I and II was purple and flower color of selfing plant in cross I and II was white. Purple flower is dominant trait. F1 hybridity was successfully detected at very early growth stage using RAPD marker. Therefore, RAPD marker can be used broadly to confirm F1 hybridity in many crops.

PARAMETER IDENTIFICATION OF A JOINTED BEAM STRUCTURE

김석일 한국항공대학교 1993 論文集 Vol.31 No.-

결합구조물의 진동특성은 그 구성요소와 결합부의 구조적 특성에 의히서 많은 영향을 받는다. 본 연구에서는 결합부를 4개의 구조매개변수(모멘트방향과 전단방향의 강성계수와 감쇠계수)로서 모델화하였고, 보(beam)형태의 결합구조뭉에 대한 구조매개변수들을 규명하는 방법을 제안하였다. 특히 매개변수의 정량적인 규명을 위해서 보재료의 내부감쇠특성을 고려한 Timoshenko이론에 기초를 둔 전달행렬법과 일반점성감쇠계에 대한 복소모드해석법을 이양하였다. 제안된 규명방법의 유용성은 볼트결합된 보구조물에 대한 가진실험을 통해서 입증하였다. The dyanmic characteristics of a jointed structure is determined by its structural components and joints. In the present study, the joint is modeled mathematically by four structural parameters related to the joint stiffness and damping coefficients in the moment and shear directions, and a method is proposed to systematically and quantitatively identify the parameters of a jointed beam structure which can have a relatively high natural frequency and a small length-to- depth ratio. The curve fitting technique is combined with the transfer matrix method which is based on the Timoshenko beam theory including the internal damping of the beam materials. A series of experiments for various joint conditions are performed on the bolt-jointed beam structures and compared with the analysis results. The transfer functions and mode shapes analytically calculated from the identified joint structural parameters show exellent agreements with those experimentally obtained.

설갑상선 : 1 예 보고 A Case Report

한기석,배일헌,차상훈,김성진,박길선 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

갑상선 조직 전체가 설기저부에 발생한 이소성 설갑상선 증례를 경험하였기에 그 방사선학적 소견을 문헌고찰과 함께 보고하고자 한다. 증례는 59세 여자 환자로 40 일전부터 발생한 음성 변화를 주소로 내원하였다. 이학적 검사에서 설 기지부 중앙에 회백색의 격막에 둘러 쌓인 3 cm 가량의 종괴가 있었으며, 종괴는 내장으로 돌출하여 구인두를 막고 있었다. 경부 전산화 단층촬영 측면 정찰 촬영에서, 설 기저부에 후방으로 돌출하여 구인두를 막고 있는 경계가 분명한 연부조직 종괴가 보였으며, 전산화 단층촬영 동맥기와 정맥기에 주변의 혈관과 비슷한 강하고 균일한 조영증강을 보이는 장경 2.5 cm의 원형 종괴가 있었다. 또한 갑상선이 있어야 할 경부 전방 공간에서 정상 갑상선 조직은 전혀 보이지 않았다. 설 기저부의 종괴에서 생검을 시행하였으며 병리 검사에서 갑상선 조직으로 진단되어 설갑상선으로 확진되었다. The authors report a case of ectopic thyroid that whole thyroid tissue was replaced by lingual thyroid. A 59-year-old female presented with dysphonia duration of 40 days. On physical examination, there was a 3 cm sized well encapsulated whitish mass protruding to oral cavity in milline of tongue to base. Computerized tomography of neck revealed homogeneously enhancing well marginated 2.5 cm sized round mass in the cecum of tongue base on arterial and venous phase. And there was no normal thyroid gland in lower anterior neck.

성장기 흰쥐에서 단백질 장기간 알코올 투여가 인슐린 분비능 및 감수성에 미치는 영향

윤용석,허갑범,이현철,임승길,김경래,송영득,안철우,차봉수,이해일,성제경 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.1

Background: This investigation was performed to examine the combined effects of protein malnutrition and chronic moderate amount of alcohol intake on insulin secretory capacity and sensitivity in growing rats. Methods: Weanling 4-week-old male Sprague-Dawley rats were fed low protein [5%, (wt/wt)] or control (C, 20%) diet from 4 to 12 weeks and alcohol (5 g/kg/d) or saline gavage from 8 to 12 weeks. All rats were divided into the 4 groups according to different diet protocols: group I (protein-deficient alcohol rats), group II (protein-deficient saline rats), group III (protein-sufficient alcohol rats), and group IV (protein-sufficient saline or control rats). At the age of 12 weeks, we determined the insulin secretory capacity and sensitivity in the 4 different diet groups. Results: The results are summarized as following; 1. Normal weight gain was nearly completely arrested in protein-deficient rats compared to control rats. In protein-sufficient rats, chronic alcohol intake decreased body weight gain. Pancreatic weight adjusted with body weight was not different among the 4 groups, but epididymal fat weight adjusted with body weight was decreased in group II compared to group IV. 2. Intraperitoneal glucose tolerance was improved in group I compared to the other groups. Insulin responses to glucose challenge were markedly decreased in group II compared to group IV, but not in group I. 3. Glucose disposal rate during euglycemic clamp test was diminished in group II compared to group IV, but there were no differences between group I and group IV. Glycogen synthase activities of skeletal muscle after 2 hour hyperinsulinemic state were not different among the 4 groups. 4. There were no differences of reserved insulin content of whole pancreas adjusted with pancreas weight among the 4 groups. 5. In light microscopic findings of pancreatic islets, sizes of islets, islet cells and nuclei were decreased in protein-deficient rats compared to control rats. However, the sizes of islet cells and nuclei were further decreased in group II compared to group I. Conclusion: These results suggest that impaired insulin secretion and decreased insulin sensitivity due to protein malnutrition can be restored by chronic, moderate amount of alcohol intake, but these beneficial effects may not be appeared in protein-sufficient state. Therefore, the chronic alcohol intake differently influences glucose metabolism according to individual nutritional status, and further studies for the effects of alcohol intake in lean diabetic patients are required to extrapolate these results in human.

Antinociceptive Effects of Intrathecal Metabotropic Glutamate Receptor Compounds and Morphine in Rats

Choi, Jeong II,Lee, Hyung Kon,Chung, Sung Tae,Kim, Chang Mo,Bae, Hong Beom,Kim, Seok Jai,Yoon, Myung Ha,Chung, Sung Su,Jeong, Chang Young The Korean Pain Society 2005 The Korean Journal of Pain Vol.18 No.1

Background: Spinal metabotropic glutamate receptors (mGluRs) and opioid receptors are involved in the modulation of nociception. Although opioid receptors agonists are active for pain, the effects of the compounds for the mGluRs have not been definitely investigated at the spinal level. We examined the effects of the intrathecal mGluR compounds and morphine in the nociceptive test, and then we further clarified the role of the spinal mGluRs. In addition, the nature of the pharmacological interaction after the coadministration of mGluRs compounds with morphine was determined. Methods: Catheters were inserted into the intrathecal space of male SD rats. For the induction of pain, $50{\mu}l$ of 5% formalin solution or a thermal stimulus was applied to the hindpaw. An isobolographic analysis was used for the evaluation of the drug interaction. Results: Neither group I mGluR compounds nor group III mGluR compounds produced any antinociceptive effect in the formalin test. The group II mGluR agonist (APDC) had little effect on the formalin-induced nociception. The group II mGluR antagonist (LY 341495) caused a dose-dependent suppression of the phase 2 flinching response on the formalin test, but it did not reduce the phase 1 response of the formalin test nor did it increase the withdrawal latency of the thermal stimulus. Isobolographic analysis revealed a synergistic interaction after the intrathecal delivery of a LY 341495-morphine mixture. Conclusions: These results suggest that group II mGluRs are involved in the facilitated processing at the spinal level, and the combination of LY 341495 with morphine may be useful to manage the facilitated pain state.

CIR - Tree를 위한 효율적인 대량적재 알고리즘의 설계 및 구현

피준일(Jun Ii Pee),송석일(Seok Ii Song),유재수(Jae Soo Yoo) 한국정보과학회 2002 정보과학회논문지 : 데이타베이스 Vol.29 No.3

이 논문에서는 고차원 색인 구조인 CIR-트리를 위한 효율적인 벌크로딩 알고리즘을 설계하고 구현한다. 벌크로딩 기법은 대량의 고차원 데이타가 색인 구성 시 함께 주어지는 경우 색인의 구성을 빠르게 하고 구축한 색인의 검색 성능을 향상시킨다. CIR-트리는 반드시 필요한 차원만 이용해서 비단말 노드의 엔트리를 구성하기 때문에 엔트리 크기가 일정하지 않다. 이 특성은 비단말 노드의 분기율을 높이고 탐색 성능을 향상시키는 효과가 있다. 기존에 다차원 및 고차원 색인구조를 위한 벌크로딩 기법이 제안되었지만 이러한 CIR-트리의 특징을 제대로 살릴 수 있는 방법은 없다. 이 논문에서는 기존의 벌크로딩 알고리즘을 개선하면서 CIR-트리의 특징을 효과적으로 색인 구성에 반영할 수 있는 알고리즘을 제안한다. 또한 이를 BADA-Ⅲ의 하부 저장 시스템인 MiDAS-Ⅲ에서 구현하고 다양한 실험을 통해 그 성능을 입증한다. In this paper, we design and implement an efficient bulk-loading algorithm for CIR-Tree. Bulk-loading techniques increase node utilization, improve query performance and reduce index construction time. The CIR-tree has variable size of internal node entries since it only maintains minimal dimensions to decriminate child nodes. This property increases fan-out of internal nodes and improves search performance. Even though several bulk-loading algorithms for mutli/high-dimensional index structures have been proposed, we cannot apply them to CIR-tree because of the variable size of internal node entries. In this paper, we propose an efficient bulk- loading algorithm for CIR-tree that improves the existing bulk-loading algorithm and accomodates the property of CIR-tree. We also implement it on a storage system MiDAS-Ⅲ and show superiority of our algorithm through various experiments.

수산계 대학 어선항해사 지정교육기관 교과편성 분석

김형석(Hyung-Seok KIM),강일권(II-Kwon KANG),김욱성(Wook-Sung KIM),이유원(Yoo-Won LEE),김석재(Seok-Jae KIM),류경진(Kyung-Jin RYU) 한국수산해양교육학회 2014 水産海洋敎育硏究 Vol.26 No.5

텍스트 문서 영상 컨텐츠의 부활

오일석(Oh II-Seok) 한국콘텐츠학회 2003 한국콘텐츠학회 종합학술대회 논문집 Vol.1 No.2

인류의 지식은 문자와 문서라는 형태를 통해 오랜 세월 축적되어 왔다. 최근 컴퓨터 기술과 인터넷의 발전으로 인해 문서의 제작과 제공 패러다임이 아날로그 방식에서 디지털 방식으로 급속히 바뀌고 있다. 이러한 과정에서 기존 컨텐츠는 아날로그 형태로 존재하나 새로 발생하는 컨텐츠는 디지털 형태를 갖게 되므로 존재 방식에 큰 간격이 발생하여 여러 문제를 야기할 수 있다. 이 논문은 텍스트 문서 영상 컨텐츠에 대해 이러한 간격을 줄이기 위한 방안을 고찰한다. The human knowledge has been integrated mainly through the text documents. The computer technologies changed the way of production and deliverly of the documents from analog to digital. During the paradigm shift, a serious problem must occur due to a large gap between the old contents and newly generated contents. This paper reviews some methods to reduce the gap for the text document image contents.

음운인식 진단·평가 모형 개발

석동일(Dong II Seok) 한국언어청각임상학회 2006 Communication Sciences and Disorders Vol.11 No.3

최근 들어 음운인식은 언어장애 아동의 문해력(literacy) 기술 뿐만 아니라 언어능력 발달을 평가하는 데 있어서 중요한 요소로 밝혀지고 있다. 이러한 이유로 국외에서는 표준화된 여러 검사 도구들로 음운인식 능력을 진단․평가하고 있다. 그러나 현재 국내에는 표준화된 검사 도구가 없어 음운인식 진단․평가가 어렵다. 따라서 본 연구는 음운인식 진단․평가 모형 개발을 목적으로 음운인식 검사 도구에 포함되어있는 하위 과제 유형과 수, 과제별 문항 수, 과제 수행 방법 그리고 음운인식 진단․평가의 부가적인 정보 제공을 위해 포함되어 있는 평가 영역을 분석하였다. 연구 결과 첫째, 국내외적으로 음운인식 검사 도구의 하위 과제 유형에는 음절, 음절체-각운, 음소 순으로 변별, 분절, 탈락, 합성이 주요한 것으로 나타났다. 특히 국내 음운인식 검사 도구의 하위 유형에는 음소수준의 초성, 중성, 종성 변별이 국외 검사 도구와 달리 주요한 하위 과제 유형으로 나타났다. 하위 과제의 수는 검사 도구의 목적에 따라 차이가 있으나 4~10개이며, 과제별 문항 수는 음절 및 음절체-각운 보다 음소수준에서 더 많았다. 과제 수행 방법은 대부분 피검자의 산출 반응을 요구하는 것으로 나타났다. 둘째, 음운인식 진단․평가의 부가적인 정보 제공을 위해 포함되어 있는 평가 영역은 음운재부호화(phonological recoding), 음운부호재생(retrieval of phonological code), 글자지식(letter knowledge), 읽기 그리고 쓰기로 나타났다. 결론적으로 음운인식 진단․평가를 위해 우선적으로 음운인식 검사 도구를 개발하되 이상의 구성적 및 방법적 특징을 고려해야 한다. The phonological awareness refers to the ability to recognize that speech is made up of sentences that can be broken down into words, syllables, intrasyllabic units, and phonemes and is the ability to talk about, reflect upon, and manipulate these components. Phonological awareness ability has naturally been associated with the ability to read and spell. Therefore, the American Speech-Language-Hearing Association(ASHA) asserted that speech-language pathologist should take an important role in promoting literacy for these children. Nevertheless, we lack a standardized assessment test of phonological awareness. The purpose of this study was to propose an evaluation and assessment model for phonological awareness. We primarily analyzed the task of phonological awareness, number of tasks, number of items each task and children response acts followed direction, and subsequently investigated other factors that might be associated with phonological awareness. The conclusion of this study were as follows. First, the common tasks of phonological awareness were discrimination, segmentation, deletion and blending in syllable, rhyme and phoneme level. Particularly, in Korea, tasks examined initial consonants, middle vowel, and final consonants discrimination. Common tasks employed 4~10 tasks and production response. More items were included at phoneme level tasks than at syllable level. Second, additional tasks examined phonological recoding, retrieval of phonological code, letter knowledge, reading and writing.

요통환자에 있어서 MRI 소견상 추간판 팽윤의 임상적 의의

선호영,조성일,이정웅,임상덕,정상돈,윤영석 최신의학사 1993 最新醫學 Vol.36 No.12