한우 성별에 따른 등심의 육질특성 비교

최정석, 정동순, 최석현, 이상화, 김종희, 최양일 忠北大學校 農業科學硏究所 2011 農業科學硏究 Vol.27 No.4

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지문정보를 이용한 안전한 암호키 저장 시스템 개발

최은화,서창호,임종태,김석우 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-

Biometric encryption refers to a process of secure key management. Biometric encryption does not directly provide a mechanism for the encryption/decryption of data, but rather provides a replacement to typical passcode key-protection protocols. Although the process of biometric encryption can be applied to any biometric image, the initial implementation was achieved using fingerprint images.

Medetomidine을 투여한 개의 진정효과와 혈액상 변화

崔錫和 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

This study was performed to revaluate the effect of medetomidine HCl as a sedative in dogs. Clinical findings, hematological and blood chemical values were determined after intramuscular injection of medetomidine HCl at a standard dosage of 0.04 mg/kg body weight in clinically healthy dogs. Sedative induction and sedation times were observed. Hematological values, total protein (T.P), glucose, aspartate aminotransferase(AST), alanine aminotransferase(ALT) and blood urea nitrogen(BUN) were analyzed in the condition of 0-, 0.5-, 1- and 3- hour after administration of drug. The results obtained in the study were as follows ; 1. After injection of medetomidine, all dogs became handlable and the degree of muscle relaxation was good. 2. Sedative induction and sedation times were 7.1 and 58.9 minitues in dogs administered with medetomidine , respectively. 3. Three of 5 dogs administrated with medetomidine showed retch during induction period. 4. Dogs showed urine excretion and mild muscle tremor on recovery time. 5. After injection, heart beat and respiratory rate were greatly decreased (p ≤ 0.01) but body temperature was almost unchanged. 6. Changes of red blood cells, hemoglobin, hematocrit and white blood cells were not significant during or after medetomidine treatment. 7. Serum glucose values were getting increased after medetomidine injection for at least 3 hours. 8. No changes of T.P, AST, ALT and BUN values were observed during or after medetomidine treatment. Conclusively, treatment of medetomidine produced remarked sedative effect in dogs. No changes in hematological and blood chemical value(T.P, AST, ALT, BUN) were observed.

Aspergillus oryzae를 이용한 캡슐형 메주의 품질특성

최재훈,권선화,이상원,남상해,최상도,박석규 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

전통메주의 표면에서 발행하는 야생곰팡이 착생, 유해곰팡이에 대한 mycotoxin 생성 및 메주 표면의 과도한 건조 등을 해결하기 위하여 메주 외층에 Aspergillus oryzae로 만든 쌀 종국(0.3%, w/w)을 혼합시킨 증자 검정콩으로 얇게 코팅한 후, 25℃, 상대습도 80%에서 14일간 발효시킨 캡슐형 메주를 개발하였다. 캡슐형 메주의 표면에서 야생곰팡이의 오염은 발견되지 않았으나, 대조구 메주는 12일 이후에 푸른곰팡이가 많이 관찰되었다. 메주의 수분 함량은 평균 32.7%로서 34.7~29.4%의 범위를 나타내었다. 적정산도는 캡슐형 메주가 대조구 메주보다 높게 나타났으며, 대두 캡슐형 메주와 검정콩 캡슐형 메주는 비슷하게 나타났다. 유리당의 함량은 검정콩 캡슐형 메주가 123.98㎎%로 대조구 메주 15.02㎎%와 대두 캡슐형 메주 59.85㎎%보다 각각 10배, 2.1배, 높게 나타났다. 아미노산성 질소의 함량은 대조구 메주에서 147.00㎎%였으며, 검정콩 캡슐형 메주는 255.50㎎%로서 대두 캡슐형 메주 187.25㎎%보다 약 1.37배 높은 함량을 나타내었다. 총 유기산의 함량은 캡슐형 메주(대두 119.98㎎%, 검정콩 95.94㎎%)가 대조두 메주(26.44㎎%)보다 함량이 높게 나타났으며, 캡슐화에 따라 lactic acid와 malic acid 가 가장 큰 변화를 나타내었다. 색차계 색도는 검정콩 캡슐형 메주가 대두 캡슐형 메주보다 명도값(L)이 크게 나타났으며 지방산 조성은 메주의 종류에 따라 큰 차이를 나타내지 않았다. 총 유리 아미노산의 함량은 검정콩 캡슐형 메주(1039.70㎎%)가 대두 캡슐형 메주 (556.07㎎%)와 대조구 메주(236.45㎎%) 비하여 각각 2.4배, 4.4배 높았다. In order to improve some problems such as contamination of undersirable mold, mycotoxin production and excessive drying on the surface of traditional meju, we developed a capsule type-meju(CM) coated with steamed black bean and Aspergillus oryzae - rice koji(0.3%, w/w) mixture to surface of traditional meju and fermented at 25℃ for 14 days under 80% relative hunmidity. Contamination of undersirable mold on the surface of CM was not detected within 2 weeks and some genus Penicillium molds on control meju without koji were found naturally after 12 days of fermentation. The moisture content of meju was showed to be in the range of 34.7 - 29.4% being 32.7(w/w) of mean value. Titratable acidities in CMs prepared with black bean(BCM) and soybean(SCM) were much higher than that in control meju, and BCM was similar to SCM. Free sugar content in BCM(123.98 ㎎%) was 10 times and 2.1 times higher than that in control meju(15.02 ㎎%) and SCM(59.85㎎%), respectively. Amino type nitrogen content in control meju was 147.00 ㎎% and its content in BCM(255.50㎎%) was 1.37 times higher than that in SCM(187.25 mg%). Total organic acid content in BCM(95.98㎎%) and SCM(119.98㎎%) were much higher than that in control meju(26.44 ㎎%), and then latic and malic acid contents were markedly changed according to capsulation of meju. Lightness value(l) of Hunter color index was much higher in BCM than in SCM. Fatty acid composition of CM was not different as compared to control meju. Total free amino acid content in BCM(1039.70 ㎎%) was 4.4 times and 2.4 times higher than that in control meju(236.45 ㎎%) and SCM(556.07 ㎎%), respectively.

犬에서 medetomidine hydrochloride의 臨床應用에 關한 硏究

崔錫和 건국대학교 1989 論文集 Vol.29 No.2

This study was performed to evaluate the effect of medetomidine hydrochloride as a sedative and analgesic in dogs. Clinical findings, hematological and blood chemical values were determined after intramuscular injection of 0.1% medetomidine hydrochloride at the dose of 0.04mg/kg body weight in clinically healthy crossbred dogs. The observation was performed before, 0.5, 1 and 3hours alter injection. The results obtained were as follows ; 1) Three of 5 dogs were retched, and showed urine excretion and muscle jerkling near to the convalescent stage. 2) At the 0.5, 1 hour after injection, heart beat and respiratory rate were greatly decreased (P<0.01) but body temperature was almost unchanged. 3) White blood cell, red blood cell, hemoglobin concentration and hematocrit value were slighter decreased during sedation, but these values were returned to normal after 3 hours. 4) Serum bilirubin content was slightly increased 0.5 and 1 hour after injection, blood urea nitrogen and electrolytes(Na, K, Cl) made little difference between before and after injection. Creatinine value was increased in order of 0.5, 1 and 3 hours after injection. Iron was decreased 0.5 and 1 hour after injection but increased 3 hours after injection(P<0.01). Calcium was decreased 3 hours after injection.

건강인에서 결핵균 30-kDa항원이 말초혈액 임파구증식에 미치는 영향

최대경,고석신,김화중,조은경,백태현,신철식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

T lymphocytes are thought to play a central role in cell mediated immune response. To Study the T lymphocyte proliferative response to 30-kDa antigen purified from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes isolated from healthy subjects were stimulated with the 30kDa antigen, crude antigen or PHA. Healthy subjects were divided into three groups so that PPD(+), PPD(±) and PPD(-). The proliferations of lymphocytes were measured by the incroporation of ^3H-thyrnidine. The results were summarized as follows: 1. For the PPD(+) person, at concentration of < 1 ㎍/ml, the proliferative response to 30-kDa antigen resulted in a mild reduction but crude antigen showed relatively rapid reduction. For the PPD(-) person, decreasing the concentration of the both antigens showed rapid reduction of proliferation 2. The lymphocytes proliferations to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. 3. The sensitivity and specificity of lymphocyte proliferation assay to 30-kDa antigen and crude antigen when compared with skin test results were 92.3%, 100% and 76.9%, 50%, respectively.

원료콩의 증자조건을 달리한 검정콩 간장의 유리당, 유기산 및 지방산의 변화

권선화,최재훈,고영란,손미예,박석규 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

콩의 증자조건에 따른 검정콩 전통간장의 숙성 후 유리당, 유기산, 지방산조성을 조사하였다. pH 값은 5.04~5.32로 시험구간의 큰 차이를 나타내지 않았고, 원료 콩을 상압증자한 검정콩 간장이 가압이나 스팀보다는 약간 높은 수치를 나타내었다. 적정산도는 4.26~7.73mL로 나타났으며, 가압증자한 검정콩 간장(4.26~5.61mL)이 가장 높았으며, 다음으로 상압(7.32mL), 스팀(5.99~6.96mL)의 순으로 나타났다. 환원당은 25.18~194.81㎎%로 나타났고, 상압이 25.18㎎%로 가장 낮았으며, 가압이나 스팀증자한 검정콩 간장의 경우 상호간에는 큰 차이가 없지만, 상압한 것보다는 7~8배 높은 함량을 나타내었다. 주요 유리당은 glucose, fructose, xylose였으며, 스팀증자한 검정콩 간장이 상압이나 가압보다 각각의 유리당 모두가 많았고, 상압증자는 유리당이 극히 미량으로 검출되었다. 주요 유기산은 succinic acid, oxalicaicd, tartaric aic, malic acid, citric acid였고, 총 유기산은 스팀증자 (40.38~76.96 ㎎%)한 검정콩 간장이 상압(55.85㎎%)이나 가압(27.78~34.21)한 것보다 많았다. 증자 처리시간이 길수로 가압과 스팀으로 처리하여 제도된 검정콩 간장은 약간씩 감소되는 경향을 나타내었다. 주요 지방산은 palmitic acid(33.28~43.51%), stearic a창(14.15~19.16%), myristic acid(5.03~19.14%)였으며, 전체 지방산 중에 55.97~72.85% 비율을 차지하였고, 원료 콩에 존재하지 myristic acid가 상당히 많이 존재하였다. pH values in all samples were in the range of 5.04 to 5.32. pH of kanjang prepared with normal pressure(HPK) was higher than that of kanjang prepared with high pressure(HPK) and kanjang prepared with steam(SPK). Titratable acidity(TA) in all samples were found to be 4.26∼7.72 mL. TA in HPK(5.99∼6.96 mL) was lower than that in SPK(4.26∼5.61 mL) and NPK(7.32 mL). Reducing sugar(RS) contents in all samples were in the range of 25.48 to 194.81 ㎎%. Rs contents of HPK and SPK were a similar pattern and were higher than that of NPK. Major free sugars(FS) were glucose, fructose and xylose. In total FS, SPK was higher than that of NPK and HPK. Major organic acids(OA) were succinic acid, oxalic acid, tartaric acid, malic acid citric acid. OA contents in HPK and SPK were decreased slightly according to the heating time of whole black bean. The highest percentage of fatty acid(FA) in all samples was palmitic acid(33.28∼43.51% as area % of GC chromatogram), followed in a decreasing order by stearic acid(14.15∼19.16%) and myristic acid(5.93∼19.14%). The composition ratios of three FA to total FA ranged from 55.97 to 72.85%. In all samples, there was myristic acid that was not contained in raw soybean.

EMLA Cream 도포와 1% Lidocaine 침윤 후 요골동맥천자시 진통정도의 비교

윤석화,황원재,최세진 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

In a double blind study, the efficacy of topical 5% EMLA cream was compared with that of 1% lidocaine infiltration in the pain-relief and incidence of complication after radial arterial cannulation. Forty three premedicated adults were allocated randomly to two groups to receive EMLA cream and 1% lidocaine infiltration. Following arterial cannulation, pain was assessed by the patient using visual analogue score (VAS) and by a independent observer using four-caregory verbal rating score (VRS). There no significant differences between the EMLA group and 1% lidocaine infiltration group both patient and observer assessments. Compared with lidocaine infiltration group, significantly lower trial numbers of puncture in those recieving EMLA cream group, but no difference of discomfortness of puncture were observed in EMLA cream group.

Neuropathic Pain의 동물모델에서 유발된 Thermal Hyperalgesia에 대한 Clonidine의 효과

윤석화,오세철,최세진 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The effects of intraperitoneal injection(i.p.) of the alpha 2 adrenal receptive agonist, clonidine on the thermal hyperalgesia produced by unilateral patially tight ligation of the sciatic nerve was studied in rats. First in the preoperative and postoperative Mays, paw withdrawal latency of both hind paw(lesioned,unlesioned) with heat stimulant was tested. After that, each groups of rats injected saline, clonidine 10㎍/kg, or clonidine 50㎍/kg. I.p.10㎍/kg and 50㎍/kg clonidine, paw withdrawal latency of lesioned side significantly reduced than saline group. It is suggested systemical clonidine may be useful in treating neuropathic pain in humans.

결핵균 α-항원의 정제와 면역화학적 분석에 관한 연구

김석권,백태현,김화중,박정규,최대경 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

α-antigen from the culture filtrate of Mycobacterium tuberculosis H37Rv was purified by improved purification procedures and immunoaffinity chromatography using rabbit anti-α serum. Improved purification procedures were involved 50% ammonium sulfate precipitation, hydroxylapatite chromatography, Sephadex G-75 chromatography and preparative natural polyacrylamide gel electrophoresis(PAGE). We also produced seven monoclonal antibodies against α-antigen by hybridoma technique and analyzed immunochemical characterization of α-antigen by SDS-PAGE, ELISA and Western bolt using monoclonal antibodies. The results were summarized as follows; 1. The purification of α-antigen from the unheated culture filtrate by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 chromatography resulted in recovery of 16% of α-antigen and 28.5-fold purification. α-antigen purified further by preparative natural-PAGE showed a single distinctive band of 30 KDa molecule on SDS-PAGE. 2. The molecular weight of α-antigen was 30,000 as determined by SDS-PAGE and Sephadex G-75 chromatography. 3. α-antigen was purified by immunoaffinity chromatography as single step. The affinity purified α-antigen showed a band of 30 KDa molecule and showed another contaminated band of 32 KDa molecule on SDS-PAGE. 4. By Western blot analysis, seven monoclonal antibodies against α-antigen showed a distinct band of 30 KDa molecule and multiple different bands between 25 KDa and 65 KDa molecules. 5. All monoclonal antibodies produced were revealed strong cross reactivities with the sonicate antigens of slow growing and rapid growing mycobacteria by ELISA. These results suggest that α-antigen, 30 KDa of molecular weight, could be purified well by the im-proved above purification procedures and contains many cross-reactive antigenic determinants on the molecule.