소에서의 유전적 다형의 이용

신형두 韓國受精卵移植學會 1995 한국동물생명공학회지 Vol.10 No.1

Several kinds of analytic methods for genetic polymorphism in cattle, including bovine blood typing, PCR-RFLP, BoLA and microsatellite typing were described. A few respect to consider for choosing method for actual application of genetic polymorphism were emphasized. The probability of relationship between characteristics and gene concerned, repetibility and easiness of methods applied and the possibility of clarification for segregation pattern should be deliberated.

표준항혈청 생산 및 한우 ( Bos taurus coreanae ) 의 혈액형에 관한 연구

신형두,윤충근,신언익,양일석,권종국 ( H . D . Shin,C . K . Yoon,U . I . Shin,I . S . Yang,J . K . Kwun ) 한국축산학회 1993 한국축산학회지 Vol.35 No.4

These studies were carried out to produce standard antisera for the serological blood typing of cattle and to clarify the genetic characteristics of Korean Native Cattle. For the serological blood typing, thirty-three standard antisera(reagents) were produced by iso-immunizations and absorptions with two hundreds animals. Seven hundreds and forty-two Korean Native Cattle were bloodtyped by those antisera. Also the phenogroups (alleles) in each blood group system were investigated. Two hundreds animals were bloodtyped with standard antisera which introduced from bovine bloodtyping laboratory, Saskatoon, Canada and used far isoimmuniz anon to produce immunesera. Thirty-two reagents(A1, A2 and Z` of A system; B, C, I1, Y2, A`1, A`2, B`, D`, E`2, E`3, I` and J` of B system; C1, C2, X1, X2, R1, R2, W and L` of C system; F and V of I` system; J of J system; S, H`, U1, U`1, U` and U$quot; of S system: Z of Z system) were produced by iso-immnunizations and absorptions. And J reagent was seperated from natural serum. In ISAG international comparison test in 1991, the specificities of these thirty-three antisera were compared with those of other laboratory. In A blood system, four kinds of phenogroups were appeared. The gene frequency of A1Z` phenogroup, which has been characteristically reported in Indian cattle breed, was 0.206. In B blood system, thirty-five kinds of phenogroups were appeared and showed similar gene frequencies in each other. In C blood system, sixteen kinds of pheuogroups were appeared. The gene frequency of X2 phenogroup was the highest(0.300). In F blood system, three kinds of alleles were appeared. The gene frequency of $quot;-$quot; allele which has been characteristically reported in Indian cattle breed, was 0.213. In S blood system, ten kinds of phenogroups were appeared. The gene frequency of $quot;-$quot; and G$quot; phenogroups were relatively high. In J and Z blood system, the gene frequency of J and Z alleles were 0.432 and 0.600.

Novel Promoter Polymorphism in RUNX2 Is Associated with Serum Triglyceride Level

신형두,전재필,박병래,배준설,남혜영,심성미,박경수,한복기 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.5

Much research evidence supports the hypothesis that chronic, low-grade inflammation related to innate immunity may play an important role in the pathophysiology of type 2 diabetes mellitus (T2DM). Runt-related transcription factor 2 (RUNX2; MIM# 600211) acts as a scaffold that controls the integration, organization, and assembly of nucleic acids. To examine whether the novel promoter variant in orkuO is associated with the risk of T2DM and related phenotypes, RUNX2-742G >T was genotyped in 378 T2DM patients and 382 normal controls recruited in the Korean T2DM Study. Statistical analysis revealed that RUNX2-742G >T was associated with serum triglyceride level (TG) in nondiabetic controls, although it was not associated with the risk of T2DM. Individuals who carry T/T, T/G, and G/G genotypes had the highest (2.061 ± 0.20), intermediate (2.01 ± 0.19), and the lowest (1.97 ± 0.18) levels of log [TG (mmol/l)] (P = 0.007), respectively. Our data on this important variant of orkuO suggest that lipid metabolism might be affected by genetic polymorphisms in the promoter region.

No association of TF gene polymorphisms with hepatitis B virus Clearance and hepatocellular carcinoma occurrence in a Korean population

신형두,김용하,김정현,박태준,이진솔,Charisse Flerida Pasaje,배준설,박병래,정현섭,이효석,김윤준 한국유전학회 2011 Genes & Genomics Vol.33 No.3

Development of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). The transferrin (TF) gene encodes a blood plasma protein that delivers iron ion in the body. The iron uptake level has been shown to be different in HCC tumor regions, indicating a possible association between iron uptake level and HCC. To investigate whether genetic polymorphisms of TF are related with HBV clearance and/or HCC occurrence, we sequenced genomes of 24 individuals and detected 37 variants. Subsequently, eight single nucleotide polymorphisms (SNPs) in TF including 4 in the promoter region,1 in 5’UTR and 3 in coding regions were selected and genotyped in 1,101 Korean subjects including 428 spontaneously recovered (SR) patients as controls and 673 chronic carriers (CC) as cases. Results of logistic analyses adjusted for age and gender, however, revealed no significant associations of polymorphisms and haplotypes in the TF gene with HBV clearance and HCC occurrence (P > 0.05). Since age of HBV infection is a risk factor in progression to HCC, further Cox proportional regression analysis for age of HCC as a relative hazard was performed; but no association between TF polymorphisms and onset age of HCC was found (P > 0.05). Although TF gene polymorphisms have been previously re-ported to be associated with various diseases, our findings indicate that genetic variations of the TF gene do not influence HBV clearance and HCC occurrence in a Korean population.

Common interleukin 10 polymorphism associated with decreased risk of tuberculosis

신형두,Lyoung Hyo Kim,정현섭,In Hee Lee,Seung Kyu Park,Byung Lae Park1, 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2

Interleukin 10 (IL10) is a powerful TH2-cel cyto-kine that inhibits lymphocyte replication and secretion of inflammatory cytokines. The genetic associations of polymorphisms in IL10 with clini-cal manifestations of tuberculosis (TB) were exa-mined in a large number of patients with clinical TB infection (n = 459) and normal controls (n = 871). One comon promoter SNP (IL10 -592 A>C) was found to be significantly asociated with decreased risk of TB manifestation. The fre-quency of the "C"-bearing genotype was higher in normal controls than in patients with clinical TB infection (P = 0.005, OR = 0.69). A sumary of the genetic effect of IL10 -1082 A>G, the other nearby promoter SNP, in other ethnic groups is also presented.

동종면역에 의한 소 혈액형 표준혈청의 생산

신형두,한호재,이국경,강동묵,양일석,권종국 韓國受精卵移植學會 1988 한국동물생명공학회지 Vol.3 No.1

This study was carried out to produce the antisera for the blood typing in cattle. Blood types of eighty cattle were previously determined by 56 kinds of internationally standardized antisera from Japan. The donorrecipient animal arrangements were determined according to tile previously determined blood types of animals by the computer program SS-l for efficient production of antisera. Six kinds of standard antisera, H,B', 12, C2, Z, U2, were produced by isoimmunirzation and absorption methods.

유전과 환경

신형두,Shin, Hyeong-Du 한국유전체학회 2006 유전체소식 Vol.6 No.3

Host Genetic Epidemiology by Single Nucleotide Polymorphism (SNP) Analysis

신형두 ( Sin Hyeong Du ) 대한신장학회 2002 추계학술대회 초록집 Vol.21 No.2

한우 ( Bos taurus coreanae ) 의 혈액단백다형에 관한 연구

신형두(H . D . Shin),신언익(U . I . Shin),양일석(I . S . Yang),권종국(J . K . Kwun) 한국축산학회 1993 한국축산학회지 Vol.35 No.3

These studies were carried out to clarify the genetic characteristics of Korean Native Cattle. To examine the biochemical polymorphisms of eight protein loci, polyacrylamide and starch gel electrophoresis methods were used. Gene frequencies of alleles in hemoglobin locus(Hb) were 0.870(Hb A) and O.130(Hb B) which was rarely reported in European cattle breed. Gene frequencies of alleles in albumin locus(Alb) were 0.997(AIb A) and 0.003(Alb B) which was rarely reported in European cattle breed. Gene frequencies of alleles in amylase t locus (Am I) were 0.307(Am I B) and 0.693(Am I C). Gene frequencies of alleles in carbonic anhydrase locus(CA) were 0.065(CA F) and 0935(CA S). Gene Irequencics of alleles in ceruloplasmin locus(Cp) were O.s38(Cp A) and 0.662(Cp C). Gene frequencies of alleles in post transferrin-2 locus(pTf-2) were 0.692(pTf-2 F) and 0.30 8(pTf-2 S). Gene frequencies of alleles in post-albumin locus(pAlb) were 0.580(pAlb F) and 0.420(pAlb S). Gene frequencies of alleles in transferrin locus(Tf were 0.268(Tf A), 0.234(Tf D1), 0.247(Tf D2), 0.235(Tf E), 0.017(Tf K) and 0.001(Tf Y), respectively. The two transferrin variants have been found in this studies and nomenclatured as Tf K and Tf Y. The heredity of Tf K allele were reported at first.

한우혈액형의 phenogroup 과 아형관계에 관한 연구

신형두(H . D . Shin),신언익(U . I . Shin),양일석(I . S . Yang),권종국(J . K . Kwun) 한국축산학회 1990 한국축산학회지 Vol.32 No.9

This experiment was carried out to clarify the phenogroups and subtype relationships of bloodtype antigens appeared in Korean Native Cattle. Fifty-two standard antisera(reagents) which were standardized by International Society for Animal Blood Group Research were introduced for bloodtyping of 213 Korean Native Cattle. And computer program II-2 and I-2 were used to find phenogroups. 1. In A system, 4 kinds of phenogroups; A₁, A₂ A₁Z` and $quot;-$quot; were appeared in Korean Native Cattle and A₁ Phenogroup showed the highest gene frequency(0.5065). Z`-A₁-A₂ linear subtype relationship was proved in Korean Native Cattle. 2. In B system, 49 kinds of phenogroups were appeared in Korean Native Cattle. And B-K, linear subtype relationships and A`₁-A`₂ A`_x, non-linear subtype relationship were approved. In G₁-G₂-G_x non-linear subtype relationship, G₂ antigens without G₁ and G_x antigens were appeared. 3. In C system, C₁-C₂. R₁-R₂ linear subtype relationship and X₁-X₂-X` non-linear subtype relationship were appeared in Korean Native Cattle. 4. In F system, 7 kinds of phenogroups : F, V₁, V₂, FN`, V₁N`, FV₂N` and $quot;-$quot; were appeared in Korean Native Cattle and FV₂N` phenogroup which had not emerged in other species of East Asian cattle showed gene frequency of 0.0013. In V₁-V₂ linear subtype relationship, V₁ antigens without V₂ antigens were appeared in Korean Native Cattle. 5. In S system, 5 kinds of phenogroups : H`U₁, SH`, H`, U` and $quot;-$quot; were appeared in Korean Native Cattle and H` and U` phenogroups showed relatively high gene frequencies, 0.2523 and 0.2614, respectively. U₁-U₂-U` non-linear subtype relationship was appeared in Korean Native Cattle. 6. In J, L, Z and R`-S` system, two kinds of alleles were appeared in Korean Native Cattle, respectively. The gene frequencies of J. L, Z and R`-S` alleles were 0.1279, 0.0383, 0.4827 and 0.0024, respectively.