한우의 혈액단백질 및 효소의 유전적 다형현상

상병찬,신형두,류승희,강흥주 한국축산학회 1996 한국축산학회지 Vol.38 No.4

To investigate the genetic polymoiphisms and constitutions of blood proteins and enzymes in the Korean cattle population of Chungnam, Provincial Animal Breeding Station(CPABS) and National Livestock Cooperatives Federation(NLCF), the genetic variants of transferrin(Tf), post-transferrin-2 (pTf-2), albumin(Alb), posta-albumin(pAlb), ceruloplasmin(Cp), amylase-I (Am-I) and hemoglobin(Hb) were analyzed using the PAGE (polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis) methods. On the genetic variants of the serum proteins, the transfecrin(Tf) locus was assumed to be genetically controlled by codominant alleles, Tf A, D₁, D₂, E and K, and the gene frequencies of these were 0.299, 0.331, 0.160, 0.207 and 0.001, respectively. The post-transferrin locus was observed to be controlled by pTf-2 F and S alleles, and the gene frequencies of these were 0.686 and 0.314, respectively. The albumin(Alb) and post-albumin(pAlb) loci were identified to be controlled by two alleles, Alb A and B alleles for Alb locus, pAlb F and S alleles for pAlb locus, and the gene frequencies of these were 0.999 and 0.001 for Alb A and B, 0.440 and 0.560 for pAlb F and S apes, respectively. On the genetic variants of the semm enzymes, cetuloplasmin(Cp) and amylase-I(Am-1) loci were found to be controlled by two alleles, Cp F and S for Cp locus, and Am-1 B and C for Am-1 locus, and gene frequencies of these were 0.420 and 0.580 for Cp F and S, 0.417 and 0.583 for Am-1 B and C, respectively. On the genetic variants of the hemoglobin(Hb), the distributions of genotypes were 84.25, 14.96 and 0.79% for Hb AA, AB and BB types, and the gene frequencies for Hb A and B were 0.917 and 0.083, respectively.

HBV : PE-028 ; Prevalence of hepatitis A, B, C and D viruses among patients with acute hepatitis in Mongolia

( O. Baatarkhuu ),( Do Young Kim ),( B Bayarmagna ),( N Khorolsuren Baigal ),( R Ouyngerel ),( D Enkhsaikhan ),( Y Dahgwahdorj ),( Sang Hoon Ahn ),( Kwang Hyub Han ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background and Aims: Mongolia is an endemic area of acute and chronic viral hepatitis. To determine the prevalence of HAV, HBV, HCV and HDV infections among patients with acute hepatitis in Mongolia during outbreaks. Methods: A total of 624 patients (353 males and 271 females; age, 18.6±8.1 years; range 2-66 years) who were clinically diagnosed with acute hepatitis during outbreak from October 2011 to March 2012 in Ulaanbaatar, Mongolia have been studied. The prevalence of hepatitis virus infections was determined by testing of serum for the IgM class of antibodies against HAV, HBV, HCV, HDV, and HBsAg. Results: Acute hepatitis A (AHA) (IgM anti-HAV positive) was diagnosed in 284 patients (45.5%). Acute hepatitis B (AHB) (IgM anti-HBc positive) was diagnosed in 191 patients (30.6%) and 22 patients (3.5%) was diagnosed as co-infection of B and D. Acute hepatitis C (AHC) (IgM anti-HCV positive) was diagnosed in 33 patients (5.3%). There were 52 (8.3%) HBV carriers who had detectable HBsAg and anti-HDV but who were negative for both IgM anti-HAV and IgM anti-HBc, suggesting that they acquired type D acute hepatitis due to superinfection of HDV on a background of chronic HBV infection. Forty-two (6.5%) patients were found to be infected by various combinations of dual viruses such as HAV/HBV (2.8%), HAV/HDV (0.6%), HBV/HCV (2.5%), and HCV/HDV (0.6%). AHA was the most prevalent in subjects aged 1-19 years, AHB and HBV+HDV superinfection were the first and second most prevalent in the age group of 20-29 years, and AHC was the most common type in 40-49 years. Conclusion: Dual hepatitis viral infection was detected in approximately 6.5% among patients with acute hepatitis in Mongolia.

HBV : PE-028 ; Prevalence of hepatitis A, B, C and D viruses among patients with acute hepatitis in Mongolia

( O Baatarkhuu ),( Do Young Kim ),( B Bayarmagnai ),( N Khorolsuren ),( B Baigal ),( R Ouyngerel ),( D Enkhsaikhan ),( Y Dahgwahdorj ),( Sang Hoon Ahn ),( Kwang Hyub Han ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background and Aims: Mongolia is an endemic area of acute and chronic viral hepatitis. To determine the prevalence of HAV, HBV, HCV and HDV infections among patients with acute hepatitis in Mongolia during outbreaks. Methods: A total of 624 patients (353 males and 271 females; age, 18.6±8.1 years; range 2-66 years) who were clinically diagnosed with acute hepatitis during outbreak from October 2011 to March 2012 in Ulaanbaatar, Mongolia have been studied. The prevalence of hepatitis virus infections was determined by testing of serum for the IgM class of antibodies against HAV, HBV, HCV, HDV, and HBsAg. Results: Acute hepatitis A (AHA) (IgM anti-HAV positive) was diagnosed in 284 patients (45.5%). Acute hepatitis B (AHB) (IgM anti-HBc positive) was diagnosed in 191 patients (30.6%) and 22 patients (3.5%) was diagnosed as co-infection of B and D. Acute hepatitis C (AHC) (IgM anti-HCV positive) was diagnosed in 33 patients (5.3%). There were 52 (8.3%) HBV carriers who had detectable HBsAg and anti-HDV but who were negative for both IgM anti-HAV and IgM anti-HBc, suggesting that they acquired type D acute hepatitis due to superinfection of HDV on a background of chronic HBV infection. Forty-two (6.5%) patients were found to be infected by various combinations of dual viruses such as HAV/HBV (2.8%), HAV/HDV (0.6%), HBV/HCV (2.5%), and HCV/HDV (0.6%). AHA was the most prevalent in subjects aged 1-19 years, AHB and HBV+HDV superinfection were the first and second most prevalent in the age group of 20-29 years, and AHC was the most common type in 40-49 years. Conclusion: Dual hepatitis viral infection was detected in approximately 6.5% among patients with acute hepatitis in Mongolia.

Observation of a d-wave gap in electron-doped Sr₂IrO₄

Kim, Y. K.,Sung, N. H.,Denlinger, J. D.,Kim, B. J. NATURE PUBLISHING GROUP 2016 NATURE PHYSICS Vol.12 No.1

<P>High-temperature superconductivity in cuprates emerges out of a highly enigmatic 'pseudogap' metal phase. The mechanism of high-temperature superconductivity is probably encrypted in the elusive relationship between the two phases, which spectroscopically is manifested as Fermi arcs-disconnected segments of zero-energy states-collapsing into d-wave point nodes upon entering the superconducting phase. Here, we reproduce this distinct cuprate phenomenology in the 5d transition-metal oxide Sr2IrO4. Using angle-resolved photo-emission, we show that the clean, low-temperature phase of 6-8% electron-doped Sr2IrO4 has gapless excitations only at four isolated points in the Brillouin zone, with a predominant d-wave symmetry of the gap. Our work thus establishes a connection between the low-temperature d-wave instability and the previously reported high-temperature Fermi arcs in electron-doped Sr2IrO4 (ref. 1). Although the physical origin of the d-wave gap remains to be understood, Sr2IrO4 is the first non-cuprate material to spectroscopically reproduce the complete phenomenology of the cuprates, thus offering a new material platform to investigate the relationship between the pseudogap and the d-wave gap.</P>

한국 재래 닭 품종 특성 및 초기성장 개량을 위한 분자표지 개발

오재돈,박미현,공홍식,이학교,전광주,연성흠,상병돈,최철환,조병욱,Oh J. D.,Park M. H.,Kong H. S.,Lee H. K.,Jeon G. J.,Yeon S. H.,Sang B. D.,Choi C. H.,Cho B. W. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.1

This study was conducted to estimate the effects of genotype for chicken major histocompatibility complex (MHC) B-LB genes on economic traits. To detect polymorphism, 400 bp fragments of MHC B-LB genes were obtained and sequenced. After digestions using restriction enzyme Hea III, two restriction enzyme sites were observed. There were two mutations at position 427 and 651 those were decided as Type I and Type II, respectively. Using RFLP analyses, type I were genotyped to TT, TC and CC, and type II to MM, Mm and mm. The relatively higher TC genotype frequencies (0.8) of Type I and Mm genotype frequencies (0.88) of Type II were observed in Korean native chickens. The effects of the genotype on 150 days body weight trait were investigated by the associations of CC and Mm genotypes (P<0.05) in Korean native chickens. This result suggests that a significant association exists between the SNP and 150 days body weight. 본 연구는 한국 재래 닭의 유전적 특성을 분자표지를 이용하여 그 차이를 규명하고 초기성장에 미치는 영향을 분석하여 이를 이용한 재래닭의 개량을 목적으로 실시하였다. MHC class II B-LB 유전자 내의 염기변이체가 경제형질에 미치는 영향에 대하여 연구하였다. MHC class II B-LB유전자 내 400 bp 크기의 유전자를 증폭하여 염기서열 분석과 제한효소 처리를 이용한 다형성 분석을 실시하였다. 연구결과 두 개의 제한효소 절단지역이 발견되었으며 427 지역을 Type I 으로 651 지역은 Type II로 정하여 RFLP 분석을 실시하였다. Type I지역의 유전자형은 TT, TC, CC로 나타났으며, TypeII 지역의 유전자형은 MM, Mm, mm으로 나타났다. TC와 Mm 유전자형이 다른 유전자형과 비교하였을 때 한국재래 닭에서 높은 출현빈도를 보였다(0.8, 0.88). 유전자형이 한국 재래 닭의 150일령 체중에 미치는 영향을 분석한 결과 CC와 Mm 유전자형에서 통계적 유의성이 도출되었다 (P<0.05). 따라서 본 연구의 결과를 이용하여 한국 재래 닭의 유전적 특성을 규명할 수 있으며 초기 성장이 높은 성적을 나타내는 CC, Mm 유전자형을 개량에 이용하게 된다면 큰 효과를 얻을 수 있을 것으로 사료되어진다. 본 연구의 결과는 차후 한국 재래 닭의 과학적이고 지속적인 유전자원의 보존과 육종 전략에 있어 매우 유용하게 활용될 것으로 기대된다.

재래산양 락토페린 유전자 5' Flanking 영역의 특성에 관한 연구

상병찬,유대열,유성란,이태훈,신상태,이경광 한국축산학회 2000 한국축산학회지 Vol.42 No.1

Lactoferrin(Lf), a ferric ion(Fe^(3+))-binding glycoprotein, is found most notably in milk and some exocrine secretions. It is a main antimicrobial component of colostrum and milk that contributes to protection of the infant against infectious diseases. To analyze 5' flanking region of Korean native goat lactoferrin gene, we cloned and sequenced 975 by of 5' flanking region of Korean native goat Lf gene. When compared to those of bovine and human Lf, the nucleotide sequences exhibited about 90.9% and 49.7% homology, respectively. The transcription start site was localized by primer extension to residue C, which is 38 nucleotides upstream from the ATG start codon. The 5' flanking region of Korean native goat lactoferrin gene contains putative transcription factor binding sites such as SP1, CAT, C/EBP and GATA-1.

Graves 병 안구증에서 99mTc-DTPA 뇌신티그라피를 이용한 안구/뇌 방사능비에 관한 연구

이범우,성상규,박원,서관식,김덕주,김종순 대한핵의학회 1988 핵의학 분자영상 Vol.22 No.1

In Graves' disease, changes in orbital tissue and structure are casued by inflammatory infiltation, which induces increase of capillary permeability and breakdown of blood-tissue barriers. Using the uptake of Tc-99m-DTPA in inflammatory lesion, Eye/Brain radioactivity ratios in brain scintigraphy were evaluated in 15 normal controls and 40 Graves' patients. The results were as follows; 1) Eye/Brain radioactivity ratio was significantly higher in Graves' ophthalmopthy group than in control group (p〈0.005). 2) In Graves' ophthalmopathy, Eye/Brain radioactivity ratio was significantly higher in active (progressive) group than in inactive (non-progressive) group (p〈0.05). 3) There was no correlation between class of ATA classification of Graves' ophthalmopathy and Eye/Brain radioactivity ratio. 4) There was no correlation between Eye/Brain radioactivity ratio and serum activity of TBII. In conclusion, Eye/Brain radioactivity ratio using Tc-99m-DTPA brain scintigraphy may be useful to determine the activity of Gves' ophthalmopathy and whether treatment of Graves' ophthalmopathy is necessary or not.

2005학년도 부산교육대학교 신입생 입학전형 방안

성병창,이경애,백대현 부산교육대학교 초등교육연구소 2004 초등교육연구 Vol.19 No.-

The goal of this paper is to develop a plan for the 2005 BNUE's annual regular recruitment. We develop a plan in which BNUE seeks to maximize the quality of prospective students based on the main issues in relation to 7th curriculum and the College Scholastic Ability Test (CSAT). We deduce the following three suggestions regarding to reflect the CAST score. (ⅰ) BNUE reflects the standard scores of Verbal (Korean) by 27% of the CSAT and Foreign Language (English) by 26% of the CSAT. (ⅱ) BNUE reflects the standard score of Mathematics by 27% of the CSAT, but by adding at least an additional 5.5% of the mathematics standard score if Mathematics type 'Ga' is taken instead of type 'Na'. (ⅲ) BNUE reflects the best three (two in case of Occupational Inquiry) standard scores of Inquiry score by 20% of the CSAT by choosing higher standard score if subjects' ranking percentages are equal.

Genetic Polymorphisms of Blood Serum Proteins and Enzymes in Holstein Cattle

Sang, B. C 韓國酪農學會 1996 韓國酪農學會誌 Vol.18 No.2

산란계의 연령과 면역주기에 따른 난황 중의 Streptococcus mutans 특이 항체 함량

성기승,김영붕,노정해,이남형,한찬규,손동화 한국축산학회 1999 한국축산학회지 Vol.41 No.5

Streptococcus mutans-specific IgY content change, laying rate, egg weight and weight change were measured for 17-week and 30-week old hens. Vaccinations with Streptococcus mutans were made two times(eight week interval), three times(four week interval) and five times(two week interval), respectively. It was observed that the laying rate of vaccinated hens was likely lower than that of non-vaccinated group. No effect on body weight by vaccination was found out. Egg weight did not show a certain tendency by vaccination. Anti-S. mutans IgY started to be detected two weeks after the lst vaccination for 30-week old hens. It was not detected for non-vaccinated group. The antibody activity was consistently detected after 8 weeks from the last vaccination. The measurement of total IgY and S. mutans-specific IgY in the egges from vaccinated hens revealed that IgY tended to increase with the number of vaccination. S. mutans-specific IgY content of five-time vaccinated l7-week hens was much higher than that of 30-week old hens. To obtain steady amount of specific IgY, multiple vaccination with two week interval was recommended.