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사료내 지방산 조성이 등지방 두께 및 돼지 혈액의 지방산 조성에 미치는 영향
성기승,이무하,김윤지,강통삼,신대근,권찬호 한국축산학회 1999 한국축산학회지 Vol.41 No.1
The effects of feeding isofat (8%) diets with different ratios of ω-3, ω-6 and ω-9 unsaturated fatty acids (UFA) were investigated to evaluate the accumulation of UFA in blood and growth rate of pigs. Landrace x Yorkshire x Large white crossbred pigs(36 heads) weighing 70㎏ on the average were randomly assigned to 5 treatments and a control. Each of animals was fed a diet with the different ω-fatty acid ratio for about 46 days, reaching 110㎏ of body weight. Dietary fat content was 8% and ω-3, ω-6 and ω-9 UFA ratios were divided to 5 groups(1:1.5:2, 1:2:3, 1:1:1, 1:2:1, 1:3:1). Average body weight gain and feed efficiency of the pigs were not influenced by the dietary ω-UFA ratio. But back fat thickness was influenced by the ratios in the end of the point(P$lt; 0.05). In the C treatment fed diet that the ratio of ω-3, ω-6 and ω-9 UFA was 1 : 1 :1, back fat thickness was the thickest as 21.00±2.10mm. But in D treatment fed diet containing ω-3, ω-6 and w-9 UFA was 1:2:1, back fat thickness(17.40±1.52mm) was thinner than other treatments. Correlations of saturated fatty acid(SFA), polyunsaturated fatty acid(PUFA), monounsaturated fatty acid(MUFA), ω-3, ω-6 and ω-9 fatty acid were examined between blood and diet. Positive correlation was shown in PUFA, ω-3 and ω-6 fatty acid ratio between blood and diet(P$lt; 0.01) and in PUFA/SFA between blood and diet(P$lt; 0.05). In this study, as the PUFA increased in diet the MUFA decreased in blood and as the ω-3 fatty acid increased in diet the ω-6 fatty acid decreased in blood because ω-3 fatty acid increased in blood.
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산란계의 연령과 면역주기에 따른 난황 중의 Streptococcus mutans 특이 항체 함량
성기승,김영붕,노정해,이남형,한찬규,손동화 한국축산학회 1999 한국축산학회지 Vol.41 No.5
Streptococcus mutans-specific IgY content change, laying rate, egg weight and weight change were measured for 17-week and 30-week old hens. Vaccinations with Streptococcus mutans were made two times(eight week interval), three times(four week interval) and five times(two week interval), respectively. It was observed that the laying rate of vaccinated hens was likely lower than that of non-vaccinated group. No effect on body weight by vaccination was found out. Egg weight did not show a certain tendency by vaccination. Anti-S. mutans IgY started to be detected two weeks after the lst vaccination for 30-week old hens. It was not detected for non-vaccinated group. The antibody activity was consistently detected after 8 weeks from the last vaccination. The measurement of total IgY and S. mutans-specific IgY in the egges from vaccinated hens revealed that IgY tended to increase with the number of vaccination. S. mutans-specific IgY content of five-time vaccinated l7-week hens was much higher than that of 30-week old hens. To obtain steady amount of specific IgY, multiple vaccination with two week interval was recommended.
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성기승,윤칠석,이남형,한찬규,이복희 한국축산학회 2000 한국축산학회지 Vol.42 No.1
The experiment was conducted to determine the effects of various commercial feed additives on specific components in shell egg and to develop a new type of brand egg. Forty-four week old ISA Brown layers were randomly assigned to 8 treatments. Hens of each treatment were 300 and treatment was replicated 3 times. Experimental period was 10 weeks. The 8 treatments were as follows: astarich^ⓡ 2%(B), astarich^ⓡ 5%(C), chitin+chitosan 2%(D), omega-3 powder 2%(E), pyrogreen 1%(F), greenpia 0.2%(G), hydrogenated soy oil 3%(H) and commercial layer feed(A). Eggs were collected at day 0, 7, 14, 40 and 70 for chemical analyses. Chemical analyses were done for vitamins A and E, cholesterol and fatty acid profile of egg yolks. During the experimental period, the contents of vitamin A increased overall. Astarich^ⓡ 5%(C) group showed the highest amounts of vitamin A (11.14±3.93 ㎍/g yolk) whereas commercial layer feed goup(A) showed the lowest amounts of vitamin A(8.65±1.97 ㎍/g yolk). Vitamin E contents were significantly different among treatments at day 7, 40, and 70 (p$lt;0.05). Mean value of vitamin E (99.66±8.30 ㎍/g yolk) was the highest in omega-3(E) group and the lowest in pyrogreen (F) group (53.49±11.36 ㎍/g yolk). Yolk cholesterol contents tended to increase, although the fluctuation existed somewhat depending on the day of measurement. The highest value of yolk cholesterol(11.71±0.50㎎/g yolk) was observed in astarich^ⓡ 2%(B) group and the Lowest value(9.45±2.32㎎/g yolk) in pyrogreen (F) group. The mean compositions of yolk fatty acids during the experimental period were 37.3∼39.6% for saturated fatty acids(SFA), 39.3∼41.3% for monounsaturated fatty acids(MUFA), 16.2∼18.9% for omega-6 fatty acids and 2.20∼5.36% for omega-3 fatty acids. In general, the concentrations of SFA and omega-6 fatty acids decreased, while those of MUFA tended to increase. On the other hand, eicosapentaenoic acid(C_(20:5) ω6, EPA) and docosapentaenoic acid(C_(22:5) ω3, DPA) were detected only in astarich^ⓡ 5%(B) and omega-3(E) groups where the docosahexaenoic acid(C_(22:6) ω3, DHA) concentration was over twice higher than those of other group. The study proved both astarich^ⓡ and omega-3 powder were suitable to produce brand eggs specially high in certain components.
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늙은 호박, 율무, 꾸지뽕잎 혼합조성물이 고지방식이를 급여한 흰쥐의 내장지방, 배변량 및 혈청지질농도에 미치는 영향
이경원,성기승,김성수,이옥환,이복희,한찬규,Lee, Kyoung-Won,Sung, Ki-Seung,Kim, Sung-Soo,Lee, Ok-Hwan,Lee, Bog-Hieu,Han, Chan-Kyu 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4
고지방식이(D12492)를 5주 동안 흰쥐에게 급여한 다음 고지방식이에 늙은 호박(Cucurbita moschata), 율무(adlay seed), 꾸지뽕(Cudrania tricuspidata) 분말조성물(1:1:1) 첨가식이를 5주 동안 급여한 결과는 다음과 같다. 실험기간동안 증체량은 혼합조성물 10% 첨가군(B)을 제외하고, 혼합조성물의 첨가 수준이 높을수록 감소 경향을 나타내었다. 식이섭취량은 차이가 없이 혼합조성물의 첨가 수준이 높을수록 다소 증가한 반면, 식이효율은 혼합조성물의 첨가 수준이 높을수록 다소 감소하였다. 내장지방 중 신장주변지방패드(RFP) 무게는 고지방식이대조군(F)보다 혼합조성물 30% 첨가군(E)이 통계적으로 낮았고, 정소상체지방패드(EPF) 무게는 F군보다 혼합조성물 20% 첨가군(D)이 유의하게 낮았다(p<0.05). 배변량의 경우, 실험 전기간(10주) 고지방식이대조군(F)은 실험1기와 실험2기의 배변량이 차이가 없었고, 혼합조성물의 경우 첨가 수준이 높을수록 F군보다 통계적으로 유의하게 증가하였다(p<0.05). 소장길이는 실험군 간에 유의한 차이가 없이 혼합조성물 10%, 15% 및 20% 첨가군(B, C, D)이 F군보다 다소 긴 것으로 나타났고, 대장길이는 F군과 20% 첨가군(D) 간에 통계적으로 유의한 차이가 있었다(p<0.05). 혈청지질중 TC, HDL- 및 LDL-콜레스테롤 농도는 유의적인 차이가 없었고, TG 농도는 혼합조성물 10%(B)와 15% 첨가군(C)이 낮았고, F군과 다른 첨가군은 높았다. 혈당농도는 혼합조성물첨가군이 F군보다 통계적으로 유의하게 낮았다(p<0.05). 혈구세포(CBC)중 RBC와 WBC는 혼합조성물 30% 첨가군(E)이 F군보다 통계적으로 높았고, Hct와 Hb 함량은 E군이 F군보다 높았으며, 혈소판(Platelet) 수는 혼합조성물첨가군(A~E)이 F군보다 통계적으로 유의하게 높았다(p<0.05). 이상에서 고지방식이를 급여한 흰쥐에게 늙은 호박, 율무, 꾸지뽕잎 분말조성물첨가식이를 급여했을 때 체지방량 저하, 배변량 증가, 혈청중 TG 감소 및 혈당치의 유의한 감소작용이 있는 것으로 나타나서, 앞으로 이들 혼합조성물의 적절한 처방으로 체지방 억제, 배변활성 및 지질개선을 위한 소재화 기술개발이 필요한 것으로 판단된다. This study investigated the effects of the diet with Cucurbita moschata (CM), adlay seed (AS), and Cudrania tricuspidata leaf (CTL) mixed-powder on the visceral fat, fecal amount, and serum lipid profiles in rats fed a high-fat diet (HFD). The mixed ratio of CM, AS, and CTL as a functional food ingredient was 1:1:1 on a weight basis. Male Sprague-Dawley rats were fed a high-fat diet (D12492; 45 kcal fat) for 5 weeks during the first phase. In the second phase, which lasted 5 weeks, the rats were divided into six experimental groups. The groups were: a high-fat diet group as a control during the 10 weeks of experimental period; and a high-fat diet with 5, 10, 15, 20, and 30% mixed-powder supplement group. The adipose tissue (RFP, EFP) weights were significantly decreased in the 20% and 30% mixed-powder supplement groups than in the HFD group (p<0.05). The fecal weight produced by the 15%, 20%, and 30% mixed-powder supplement groups was significantly increased during the second phase compared to the first phase (p<0.05). In terms of serum lipids, TG (triglyceride) content was decreased in the 10% and 15% mixed-powder supplement groups. The blood glucose levels were significantly decreased in all experimental diet supplement groups than in the HFD group (p<0.05). The findings show that the high supplement groups could improve the visceral fat accumulation, fecal amount, and blood glucose content compared to the HFD group.
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